Glis2 inhibits the epithelial-mesenchymal transition and apoptosis of renal tubule cells by regulating the ß-catenin signalling pathway in diabetic kidney disease.

Increasing evidence has supported the idea that epithelial-to-mesenchymal transition (EMT)-based tubulointerstitial fibrosis and the apoptosis of renal tubular epithelial cells (TECs) play important roles in the occurrence and development of Diabetic kidney disease (DKD). Glis2 is abundantly expressed in renal tubules and is a member of the Kruppel-like zinc finger transcription factor family, which is involved in the regulation of normal renal development and function. Glis2 deficiency may be closely associated with tubular atrophy and fibrosis, but the role played by Glis2 in DKD remains unclear. In this study, we found that Glis2 protein expression was downregulated in kidney tissue samples obtained by biopsy from DKD patients as well as HK-2 cells cultured in high-glucose medium, and overexpression of the Glis2 plasmid inhibited the apoptosis and EMT of TECS under HG conditions. In addition, Glis2 overexpression obliterated the activation of the ß-catenin signalling pathway in HG-cultured HK-2 cells. Moreover, the ß-catenin inhibitor XAV939 or XAV939 combined with Glis2 overexpression markedly inhibited the apoptosis and EMT of HG-treated HK-2 cells. All these findings indicated that upregulation of Glis2 expression might attenuate the EMT and apoptosis of renal tubule cells via the ß-catenin signalling pathway under HG conditions. This outcome may lead to a better understanding of the pathogenesis of DKD and provide new insights into prevention and treatment strategies targeting DKD.

Two-dimensional silicon photonic grating coupler with low polarization-dependent loss and high tolerance.

We design and demonstrate a two-dimensional grating coupler with an elliptical-like etching pattern on the silicon-on-insulator platform. The polarization-dependent loss of the fabricated device is measured to be 0.2 dB at the C-band, lower than that of most conventional 2D GCs. The etching unit can be easily fabricated with a large tolerance of ± 10 nm and a large feature size of 310nm, which is beneficial to its manufacturing process and wide application.

Mode measurement of few-mode fibers by mode-frequency mapping.

Since few-mode fibers (FMFs) have great potential as the new transmission media for optical communications, the ability to distinguish different fiber modes is essential. Most of the traditional schemes do not yield phase information, or are limited by beam size and mechanical requirements. Here, a method is presented to analyze the mode distribution of FMFs. The fiber modes are mapped to different frequencies by using dynamic spatial phase masks. The complex amplitudes at these frequencies indicate the amplitudes and phases of the fiber modes. The method can extract not only the amplitude distribution, but also the phase distribution of the fiber modes, and no other assisted light is needed.