A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3KLHL22.

The CUL3-RING E3 ubiquitin ligases (CRL3s) play an essential role in response to extracellular nutrition and stress stimuli. The ubiquitin ligase function of CRL3s is activated through dimerization. However, how and why such a dimeric assembly is required for its ligase activity remains elusive. Here, we report the cryo-EM structure of the dimeric CRL3KLHL22 complex and reveal a conserved N-terminal motif in CUL3 that contributes to the dimerization assembly and the E3 ligase activity of CRL3KLHL22. We show that deletion of the CUL3 N-terminal motif impairs dimeric assembly and the E3 ligase activity of both CRL3KLHL22 and several other CRL3s. In addition, we found that the dynamics of dimeric assembly of CRL3KLHL22 generates a variable ubiquitination zone, potentially facilitating substrate recognition and ubiquitination. These findings demonstrate that a CUL3 N-terminal motif participates in the assembly process and provide insights into the assembly and activation of CRL3s.

Drug delivery system based on metal-organic framework improved 5-Fluorouracil against spring viremia of carp virus.

Spring viremia of carp virus (SVCV), as a high pathogenicity pathogen, has seriously restricts the healthy and sustainable development of cyprinid farming industry. In this study, we selected 5-Fluorouracil (5-Fu) as the drug model based on zeolitic imidazolate framework-8 (ZIF-8) to construct a drug delivery system (5-Fu@ZIF-8), and the anti-SVCV activity was detected in vitro and in vivo. The results showed 5-Fu@ZIF-8 was uniform cubic particle with truncated angle and smooth surface, and the particle size was 90 nm. The anti-SVCV activity in vitro results showed that the highest inhibition rate of 5-Fu was 77.93% at 40 mg/L and the inhibitory concentration at half-maximal activity (IC50) was 20.86 mg/L. For 5-Fu@ZIF-8, the highest inhibition rate was 91.36% at 16 mg/L, and the IC50 value was 5.85 mg/L. In addition, the cell viability was increased by 18.1% after 5-Fu treatment. Similarly, after 5-Fu@ZIF-8 treatment, the cell viability increased by 27.3%. Correspondingly, in vivo experimental results showed the viral loads reduced by 18.1% on the days 7 and the survival rate increased to 19.4% at 80 mg/L after 5-Fu treatment. For 5-Fu@ZIF-8, the viral loads reduced by 41.2% and the survival rate increased to 54.8%. Mechanistically, 5-Fu inhibits viral replication by regulating p53 expression and promoting early apoptosis in infected cells. All results indicated that 5-Fu@ZIF-8 improved the anti-SVCV activity; it may be a potential strategy to construct a drug-loaded system with ZIF-8 as a carrier for the prevention and treatment of aquatic diseases.

Biogenic elements-informed assessment of the impact of human activities on river ecosystems.

River ecosystems, acting as pivotal conduits linking terrestrial, marine, and atmospheric realms, have faced significant disturbances due to human exploitation of their resources. Recent years have witnessed a heightened intensification of human activities, adversely affecting the equilibrium of water ecosystems. To systematically study the various factors that affect river ecosystems under human activities, we introduce a universally applicable approach that considers the diversity of watersheds, biogenic elements, and human activities. Using this method, this application uncovers the sensitive human activity types, biogenic factors, and species significantly influencing river biodiversity within the study area. Incorporating statistical modelling, sensitivity screening, and advanced correlation analyses within a random forest regression framework, Sensitive biogenic elements and biological types affected by human activities were identified in typical watersheds, and the stability of different aquatic ecosystems was evaluated. Suggestions for watershed management measures were proposed When human activities affect the degree of water resource development and utilization, the forms of sensitive biogenic elements include DIC and Tsi; When human activities affect the discharge of pollutants into rivers, the forms of sensitive biogenic elements include TP, PP, and DEP, and the ratio composition includes TC: TN, TC: TP, TP: TSi, and TN: TP, This study pioneers a novel method for assessing human impacts on river ecosystems and successfully applies this approach to inform management decisions for river segments and tributaries in the middle and upper reaches of the Yangtze River basin. thereby enhancing our understanding of the consequences of human-induced impacts on biodiversity.

Structural insights into the ubiquitylation strategy of the oligomeric CRL2FEM1B E3 ubiquitin ligase.

Cullin-RING E3 ubiquitin ligase (CRL) family members play critical roles in numerous biological processes and diseases including cancer and Alzheimer's disease. Oligomerization of CRLs has been reported to be crucial for the regulation of their activities. However, the structural basis for its regulation and mechanism of its oligomerization are not fully known. Here, we present cryo-EM structures of oligomeric CRL2FEM1B in its unneddylated state, neddylated state in complex with BEX2 as well as neddylated state in complex with FNIP1/FLCN. These structures reveal that asymmetric dimerization of N8-CRL2FEM1B is critical for the ubiquitylation of BEX2 while FNIP1/FLCN is ubiquitylated by monomeric CRL2FEM1B. Our data present an example of the asymmetric homo-dimerization of CRL. Taken together, this study sheds light on the ubiquitylation strategy of oligomeric CRL2FEM1B according to substrates with different scales.

Structural insights into the conformational changes of BTR1/SLC4A11 in complex with PIP2.

BTR1 (SLC4A11) is a NH3 stimulated H+ (OH-) transporter belonging to the SLC4 family. Dysfunction of BTR1 leads to diseases such as congenital hereditary endothelial dystrophy (CHED) and Fuchs endothelial corneal dystrophy (FECD). However, the mechanistic basis of BTR1 activation by alkaline pH, transport activity regulation and pathogenic mutations remains elusive. Here, we present cryo-EM structures of human BTR1 in the outward-facing state in complex with its activating ligands PIP2 and the inward-facing state with the pathogenic R125H mutation. We reveal that PIP2 binds at the interface between the transmembrane domain and the N-terminal cytosolic domain of BTR1. Disruption of either the PIP2 binding site or protonation of PIP2 phosphate groups by acidic pH can transform BTR1 into an inward-facing conformation. Our results provide insights into the mechanisms of how the transport activity and conformation changes of BTR1 are regulated by PIP2 binding and interaction of TMD and NTD.

Engineering of Stable Anionic/Neutral MOFs with Zinc-Adeninate Building Units for Efficient C2H2/CO2 Separation.

Using adenine and metal ions to form secondary building units (SBUs), further connected by a highly symmetrical multicarboxylic linker to construct an amino-modified porous framework with high porosity, is an effective strategy. By regulating the deprotonation and hydrolysis capacity of the synthesized solvent, it is possible to obtain different charged frameworks. In this work, two stable anionic/neutral MOFs, (Et2NH2)[Zn3(TCPE)(adenine)2CH3COO]·DEF·3H2O (1) and [Zn3.5(adenine)(TCPE)1.5(DMA)(H2O)0.5]·2DMA·2H2O (2), have been synthesized based on zinc-adeninate building units and symmetric tetrakis(4-carboxyphenyl)ethylene (H4TCPE) in N,N-diethylformamide (DEF) and N,N-dimethylacetamide (DMA) reaction systems, respectively. 1 is an anionic framework based on 1D rod zinc-adeninate SBU, containing 1D rectangular (14.3 × 6.3 Å2) and square (14.3 × 14.3 Å2) channels. While 2 is a neutral framework built from isolated zinc-adeninate SBU, it contains hexagonal cages with a dimension of 5.5 Å in the structure. Both of them have high porosity (61.6% for 1 and 46.3% for 2) and high stability in a wide range of pH. 1 and 2 show high C2H2 adsorption capacity at 298 K (48.1 and 70.1 cm3 g-1, respectively) and selective capacity for C2H2/CO2 mixtures, which was confirmed by the breakthrough experiments. Furthermore, the interaction between the frameworks and gas molecules has also been explained by theoretical calculation. This work provides a good example of the design and regulation of porous structures for adsorption and separation functions.

Aromatic Terpenes and Their Biosynthesis in Dendrobium, and Conjecture on the Botanical Perfumer Mechanism.

This review presents a systematic analysis of the studies on volatiles in Dendrobium. Among the various components, aromatic terpenes are a crucial component in the development of the aromatic characteristics of Dendrobium and other plants. Recent advancements in detection and sequencing technology have resulted in a considerable rise in research on the biosynthetic processes of aromatic terpenes in Dendrobium and other flowering plants. Nevertheless, the inquiry into the precise means by which plants regulate the proportion of diverse aromatic terpenes in their floral scent, thereby preserving their olfactory traits, requires further investigation. A conjecture on the botanical perfumer mechanism, which condensed the findings of earlier studies, was put forward to address this area of interest. Specific transcription factors likely govern the coordinated expression of multiple key terpene synthase (TPS) genes during the flowering stage of plants, thereby regulating the proportional biosynthesis of diverse aromatic terpenes and sustaining the distinctive aromatic properties of individual plants. This review serves as a significant theoretical reference for further investigations into aromatic volatile compounds in Dendrobium.

Sociocultural influences on alcohol expectancies in early adolescence: Findings from the ABCD study.

OBJECTIVE: Alcohol expectancies (AE) during early adolescence predict early alcohol use initiation and problem drinking both cross-sectionally and prospectively well into adulthood. Yet, our understanding of the sociocultural factors associated with AE during this development period remains limited. This study examines associations between AE and sociocultural factors across various domains (i.e., individual, family, peer, school, community, and culture) in a demographically diverse sample of 10- to 14-year-old youth in the Adolescent Brain Cognitive Development Study (ABCD Study). METHOD: This cross-sectional study used 2-year follow-up data from the ABCD Release 3.0 for N = 5,322 early adolescents (Mage = 12 years [SD = 0.6]; 47% male). Approximately 60% identified as non-Hispanic/Latinx White, 17% as Hispanic/Latinx, 11% as non-Hispanic/Latinx Black, 2% as non-Hispanic/Latinx Asian, and 11% as mixed/other race-ethnicity. Separate models for positive and negative AE outcomes were conducted using linear mixed-effect models while controlling for demographic covariates. RESULTS: Positive AE were most strongly associated with familism, followed by other peer, school, community, and cultural level factors. Negative AE were most strongly associated with the peer-level factor of relational victimization and the individual-level factor of negative life events, followed by other peer, school, and community-level factors. CONCLUSION: The present findings reveal the potential constellation of sociocultural factors that may serve as targets for modifying AE during the middle school years. Study results also underscore the need for future research that integrates cultural factors into our understanding of alcohol use risk and resilience during early adolescence. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Discriminatory Gate-Opening Effect in a Flexible Metal-Organic Framework for Inverse CO2 /C2 H2 Separation.

Considering the significant application of acetylene (C2 H2 ) in the manufacturing and petrochemical industries, the selective capture of impurity carbon dioxide (CO2 ) is a crucial task and an enduring challenge. Here, a flexible metal-organic framework (Zn-DPNA) accompanied by a conformation change of the Me2 NH2 + ions in the framework is reported. The solvate-free framework provides a stepped adsorption isotherm and large hysteresis for C2 H2 , but type-I adsorption for CO2 . Owing to their uptakes difference before gate-opening pressure, Zn-DPNA demonstrated favorable inverse CO2 /C2 H2 separation. According to molecular simulation, the higher adsorption enthalpy of CO2 (43.1 kJ mol-1 ) is due to strong electrostatic interactions with Me2 NH2 + ions, which lock the hydrogen-bond network and narrow pores. Furthermore, the density contours and electrostatic potential verifies the middle of the cage in the large pore favors C2 H2 and repels CO2 , leading to the expansion of the narrow pore and further diffusion of C2 H2 . These results provide a new strategy that optimizes the desired dynamic behavior for one-step purification of C2 H2 .

Dative B←N bonds based crystalline organic framework with permanent porosity for acetylene storage and separation.

The utilization of dative B←N bonds for the creation of crystalline organic framework (BNOF) has increasingly received intensive interest; however, the shortage of permanent porosity is an obstacle that must be overcome to guarantee their application as porous materials. Here, we report the first microporous crystalline framework, BNOF-1, that is assembled through sole monomers, which can be scalably synthesized by the cheap 4-pyridine boronic acid. The 2D networks of BNOF-1 were stacked in parallel to generate a highly porous supramolecular open framework, which possessed not only the highest BET surface area of 1345 m2 g-1 amongst all of the BNOFs but also features a record-high uptake of C2H2 and CO2 in covalent organic framework (COF) materials to date. Dynamic breakthrough experiments demonstrated that BNOF-1 material can efficiently separate C2H2/CO2 mixtures. In addition, the network can be regenerated in organic solvents with no loss in performance, making its solution processable. We believe that BNOF-1 would greatly diversify the reticular chemistry and open new avenues for the application of BNOFs.

Alcohol use severity among recent Latino immigrants: Associations of acculturation, family history of alcohol use and alcohol outcome expectancies.

Having a family history of alcohol (FH+) use is a well-documented risk factor for alcohol use and alcohol related problems. However, there are limited studies examining the impact of FH + on current alcohol use among Latino immigrants. This study aimed to determine the influence of having a FH + on current alcohol use among Latino immigrants and the influence of alcohol outcome expectancies (AOEs) and acculturation on this relationship. This is a longitudinal secondary data analysis of data from the Recent Latino Immigrant Study (RLIS), the first community-based cohort study to examine pre- to post-immigration alcohol use trajectories of young adult Latino immigrants. Linear mixed models were performed to assess the association between various pre- and post-immigration factors and alcohol use among Latino immigrants. There were 518 young adult Latino immigrants with 18.7% reporting a FH + with those with a FH + having higher mean AUDIT score compared to those without (4.74 vs. 3.81; p = 0.028). Positive AOEs were associated with increase AUDIT scores. FH + individuals with greater positive AOEs experienced higher AUDIT scores compared to FH- individuals. Family cohesion was protective against alcohol use while endorsement of Americansism was associated with increased alcohol use. Theses results provide the framework for more in-depth exploration regarding the influences of FH+, AOEs, and acculturation have on the alcohol use among Latino immigrants. Future longitudinal research studies should account for whether traditional cultural values mediate or moderate the relationship between a FH+, AOE, and alcohol use of Latino immigrants.

Predictors of Alcohol Use Among Latinx Men in South Florida: Machismo as a Correlate of Alcohol Use Frequency and Quantity.

Previous studies have found Latinx cultural values to be positively associated with healthy behaviors. This study aims to examine socioeconomic and cultural correlates of alcohol use among Latinx adult men living in Miami-Dade County, Florida. The study sample included 122 Latinx adult men (mean age = 44, SD = 10), predominantly of South and Central American origin. Data was collected using REDCap. Interviews included the Timeline Follow-Back scale for alcohol use. Results indicate that Caribbean participants were significantly less likely to report drinking in the past 90 days (aOR = 0.08, p = 0.042) compared to their Venezuelan counterparts. Higher machismo scores were associated with low drinking frequency (aRR = 0.67, p = 0.043), while no significant associations were found between machismo and other drinking outcomes. Drinking quantity and frequency are significantly associated with higher income and authorized immigration status in the US among Latinx men in South Florida. Higher machismo scores were associated with low drinking frequency.

Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum.

Dendrobium chrysotoxum is considered as an important ornamental dendrobium because of its strong and long-lasting floral scent. Nevertheless, few information is known about the dynamic changes and related formation mechanism of dendrobium floral scent at different flowering stages. In this study, the characteristics and biosynthetic mechanism of floral scent in D. chrysotoxum during flowering was revealed by using widely-targeted volatilomics (WTV) combined with transcriptome analysis. Over 500 kinds of volatile organic compounds (VOCs) were detected in the floral scents of D. chrysotoxum, which improved the knowledge about floral scent components of dendrobium. A total of 153 differential VOCs and 4,487 differentially expressed genes (DEGs) were identified between flowers of different flowering stages, respectively. The results for both volatilomics and transcriptomics data indicated that terpenes and related genes played an important role in the formation of floral characteristics of D. chrysotoxum. But in general, the expression of genes showed an opposite trend to the accumulation of metabolites during flowering, suggesting that the regulation of floral scent biosynthesis might have started at the budding stage in D. chrysotoxum. Additionally, a transcriptional metabolic regulatory network consisting of terpenes, terpene synthases and candidate transcription factors was established. This research is the first systematic and comprehensive exploration of floral characteristics and related mechanisms during flowering in D. chrysotoxum. It provides basis for exploration of mechanisms on the floral scents and the breeding of aromatic dendrobium.

Rational Synthesis of a Stable Rod MOF for Ultrasensitive Detection of Nitenpyram and Nitrofurazone in Natural Water Systems.

Overuse of nitenpyram and nitrofurazone in agricultural products poses enormous risks to ecosystems, and effective detection and quantification of these residual pollutants are of great concern. Although several strategies have been established for detecting nitenpyram and nitrofurazone in water, searching for a new sensor material with great sensitivity, selectivity, and recyclability remains challenging. Here, we design and synthesize a stable metal-organic framework (MOF) (Zn-CPTA) by employing an organic linker based on the coordination features of benzene-1,4-dicarboxylate and picolinic acid. Zn-CPTA is a 3D framework built from Zn-O-Zn chains called rod secondary building units, which contains 1D open channels modified by uncoordinated carboxyl O atoms and exhibits impressive chemical stability in aqueous solutions within a pH range from 2 to 12. Especially, fluorescent Zn-CPTA can quickly and sensitively detect nitenpyram and nitrofurazone in aqueous solutions with a high quenching constant and low detection limit (LOD) (KSV values for nitenpyram and nitrofurazone are 1.67 × 104 and 1.02 × 105 M-1 with LOD of 0.625 and 0.126 µM, respectively), as well as outstanding selectivity and recyclability. Notably, the LOD value is the lowest among the reported MOFs used for nitrofurazone detection. Besides, experiments and density functional theory calculations are combined to explain the quenching mechanism. Finally, the practical application of Zn-CPTA was further explored in real environment samples with satisfactory recoveries.

Hexagonal Cages and Lewis Acid-Base Sites in a Metal-Organic Framework for Synergistic CO2 Capture and Conversion under Mild Conditions.

The cycloaddition reaction of carbon dioxide (CO2) is a highly economic solution to becoming carbon-neutral. Herein, we have designed and synthesized a robust zinc(II)-organic framework (Zn-Ade-TCPE) by a function-directed strategy. Zn-Ade-TCPE possesses uncommon hexagonal cages with Lewis acid-base bifunctional sites and displays a high adsorption capacity for CO2. At room temperature and atmospheric pressure, Zn-Ade-TCPE exhibits outstanding activity, selectivity, and recyclability in the cycloaddition reaction of epoxides with CO2 because of the synergistic effect of multiple active sites and confined cavities.

Quantitative Chemoproteomic Profiling of Protein Cross-Links Induced by Methylglyoxal.

Methylglyoxal (MGO) is a highly reactive metabolite mainly formed as a byproduct of glycolysis. Elevated MGO has been considered as a risk factor for several diseases including diabetes and neurodegeneration. While MGO modifications on proteins were globally profiled, the cross-links between proteins induced by MGO in proteomes are unexplored to date. Here, we reported a quantitative chemoproteomic platform based on mass shifts that enables identification of events of protein cross-links induced by MGO in proteomes. A total of 66 cross-linked targets were identified from the profiling experiments when cells were treated with MGO, among which the components of functional complexes such as spliceosomes and ribosomes were enriched. We found that inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) was homocross-linked by MGO and the active-site Cys331 was critical for mediating the cross-link, which in turn affected IMPDH2's activity. Our study has provided new clues for the functional impact in proteomes by MGO, and the methodology can be, in principle, applied to profile protein cross-links induced by other reactive metabolites.

Effects of Corneal Stromal Lens Collagen Cross-Linking Regraft on Corneal Biomechanics.

Background: Corneal collagen cross-linking (CXL) therapy, a method that uses a combination of riboflavin and ultraviolet-A light (UVA), can promote the formation of covalent cross-linking of amino acid residues of corneal collagen and enhance the hardness of the cornea. In this study, we explored the effects of corneal stromal lens collagen cross-linking regraft on corneal biomechanics. Methods: A total of 15 New Zealand white rabbits were divided into 3 groups: normal control group (group A), SMILE + uncross-linked lens implantation group (Group B), and SMILE + cross-linking lens implantation group (group C). The design parameters of SMILE surgery were as follows: the corneal cap was 120 um thick, the lens diameter was 6.5 mm, and the diopter was -6.0D. Riboflavin and ultraviolet-A (UVA) were used as corneal stromal lens CXL, which was implanted into the allogeneic rabbit corneal stromal bag 24 hours after the operation. Postoperative corneal thickness (CCT), refraction, AS-OCT, and corneal biomechanics were performed before and then at 1 and 3 months after the operation. Results: All corneas appeared transparent and smooth 3 months after surgery. The corneal thicknesses of both group B and group C were lower than those before the operation. The corrected refraction of group B and group C after lens implantation was also lower than the expected corrected power; there was no significant difference between the two groups (P > 0.05). AS-OCT results showed an uneven surface and thickness of the corneal stromal lens in two eyes of group B. Moreover, corneal elastic deformation increased with intraocular pressure in each group; displacement from large to small was group B > group C and > group A. The creep from large to small was group B > group C > group A. The fiberboard layers of groups B and C were disordered, and there were a few autophagosomes in the fibroblasts of group B by transmission electron microscopy (TEM). Conclusions: Allograft graft of corneal stromal lens collagen cross-linked can significantly increase the biomechanical properties of the cornea.

Pre- and post-immigration factors associated with cigarette use among young adult recent Latinx immigrants during their initial year in the U.S.

Background: Evidence indicates escalating rates of cigarette use among foreign-born Latinx as their time in the U.S increases. As such, it's important to understand shifts in pre- to post-immigration smoking patterns early in the immigration process and its associated factors.Objectives: To examine 1) cigarette use among recent Latinx immigrants (RLIs) during their initial year in the U.S.; 2) whether cigarette use after immigration is influenced by smoking patterns in immigrant's country of origin; and 3) associations between pre/post immigration sociocultural factors and changes in cigarette use after immigration.Methods: Baseline data were utilized from an on-going longitudinal study of 540 young adult (50% females) RLIs. Inclusion criteria was being between ages 18 and 34, residing in Miami-Dade County, Florida, and having immigrated from a Latin American country within the past yearResults: Approximately 31% of participants reported being smokers in their country of origin while 26% were current smokers (while residing in the U.S). Post-immigration cigarette use was substantially influenced by country of origin cigarette use (V = .68); 84% of pre-immigration smokers reported no change in smoking frequency, while 11% lowered and 6% increased their cigarette use post-immigration. Reduction in smoking after immigration was more likely among participants with higher pre-immigration social support (aOR = 1.87) and less likely among those residing in high-crime neighborhoods (aOR = .84).Conclusion: Interventions aimed to discourage cigarette use should begin early in the immigration process and account for RLIs' pre-immigration smoking patterns. Interpersonal supports and neighborhood contextual factors should be considered when developing smoking cessation programs with this population.

Indium metal-organic frameworks based on pyridylcarboxylate ligands and their potential applications.

Indium metal-organic frameworks (In-MOFs) based on pyridylcarboxylate ligands represent a subclass of MOFs featuring diverse structures, a high stability, and various properties. This review discusses the different aspects of In-MOFs including their design, synthesis and structures as well as their typical potential applications in adsorption and separation, catalysis, and chemical sensors. Importantly, the effect of pyridine on the properties and stability of frameworks has been carefully studied. The introduction of a pyridine group not only significantly enriches clusters of In3+ ions, but also enables flexible, controllably synthesized ionic or neutral frameworks to be fabricated. Based on this, we suggest that this type of In-metal organic framework (MOF) should receive more attention in the field of MOF design.

Scenario analysis for the sustainable development of agricultural water in the Wuyuer River basin based on the WEP model with a reservoir and diversion engineering module.

Agricultural water use is increasing quickly with the rapid socioeconomic development observed in the Wuyuer River basin. Water withdrawal for agriculture over the past decade have seriously depleted the ecological water requirements in the basin and damaged the channel and downstream wetland ecosystems. Achieving sustainable development in the basin will require a balance between agricultural water exploitation and ecological water demands. In this paper, a reservoir and diversion engineering module was integrated with a dualistic distributed hydrological model (WEP model) to investigate the effects of agricultural water use on river discharge. Agricultural water shortages and yearly minimum river discharges between 2020 and 2050 under six agricultural water exploitation scenarios and one natural scenario were estimated based on the proposed model. The results showed that the dualistic hydrological process model was more suitable for basins with agricultural water resource exploitation and that the river discharge was significantly less than the natural discharge due to irrigation and reservoir filling, especially in drought years. Under the scenarios of high, middle and low water resource exploitation without ecological operations, agricultural development was unsustainable because of agricultural water shortages and ecological water scarcity. The evaluation of the guaranteed rates for the agricultural water supply and environmental flows showed that the low water resource exploitation scenario with ecological operations was the best option and that sustainable development could be achieved in the basin under this exploitation scenario in the future. However, implementing water management practices in the basin could result in certain economic losses. These losses could be offset by ecological protection funds for downstream wetlands. Overall, the model results could help to inform planning and investment decisions within the basin to improve the sustainable management of water resources.