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1.
An. acad. bras. ciênc ; 90(1): 449-459, Mar. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-886902

RESUMO

ABSTRACT This study evaluated the chemical composition and antioxidant activity of fatty acids from the marine red algae Pterocladiella capillacea (S. G. Gmelin) Santelices & Hommersand 1997 and Osmundaria obtusiloba (C. Agardh) R. E. Norris 1991. The gas chromatography mass spectrometry (GC-MS) identified nine fatty acids in the two species. The major fatty acids of P. capillacea and O. obtusiloba were palmitic acid, oleic acid, arachidonic acid and eicosapentaenoic acid. The DPPH radical scavenging capacity of fatty acids was moderate ranging from 25.90% to 29.97%. Fatty acids from P. capillacea (31.18%) had a moderate ferrous ions chelating activity (FIC), while in O. obtusiloba (17.17%), was weak. The ferric reducing antioxidant power (FRAP) of fatty acids from P. capillacea and O. obtusiloba was low. As for β-carotene bleaching (BCB), P. capillacea and O. obtusiloba showed a good activity. This is the first report of the antioxidant activities of fatty acids from the marine red algae P. capillacea and O. obtusiloba.


Assuntos
Rodófitas/química , Ácidos Graxos/análise , Ácidos Graxos/química , Antioxidantes/análise , Antioxidantes/química , Valores de Referência , Análise de Variância , Sequestradores de Radicais Livres/análise , beta Caroteno/análise , FMN Redutase/análise , Cromatografia Gasosa-Espectrometria de Massas
2.
An Acad Bras Cienc ; 90(1): 449-459, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29424393

RESUMO

This study evaluated the chemical composition and antioxidant activity of fatty acids from the marine red algae Pterocladiella capillacea (S. G. Gmelin) Santelices & Hommersand 1997 and Osmundaria obtusiloba (C. Agardh) R. E. Norris 1991. The gas chromatography mass spectrometry (GC-MS) identified nine fatty acids in the two species. The major fatty acids of P. capillacea and O. obtusiloba were palmitic acid, oleic acid, arachidonic acid and eicosapentaenoic acid. The DPPH radical scavenging capacity of fatty acids was moderate ranging from 25.90% to 29.97%. Fatty acids from P. capillacea (31.18%) had a moderate ferrous ions chelating activity (FIC), while in O. obtusiloba (17.17%), was weak. The ferric reducing antioxidant power (FRAP) of fatty acids from P. capillacea and O. obtusiloba was low. As for ß-carotene bleaching (BCB), P. capillacea and O. obtusiloba showed a good activity. This is the first report of the antioxidant activities of fatty acids from the marine red algae P. capillacea and O. obtusiloba.


Assuntos
Antioxidantes/análise , Antioxidantes/química , Ácidos Graxos/análise , Ácidos Graxos/química , Rodófitas/química , Análise de Variância , FMN Redutase/análise , Sequestradores de Radicais Livres/análise , Cromatografia Gasosa-Espectrometria de Massas , Valores de Referência , Fatores de Tempo , beta Caroteno/análise
3.
An Acad Bras Cienc ; 87(2): 1113-23, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25860969

RESUMO

Marine invertebrates are capable of synthesizing bioactive compounds, which may be beneficial to human health. The aim of this study was to evaluate the antioxidant, hemolytic, antimicrobial and cytotoxic activities of crude extract (70% EtOH), and dichloromethane (DCM), ethyl acetate (EtOAc), and aqueous (Aq) fractions of the marine zoanthid Palythoa caribaeorum. The phenolic compound contents of the crude extract, DCM, EtOAc and Aq fractions were 12.33, 18.17, 10.53, and 3.18 mg GAE per gram, respectively. DPPH radical scavenging activity showed slight variation. IC50 of crude extract, DCM, EtOAc and Aq fractions were 11.13, 11.25, 11.74, and 11.28 µg mL(-1), respectively. Among the sample, ferrous ion chelating was the highest in crude extract (IC50 302.90 µg mL(-1)), followed by EtOAc, Aq, and DCM fractions with 457.77, 547.91, and 641.82 µg mL(-1), respectively. Ferric-reducing antioxidant power showed optical density at about 0.5. The samples tested exhibited low hemolytic activity under 10% up to a concentration of 50 µg mL(-1). No antimicrobial activity was observed against any of the tested bacterial strains. For the cytotoxic activity, LC50 of DCM, crude extract, EtOAc, and Aq were 52.10, 83.06, 86.34, and 117.45 µg mL(-1), showing high toxicity.


Assuntos
Antozoários/química , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Artemia/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Animais , Antibacterianos/isolamento & purificação , Antioxidantes/isolamento & purificação , Bioensaio , Relação Dose-Resposta a Droga , Concentração Inibidora 50 , Testes de Sensibilidade Microbiana
4.
An Acad Bras Cienc ; 86(1): 251-63, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24676166

RESUMO

Natural antioxidants found in marine macroalgae are bioactive compounds known to play an important role in the prevention of diseases associated with aging cells protecting them against the oxidative damage. The purpose of this study was to evaluate the antioxidant and cytotoxic activity of ethanolic extracts of two species of red seaweeds, Amansia multifida and Meristiella echinocarpa. In vitro antioxidant activity was determined by DPPH radical scavenging assay, ferric-reducing antioxidant power (FRAP) assay, ferrous ion chelating (FIC) assay, ß-carotene bleaching (BCB) assay and total phenolic content (TPC) quantification. Cytotoxicity was evaluated with the brine shrimp Artemia sp. lethality test. The TPC values observed in the present study indicated that both species A. multifida and M. echinocarpa are rich in phenolic compounds, reaching values of 45.40 and 28.46 mg gallic acid equivalent (GAE) g-1 of ethanolic extract, respectively. DPPH radical scavenging and ferrous ion chelating showed values of 60% and 17%, respectively. Both seaweed extracts inhibited ß-carotene oxidation by approximately 40%. None of the algal extracts were potentially cytotoxic. The results have showed that extracts of both species of marine red algae exhibit antioxidant potential and low toxicity. They are sources of natural antioxidant compounds.


Assuntos
Antioxidantes/farmacologia , Alga Marinha/química , Animais , Antioxidantes/toxicidade , Artemia/efeitos dos fármacos , Bioensaio , Brasil , Oxirredução
5.
Chem Biol Interact ; 183(3): 369-79, 2010 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-19962971

RESUMO

(+)-Cordiaquinone J is a 1,4-naphthoquinone isolated from the roots of Cordia leucocephala that has antifungal and larvicidal effects. However, the cytotoxic effects of (+)-cordiaquinone J have never being explored. In the present study, the effect of (+)-cordiaquinone J on tumor cells viability was investigated, showing IC(50) values in the range of 2.7-6.6muM in HL-60 and SF-295 cells, respectively. Studies performed in HL-60 leukemia cells indicated that (+)-cordiaquinone J (1.5 and 3.0muM) reduces cell viability and 5-bromo-2-deoxyuridine incorporation after 24h of incubation. (+)-Cordiaquinone J showed rapid induction of apoptosis, as indicated by phosphatidylserine externalization, caspase activation, DNA fragmentation, morphologic changes, and rapid induction of necrosis, as indicated by the loss of membrane integrity and morphologic changes. (+)-Cordiaquinone J altered the redox potential of cells by inducing the depletion of reduced GSH intracellular content, the generation of reactive oxygen species and the loss of mitochondrial membrane potential. However, pre-treatment of cells with N-acetyl-l-cysteine abolished most of the observed effects related to (+)-cordiaquinone J treatment, including those involving apoptosis and necrosis induction.


Assuntos
Apoptose , Leucemia/tratamento farmacológico , Mitocôndrias/efeitos dos fármacos , Naftoquinonas/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Caspase 3/metabolismo , Caspase 7/metabolismo , Linhagem Celular Tumoral , Cordia/química , Glutationa/metabolismo , Células HL-60 , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Mitocôndrias/metabolismo , Naftoquinonas/isolamento & purificação , Naftoquinonas/uso terapêutico , Necrose , Raízes de Plantas/química , Espécies Reativas de Oxigênio/metabolismo
6.
Chem Biodivers ; 6(8): 1224-31, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19697341

RESUMO

Croton regelianus Muell. Arg., popularly known as 'velame-de-cheiro', is a native plant from the Northeast of Brazil used in folk medicine to treat diseases of different kinds, including malignant tumors. In this study, the in vitro and in vivo antitumor effects of the essential oil from the leaves of C. regelianus and ascaridole, one of the main constituents, were investigated. In vitro, the essential oil and ascaridole displayed cytotoxicity, showing IC(50) values in the range of 22.2 to 48.0 microg/ml in HL-60 and SF-295 cell lines for the essential oil, and 6.3 to 18.4 microg/ml in HL-60 and HCT-8 cells lines for ascaridole, respectively. The in vivo study, using sarcoma 180 as a tumor model, demonstrated inhibition rates of 28.1 and 31.8% for essential oil, at the 50 and 100 mg/kg, while ascaridole inhibition rates were 33.9% at 10 mg/kg and 33.3% at 20-mg/kg doses. Histopathological examination showed that the organs were only weakly affected by the treatment. In conclusion, ascaridole has an interesting antitumor activity in sarcoma 180 murine model, probably related to the described cytotoxic activity, and, moreover, its presence in the essential oil from the leaves of C. regelianus could explain, at least in part, the ethnopharmacological use of this plant in the treatment of cancer.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Monoterpenos/farmacologia , Óleos Voláteis/farmacologia , Peróxidos/farmacologia , Óleos de Plantas/farmacologia , Animais , Linhagem Celular Tumoral , Croton/química , Monoterpenos Cicloexânicos , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Camundongos , Folhas de Planta/química , Sarcoma 180
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