Trigonelline alkaloid is effective in preventing doxorubicin-induced lung damage.

BACKGROUND: One of the most popular chemotherapy medications is doxorubicin (DOX), however it can have non-negligible damage. When the underlying mechanisms of damage are investigated, the most prominent pathways are oxidative stress, inflammation and apoptosis. AIM: We investigated the NF-κB/MAPK inflammatory pathway and cellular apoptosis to determine the efficacy of trigonelline alkaloid (TRIG) in preventing DOX-induced lung injury. METHODOLOGY: The study consisted of C, TRIG, DOX and TRIG+DOX groups. TRIG and TRIG+DOX groups received 50 mg/kg TRIG for 7 days. On day 8, DOX and TRIG+DOX groups received a single dose of 15 mg/kg DOX. RESULTS: Our results showed that apoptosis markers and inflammation were higher in the DOX group. In contrast, TRIG pretreatment partially suppressed apoptosis and decreased inflammation by blocking the activation of the MAPK/NF-κB pathway, lowering IL-6 levels, and protecting the lung from apoptotic cell death. CONCLUSION: Assessing TRIG's effectiveness in lung tissue injury, this study may be a crucial first step.

Evaluating the effects of Juglans regia L. extract on hyperglycaemia and insulin sensitivity in experimental type 2 diabetes in rat.

OBJECTIVE: The present study aimed to investigate the effects of Juglans regia (JR) extract on hyperglycaemia and insulin sensitivity. METHODS: Forty rats were divided into 4 groups: Control (C), Diabetes Control (DC), Diabetes + Juglans regia (D + JR) and Diabetes + Metformin (D + M). RESULTS: In the D + JR group, Fasting blood glucose (FBG) levels decreased from day 14 onwards. There was a significant decrease in plasma levels of Fibronectin Type III Domain Containing 5 (FNDC5) and adiponectin (ADP) in the DC group compared to the C group (p < .01, p < .001 respectively). In the D + JR group, there was a significant increase in plasma FNDC5 and ADP (p < .05), while the plasma Tumour necrosis factor-alpha (TNF-α) levels were decreased compared to the DC group (p < .001). CONCLUSIONS: In conclusion, the present study found that JR and its bioactive components alleviated insulin resistance by increasing ADP and FNDC5 and decreasing FBG in a rat model of streptozotocin (STZ) + nicotinamide (NAD)-induced type 2 diabetes (T2D).HighlightsJuglans regia extract increased insulin sensitivity.Juglans regia extract significantly reduced the level of fasting blood glucose.Juglans regia extract reduced TNF-α levels in rats with type 2 diabetes.Juglans regia extract prevent weight loss in type 2 diabetes.

Effects of Cinnamomum cassia extract on oxidative stress, immunreactivity of iNOS and impaired thoracic aortic reactivity induced by type II diabetes in rats

Type II diabetes is known to cause neuropathy, nephropathy and retinopathy. However, cardiovascular disorders associated with diabetes have been ignored. In traditional medicine, cinnamon (Cinnamomum cassia) bark has been used for its abilities to relieve fever, inflammation and chronic bronchitis. In the present study, the effect of Cinnamomum cassia extract (CN) on the thoracic aorta in an experimental type II diabetes model was investigated. In rats administered with nicotinamide + streptozotocin, significant endothelial dysfunction and oxidative stress were characterised by increased inducible nitric oxide synthase (iNOS) and decreased insulin/proinsulin levels. This impairment was prevented by administering 1000 mg/kg metformin or 500-1000-1500 mg/kg CN. CN administration attenuated the inflammatory response by decreasing the levels of malondialdehyde (MDA), Nitric oxide (NO) and increasing Glutathione peroxidase (GPx), glutathione (GSH). In addition, CN administration was shown to cause down-regulating effects on iNOS in thoracic aorta. These findings reveal that CN could prevent chronic complications of experimentally induced type II diabetes by attenuating inflammation, oxidant/antioxidant imbalance, and normalised contraction and relaxion responses in the thoracic aorta.