RESUMO
OBJECTIVE: To determine the level of DNA damage, both fragmentation and oxidative, in the sperm population used for intrauterine insemination (IUI) and intracytoplasmic sperm injection (ICSI) and its impact on fertilization and clinical pregnancy rates. DESIGN: A prospective clinical study. SETTING: A tertiary care fertility clinic. PATIENT(S): Couples undergoing ICSI (n = 48) and couples undergoing IUI cycles (n = 53). INTERVENTION(S): Assessment of both sperm DNA fragmentation using the TUNEL assay and oxidative DNA damage using the biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the samples prepared and used for insemination. MAIN OUTCOME MEASURE(S): Achievement of a clinical pregnancy. RESULT(S): Sperm DNA fragmentation and 8-OHdG were highly correlated (r = 0.55) and 8-OHdG was significantly lower in those who achieved a clinical pregnancy after IUI (8.9% vs. 20.2%). A threshold value of 11.5% 8-OHdG was identified as a useful predictor of IUI success. No differences were found in sperm DNA fragmentation or 8-OHdG between pregnant and nonpregnant couples in ICSI cycles. CONCLUSION(S): 8-Hydroxy-2'-deoxyguanosine, a biomarker of oxidative DNA damage highly correlated with sperm DNA fragmentation, in human sperm DNA has significant value in predicting the chance of a clinical pregnancy after IUI but not ICSI in assisted reproductive technology.
Assuntos
Dano ao DNA/fisiologia , DNA/metabolismo , Inseminação Artificial Homóloga/tendências , Estresse Oxidativo/fisiologia , Taxa de Gravidez/tendências , Injeções de Esperma Intracitoplásmicas/tendências , Espermatozoides/metabolismo , 8-Hidroxi-2'-Desoxiguanosina , Adulto , DNA/química , DNA/genética , Fragmentação do DNA , Desoxiguanosina/análogos & derivados , Desoxiguanosina/análise , Feminino , Humanos , Masculino , Gravidez , Estudos ProspectivosRESUMO
OBJECTIVE: To investigate the effects of repeated freezing and thawing on human sperm motility, vitality, and DNA integrity. DESIGN: A prospective clinical study. SETTING: Tertiary care fertility clinic. PATIENT(S): Twenty men presenting for infertility investigations. INTERVENTION(S): Each sample was subjected to three cycles of freezing and thawing both with and without washing steps and the addition of fresh cryoprotectant between each cycle. MAIN OUTCOME MEASURE(S): Percentage sperm DNA fragmentation, motility, and vitality before and following repeated freezing and thawing. RESULT(S): The percentage sperm DNA fragmentation rose significantly following each freeze-thaw cycle; however, samples that were not washed and to which fresh cryoprotectant was not added after each cycle fared significantly better than their washed counterparts. Both motility and vitality decreased steadily following each cycle but cell survival was significantly greater in the unwashed samples. CONCLUSION(S): In terms of the level of sperm DNA fragmentation, up to three cycles of freezing and thawing can be performed with a level of risk comparable to that following a single cycle of freezing and thawing. This is provided that samples are refrozen in their original cryoprotectant and not washed or altered in any way in between, and provided that they are separated by density gradient centrifugation or swim-up before use in assisted reproduction technology.
Assuntos
Criopreservação/métodos , Fragmentação do DNA , Congelamento , Preservação do Sêmen/métodos , Espermatozoides/fisiologia , Adulto , Centrifugação com Gradiente de Concentração/métodos , Centrifugação com Gradiente de Concentração/normas , Criopreservação/normas , Crioprotetores/farmacologia , Fragmentação do DNA/efeitos dos fármacos , Humanos , Infertilidade Masculina/fisiopatologia , Infertilidade Masculina/terapia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Preservação do Sêmen/efeitos adversos , Preservação do Sêmen/normas , Espermatozoides/efeitos dos fármacosRESUMO
OBJECTIVE: To investigate and compare seven different commercially available cryoprotectant media in terms of the DNA integrity of spermatozoa recovered after cryopreservation and separation using density gradient centrifugation (DGC). DESIGN: A prospective clinical study. SETTING: Tertiary care fertility clinic. PATIENT(S): Three hundred twenty men presenting for infertility investigations. INTERVENTION(S): Each sample was randomly assigned to one of seven commercially available cryoprotectants or to no cryoprotectant. MAIN OUTCOME MEASURE(S): Percentage sperm DNA fragmentation after cryopreservation and preparation using DGC. RESULT(S): The mean percentage fragmentation was significantly higher post-thaw and post-DGC; however, some patients (26.3%) demonstrated a lower percentage fragmentation post-thaw. No single cryoprotectant was identified as the best at preserving DNA integrity. The difference in fragmentation after thawing and DGC was found to be highly dependent on the prefreeze fragmentation. Motility was also significantly correlated with the difference in fragmentation post-thaw (r = -0.161). CONCLUSION(S): Neither the presence nor type of cryoprotectant affects the DNA integrity of spermatozoa after cryopreservation and DGC. Individuals with lower prefreeze fragmentation in DGC-prepared spermatozoa have larger increases in fragmentation and are less likely to exhibit lower levels of fragmentation post-thaw. The reverse effect observed in those with higher prefreeze fragmentation gives rise to a possible novel method of reducing fragmentation in sperm used for assisted reproductive technology treatment cycles without the need for testicular sperm retrievals.