Fuzzy random sensitivity analysis for the overall structure reliability of reinforced concrete freezing wellbores in deep alluvium based on hidden Markov model.

To address the shortcomings of traditional reliability theory in characterizing the stability of deep underground structures, the advanced first order second moment of reliability was improved to obtain fuzzy random reliability, which is more consistent with the working conditions. The traditional sensitivity analysis model was optimized using fuzzy random optimization, and an analytical calculation model of the mean and standard deviation of the fuzzy random reliability sensitivity was established. A big data hidden Markov model and expectation-maximization algorithm were used to improve the digital characteristics of fuzzy random variables. The fuzzy random sensitivity optimization model was used to confirm the effect of concrete compressive strength, thick-diameter ratio, reinforcement ratio, uncertainty coefficient of calculation model, and soil depth on the overall structural reliability of a reinforced concrete double-layer wellbore in deep alluvial soil. Through numerical calculations, these characteristics were observed to be the main influencing factors. Furthermore, while the soil depth was negatively correlated, the other influencing factors were all positively correlated with the overall reliability. This study provides an effective reference for the safe construction of deep underground structures in the future.

Human papillomavirus and cervical cancer in the microbial world: exploring the vaginal microecology.

The vaginal microbiota plays a crucial role in female reproductive health and is considered a biomarker for predicting disease outcomes and personalized testing. However, its relationship with human papillomavirus (HPV) infection and cervical cancer is not yet clear. Therefore, this article provides a review of the association between the vaginal microbiota, HPV infection, and cervical cancer. We discuss the composition of the vaginal microbiota, its dysbiosis, and its relationship with HPV infection, as well as potential mechanisms in the development of cervical cancer. In addition, we assess the feasibility of treatment strategies such as probiotics and vaginal microbiota transplantation to modulate the vaginal microbiota for the prevention and treatment of diseases related to HPV infection and cervical cancer. In the future, extensive replication studies are still needed to gain a deeper understanding of the complex relationship between the vaginal microbiota, HPV infection, and cervical cancer, and to clarify the role of the vaginal microbiota as a potential biomarker for predicting disease outcomes, thus providing a theoretical basis for personalized testing.

Clinical significance of serum oxidative stress and serum uric acid levels before surgery for hepatitis B-related liver cancer.

BACKGROUND: The incidence and mortality of liver cancer are among the highest of all malignant tumors in China. The high recurrence rate after conventional hepatectomy is worrying. There is a lack of effective prognostic indicators for liver cancer. AIM: To explore the clinical significance of preoperative serum oxidative stress and serum uric acid (UA) levels in hepatitis B-related liver cancer. METHODS: The medical records of 110 hepatitis B-related liver cancer patients who underwent hepatectomy in Gansu Provincial Hospital were retrospectively analyzed. Recurrence in patients within 3 years after surgery was determined. The logistic regression model and Pearson or Spearman correlation were used to analyze the correlation between oxidative stress level and UA, and the recurrence of hepatitis B-related liver cancer. RESULTS: Compared with the non-recurrence group, the levels of superoxide dismutase (SOD) and glutathione (GSH) in the recurrence group were lower and the levels of malondialdehyde (MDA) and UA were higher (all P < 0.05). UA, SOD, MDA, and GSH were risk factors for postoperative recurrence in hepatitis B-related liver cancer patients (P < 0.05). UA was positively correlated with MDA (r = 0.395, P < 0.001) and negatively correlated with GSH (r = -0.204, P = 0.032). The area under the receiver operating characteristic curve (AUC) of SOD, MDA, GSH, and UA in predicting the prognosis was 0.276, 0.910, 0.199, and 0.784, respectively (all P < 0.001). CONCLUSION: The preoperative serum SOD, GSH, MDA, and UA levels had significant predictive effects on postoperative recurrence of hepatitis B-related liver cancer.

Serum ANGPTL4 and SIRT1 factor levels and the Carotid Atherosclerotic plaque stability relationship analysis.

This study was to investigate the relationship between the levels of Angiopoietin-Like Protein 4 (ANGPTL4) and Silent Mating-type Information Regulation 2 Homolog 1 (SIRT1) and the stability of carotid atherosclerotic plaque. For this purpose, 108 patients with coronary heart disease in our hospital from Jan 2021 to May 2022 were selected as the coronary heart disease (CHD) group and 80 patients with the healthy examination as the control group. Patients' serum levels of ANGPTL4 and SIRT1 were collected, and their stability of carotid atherosclerotic plaque was determined by carotid ultrasound. According to their stability results, patients were divided into three subgroups: No plaque, Stable plaque, and Unstable plaque. The serum ANGPTL4 and SIRT1 levels were analyzed in different groups, and the correlation between their serum levels and the stability of carotid atherosclerotic plaque was analyzed by rank correlation. Results showed that the CHD group's serum ANGPTL4 and SIRT1 levels were lower, with statistical significance (P<0.05); A statistically significant difference in serum ANGPTL4 and SIRT1 levels were observed among patients with No plaques, Stable plaques, and Unstable plaques (P<0.05); A negative correlation was observed between serum levels of ANGPTL4 and SIRT1 and the stability of carotid atherosclerotic plaque (r=-0.438, -0.717, P<0.001); Serum ANGPTL4 and SIRT1 can be used as the evaluation method of carotid atherosclerotic plaque stability. When ANGPTL4 ≤ 30.17mg/L and SIRT1 ≤ 6.91µg/L, patients were more likely to develop unstable plaques; When ANGPTL4 ≤ 30.40mg/L and SIRT1 ≤ 6.87µg/L, patients were more likely to develop plaques (instability and/or stability). In conclusion, the serum levels of ANGPTL4 and SIRT1 in patients with CHD decreased. ANGPTL4 and SIRT1 will participate in the formation and development of carotid plaque, which can be used as a serological evaluation index to evaluate the occurrence and carotid atherosclerotic plaque's stability.

Fuzzy random evaluation of creep model of frozen soft soil in metro tunnel construction using artificial ground freezing technique.

Mastering the creep characteristics of artificial frozen soil and scientifically evaluating the creep model is an important guarantee for the safety of subway tunnel freezing construction. Base on the construction of Nantong metro tunnel, the uniaxial compressive strength tests of the artificially frozen soft soil were carried out to obtain the influence law of temperature on the uniaxial compressive strength, and the uniaxial creep tests were carried out to obtain the influence law of temperature and stress grade on creep, at - 5, - 10 and - 15 °C. The experimental results show that the creep characteristics of frozen soft soil specimens have obvious fuzzy randomness. The traditional ant colony algorithm is improved by optimizing the pheromone fuzzification coefficient, which improves the search efficiency and avoids the local optimum effectively. Subsequently, the improved fuzzy ant colony algorithm is used to invert the flexibility parameters of commonly used permafrost creep models. The fuzzy weight of evaluation index and the fuzzy random evaluation matrix were determined to evaluate the optimal creep model under three different stress levels of frozen soft soil. Finally, the reliability of the fuzzy random evaluation method was verified by engineering measured data.

Relationship between Cervicovaginal Microecological Changes and HPV16/18 Infection and Cervical Cancer in Women of Childbearing Age.

OBJECTIVE: The current study aimed to investigate the correlation between HPV16/18 infection and the microecological characteristics of the female reproductive tract and cervical lesions and to explore the risk factors associated with cervical precancerous lesions (CIN) and cervical cancer (CC). METHODS: A total of 326 women were selected for HPV screening, with 121 testing negative for HPV, 113 infected with HPV16/18, and 92 infected with other types of HPV. Microecological characteristics of the vaginal flora in all subjects were analyzed. Liquid-based thin layer cell (TCT) tests, genitourinary tract infection pathogen (STDs) assessments, HPV typing, and colposcopic pathological biopsies of exfoliated cervical cells were conducted. RESULTS: Among patients with HPV infection, there was a higher detection rate of abnormal microecological indicators such as bacterial vaginosis (BV) and vaginal cleanliness. Additionally, an increased proportion of vaginal microbiota (VM) imbalance was observed. Ureaplasma urealyticum (Uu) infection in the reproductive tract was closely associated with HPV 16/18 infection and showed co-infection. Moreover, patients with BV infection and high expression of HPV mRNA were at a higher risk of persistent HPV16/18 positive infection. BV infection, Uu infection, and HPV16/18 positive infection were identified as risk factors for CIN and CC. Furthermore, BV and Uu infections promoted the development of CIN/CC in patients infected with HPV16/18. CONCLUSIONS: Changes in vaginal microecology are strongly linked to HPV16/18 infection. BV infection, Uu infection, HPV viral load, and HPV16/18 infection are risk factors for CIN/CC. Timely treatment of BV and Uu infections, restoration of a normal vaginal microecological environment, and improvement of HPV16/18 outcomes can delay the occurrence and progression of CIN/CC.

The Exosomes Containing LINC00461 Originated from Multiple Myeloma Inhibit the Osteoblast Differentiation of Bone Mesenchymal Stem Cells via Sponging miR-324-3p.

Multiple myeloma is one of the hematological malignancies and inhibited osteoblast differentiation of bone marrow mesenchymal stem cells (BM-MSCs) which has been proved as a major complication of the patients with multiple myeloma. However, the pathomechanism of symptom remains unclear. Besides, several studies have indicated that LINC00461 plays an important role in the progression of multiple tumors. Hence, this study attempted to reveal the role of LINC00461 in the osteoblast differentiation of MSCs. In this study, the expression level of LINC00461 in the exosomes of multiple myeloma cells was measured, and BM-MSCs were cultured with the exosomes to observe the change of cellular phenotype. Moreover, downstream target of LINC00461 was searched and verified with dual-luciferase reporter assay, and the activation of the Wnt/ß-catenin pathway was also observed by Western blot. The results showed that the isolated BMSCs exhibited special biomarkers of MSCs. LINC00461 was significantly upregulated in the exosomes originated multiple myeloma cells, and increased LINC00461 significantly impeded the osteoblast differentiation of MSCs. Moreover, LINC00461 could significantly suppress the activation of the Wnt/ß-catenin pathway in MSCs. In conclusion, this study suggested that LINC00461 in exosomes of multiple myeloma could reduce the activity of the Wnt/ß-catenin pathway to inhibit the osteoblast differentiation of BM-MSCs via targeting miR-324-3p.