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1.
Equine Vet J ; 51(5): 669-673, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30648279

RESUMO

BACKGROUND: Current serological tests cannot discriminate between bactericidal Borrelia burgdorferi antibodies from others that are merely a response to Borrelia antigenic stimulation. OBJECTIVE: To develop a sensitive and convenient luminescence-based serum bactericidal assay (L-SBA) to identify serum borreliacidal activity. STUDY DESIGN: Prospective validation study and method comparison. METHODS: Serum samples were obtained either from archives of the Animal Health Diagnostic Center at Cornell University (N = 7) or from a vaccination trial (N = 238). Endogenous complement-inactivated serum sample was incubated with exogenic complement and B. burgdorferi ML23 pBBE22luc, which is able to process luciferin with luciferase and produce luminescence in viable Borrelia. After incubation, a light signal can be detected by using a luminometer to calculate the borreliacidal antibody titre. RESULTS: Components of the reaction mixture including spirochetes and complement from various sources and concentrations were tested to identify a reliable recipe for our complement-mediated L-SBA. We also applied this L-SBA on measuring bactericidal antibody activities and calculated the half inhibitory concentration (IC50 ) of serum samples from clinical collections. Furthermore, we analysed the L-SBA titres and anti-outer surface protein A (OspA) antibody levels from vaccinated horses using the multiplex assays and found that there is a relationship between results generated using these two different assays. The increases of L-SBA titres correlated with increases of anti-OspA antibody titre in sera (r = 0.423). MAIN LIMITATIONS: Immunoreactivity of commercial complement may differ from different batches. Clinical protection of borreliacidal antibody levels has not been determined. CONCLUSIONS: The L-SBA provided a sensitive and easy-operating platform for the evaluation of bactericidal antibody to B. burgdorferi, and we anticipated L-SBA would function well as an evaluation tool of vaccine efficiency in the future.


Assuntos
Anticorpos Antibacterianos/sangue , Borrelia burgdorferi/imunologia , Doenças dos Cavalos/prevenção & controle , Medições Luminescentes/veterinária , Vacinas contra Doença de Lyme/imunologia , Ensaios de Anticorpos Bactericidas Séricos/veterinária , Animais , Doenças dos Cavalos/sangue , Cavalos , Medições Luminescentes/métodos , Ensaios de Anticorpos Bactericidas Séricos/métodos
2.
Clin Sci (Lond) ; 102(3): 279-86, 2002 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11869168

RESUMO

Platelets and leucocytes are important effector cells of the haemostatic and inflammatory responses to tissue injury. To investigate the effects of surgical trauma on platelet activation (assessed by measuring levels of P-selectin and beta-thromboglobulin), leucocyte activation (CD11b expression) and leucocyte-platelet interactions (leucocyte-platelet complexes), 30 patients undergoing primary hip arthroplasty were studied before and at the end of surgery, and on days 1 and 10 post-operatively, using a whole-blood flow cytometry assay. The inflammatory response was followed by measurement of the levels of C-reactive protein and interleukin-6 in plasma, and the activation of coagulation was monitored by determination of prothrombin fragment 1+2 levels. On day 1 post-operatively a significantly increased expression of CD11b on monocytes was noted, but no direct correlation was found between monocyte activation and interleukin-6 production or C-reactive protein at this time point. The percentage of monocyte-platelet and neutrophil-platelet complexes was markedly increased on day 10 post-operatively compared with pre-operative levels, and levels of these complexes were significantly positively correlated with beta-thromboglobulin levels. Activation of coagulation (prothrombin fragment 1+2) on day 10 post-operatively was positively correlated with the extent of surgical trauma (duration of surgery, amount of blood loss) and with the increase in platelet activation (beta-thromboglobulin). In conclusion, hip arthroplasty induces platelet and coagulation activation, and also an inflammatory response that is maintained for more than 10 days post-operatively. This indicates an interaction between the immune and the haemostatic systems in the post-operative phase after hip arthroplasty.


Assuntos
Artroplastia de Quadril , Plaquetas/fisiologia , Monócitos/fisiologia , Neutrófilos/fisiologia , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Proteína C-Reativa/análise , Adesão Celular , Feminino , Citometria de Fluxo/métodos , Humanos , Interleucina-6/sangue , Ativação Linfocitária , Antígeno de Macrófago 1/sangue , Masculino , Pessoa de Meia-Idade , Selectina-P/sangue , Fragmentos de Peptídeos/análise , Ativação Plaquetária , Período Pós-Operatório , Precursores de Proteínas/análise , Protrombina/análise , Estatísticas não Paramétricas , Trombose Venosa/sangue , beta-Tromboglobulina/análise
3.
Int Orthop ; 25(4): 226-7, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11561496

RESUMO

We performed a prospective randomized study of 74 patients undergoing hip replacement surgery and compared the perioperative blood loss in the lateral position with that in the supine position. The surgeons and surgical technique were the same in the two groups. The patients operated on in the lateral position had a significantly lower total blood loss, on average 201 ml less.


Assuntos
Artroplastia de Quadril/métodos , Perda Sanguínea Cirúrgica/prevenção & controle , Osteoartrite do Quadril/cirurgia , Idoso , Idoso de 80 Anos ou mais , Artroplastia de Quadril/efeitos adversos , Distribuição de Qui-Quadrado , Feminino , Seguimentos , Humanos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Osteoartrite do Quadril/diagnóstico , Postura , Probabilidade , Estudos Prospectivos , Medição de Risco , Decúbito Dorsal , Resultado do Tratamento
6.
Acta Orthop Scand ; 70(3): 268-70, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10429603

RESUMO

We studied the effect of timing of tourniquet release on blood loss in 81 patients (85 knees) who were operated on for total knee replacement. The patients were randomly allocated to one of two groups. In one group, the tourniquet was released for hemostasis before wound closure and in the other group, the tourniquet was not released until the wound was closed and a compressive dressing applied. We found no difference in total blood loss between the two groups and conclude that intraoperative release of the tourniquet for hemostasis is not effective for reducing blood loss in total knee replacement.


Assuntos
Artroplastia do Joelho , Perda Sanguínea Cirúrgica , Hemostasia Cirúrgica/efeitos adversos , Hemostasia Cirúrgica/métodos , Cuidados Intraoperatórios/efeitos adversos , Cuidados Intraoperatórios/métodos , Torniquetes/efeitos adversos , Idoso , Idoso de 80 Anos ou mais , Artrite Reumatoide/cirurgia , Artroplastia do Joelho/efeitos adversos , Bandagens , Perda Sanguínea Cirúrgica/estatística & dados numéricos , Transfusão de Sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Osteoartrite do Joelho/cirurgia , Estudos Prospectivos , Técnicas de Sutura , Fatores de Tempo
7.
Acta Orthop Scand ; 70(1): 23-4, 1999 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10191742

RESUMO

We evaluated bleeding in a prospective randomized study of 67 patients undergoing primary hip arthroplasty. In group A, all dissections were made with the diathermy knife and in group B, we used the scalpel. In both groups, bleeding points were coagulated with diathermy. The surgical approach, use of anti-coagulants and method of anesthesia were similar in the two groups. We found that use of a diathermy knife does not significantly reduce bleeding.


Assuntos
Artroplastia de Quadril/métodos , Perda Sanguínea Cirúrgica/prevenção & controle , Diatermia/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Artroplastia de Quadril/efeitos adversos , Artroplastia de Quadril/instrumentação , Perda Sanguínea Cirúrgica/estatística & dados numéricos , Diatermia/instrumentação , Dissecação/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Fatores de Tempo
17.
Med Phys ; 6(4): 305-6, 1979.
Artigo em Inglês | MEDLINE | ID: mdl-481356

RESUMO

The generation of long, high quality random number sequences for Monte Carlo simulations using minicomputers is considered. The importance of the thorough testing of Monte Carlo random number generators is emphasized. A recommendation is given to authors of Monte Carlo papers to specify their random number generator and to describe the randomness testing which that generator has undergone.


Assuntos
Computadores , Método de Monte Carlo , Pesquisa Operacional , Radiometria
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