Bisphenol A induces lipid metabolism disorder and impairs hepatopancreas of Sesarmops sinensis.

Bisphenol A (BPA) is a chemical that disrupts the endocrine system and may have negative implications on the lipid metabolism of organisms. To ascertain BPA implications on lipid metabolism in the hepatopancreas of Sesarmops sinensis, we exposed S. sinensis to different concentrations of BPA for 14 days. The outcomes manifested that BPA may stimulate hepatopancreas injury and lipid deposition in the hepatopancreas of S. sinensis and lead to the increase of hepatosomatic index (HSI). Transcriptome analysis showed that lipid metabolism-related pathways were significantly enriched in KEGG pathways. BPA exposure also caused disorders in lipid metabolism by altering fatty acid composition and lipid metabolites. The up-regulation of lipid synthesis genes and the alteration of lipid transport genes may be important reasons for the disorder of lipid metabolism. Furthermore, these outcomes provide a fresh point of reference for comprehending the ecotoxicological impacts of BPA on aquatic organisms.

Hexavalent chromium damages intestinal cells and coelomocytes and impairs immune function in the echiuran worm Urechis unicinctus by causing oxidative stress and apoptosis.

Hexavalent chromium (Cr(VI)) is a common pollutant in the marine environment, which impairs immunity and causes reproductive and heredity disorders in organisms. To clarify the immunotoxic effects of Cr (VI) on the marine worm Urechis unicinctus, we analyzed tissue damage and immune dysfunction caused by Cr (VI) in this organism at histopathologic, zymologic, apoptotic and molecular levels. The results indicated that the bioaccumulation of Cr (VI) bioaccumulation levels in coelomocytes was significantly higher than in the intestines and muscles. Pathological observation showed that Cr (VI) caused damage to the respiratory intestine, stomach and midgut. Cr (VI) also increased the replication of goblet cells and a reduction in the replication of epithelial cells. Meanwhile, Cr (VI) induced apoptosis of intestinal cells and coelomocytes, accompanied by an increase in the expression of Caspase-3, COX-2, and MyD88 in the intestine and coelomocytes. At the same time, Cr (VI) significantly affected the activities of antioxidant enzymes such as SOD, ACP, CAT, CAT, and GST, and increased H2O2 and MDA contents in U. unicinctus. Moreover, Cr (VI) exposure also up-regulated the transcription of hsc70, mt and jnk genes but decreased that of sod in the intestines. In contrast, Cr (VI) down-regulated the expression of sod, hsc70, mt, and jnk genes in coelomocytes. Collectively, Cr (VI) bioaccumulated in U. unicinctus cells and tissues, causing several histopathological changes, oxidative stress, and apoptosis of several cells in the organism, resulting in intestinal and coelomocyte damage and immune dysfunctioning.

Toxic effect of chromium on nonspecific immune, bioaccumulation, and tissue structure of Urechis unicinctus.

The contamination of toxic heavy metals in aquatic environments has garnered significant global attention due to its detrimental effects on marine organisms and human health. Hexavalent chromium is a typical environmental and occupational heavy metal pollutant, identified as carcinogenic heavy metal. This study aimed to assess the impact of different Cr (VI) concentrations (0.05-2.5 mg/L) on Urechis unicinctus (U. unicinctus) by investigating bioaccumulation, antioxidant defense system, expression of resistance-related genes, and histological issues. A clear concentration-effect relationship was observed in the bioaccumulation of Cr (VI) in muscle tissues of U. unicinctus. Moreover, exposure to Cr (VI) can alter the activities of lysozyme (LSZ), catalase (CAT), and superoxide dismutase (SOD) to enhance cellular defense mechanisms in U. unicinctus. Likewise, maintained the normal protein structure and functional stability by regulating protein folding. The heat shock cognitive protein (HSC70) gene showed an upward and then downward trend after Cr (VI) exposure. At 12 h, the HSC70 gene expression reached the maximum values of 4.75 and 4.61-fold in the 0.1 and 1.5 mg/L groups, respectively. The organism produced a large number of free radicals, and elevated level of metallothionein (MT) was used to scavenge free radicals and alleviate oxidative stress. Additionally, histopathological examination revealed disorganization in the midgut, atrophic changes in intestinal connective tissue, uneven distribution in respiratory tissues, and irregular shape with a significant reduction in epithelial cells within the gastric cavity. These findings can serve as a valuable reference for elucidating the toxicity mechanisms of heavy metals towards marine benthic organisms and enhancing water environment monitoring strategies.

The development of local ambient air quality standards: A case study of Hainan Province, China.

The ambient air quality standard (AAQS) is a vital policy instrument for protecting the environment and human health. Hainan Province is at the forefront of China's efforts to protect its ecological environment, with an official goal to achieve world-leading air quality by 2035. However, neither the national AAQS nor the World Health Organization guideline offers sufficient guidance for improving air quality in Hainan because Hainan has well met the former while the latter is excessively stringent. Consequently, the establishment of Hainan's local AAQS becomes imperative. Nonetheless, research regarding the development of local AAQS is scarce, especially in comparatively more polluted countries such as China. The relatively high background values and significant interannual fluctuations in air pollutant concentrations in Hainan present challenges in the development of local AAQS. Our research proposes a world-class local AAQS of Hainan Province by reviewing the AAQS in major countries or regions worldwide, analyzing the influence of different statistical forms, and carefully evaluating the attainability of the standard. In the proposed AAQS, the annual mean concentration limit for PM2.5, the annual 95th percentile of daily maximum 8-h mean (MDA8) concentration limit for O3, and the peak season concentration limit for O3 are set at 10, 120, and 85 µg/m3, respectively. Our study indicates that, with effective control policies, Hainan is projected to achieve compliance with the new standard by 2035. The implementation of the local AAQS is estimated to avoid 1,526 (1,253-1,789) and 259 (132-501) premature deaths attributable to long-term exposure to PM2.5 and O3 in Hainan in 2035, respectively.

Nonspecific immune, histology and accumulation of marine worm, Urechis unicinctus in response to bisphenol A (BPA).

Bisphenol A (BPA) is one of the environmental endocrine disruptors, due to its chemical stability it exists in abundant concentrations in water and soil consequently accumulating in the food chain and causing many endocrine-related health problems. So far, studies on the effects of BPA on marine invertebrates have focused on acute toxicity, endocrine regulation, reproduction, and development. However, fewer studies have been conducted on marine benthos. The current study aimed to detect the accumulation of BPA and its impact on tissue structure, antioxidant capacity, and immune indexes in marine worm, Urechis unicinctus. U. unicinctus, as a common marine benthic animal, were exposed to different concentrations of BPA. Blood cells and intestinal tract were taken for tissue structure inspection, and supernatant of the coelomic fluid was collected for oxidative and antioxidant biomarkers. Results showed that the accumulation of BPA in muscles of U. unicinctus tended to increase with exposure time. BPA induced a rise in H2O2 and MDA content, and altered the activities of CAT, T-SOD, GST, LSZ and ACP, weaken the immune system functions. Moreover, pathological observation showed that BPA caused severe histopathology in the respiratory intestine, stomach, and midgut. These results will be helpful to understand the response mechanism of U. unicinctus under BPA exposure and provide a reference for controlling the aquaculture conditions and marine water quality of U. unicinctus.

The Effectiveness of Preventive Nursing Interventions in Patients on Hemodialysis with Arteriovenous Fistulas and Their Value in Preventing Complications.

Objective: To analyze the effectiveness of preventive nursing interventions in patients on hemodialysis with arteriovenous fistulas (AVF) and the value in preventing complications of the AVF. Method: It was a randomized controlled trial that enrolled 60 patients on hemodialysis treated in our hospital from April 2019 to May 2021. All enrollments were divided into control group (n = 30) and study group (n = 30) by random table methods. The control group received conventional nursing methods, while the study group received preventive nursing methods. The incidence of AVF complications, blood flow in the AVF, levels of anxiety and depression, quality of life and nursing satisfaction were assessed and compared. Results: The incidence of AVF complications in the study group after intervention was significantly lower than that in the control group. Blood flow in the AVF was much higher in the study group than in the control group. The anxiety and depression levels of patients in the study group after intervention were both lower than those of patients in the control group. Patients in the study group had a much better quality of life after intervention than those in the control group. The nursing satisfaction of patients in the study group after intervention was also higher than that of patients in the control group. In other words, patients in the study group were more satisfied with the way they had been cared for compared to those in the control group. Conclusion: Preventive nursing interventions are effective in patients on hemodialysis. Not only do they reduce the incidence of AVF complications, they also improve blood flow in the AVF. In addition, they reduce such negative emotions as anxiety and depression, improves the patient's quality of life, as well as his or her satisfaction with care provision.

Different stages of chronic kidney disease are associated with physical performance in adults over 60 years.

Objective: The purpose of this study was to determine the association between different stages of chronic kidney disease (CKD) and sarcopenia and its components in the Chinese older population. Methods: The study comprised of 2,213 participants aged ≥ 60 years (1,025 men; mean age: 70.7 years) recruited from Shanghai who were invited to participate in a comprehensive geriatric assessment. Sarcopenia was defined according to the AWGS 2019 consensus update on sarcopenia diagnosis criteria. The glomerular filtration rate (GFR) was estimated using the equation that originated from the CKD-EPI equation, the stages of CKD are classified according to the Kidney Disease-Improving Global Outcomes (KDIGO). Results: The overall prevalence of sarcopenia was 19.0%, which increased with the severity of CKD. The prevalence of sarcopenia in patients with CKD 3-4 and kidney failure was significantly higher than that in normal and CKD 1-2 (p < 0.05). In logistic regression analysis model, compared with normal and CKD 1 patients, kidney failure was significantly associated with the increased risk of sarcopenia and low grip strength (p < 0.05); CKD 2, CKD 3-4 and kidney failure groups were significantly associated with an increased risk of low walking speed (p < 0.05), respectively; while the association between CKD and muscle mass was not shown. Conclusions: In our study, only decreased physical performance, as represented by walking speed, was significantly associated with increased CKD severity. This may improve the evidence for the prevention and intervention of sarcopenia in patients with CKD.

Changes in biochemical composition and digestive enzyme activity during the embryonic development of the marine crab, Charybdis japonica (Crustacea: Decapoda).

In this study, we investigated the biochemical composition and digestive enzyme activity during embryonic development in the marine crab, Charybdis japonica. Water, protein, and ash content increased, while levels of lipids and carbohydrates decreased significantly during embryonic development, and a marked correlation between water content and egg volume (r = 0.97; P < 0.01) was recorded. The utilization percentages of lipids and carbohydrates were 32.72% and 91.56%, respectively. The predominant essential amino acids (EAA) were lysine, leucine, arginine and valine, and the major nonessential amino acids (NEAA) were glutamic acid and aspartic acid. From the fertilized egg stage to the protozoea stage, total amino acid (TAA) concentration increased from 52.18% to 55.11% on a dry weight basis, but the ratio of EAA/TAA decreased from 52.57% to 48.90%. The quantitatively more important fatty acids were C16:0, C18:1n-9c, C16:1, C22:6n-3 (docosahexaenoic acid, DHA), and C22:2. Polyunsaturates (PUFA) and monounsaturated (MUFA) were consumed at similar rates (34.20% and 36.70%, respectively); both were consumed at higher rates than saturates (SFA) (26.56%). In particuler, n-3 fatty acids decreased significantly, with a high consumption rate of 43.74%. Activities of trypsin and pepsin increased during both the early and later embryonic stages, but decreased during the middle stages. Lipase activity increased gradually during embryonic development, except in the protozoea stage with a significant decrease, while activities of amylase and cellulase showed an ascending trend after an initial decline. The activity of all digestive enzymes increased, except for that of lipase, from the heartbeat stage to the protozoea stage.