Super-resolution microscopy unveils the nanoscale organization and self-limiting clustering of CD47 in human erythrocytes.

The transmembrane protein CD47, an innate immune checkpoint protein, plays a pivotal role in preventing healthy erythrocytes from immune clearance. Our study utilized stochastic optical-reconstruction microscopy (STORM) and single-molecule analysis to investigate the distribution of CD47 on the human erythrocyte membrane. Contrary to previous findings in mouse erythrocytes, we discovered that CD47 exists in randomly distributed monomers rather than in clusters across the human erythrocyte membrane. Using 2nd antibody-induced crosslinking, we found that CD47 aggregates into stable clusters within minutes. By comparing these STORM results with those of the fully mobile protein CD59 and the cytoskeleton-bound membrane protein glycophorin C under similar conditions, as well as devising two-color STORM co-labeling and co-clustering experiments, we further quantitatively revealed an intermediate, self-limiting clustering behavior of CD47, elucidating its fractional (∼14%) attachment to the cytoskeleton. Moreover, we report reductions in both the amount of CD47 and its clustering capability in aged erythrocytes, providing new insight into erythrocyte senescence. Together, the combination of STORM and 2nd antibody-based crosslinking unveils the unique self-limiting clustering behavior of CD47 due to its fractional cytoskeleton attachment.

Optical vortex ladder via Sisyphus pumping of Pseudospin.

Robust high-order optical vortices are much in demand for applications in optical manipulation, optical communications, quantum entanglement and quantum computing. However, in numerous experimental settings, a controlled generation of optical vortices with arbitrary orbital angular momentum remains a challenge. Here, we present a concept of "optical vortex ladder" for the stepwise generation of optical vortices through Sisyphus pumping of pseudospin modes in photonic graphene. The ladder is applicable in various lattices with Dirac-like structures. Instead of conical diffraction and incomplete pseudospin conversion under conventional Gaussian beam excitations, the vortices produced in the ladder arise from non-trivial topology and feature diffraction-free Bessel profiles, thanks to the refined excitation of the ring spectrum around the Dirac cones. By employing a periodic "kick" to the photonic graphene, effectively inducing the Sisyphus pumping, the ladder enables tunable generation of optical vortices of any order even when the initial excitation does not involve any orbital angular momentum. The optical vortex ladder stands out as an intriguing non-Hermitian dynamical system, and, among other possibilities, opens a pathway for applications of topological singularities in beam shaping and wavefront engineering.

Electro-Optic Response of Polymer-Stabilized Cholesteric Liquid Crystals with Different Polymer Concentrations.

Polymer-stabilized cholesteric liquid crystals (PSCLCs) have emerged as promising candidates for one-dimensional photonic lattices that enable precise tuning of the photonic band gap (PBG). This work systematically investigates the effect of polymer concentrations on the AC electric field-induced tuning of the PBG in PSCLCs, in so doing it explores a range of concentrations and provides new insights into how polymer concentration affects both the stabilization of cholesteric textures and the electro-optic response. We demonstrate that low polymer concentrations (≈3 wt. %) cause a blue shift in the short wavelength band edge, while high concentrations (≈10 wt. %) lead to a contraction and deterioration of the reflection band. Polarization optical microscopy was conducted to confirm the phase transition induced by the application of an electric field. The observations confirm that increased polymer concentration stabilizes the cholesteric texture. Particularly, the highly desired fingerprint texture was stabilized in a sample with 10 wt. % of the polymer, whereas it was unstable for lower polymer concentrations. Additionally, higher polymer concentrations also improved the dissymmetry factor and stability of the lasing emission, with the dissymmetry factor reaching the value of around 2 for samples with 10 wt. % of polymer additive. Our results provide valuable comprehension into the design of advanced PSCLC structures with tunable optical properties, enhancing device performance and paving the way for innovative photonic applications.

Bulk photovoltaic effect in ferroelectric nematic liquid crystals.

The bulk photovoltaic (BPV) effect in ferroelectric liquid crystals is of increasing scientific interest owing to its great potential for light-energy conversion. The ferroelectric nematic phase exhibits a huge spontaneous polarization that can be aligned to a preferred direction. In this Letter, we investigate the tensorial properties of the BPV effect in the planarly aligned ferroelectric nematic phase of the liquid crystalline material RM734. A steady-state short-circuit photocurrent of ~160 pA and an open-circuit photovoltage of ~50 mV were observed in a cell with a thickness of 5.5 µm under the illumination of ultraviolet light without any bias voltage. Based on the photocurrent measurements in different electrode configurations, the non-zero elements of the BPV tensor were obtained. The BPV effect is attributed to the combination of the spontaneous polarization and the asymmetric distribution of photoinduced charge carriers. This study not only provides an understanding of the bulk PV mechanism in soft ferroelectrics but also promises a wide range of unprecedented, to the best of our knowledge, benefits for light harvesting to engineer marketable photovoltaic devices.

Giant Kerr nonlinearity of terahertz waves mediated by stimulated phonon polaritons in a microcavity chip.

Optical Kerr effect, in which input light intensity linearly alters the refractive index, has enabled the generation of optical solitons, supercontinuum spectra, and frequency combs, playing vital roles in the on-chip devices, fiber communications, and quantum manipulations. Especially, terahertz Kerr effect, featuring fascinating prospects in future high-rate computing, artificial intelligence, and cloud-based technologies, encounters a great challenge due to the rather low power density and feeble Kerr response. Here, we demonstrate a giant terahertz frequency Kerr nonlinearity mediated by stimulated phonon polaritons. Under the influences of the giant Kerr nonlinearity, the power-dependent refractive index change would result in a frequency shift in the microcavity, which was experimentally demonstrated via the measurement of the resonant mode of a chip-scale lithium niobate Fabry-Pérot microcavity. Attributed to the existence of stimulated phonon polaritons, the nonlinear coefficient extracted from the frequency shifts is orders of magnitude larger than that of visible and infrared light, which is also theoretically demonstrated by nonlinear Huang equations. This work opens an avenue for many rich and fruitful terahertz Kerr effect based physical, chemical, and biological systems that have terahertz fingerprints.

Omni-polarized Faraday isolator based on non-Hermitian Faraday system.

Non-Hermitian systems have recently attracted significant attention in photonics due to the realization that the interplay between gain and loss can lead to entirely new and unexpected features. Here, we propose and demonstrate a non-Hermitian Faraday system capable of non-reciprocal omni-polarizer action at the exceptional point. Notably, both forward and backward propagating light with arbitrary polarization converge to the same polarization state. Leveraging the robustness and non-reciprocity of the non-Hermitian Faraday system, we realize an omni-polarized Faraday isolator that can effectively isolate any polarized light without the need for a polarizer at the incident port of backward propagation. Remarkably, under the given parameter configuration, the isolator achieves a maximum isolation ratio of approximately 100 dB and a minimum isolation ratio of around 45 dB for various polarized light, accompanied by near-zero insertion loss. Furthermore, our research reveals the remarkable tolerance of the non-Hermitian Faraday isolator to nonlinear effects. This unique characteristic allows us to harness nonlinear effects to achieve various optical functions, all while maintaining excellent isolation performance. The proposed non-Hermitian Faraday system paves the way for the realization of magnetically or optically switchable non-reciprocal devices.

Transient cavity-cavity strong coupling at terahertz frequency on LiNbO3 chips.

Terahertz (THz) microcavities have garnered considerable attention for their ability to localize and confine THz waves, allowing for strong coupling to remarkably enhance the light-matter interaction. These properties hold great promise for advancing THz science and technology, particularly for high-speed integrated THz chips where transient interaction between THz waves and matter is critical. However, experimental study of these transient time-domain processes requires high temporal and spatial resolution since these processes, such as THz strong coupling, occur in several picoseconds and microns. Thus, most literature studies rarely cover temporal and spatial processes at the same time. In this work, we thoroughly investigate the transient cavity-cavity strong-coupling phenomena at THz frequency and find a Rabi-like oscillation in the microcavities, manifested by direct observation of a periodic energy exchange process via a phase-contrast time-resolved imaging system. Our explanation, based on the Jaynes-Cummings model, provides theoretical insight into this transient strong-coupling process. This work provides an opportunity to deeply understand the transient strong-coupling process between THz microcavities, which sheds light on the potential of THz microcavities for high-speed THz sensor and THz chip design.

Collective Cell Radial Ordered Migration in Spatial Confinement.

Collective cells, a typical active matter system, exhibit complex coordinated behaviors fundamental for various developmental and physiological processes. The present work discovers a collective radial ordered migration behavior of NIH3T3 fibroblasts that depends on persistent top-down regulation with 2D spatial confinement. Remarkably, individual cells move in a weak-oriented, diffusive-like rather than strong-oriented ballistic manner. Despite this, the collective movement is spatiotemporal heterogeneous and radial ordering at supracellular scale, manifesting as a radial ordered wavefront originated from the boundary and propagated toward the center of pattern. Combining bottom-up cell-to-extracellular matrix (ECM) interaction strategy, numerical simulations based on a developed mechanical model well reproduce and explain above observations. The model further predicts the independence of geometric features on this ordering behavior, which is validated by experiments. These results together indicate such radial ordered collective migration is ascribed to the couple of top-down regulation with spatial restriction and bottom-up cellular endogenous nature.

Electro-Optical Comb Envelope Engineering Based on Mode Crossing.

Resonator-enhanced electro-optical (EO) combs could generate a series of comb lines with high coherence and stability. Recently, EO comb based on thin-film lithium niobate (TFLN) has begun to show great potential thanks to the high second-order nonlinearity coefficient of lithium niobate crystal. Here we demonstrate that EO comb envelope engineering based on mode crossing induced a quality factor reduction in the TFLN racetrack microcavity both in the numerical simulation and experiment. Our method paves the way for the generation of EO combs with an arbitrary envelope.

Electrically Tunable Two-Color Cholesteric Laser.

Two-color lasing emission from an asymmetric structure, consisting of two dye-doped cholesteric liquid crystal (DD-CLC) layers separated by a transparent interlayer, is demonstrated. The DD-CLC mixtures have different reflection bands with long-wavelength band edges located at the green and red wavelengths of the visible spectrum, respectively. For the laser action, the CLC hosts provide the feedback, and the fluorescent laser dyes represent the active medium. When the stacked structure is optically pumped above the threshold, two simultaneous laser lines separated by 123 nm are observed at the long-wavelength band edges of the DD-CLC mixtures. The influence of an electric field on lasing behavior is also analyzed and discussed in terms of the reflection spectrum and laser action. The results show a reversible tuning of the reflection band, accompanied by a modification of the lasing characteristics under the application of an external field. Above a specific threshold voltage, one of the emission lines is suppressed and the other is conserved. With a further increase in the voltage, both laser emissions are entirely inhibited. The investigated structure demonstrates a simple technique to obtain an electrically tunable multi-wavelength laser, which might pave the way for a new generation of organic laser sources.

Equilibrium Dynamics of Mutually Confined Waves with Signed Analogous Masses.

We report the first experimental realization of equilibrium dynamics of mutually confined waves with signed analogous masses in an optical fiber. Our Letter is mainly demonstrated by considering a mutual confinement between a soliton pair and a dispersive wave experiencing opposite dispersion. The resulting wave-packet complex is found robust upon random perturbation and collision with other waves. The equilibrium dynamics are also extended to scenarios of more than three waves. Our finding may trigger fundamental interest in the dynamics of many-body systems arising from the concept of negative mass, which is promising for new applications based on localized nonlinear waves.

Controllable nonlinear propagation of partially incoherent Airy beams.

The self-accelerating beams such as the Airy beam show great potentials in many applications including optical manipulation, imaging and communication. However, their superior features during linear propagation could be easily corrupted by optical nonlinearity or spatial incoherence individually. Here we investigate how the interaction of spatial incoherence and nonlinear propagation affect the beam quality of Airy beam, and find that the two destroying factors can in fact balance each other. Our results show that the influence of coherence and nonlinearity on the propagation of partially incoherent Airy beams (PIABs) can be formulated as two exponential functions that have factors of opposite signs. With appropriate spatial coherence length, the PIABs not only resist the corruption of beam profile caused by self-focusing nonlinearity, but also exhibits less anomalous diffraction caused by the self-defocusing nonlinearity. Our work provides deep insight into how to maintain the beam quality of self-accelerating Airy beams by exploiting the interaction between partially incoherence and optical nonlinearity. Our results may bring about new possibilities for optimizing partially incoherent structured field and developing related applications such as optical communication, incoherent imaging and optical manipulations.

Photonic Realization of a Generic Type of Graphene Edge States Exhibiting Topological Flat Band.

Cutting a honeycomb lattice (HCL) ends up with three types of edges (zigzag, bearded, and armchair), as is well known in the study of graphene edge states. Here, we propose and demonstrate a distinctive twig-shaped edge, thereby observing new edge states using a photonic platform. Our main findings are (i) the twig edge is a generic type of HCL edge complementary to the armchair edge, formed by choosing the right primitive cell rather than simple lattice cutting or Klein edge modification; (ii) the twig edge states form a complete flat band across the Brillouin zone with zero-energy degeneracy, characterized by nontrivial topological winding of the lattice Hamiltonian; (iii) the twig edge states can be elongated or compactly localized at the boundary, manifesting both flat band and topological features. Although realized here in a photonic graphene, such twig edge states should exist in other synthetic HCL structures. Moreover, our results may broaden the understanding of graphene edge states, as well as new avenues for realization of robust edge localization and nontrivial topological phases based on Dirac-like materials.

4D Additive-Subtractive Manufacturing of Shape Memory Ceramics.

The development of high-temperature structural materials, such as ceramics, is limited by their extremely high melting points and the difficulty in building complicated architectures. Four-dimensional (4D) printing helps enhance the geometrical flexibility of ceramics. However, ceramic 4D printing systems are limited by the separate processes for shape and material transformations, low accuracy of morphing systems, low resolution of ceramic structures, and their time-intensive nature. Here, a paradigm for a one-step shape/material transformation, high-2D/3D/4D-precision, high-efficiency, and scalable 4D additive-subtractive manufacturing of shape memory ceramics is developed. Original/reverse and global/local multimode shape memory capabilities are achieved using macroscale SiOC-based ceramic materials. The uniformly deposited Al2 O3 -rich layer on the printed SiOC-based ceramic lattice structures results in an unusually high flame ablation performance of the complex-shaped ceramics. The proposed framework is expected to broaden the applications of high-temperature structural materials in the aerospace, electronics, biomedical, and art fields.

On-chip erbium-ytterbium-co-doped lithium niobate microdisk laser with an ultralow threshold.

Erbium-ion-doped lithium niobate (LN) microcavity lasers working in the communication band have attracted extensive attention recently. However, their conversion efficiencies and laser thresholds still have significant room to improve. Here, we prepared microdisk cavities based on erbium-ytterbium-co-doped LN thin film by using ultraviolet lithography, argon ion etching, and a chemical-mechanical polishing process. Benefiting from the erbium-ytterbium co-doping-induced gain coefficient improvement, laser emission with an ultralow threshold (∼1 µW) and high conversion efficiency (1.8 × 10-3%) was observed in the fabricated microdisks under a 980-nm-band optical pump. This study provides an effective reference for improving the performance of LN thin-film lasers.

Electrically tuned coupling of lithium niobate microresonators.

Microresonators coupled with integrated waveguides operate stably but usually lack tunability for an optimal coupling state. In this Letter, we demonstrate a racetrack resonator with an electrically modulated coupling on an X-cut lithium niobate (LN) platform by introducing a Mach-Zehnder interferometer (MZI) with two balanced directional couplers (DCs) to realize light exchange. This device provides a wide-range coupling regulation, from under-coupling and critical coupling to deep over-coupling. Importantly, it has a fixed resonance frequency when the DC splitting ratio is 3 dB. The measured optical responses of the resonator exhibit a high extinction ratio, exceeding 23 dB, and an effective half-wave voltage length Vπ·L of 0.77 V·cm, suitable for CMOS compatibility. Microresonators with tunable coupling and a stable resonance frequency are expected to find application in nonlinear optical devices on LN-integrated optical platforms.

On-chip ytterbium-doped lithium niobate waveguide amplifiers with high net internal gain.

Integrated optical systems based on lithium niobate on insulator (LNOI) have shown great potential in recent years. However, the LNOI platform is facing a shortage of active devices. Considering the significant progress made in rare-earth-doped LNOI lasers and amplifiers, the fabrication of on-chip ytterbium-doped LNOI waveguide amplifiers based on electron-beam lithography and inductively coupled plasma reactive ion etching was investigated. The signal amplification at lower pump power (<1 mW) was achieved by the fabricated waveguide amplifiers. A net internal gain of ∼18 dB/cm in the 1064 nm band was also achieved in the waveguide amplifiers under a pump power of 10 mW at 974 nm. This work proposes a new, to the best of our knowledge, active device for the LNOI integrated optical system. It may become an important basic component for lithium niobate thin-film integrated photonics in the future.

Second-harmonic and cascaded third-harmonic generation in generalized quasiperiodic poled lithium niobate waveguides.

Lithium niobate (LN) thin film has recently emerged as an important platform for nonlinear optical investigations for its large χ(2) nonlinear coefficients and ability of light localization. In this Letter, we report the first, to the best of our knowledge, fabrication of LN-on-insulator ridge waveguides with generalized quasiperiodic poled superlattices using the electric field polarization technique and microfabrication techniques. Benefiting from the abundant reciprocal vectors, we observed efficient second-harmonic and cascaded third-harmonic signals in the same device, with normalized conversion efficiency of 1735% W-1 cm-2 and 0.41% W-2 cm-4, respectively. This work opens a new direction for nonlinear integrated photonics based on LN thin film.

Mesenchymal Migration on Adhesive-Nonadhesive Alternate Surfaces in Macrophages.

Mesenchymal migration usually happens on adhesive substrates, while cells adopt amoeboid migration on low/nonadhesive surfaces. Protein-repelling reagents, e.g., poly(ethylene) glycol (PEG), are routinely employed to resist cell adhering and migrating. Contrary to these perceptions, this work discovers a unique locomotion of macrophages on adhesive-nonadhesive alternate substrates in vitro that they can overcome nonadhesive PEG gaps to reach adhesive regions in the mesenchymal mode. Adhering to extracellular matrix regions is a prerequisite for macrophages to perform further locomotion on the PEG regions. Podosomes are found highly enriched on the PEG region in macrophages and support their migration across the nonadhesive regions. Increasing podosome density through myosin IIA inhibition facilitates cell motility on adhesive-nonadhesive alternate substrates. Moreover, a developed cellular Potts model reproduces this mesenchymal migration. These findings together uncover a new migratory behavior on adhesive-nonadhesive alternate substrates in macrophages.

Dual-Wavelength Lasing with Orthogonal Circular Polarizations Generated in a Single Layer of a Polymer-Cholesteric Liquid Crystal Superstructure.

We investigate the laser emission from a polymer-cholesteric liquid crystal superstructure with coexisting opposite chiralities fabricated by refilling a right-handed polymeric scaffold with a left-handed cholesteric liquid crystalline material. The superstructure exhibits two photonic band gaps corresponding to the right- and left-circularly polarized light. By adding a suitable dye, dual-wavelength lasing with orthogonal circular polarizations is realized in this single-layer structure. The wavelength of the left-circularly polarized laser emission is thermally tunable, while the wavelength of the right-circularly polarized emission is relatively stable. Due to its relative simplicity and tunability characteristics, our design might have broad application prospects in various fields of photonics and display technology.