Different responses of soil bacterial community to plant-plant interactions under organic-inorganic fertilizers affect seedling establishment during subalpine forest succession.

Introduction: Rhizosphere bacterial community as a valuable indicator of soil quality and function, has been widespread studied. However, little knowledge is about the response of bacterial communities to plant-plant interaction and different fertilizers during secondary forest succession. Methods: We conducted a field pot experiment applying organic and inorganic fertilizers to monocultures and mixed cultures of dominant plant species from mid- to late-successional stages (Salix oritrepha, Betula albosinensis, and Picea asperata), and investigated the responses of plant growth and rhizosphere bacterial communities. Results and discussion: Results indicated that growth rate of plant height varied among plant species, but no significant differences were observed in soil bacterial diversity and composition among plant species or inter-specific interactions under control. Compared to control, inorganic fertilizer resulted in increases in plant growth and the relative abundance of Proteobacteria, Patescibacteria, Bacteroidetes and Gemmatimonadetes, while simultaneously leading to decrease in the relative abundance of Acidobacteria, Actinobacteria, Chloroflexi, Rokubacteria and Planctomycetes. When grown with other species, the bacterial communities in the mixture resembled those of S. oritrepha in singular monoculture under inorganic fertilizer treatment, but plant growth was not affected by interspecific interaction. Unlike inorganic fertilizer, organic fertilizer significantly affected bacterial communities and increased bacterial diversity, but did not alter the effects of plant-plant interactions on bacterial communities. It was also observed that organic fertilizer facilitated later successional species' growth (P. asperata and B. albosinensis) by the mid-successional species (S. oritrepha), ultimately facilitating secondary forest succession. In addition, plants at different successional stages harbor specific bacterial communities to affect their growth, and the bacterial communities contributed more than soil properties to the variations in the plant growth of S. oritrepha and P. asperata though the bacterial communities were regulated by soil factors. This finding highlights the significance of the rhizosphere bacteria on plant growth and plant community succession. It also emphasize the importance of considering both plant-plant interactions and diverse fertilizer types in forest restoration efforts and provide valuable insights into optimizing agronomic practices for secondary forest succession.

Differential responses of soil bacteria, fungi and protists to root exudates and temperature.

The impact of climate warming on soil microbes has been well documented, with studies revealing its effects on diversity, community structure and network dynamics. However, the consistency of soil microbial community assembly, particularly in response to diverse plant root exudates under varying temperature conditions, remains an unresolved issue. To address this issue, we employed a growth chamber to integrate temperature and root exudates in a controlled experiment to examine the response of soil bacteria, fungi, and protists. Our findings revealed that temperature independently regulated microbial diversity, with distinct patterns observed among bacteria, fungi, and protists. Both root exudates and temperature significantly influenced microbial community composition, yet interpretations of these factors varied among prokaryotes and eukaryotes. In addition to phototrophic bacteria and protists, as well as protistan consumers, root exudates determined to varying degrees the enrichment of other microbial functional guilds at specific temperatures. The effects of temperature and root exudates on microbial co-occurrence patterns were interdependent; root exudates primarily simplified the network at low and high temperatures, while responses to temperature varied between single and mixed exudate treatments. Moreover, temperature altered the composition of keystone species within the microbial network, while root exudates led to a decrease in their number. These results emphasize the substantial impact of plant root exudates on soil microbial community responses to temperature, underscoring the necessity for future climate change research to incorporate additional environmental variables.

Enhancing Zn2+ Storage Performance by Constructing the Interfaces Between VO2 and Co-N-C Layers.

Vanadium oxides have aroused attention as cathode materials in aqueous zinc-ion batteries (AZIBs) due to their low cost and high safety. However, low ion diffusion and vanadium dissolution often lead to capacity decay and deteriorating stability during cycling. Herein, vanadium dioxides (VO2) nanobelts are coated with a single-atom cobalt dispersed N-doped carbon (Co-N-C) layer via a facile calcination strategy to form Co-N-C layer coated VO2 nanobelts (VO2@Co-N-C NBs) for cathodes in AZIBs. Various in-/ex situ characterizations demonstrate the interfaces between VO2 layers and Co-N-C layers can protect the VO2 NBs from collapsing, increase ion diffusion, and enhance the Zn2+ storage performance. Additional density functional theory (DFT) simulations demonstrate that Co─O─V bonds between VO2 and Co-N-C layers can enhance interfacial Zn2+ storage. Moreover, the VO2@Co-N-C NBs provided an ultrahigh capacity (418.7 mAh g-1 at 1 A g-1), outstanding long-term stability (over 8000 cycles at 20 A g-1), and superior rate performance.

The contrasting responses of abundant and rare microbial community structures and co-occurrence networks to secondary forest succession in the subalpine region.

Knowledge of variations in abundant and rare soil microbial communities and interactions during secondary forest succession is lacking. Soil samples were gathered from different secondary successional stages (grassland, shrubland, and secondary forest) to study the responses of abundant and rare bacterial and fungal communities, interactions and driving factors to secondary forest succession by Illumina sequencing of the 16S and ITS rRNA genes. The results showed that the α-diversities (Shannon index) of abundant bacteria and fungi revealed no significant changes during secondary forest succession, but increased significantly for rare bacteria. The abundant and rare bacterial and fungal ß-diversities changed significantly during secondary forest succession. Network analysis showed no obvious changes in the topological properties (nodes, links, and average degree) of abundant microbial networks during secondary forest succession. In contrast, these properties of the rare microbial networks in the secondary forest were higher than those in the grassland and shrubland, indicating that rare microbial networks are more responsive to secondary forest succession than abundant microorganisms. Additionally, rare microbial networks revealed more microbial interactions and greater network complexity than abundant microbial networks due to their higher numbers of nodes and links. The keystone species differed between the abundant and rare microbial networks and consisted of 1 and 48 keystone taxa in the abundant and rare microbial networks, respectively. Soil TP was the most important influencing factor of abundant and rare bacterial communities. Successional stages and plant richness had the most important influences on abundant and rare fungal communities, respectively. C:P, SM and N:P were mainly related to abundant and rare microbial network topological properties. Our study indicates that abundant and rare microbial communities, interactions and driving factors respond differently to secondary forest succession.

Variations in the soil micro-food web structure and its relationship with soil C and N mineralization during secondary succession of subalpine forests.

The soil micro-food web is an important network of belowground trophic relationships and it participates directly and indirectly in soil ecological processes. In recent decades, the roles of the soil micro-food web in regulating ecosystem functions in grasslands and agroecosystems have received much attention. However, the variations in the soil micro-food web structure and its relationship with ecosystem functions during forest secondary succession remain unclear. In this study, we investigated how forest secondary succession affected the soil micro-food web (including soil microbes and nematodes) and soil carbon and nitrogen mineralization across a successional sequence of "grasslands - shrublands - broadleaf forests - coniferous forests" in a subalpine region of southwestern China. With forest successional development, the total soil microbial biomass and the biomass of each microbial group generally increased. The significant influences of forest succession on soil nematodes were mainly reflected in several trophic groups with high colonizer-persister values (particularly bacterivore3, herbivore5 and omnivore-predator5) that are sensitive to environmental disturbance. The increases in the connectance and nematode genus richness, diversity, and maturity index indicated an increasingly stable and complex soil micro-food web with forest succession, which was closely related to soil nutrients, particularly the soil carbon contents. Additionally, we found that the soil carbon and nitrogen mineralization rates also exhibited generally increasing trends during forest succession, which had significant positive correlations with the soil micro-food web composition and structure. The path analysis results indicated that the variances in ecosystem functions induced by forest succession were significantly determined by soil nutrients and soil microbial and nematode communities. Overall, these results suggested that forest succession enriched and stabilized the soil micro-food web and promoted ecosystem functions via the increase in soil nutrients, and the soil micro-food web played an important role in regulating ecosystem functions during forest succession.

pH-Dependent Changes in Structural Stabilities of Bt Cry1Ac Toxin and Contrasting Model Proteins following Adsorption on Montmorillonite.

The environmental fate of insecticidal Cry proteins, including time-dependent conservation of biological properties, results from their structural stability in soils. The complex cascade of reactions involved in biological action requires Cry proteins to be in solution. However, the pH-dependent changes in conformational stability and the adsorption-desorption mechanisms of Cry protein on soil minerals remain unclear. We used Derjaguin-Landau-Verwey-Overbeek (DLVO) calculation and differential scanning calorimetry to interpret the driving forces and structural stabilities of Cry1Ac and two contrasting model proteins adsorbed by montmorillonite. The structural stability of Cry1Ac is closer to that of the "hard" protein, α-chymotrypsin, than that of the "soft" bovine serum albumin (BSA). The pH-dependent adsorption of Cry1Ac and α-chymotrypsin could be explained by DLVO theory, whereas the BSA adsorption deviated from it. Patch-controlled electrostatic attraction, hydrophobic effects, and entropy changes following protein unfolding on a mineral surface could contribute to Cry1Ac adsorption. Cry1Ac, like chymotrypsin, was partly denatured on montmorillonite, and its structural stability decreased with an increase in pH. Moreover, small changes in the conformational heterogeneity of both Cry1Ac and chymotrypsin were observed following adsorption. Conversely, adsorbed BSA was completely denatured regardless of the solution pH. The moderate conformational rearrangement of adsorbed Cry1Ac may partially explain why the insecticidal activity of Bt toxin appears to be conserved in soils, albeit for a relatively short time period.

Functional shifts in soil fungal communities regulate differential tree species establishment during subalpine forest succession.

Soil fungi can differentially affect plant performance and community dynamics. While fungi play key roles in driving the plant-soil feedbacks (PSFs) that promote grassland succession, it remains unclear how the fungi-mediated PSFs affect tree species establishment during forest succession. We inoculated pioneer broadleaf (Betula platyphylla and Betula albosinensis) and nonpioneer coniferous tree seedlings (Picea asperata and Abies faxoniana) with fungal-dominated rooting zone soils collected from dominant plant species of early-, mid- and late-successional stages in a subalpine forest, and compared their biomass and fungal communities. All tree species accumulated abundant pathogenic fungi in early-successional inoculated soil, which generated negative biotic feedbacks and lowered seedling biomass. High levels of soil ectomycorrhizal fungi from mid- and late-successional stages resulted in positive biotic PSFs and strongly facilitated slow-growing coniferous seedling performance to favour successional development. B. albosinensis also grew better in mid- and late-successional soils with fewer pathogenic fungi than in early-successional soil, indicating its large susceptibility to pathogen attack. In contrast, the growth of another pioneer tree, B. platyphylla, was significantly suppressed in late-successional soil and was mostly driven by saprotrophic fungi, despite the unchanged pathogenic fungal community traits between the two fast-growing species. This unexpected result suggested a host specificity-dependent mechanism involved in the different impacts of fungal pathogens on host trees. Our findings reveal a critical role of functional shifts in soil fungal communities in mediating differential PSFs of tree species across successional stages, which should be considered to improve the prediction and management of community development following forest disturbances.

[Changes in the structure and function of soil prokaryotic communities in subalpine Picea asperata plantations]. / äºšé«˜å±±ç²—æžäº‘æ‰äººå·¥æž—åœŸå£¤åŽŸæ ¸å¾®ç”Ÿç‰©ç¾¤è½ç»“æž„ä¸ŽåŠŸèƒ½å˜åŒ–.

The structural and functional characteristics of soil prokaryotic community are important for maintaining ecosystem functions. In this study, we examined the diversity and compositions, the key drivers, as well as functional characteristics of prokaryotic communities in the rhizosphere and non-rhizosphere soils of Picea asperata with different stand ages using high-throughput sequencing technique and bioinformatics methods. The results showed that ß-diversity of soil prokaryotic communities in both rhizosphere and non-rhizosphere showed significant differences among different stand ages, but no significant difference between rhizosphere and non-rhizosphere in the same stand age. In terms of community composition at the phylum level, the relative abundances of Proteobacteria and Rokubacteria showed an increasing trend with the increases of stand age, while the relative abundance of Actinobacteria showed a decreasing trend, but no significant difference was observed between 75 year-old planted forests (PF75) and natural forests (NF). The relative abundances of Firmicutes and Thaumarchaeota in the soil of the 25 year-old planted forests (PF25) were significantly higher than in other planted forests and NF. At the genus level, the relative abundances of RB41, Terrimonas and Acidibacter showed an increasing trend with the increases of stand age, and RB41 and Terrimonas in rhizosphere soil of PF75 were significantly higher than those in NF. Soil properties and vegetation characteristics jointly influenced the structure of soil prokaryotic communities, with herb layer coverage, soil pH, total phosphorus, and total nitrogen as major drivers. The functional characteristics of soil prokaryotic communities were significantly different among different stand ages. The relative abundances of functions involved in carbon and nitrogen cycle, e.g., cellulolysis and nitrification, decreased with the increases of stand age, whereas that of sulfate respiration involved in the sulfur cycle increased. We proposed that the structure and functional characteristics of soil prokaryotic communities could serve as important indicators of the development stages of P. asperata forests. In the later stages of plantation forest development, soil nutrient availability could be improved by mediating phosphorus-dissolving and nitrogen-enhancing microorganisms to maintain the stability of the plantation ecosystem.

The rhizosphere effect of native legume Albizzia julibrissin on coastal saline soil nutrient availability, microbial modulation, and aggregate formation.

In the coastal zones, numerous ecological shelterbelt projects were conducted to protect against natural hazards. However, it is still not fully understood whether phytoremediation with native legume Albizzia julibrissin plantation can improve saline soil structural development or microbial community structure. In this study, a field experiment was conducted to investigate the responses of rhizosphere soil salinity, nutrients, bacterial community, and aggregate structure to A. julibrissin plantation in a recently reclaimed area along Zhejiang coast, China. After ~3-year plantation, rhizosphere soil pH and EC reduced to 8.25 and 0.14 dS·m-1, respectively, belonging to non-saline soil. Meanwhile, total organic carbon (TOC), permanganate-oxidizable carbon (POXC), total nitrogen (TN), alkali-hydrolyzable nitrogen (AN), and ammonium nitrogen (NH4+-N) were significantly increased in rhizosphere soil compared with bare land (P < 0.05). Consequently, rhizosphere soil had favorable habitat condition for copiotrophic bacterial taxa (e.g., Chloroflexi, Acidobacteria, and Bacteroidates), as well as high diversity, complex co-occurrence network, and catabolism related with nutrient cycling. The soil particle size of bare land was < 0.053 mm, while microaggregate (0.053-0.25 mm) and macroaggregate (0.25-2 mm) were formed in the rhizosphere and coupled with C accumulation and Fe removal. Soil aggregates were of great importance to soil fertility with more efficient bacterial network and biogeochemical cycles of nutrients. N-fixing Rhizobiales preferred to inhabit large soil particle and might primarily contribute to N accumulation. Generally, A. julibrissin was a suitable pioneer tree for mudflat reclamation projects, which effectively improved saline soil rhizosphere environment by reducing salinity, accumulating C and N, and promoting microbial community succession, as well as aggregate structure formation.

[Diversity and network features of fungal community in the soils of planted and natural Picea asperata forests.] / äººå·¥ä¸Žå¤©ç„¶äº‘æ‰æž—åœŸå£¤çœŸèŒç¾¤è½å¤šæ ·æ€§åŠèŒç¾¤ç½‘ç»œå ³ç³»ç‰¹å¾.

The diversity and interactions of soil fungal community are the key to maintain the diversity and stability of ecosystem. In this study, we examined the structure, diversity and co-occurrence networks of fungal community in rhizosphere and non-rhizosphere soils of planted and natural Picea asperata forests using high-throughput sequencing technique and bioinformatic methods. The results showed that Inocybaceae and Sebacinaceae were dominant family in soils of planted and natural forests, respectively. At the genus level, Inocybe was dominant one in soils of planted and natural forests. There were significant differences in ß-diversity of fungal communities between rhizosphere and non-rhizosphere soils in both planted and natural forests. There were no significant correlations between environmental variables and the relative abundance and α-diversity of fungal communities. Herb layer coverage, soil water content, total organic carbon concentration, and plant species richness played important roles in explaining the variations of ß-diversity of fungal communities. Results of the network analysis showed that the negative correlations were dominant among soil fungal communities in natural forest, suggesting that the competition of different groups in natural forest. Moreover, there were more negative correlations in non-rhizosphere soils than in rhizosphere soils, which indicated that fungal communities in non-rhizosphere soils comprised more competitive network structure than in the rhizosphere soils. Biomarker species were identified based on differential abundance analysis. Sebacinaceae was the single shared keystone species in the fungal network which had significant differences among rhizosphere and non-rhizosphere soils of planted and natural forests. Therefore, it is suggested that the variation of differential species in the soil fungal communities between the planted and natural forest might had limited influence on the stability of the community of planted and natural forests.

[Responses of polyphenoloxidase and catalase activities of rhizosphere and bulk soils to warming during the growing season in an alpine scrub ecosystem.] / é«˜å¯’çŒä¸›ç”Ÿé•¿å­£æ ¹é™ å’Œéžæ ¹é™ åœŸå£¤å¤šé šæ°§åŒ–é ¶å’Œè¿‡æ°§åŒ–æ°¢é ¶æ´»æ€§å¯¹å¢žæ¸©çš„å“åº”.

To understand the effects of climate warming on rhizosphere ecological processes in the alpine scrub ecosystem, the responses of polyphenoloxidase and catalase activities in the rhizosphere and bulk soils to experimental warming (1.3 ℃) were examined during the growing season in a Sibiraea angustata scrub ecosystem on the eastern Qinghai-Tibetan Plateau, China. The results showed that the activities of polyphenoloxidase in rhizosphere and bulk soils in the middle growing season were significantly higher than those in the early or late growing season. The activities of catalase in the bulk soil increased gradually during the growing season, while they showed no seasonal changes in the rhizosphere soil. In the bulk soil, warming significantly increased the activity of polyphenoloxidase by 17.5% in the late growing season and increased that of catalase by 2.2% in the middle growing season, whereas it did not affect soil enzyme activities in early or late growing seasons. In the rhizosphere soil, warming only significantly increased the activities of polyphenoloxidase and catalase by 6.5% and 1.3% in the early growing season. The rhizosphere effect of soil polyphenoloxidase activity was positive throughout the growing season, while there was no obvious rhizosphere effect for soil catalase activity. Furthermore, warming significantly decreased the rhizosphere effect of soil polyphenoloxidase activity by 15.2% during the late growing season. These results indicated that the activities of polyphenoloxidase and catalase activities differed between rhizosphere and bulk soils, with consequences on the rhizosphere soil ecological processes under climate warming in the alpine scrub ecosystem on the eastern Qinghai-Tibetan Plateau.

Responses of soil respiration and its components to experimental warming in an alpine scrub ecosystem on the eastern Qinghai-Tibet Plateau.

The responses of soil respiration (Rs) to warming are driven by its components, that is, heterotrophic respiration (Rh) and rhizosphere respiration (Rr, including fine root respiration [Rfr] and rhizomicrobial respiration [Rz]). However, the effects of warming on Rs and its components are rarely studied in alpine scrub ecosystems on the Qinghai-Tibet Plateau. Here, we conducted a warming experiment to examine how Rs and its components respond to experimental warming. We found that one-year experimental warming (increased by 1.3 °C) significantly stimulated the rates of Rs, Rh and Rr by 21.6, 23.8, and 21.1%, respectively. The annual cumulative C effluxes of Rs increased by 87.87 g m-2 with nearly equal contributions from Rh (46.84 g m-2) and Rr (41.03 g m-2) under warming. Moreover, warming-induced increase in Rr mainly resulted from increased Rfr rather than Rz due to the significant increase in fine root biomass and Rfr rate. In addition, the Q10 values for Rs, Rh and Rr were also increased by experimental warming, while the contribution of Rh to Rs was not altered by experimental warming. Collectively, our results suggest that future climatic warming will stimulate more C releases from soil to the atmosphere through an enhancement of both Rh and Rr in these alpine scrub ecosystems on the Qinghai-Tibet Plateau.

Plants regulate the effects of experimental warming on the soil microbial community in an alpine scrub ecosystem.

Information on how soil microbial communities respond to warming is still scarce for alpine scrub ecosystems. We conducted a field experiment with two plant treatments (plant removal or undisturbed) subjected to warmed or unwarmed conditions to examine the effects of warming and plant removal on soil microbial community structures during the growing season in a Sibiraea angustata scrubland of the eastern Qinghai-Tibetan Plateau. The results indicate that experimental warming significantly influenced soil microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN), but the warming effects were dependent on the plant treatments and sampling seasons. In the plant-removal plots, warming did not affect most of the microbial variables, while in the undisturbed plots, warming significantly increased the abundances of actinomycete and Gram-positive bacterial groups during the mid-growing season (July), but it did not affect the fungi groups. Plant removal significantly reduced fungal abundance throughout the growing season and significantly altered the soil microbial community structure in July. The interaction between warming and plant removal significantly influenced the soil MBC and MBN and the abundances of total microbes, bacteria and actinomycete throughout the growing season. Experimental warming significantly reduced the abundance of rare taxa, while the interaction between warming and plant removal tended to have strong effects on the abundant taxa. These findings suggest that the responses of soil microbial communities to warming are regulated by plant communities. These results provide new insights into how soil microbial community structure responds to climatic warming in alpine scrub ecosystems.

Bacterial cell surface properties: role of loosely bound extracellular polymeric substances (LB-EPS).

This study investigated the effect of loosely bound extracellular polymeric substances (LB-EPS) on the comprehensive surface properties of four bacteria (Bacillus subtilis, Streptococcus suis, Escherichia coli and Pseudomonas putida). The removal of LB-EPS from bacterial surfaces by high-speed centrifugation (12,000×g) was confirmed by SEM images. Viability tests showed that the percentages of viable cells ranged from 95.9% to 98.0%, and no significant difference was found after treatment (P>0.05). FTIR spectra revealed the presence of phosphodiester, carboxylic, phosphate, and amino functional groups on bacteria surfaces, and the removal of LB-EPS did not alter the types of cell surface functional groups. Potentiometric titration results suggested the total site concentrations on the intact bacteria were higher than those on LB-EPS free bacteria. Most of the acidity constants (pKa) were almost identical, except the increased pKa values of phosphodiester groups on LB-EPS free S. suis and E. coli surfaces. The electrophoretic mobilities and hydrodynamic diameters of the intact and LB-EPS free bacteria were statistically unchanged (P>0.05), indicating LB-EPS had no influence on the net surface charges and size distribution of bacteria. However, LB-ESP could enhance cell aggregation processes. The four LB-EPS free bacteria all exhibited fewer hydrophobicity values (26.1-65.0%) as compared to the intact cells (47.4-69.3%), suggesting the removal of uncharged nonpolar compounds (e.g., carbohydrates) in LB-EPS. These findings improve our understanding of the changes in cell surface characterizations induced by LB-EPS, and have important implications for assessing the role of LB-EPS in bacterial adhesion and transport behaviors.

Streptococcus suis sorption on agricultural soils: role of soil physico-chemical properties.

Understanding pathogen sorption on natural soil particles is crucial to protect public health from soilborne and waterborne diseases. Sorption of pathogen Streptococcus suis on 10 agricultural soils was examined, and its correlations with soil physico-chemical properties were also elucidated. S. suis sorption isotherms conformed to the linear equation, with partition coefficients (Ks) ranging from 12.7 mL g(-1) to 100.1 mL g(-1). Bacteria were observed to sorb on the external surfaces of soil aggregates by scanning electron microscopy. Using Pearson correlation and linear regression analysis, solution pH was found to have significant negative correlations with Ks. Stepwise multiple regression and path analysis revealed that pH and cation exchange capacity (CEC) were the main factors influencing sorption behaviors. The obtained overall model (Ks=389.6-45.9×pH-1.3×CEC, R(2)=0.943, P<0.001) can accurately predict Ks values. However, the variability in Ks was less dependent on soil organic matter, specific surface area, soil texture and zeta potential, probably due to the internal-surface shielding phenomenon of soil aggregates. Additionally, the sorption trends cannot be interpreted by interaction energy barriers calculated using the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, suggesting the limits of DLVO theory in describing pathogen sorption on natural soils. Our results also indicated soil pH and CEC should be preferentially considered when modeling S. suis sorption process.

Adhesion of bacterial pathogens to soil colloidal particles: influences of cell type, natural organic matter, and solution chemistry.

Bacterial adhesion to granular soil particles is well studied; however, pathogen interactions with naturally occurring colloidal particles (<2 µm) in soil has not been investigated. This study was developed to identify the interaction mechanisms between model bacterial pathogens and soil colloids as a function of cell type, natural organic matter (NOM), and solution chemistry. Specifically, batch adhesion experiments were conducted using NOM-present, NOM-stripped soil colloids, Streptococcus suis SC05 and Escherichia coli WH09 over a wide range of solution pH (4.0-9.0) and ionic strength (IS, 1-100 mM KCl). Cell characterization techniques, Freundlich isotherm, and Derjaguin-Landau-Verwey-Overbeek (DLVO) theory (sphere-sphere model) were utilized to quantitatively determine the interactions between cells and colloids. The adhesion coefficients (Kf) of S. suis SC05 to NOM-present and NOM-stripped soil colloids were significantly higher than E. coli WH09, respectively. Similarly, Kf values of S. suis SC05 and E. coli WH09 adhesion to NOM-stripped soil colloids were greater than those colloids with NOM-present, respectively, suggesting NOM inhibits bacterial adhesion. Cell adhesion to soil colloids declined with increasing pH and enhanced with rising IS (1-50 mM). Interaction energy calculations indicate these adhesion trends can be explained by DLVO-type forces, with S. suis SC05 and E. coli WH09 being weakly adhered in shallow secondary energy minima via polymer bridging and charge heterogeneity. S. suis SC05 adhesion decreased at higher IS 100 mM, which is attributed to the change of hydrophobic effect and steric repulsion resulted from the greater presence of extracellular polymeric substances (EPS) on S. suis SC05 surface as compared to E. coli WH09. Hence, pathogen adhesion to the colloidal material is determined by a combination of DLVO, charge heterogeneity, hydrophobic and polymer interactions as a function of solution chemistry.