RESUMO
Three new polyhydroxylated spirostanol steroidal saponins, dulongenosides B-D (2-4), along with 14 known compounds, dulongenoside A (1), padelaoside B (5), parisyunnanoside G (6), polyphyllin D (7), ophiopogonin C' (8), formosanin C (9), dioscin (10), paris saponin VII (11), paris H (12), parisyunnanoside I (13), protodioscin (14), proprotogracillin (15), crustecdysone (16), and stigmasterol-3-O-ß-d-glucopyranoside (17), were isolated from the rhizomes of Paris dulongensis (Melanthiaceae). Their chemical structures were elucidated based on extensive analyses of NMR and MS data and acidic hydrolyses. The isolates were evaluated for their cytotoxicity to five human cancer cell lines (HL-60, SW480, MDA-MB-231, A549, and A549/Taxol) and the normal human bronchial epithelial cell line BEAS-2B by the MTS test. Compounds 7-12 and 14 showed cytotoxic activity, with IC50 values ranging from 0.20 to 4.35â µM. Proprotogracillin selectively inhibited A549 (IC50=0.58â µM) and A549/Taxol (IC50=0.74â µM) cells, with no significant cytotoxic activity against HL-60, SW480, MDA-MB-231, or BEAS-2B cells, with IC50 values greater than 40â µM.
Assuntos
Antineoplásicos Fitogênicos , Ensaios de Seleção de Medicamentos Antitumorais , Melanthiaceae , Rizoma , Saponinas , Espirostanos , Humanos , Saponinas/isolamento & purificação , Saponinas/farmacologia , Saponinas/química , Rizoma/química , Melanthiaceae/química , Espirostanos/química , Espirostanos/isolamento & purificação , Espirostanos/farmacologia , Linhagem Celular Tumoral , Antineoplásicos Fitogênicos/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Proliferação de Células/efeitos dos fármacos , Relação Estrutura-Atividade , Sobrevivência Celular/efeitos dos fármacos , Estrutura Molecular , Conformação Molecular , Relação Dose-Resposta a DrogaRESUMO
Five new steroidal saponins, paripolins D-H (1-5), and 6 known compounds (6-11) were isolated from the aerial parts of Paris polyphylla var. yunnanensis. The structures of 1-5 were determined using spectroscopic analyses in conjunction with acid hydrolysis. It is for the first time to report the 12-hydroxysteroidal saponins from the genus Paris. The effect of all isolated compounds on blood coagulation was determined in vitro using the plasma recalcification time method. Compounds 1 and 2 showed potent procoagulant activity, and 5-11 exhibited significant anticoagulant activity.
Assuntos
Liliaceae , Saponinas , Liliaceae/química , Rizoma/química , Estrutura Molecular , Saponinas/farmacologia , Saponinas/química , Coagulação SanguíneaRESUMO
Phytochemical investigation of an extract of the aerial parts of Paris polyphylla var. yunnanensis resulted in the identification of three new steroidal sapogenins, namely as paripolins A-C (1-3). With the aid of comprehensive spectroscopic techniques (NMR, IR, UV, MS), the structures of all isolated compounds were elucidated and subsequently screened for anti-inflammatory activity.
Assuntos
Ascomicetos , Liliaceae , Melanthiaceae , Sapogeninas , Saponinas , Saponinas/química , Estrutura Molecular , Esteroides , Liliaceae/química , Componentes Aéreos da PlantaRESUMO
OBJECTIVE: To establish pharmacognostical methods on Root of Geranium strictipes. METHODS: To study by the original plant identification, character identification. The organizational structure of root and powder features of this drug were observed and compared. TLC of the drug was also undertaken. RESULTS: It showed that Geranium strictipes powder microstructure contained many calcium oxalate cluster crystals and starch grains. TLC had good reappearance. CONCLUSION: This study provides reference information for further development and identification of this crude drug.