RESUMO
Cadmium (Cd) removal from soil is crucial as Cd enters the food chain and affect food safety, thus impose severe threaten to human health. We developed PPC@PC-Fe, a dual-functional core-shell sphere for efficient soil Cd reduction. The shell (PPC) was composed of encapsulated citric acid (CA) in a polylactic acid (PLA) and polyethylene glycol (PEG) network, which endows a function of activating Cd; and the core (PC-Fe) consisted of a polyacrylic acid/carboxymethyl chitosan (PAA/CMC) hydrogel with Fe3O4 nanoparticles to adsorb adjacent activated Cd. Upon water contact, the shell dissolved, releasing CA to activate soil Cd. Simultaneously, the swellable PC-Fe core absorbed water and expanded in size, promoting the disintegration of PLA in the shell, which triggered the automatic separation of core from shell, enabling the exposed PC-Fe core to rapidly adsorb Cd. Furthermore, the PC-Fe core can be magnetically removed after adsorption of Cd. Soil culture tests showed that 2 % PPC@PC-Fe reduced soil Cd from 6.009 mg/kg to 4.834 mg/kg in 10 days, with the acid-soluble Cd being the predominantly target to be activated and remove. This study demonstrates an effective stepwise activation and adsorption mechanism by a single carrier, with simple magnetic collection minimizing secondary pollution. It offers an innovative approach to the remediation of cadmium-contaminated sites in the field.
RESUMO
Antibiotic abuse is increasing the present rate of drug-resistant bacterial wound infections, producing a significant healthcare burden globally. Herein, we prepared a pH-responsive CMCS/PVP/TA (CPT) multifunctional hydrogel dressing by embedding the natural plant extract TA as a nonantibiotic and cross-linking agent in carboxymethyl chitosan (CMCS) and polyvinylpyrrolidone (PVP) to prompt wound healing. The CPT hydrogel demonstrated excellent self-healing, self-adaptive, and adhesion properties to match different wound requirements. Importantly, this hydrogel showed pH sensitivity and exhibited good activity against resistant bacteria and antioxidant activity by releasing TA in case of bacterial infection (alkaline). Furthermore, the CPT hydrogel exhibited coagulant ability and could rapidly stop bleeding within 30 s. The biocompatible hydrogel effectively accelerated wound healing in a full-thickness skin defect model by thickening granulation tissue, increasing collagen deposition, vascular proliferation, and M2-type macrophage polarization. In conclusion, this study demonstrates that multifunctional CPT hydrogel offers a candidate material with potential applications for infected skin wound healing.
Assuntos
Antibacterianos , Bandagens , Quitosana , Hidrogéis , Cicatrização , Quitosana/química , Quitosana/análogos & derivados , Quitosana/farmacologia , Quitosana/síntese química , Cicatrização/efeitos dos fármacos , Hidrogéis/química , Hidrogéis/farmacologia , Hidrogéis/síntese química , Animais , Concentração de Íons de Hidrogênio , Camundongos , Antibacterianos/farmacologia , Antibacterianos/química , Antibacterianos/síntese química , Povidona/química , Masculino , Staphylococcus aureus/efeitos dos fármacos , Materiais Biocompatíveis/farmacologia , Materiais Biocompatíveis/química , Materiais Biocompatíveis/síntese química , Pele/efeitos dos fármacos , Pele/patologiaRESUMO
The selectivity of single-amino acid nanosensors is still not well understood. Herein, the factors that govern graphene-based nanomaterials for the selective detection of lysine are reported to guide the design of single-amino acid nanosensors. Graphene quantum dots (GQDs), nitrogen-doped GQDs (N-GQDs), and nitrogen/sulfur co-doped GQDs (N,S-GQDs) were used to sense lysine. The interaction mode and mechanism adjusted selectivity of the zero-dimensional graphene-based quantum dots to lysine ascribe to the solution behavior, molecular size, number of atoms as electron donors in graphene, and driving force. Being a basic amino acid, lysine is protonated with a positive charge below solution pH of 9. It adsorbed on the graphene-based quantum dots via electrostatic attraction, which blocked the internal charge transfer pathway inducing fluorescence enhancement at 420 nm. The protonated É-amine side of lysine is responsible for the course. The small diameter of the lysine of É-amine (<0.35 nm) favored its approach to the quantum dots, resulting in a fluorescence change, which could not be achieved with the larger arginine. The activated sites for interaction with lysine located at the edges of the layers of graphene to reach high selectivity. The N-GQDs and N,S-GQDs are much more sensitive to lysine than the GQDs because they contain nitrogen atoms as electron donors. They had similar linear detection ranges and detection limits, which suggested that the contribution of sulfur for lysine detection was minor. The results of this study provide new insights into the design of GQDs-based single-analyte nanosensors with high selectivity.