RESUMO
During robot-assisted rehabilitation, failure to recognize lower limb movement may efficiently limit the development of exoskeleton robots, especially for individuals with knee pathology. A major challenge encountered with surface electromyography (sEMG) signals generated by lower limb movements is variability between subjects, such as motion patterns and muscle structure. To this end, this paper proposes an sEMG-based lower limb motion recognition using an improved support vector machine (SVM). Firstly, non-negative matrix factorization (NMF) is leveraged to analyze muscle synergy for multi-channel sEMG signals. Secondly, the multi-nonlinear sEMG features are extracted, which reflect the complexity of muscle status change during various lower limb movements. The Fisher discriminant function method is utilized to perform feature selection and reduce feature dimension. Then, a hybrid genetic algorithm-particle swarm optimization (GA-PSO) method is leveraged to determine the best parameters for SVM. Finally, the experiments are carried out to distinguish 11 healthy and 11 knee pathological subjects by performing three different lower limb movements. Results demonstrate the effectiveness and feasibility of the proposed approach in three different lower limb movements with an average accuracy of 96.03% in healthy subjects and 93.65% in knee pathological subjects, respectively.
Assuntos
Algoritmos , Eletromiografia , Extremidade Inferior , Movimento , Máquina de Vetores de Suporte , Humanos , Eletromiografia/métodos , Extremidade Inferior/fisiologia , Masculino , Adulto , Movimento/fisiologia , Feminino , Processamento de Sinais Assistido por Computador , Adulto Jovem , Músculo Esquelético/fisiologiaRESUMO
Drought stress significantly impacts global rice production, highlighting the critical need to understand the genetic basis of drought resistance in rice. Here, through a genome-wide association study, we reveal that natural variations in DROUGHT RESISTANCE GENE 9 (DRG9), encoding a double-stranded RNA (dsRNA) binding protein, contribute to drought resistance. Under drought stress, DRG9 condenses into stress granules (SGs) through liquid-liquid phase separation via a crucial α-helix. DRG9 recruits the mRNAs of OsNCED4, a key gene for the biosynthesis of abscisic acid, into SGs and protects them from degradation. In drought-resistant DRG9 allele, natural variations in the coding region, causing an amino acid substitution (G267F) within the zinc finger domain, increase DRG9's binding ability to OsNCED4 mRNA and enhance drought resistance. Introgression of the drought-resistant DRG9 allele into the elite rice Huanghuazhan significantly improves its drought resistance. Thus, our study underscores the role of a dsRNA-binding protein in drought resistance and its promising value in breeding drought-resistant rice.
Assuntos
Resistência à Seca , Oryza , Oryza/genética , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Estudo de Associação Genômica Ampla , Separação de Fases , Estresse Fisiológico/genética , Melhoramento Vegetal , Secas , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo , Regulação da Expressão Gênica de PlantasRESUMO
Flash droughts have gained considerable public attention due to the imminent threats they pose to food security, ecological safety, and human health. Currently, there has been little research exploring the projected changes in flash droughts and their association with compound meteorological extremes (CMEs). In this study, we applied the pentad-mean water deficit index to investigate the characteristics of flash droughts and their association with CMEs based on observational data and downscaled model simulations. Our analysis reveals an increasing trend in flash drought frequency in China based on historical observations and model simulations. Specifically, the proportion of flash drought frequency with a one-pentad onset time showed a consistent upward trend, with the southern parts of China experiencing a high average proportion during the historical period. Furthermore, the onset dates of the first (last) flash droughts during year are projected to shift earlier (later) in a warmer world. Flash droughts become significantly more frequent in the future, with a growth rate approximately 1.3 times higher in the high emission scenario than in the medium emission scenario. The frequency of flash droughts with a one-pentad onset time also exhibits a significant upward trend, indicating that flash droughts will occur more rapidly in the future. CMEs in southern regions of China were found to be more likely to trigger flash droughts in the historical period. The probability of CMEs triggering flash droughts is expected to increase with the magnitude of warming, particularly in the far-future under the high emissions scenario.