RESUMO
ABSTRACT: Background : It is reported that circVMA21 has an inhibition effect on sepsis-induced acute kidney injury (AKI). Therefore, the underlying molecular mechanisms of circVMA21 in AKI are worthy of further investigation. Material and Methods : Lipopolysaccharide (LPS) was used to induce HK2 cell injury. CircVMA21, miR-337-3p and ZEB2 expression was tested by qRT-PCR. Cell growth was detected by CCK8 assay, EdU assay, and flow cytometry. Protein levels were examined by western blot. The levels of inflammatory factors and oxidative stress markers were measured to evaluate cell inflammatory response and oxidative stress. RNA relationship as verified by dual-luciferase reporter assay, RIP assay, and RNA pull-down assay. Results : CircVMA21 had decreased expression in AKI patients. Overexpressed circVMA21 alleviated LPS-induced HK2 cell inflammation, apoptosis, and oxidative stress. Moreover, circVMA21 sponged miR-337-3p, and miR-337-3p targeted ZEB2. The inhibitory effect of circVMA21 on LPS-induced HK2 cell injury was reversed by miR-337-3p overexpression, and ZEB2 overexpression abolished the promotion effect of miR-337-3p on LPS-induced HK2 cell injury. Conclusions : CircVMA21 could inhibit LPS-induced HK2 cell injury via miR-337-3p/ZEB2 axis.
Assuntos
Injúria Renal Aguda , Lipopolissacarídeos , MicroRNAs , RNA Circular , Homeobox 2 de Ligação a E-box com Dedos de Zinco , Lipopolissacarídeos/toxicidade , Humanos , Injúria Renal Aguda/metabolismo , Injúria Renal Aguda/patologia , RNA Circular/genética , RNA Circular/metabolismo , MicroRNAs/metabolismo , MicroRNAs/genética , Homeobox 2 de Ligação a E-box com Dedos de Zinco/metabolismo , Homeobox 2 de Ligação a E-box com Dedos de Zinco/genética , Linhagem Celular , Estresse Oxidativo , Apoptose/efeitos dos fármacosRESUMO
Ulcerative colitis is a major inflammatory bowel disease (IBD), characterized by inflammation within the gastrointestinal tract through chronic or relapsing immune system activation. The aim of this study is to investigate the potential protective effect of oat ß-glucan (ßG) against colitis induced by DSS in mice. Eighty mice were randomly divided into the control group (no DSS, no ßG), DSS group (DSS only), DSS + L-ßG group (DSS plus 500 mg per kg ßG), and DSS + H-ßG group (DSS plus 1000 mg per kg ßG). Compared with the DSS group, administration of ßG significantly reduced clinical symptoms with less weight loss, diarrhea and shortening of the colon, the severity of colitis was significantly inhibited as evidenced by the reduced disease activity index (DAI) and degree of histological damage in colon. Moreover, treatment with ßG not only decreased myeloperoxidase activity (MPO), and nitric oxide (NO) and malondialdehyde (MDA) levels, but also inhibited mRNA and protein expression of pro-inflammatory factors such as TNF-α, IL-1ß, IL-6 and iNOS. This suggests that oat ßG in diet might exhibit an anti-inflammatory function against colitis through inhibition of expression of pro-inflammatory factors.
