Lysine 2-hydroxyisobutyrylation levels determined adipogenesis and fat accumulation in adipose tissue in pigs.

BACKGROUND: Excessive backfat deposition lowering carcass grade is a major concern in the pig industry, especially in most breeds of obese type pigs. The mechanisms involved in adipogenesis and fat accumulation in pigs remain unclear. Lysine 2-hydroxyisobutyrylation (Khib), is a novel protein post-translational modification (PTM), which play an important role in transcription, energy metabolism and metastasis of cancer cells, but its role in adipogenesis and fat accumulation has not been shown. RESULTS: In this study, we first analyzed the modification levels of acetylation (Kac), Khib, crotonylation (Kcr) and succinylation (Ksu) of fibro-adipogenic progenitors (FAPs), myogenic precursors (Myo) and mesenchymal stem cells (MSCs) with varied differentiation potential, and found that only Khib modification in FAPs was significantly higher than that in MSCs. Consistently, in parallel with its regulatory enzymes lysine acetyltransferase 5 (KAT5) and histone deacetylase 2 (HDAC2) protein levels, the Khib levels increased quadratically (P < 0.01) during adipogenic differentiation of FAPs. KAT5 knockdown in FAPs inhibited adipogenic differentiation, while HDAC2 knockdown enhanced adipogenic differentiation. We also demonstrated that Khib modification favored to adipogenic differentiation and fat accumulation by comparing Khib levels in FAPs and backfat tissues both derived from obese-type pigs (Laiwu pigs) and lean-type pigs (Duroc pigs), respectively. Accordingly, the expression patterns of KAT5 and HDAC2 matched well to the degree of backfat accumulation in obese- and lean-type pigs. CONCLUSIONS: From the perspective of protein translational modification, we are the first to reveal the role of Khib in adipogenesis and fat deposition in pigs, and provided new clues for the improvement of fat accumulation and distribution as expected via genetic selection and nutritional strategy in obese-type pigs.

Translation and psychometric evaluation of the Chinese version of the Nursing Time Management Scale.

Background: Reasonable and effective time allocation can promote the improvement of medical care service quality. This study aimed to translate, cross-culturally adapt and validate the Chinese Nursing Time Management Scale (NTMS). Methods: Using a cross-sectional survey, 345 clinical nurses were selected from June to September 2023 for a general information questionnaire and Nursing Time Management Scale (NTMS) study. Item analysis, exploratory factor analysis and validation factor analysis were used to verify the reliability and validity of the Chinese version of Nursing Time Management Scale. Results: The Chinese version of the Nurses' Time Management Competency Scale includes 17 entries in 3 dimensions: planning activities and setting goals, coordinating activities and procedures, and organizing nursing activities. The Cronbach's alpha coefficient for the total scale was 0.966. Exploratory factor analysis showed that the cumulative variance contribution of the three male factors was 97.44%. Conclusion: The NTMS has acceptable validity and reliability and can be used to evaluate the nursing time management skills of Chinese clinical nurses.

Analysis of patients' privacy and associated factors in the perioperative period.

Background: Healthcare professionals recognize how to protect patient privacy in order to effectively reduce the occurrence of conflict between the two parties. Therefore, understanding the protection of patient privacy during the perioperative period and the relevant factors affecting privacy is essential to improving healthcare delivery. Methods: This was a cross-sectional survey of a convenience sample of 400 perioperative patients. General demographic information, the perioperative privacy scale (PPS), and the Amsterdam preoperative anxiety and information scale (APAIS) were used for the survey. And factors affecting patient privacy were investigated by ANOVA or t-test analysis, Pearson correlation analysis, and linear regression models. Results: This study found that perioperative patient privacy satisfaction scores were (53.51 ± 12.54). The results of the univariate analysis showed that factors affecting privacy satisfaction included gender, age, and the number of surgeries (p < 0.05). Preoperative anxiety and Information Needs Scale was negatively associated with the perioperative patient privacy satisfaction (r = -0.807, p < 0.01). Further analysis was performed using linear regression models to finally obtain five factors affecting perioperative patient privacy: gender, age, anesthesia modality, the number of surgeries, and the Amsterdam preoperative anxiety and information. Conclusion: Healthcare professionals working in healthcare facilities need to be aware of the sensitivity of different populations to privacy when protecting patient privacy. Patients' preoperative anxiety and information need status affect privacy satisfaction. This will mean that healthcare professionals will be able to identify key privacy concerns early and take appropriate action.

Psychometric evaluation of the Chinese version of motivation for nursing student scale (MNSS): A quantitative and cross-sectional design.

BACKGROUND: Academic motivation is the psychological factor that promotes learning activities. The persistence of learning activities in nursing education is directly influenced by academic motivation. However, there are currently no specialist instruments for nursing students in China. OBJECTIVE: To translate the Motivation for nursing student scale (MNSS) into Chinese and evaluate its validity and reliability among nursing students in China. DESIGN: A quantitative and cross-sectional design. SETTING: The survey was conducted at a medical university in Jinzhou, China between March and May 2022. PARTICIPANTS: A total of 688 Chinese nursing students were surveyed by questionnaire in this study. METHOD: The Chinese version of MNSS adopted Brislin's transition model and conducted expert consultations to validate the facial validity and testing of the transition version. Reliability and validity were tested using exploratory factor analysis, confirmatory factor analysis, and internal consistency reliability. RESULTS: Nursing experts confirm the high content validity of the Chinese version of the 20-item scale. An exploratory factorial analysis revealed a four-factor solution, with a total variance of 64.1% and confirmatory factor analysis results showed a satisfactory fit (χ2/df=2.738, RMSEA =0.073, SRMR = 0.0719, CFI = 0.914, IFI = 0.915, NFI =0.872, RFI =0.849,). Cronbach's alpha coefficient for the scale was 0.869, and the split-half reliability is 0.727. CONCLUSION: The Chinese version of MNSS has satisfactory reliability and validity and is a reliable instrument to assess the academic motivation of Chinese nursing students.

Analysis of the current status of rehabilitation motivation and its influencing factors in older adults with stroke: a cross-sectional study.

Objective: Among stroke patients, exercise adherence is an important factor in reducing the rate of physical disability and mortality. Rehabilitation exercise after stroke is a safe and effective measure to restore normal body functions, but the analysis of factors influencing patients' motivation for rehabilitation is not well established. Therefore, this study will explore the influencing factors of rehabilitation motivation in older adults with stroke so as to reduce the disability rate of stroke. Method: A convenience sampling method was used to study 350 patients in a stroke ward of a tertiary care hospital in Jinzhou, Liaoning Province. Patients' general demographic data, Multidimensional Scale of Perceived Social Support (PSSS), Questionnaire of Exercise Adherence (EAQ), Tampa scale of kinesiophobia (TSK-11), and Motivation in stroke patients for rehabilitation scale (MORE) were assessed. ANOVA or t-test analysis, correlation analysis, and linear regression analysis were used to explore the factors influencing the motivation for rehabilitation in older adults with stroke. Results: The results showed that stroke patients' motivation for rehabilitation was at a moderate level. Perceived social support, exercise adherence, and stroke motivation were positively correlated (r = 0.619, p < 0.01; r = 0.569, p < 0.01), and kinesiophobia was negatively correlated with stroke motivation (r = -0.677, p < 0.01). Time of stroke, location of the lesion, perceived social support, exercise adherence, and kinesiophobia are influential factors affecting patients' motivation to recover. Conclusion: In the rehabilitation program for older adult patients with stroke, healthcare providers should specify targeted rehabilitation measures according to the different degrees of the patient's condition, so as to improve the effectiveness of rehabilitation medical implementation.

Cross-cultural adaptation and validation of the Motivation in Stroke Patients for Rehabilitation Scale in Chinese hospitalized older adults with stroke.

OBJECTS: To translate, cross-culturally adapt, and validate the Motivation in Stroke Patients for Rehabilitation Scale (MORE) questionnaire in Chinese hospitalized older adults with stroke. METHODS: The Chinese version of the MORE was produced following Brislin's guidelines. The psychometric properties of the MORE were evaluated among 420 eligible patients. RESULTS: The Cronbach's alpha coefficient of the Chinese version of the MORE scale was 0.983, and the content validity index (S-CVI) was 0.94, with good reliability. Exploratory factor analysis showed a single-factor structure that explained 78.01% of the total variance, and the confirmatory factor analysis model had a good fit index (X2/df=2.97; NFI =0.93; CFI=0.96; TFI=0.95; IFI=0.96). CONCLUSION: The MORE presented acceptable validity and reliability and could be used in Chinese hospitalized older adults with stroke.

The effect of pain social support on kinesiophobia in older patients with rheumatoid arthritis: The mediating role of self-perceived burden.

OBJECTIVES: This study would investigate the role of self-perceived burden in mediating the relationship between pain social support and kinesiophobia. METHODS: A convenience sampling method was used to survey 386 older patients with rheumatoid arthritis in a hospital in Liaoning Province. They were surveyed using the general demographic questionnaire, pain social support scale (SPQ), self-perceived burden scale (SPBS), and tampa scale of kinesiophobia(TSK-11). RESULTS: Pain social support had a negative correlation with kinesiophobia(r=-0.336, p < 0.01). The self-perceived burden was positively correlated with kinesiophobia(r = 0.583, p < 0.01). The mediating effect of self-perceived burden accounted for 43.40% of the total effect. CONCLUSIONS: The study had shown the mediating role of self-perceived burden to be beneficial in reducing kinesiophobia in older rheumatoid arthritis patients.

Pilot-scale binder jet 3D printing of sustained release solid dosage forms.

The additive nature and versatility of 3D printing show great promise in the rapid prototyping of solid dosage forms for clinical trials and mass customization for personalized medicine applications. This paper reports the formulation and process development of sustained release solid dosage forms, termed "printlets", using a pilot-scale binder jetting (BJT) 3D printer and acetaminophen (APAP) as the model drug. With the inclusion of hydroxypropyl methylcellulose (HPMC) as a release retardant polymer in the print powder, the drug release time of APAP increased considerably from minutes to hours. However, given the swelling propensity of HPMC, a thicker layer of powder must be laid down during printing to avoid any shape distortion of the printlets. For a fixed print volume, the level of binder saturation (i.e., ratio between the liquid binder and powder in the as-printed sample) is inversely proportional to the thickness of the spread powder layer. An increase in the spread powder layer inadvertently resulted in a lower level of binder saturation and consequently weaker printlets. By increasing the level of binder saturation with jetting from more print heads, the mechanical strength of printlets containing 18% HPMC was successfully restored. The resultant printlets have a drug release time of 3.5 h and a breaking force of 12.5 kgf that is comparable to the fast-disintegrating printlets containing no HPMC and surpasses manually pressed tablets with the same HPMC content.

Factors associated with kinesiophobia in Chinese older adults patients with osteoarthritis of the knee: A cross-sectional survey.

OBJECTIVES: To identify important determinants of kinesiophobia in older adults with osteoarthritis of the knee bassed on demographics, social support and pain, and self-perceived burden factors. METHODS: A cross-sectional survey of 304 older adults patients with knee osteoarthritis from two hospitals in Jinzhou, Liaoning, China. General Demographic Questionnaire, the Social Support and Pain Scale (SPQ), the Self-Perceived Burden Scale (SPBS), and the Tampa Kinesiophobia Scale (TSK-11) were used to collect the data. RESULTS: The results showed that the prevalence of kinesiophobia in older adults with osteoarthritis of the knee was 57.89%. Marital status, education, knowledge of the condition, pain level, SPQ, and SPBS levels were significant determinants of kinesiophobia, which together explained 43.2% of the variance. CONCLUSIONS: The prevalence of kinesiophobia in older adults with knee osteoarthritis is very high. Health care workers should take early intervention measures to improve social support and pain and reduce the self-perceived burden, thereby promoting recovery from disease.