Replacing traditional nursery soil with spent mushroom substrate improves rice seedling quality and soil substrate properties.

Currently, large quantities of spent mushroom substrate (SMS) are produced annually. Because SMS has high water retention and nutrients, it has great potential to replace traditional topsoil for raising seedlings in agricultural production. However, few studies have examined the effects of substituting SMS for paddy soil on rice seedling growth and soil nutrients. SMS was mixed with rice soil in different proportions (20%, 50%, and 80%), and chemical fertilizer, organic fertilizer, and peat substrate were added in addition to equivalent nitrogen as a traditional seedling nursery method for comparison. Compared to traditional paddy soil (CK), the seedling qualities of the three SMS ratio treatments were all higher. Adding SMS at different ratios promoted rice seedling root growth, elevated the soluble protein concentration, and amplified the superoxide dismutase (SOD) enzymatic action in rice seedlings. Total porosity and aeration porosity of the soil increased by 17.40% and 32.90%, respectively. Soil organic carbon (SOC), total nitrogen (TN), and total phosphorus (TP) increased by 21.26-118.48%, 50.44-71.68%, and 23.08-80.17%, respectively. Besides, the relative abundance of Bacillus, Bacteroidetes, and other bacteria as well as the abundance of Ascomycota were all significantly increased. Adding 50% SMS increased the abundance of Pseudomonas by 8.42 times. The seedling quality of the 50% SMS treatment was even higher than chemical fertilizer and organic fertilizer treatments, only second to the peat substrate treatment. In summary, partial substitution of paddy soil with SMS can ameliorate substrate properties, improve seedling quality, and increase microbial diversity, indicating the suitability of SMS as a replacement for rice soil in seedling substrates. The 50% SMS ratio is the best. This study provides a basis for SMS to replace traditional rice soil in seedling cultivation.

Recent advances on environmental behavior of Cu-based nanomaterials in soil-plant system: A review.

In recent years, copper-based nanomaterials (Cu-based NMs) have shown great potential in promoting agriculture development due to their special physicochemical characteristics. With the mass production and overuse of Cu-based NMs, there are potential effects on the soil-plant environment. Soil organisms, especially soil microorganisms, play a significant part in terrestrial or soil ecosystems; plants, as indirect organisms with soil-related Cu-based NMs, may affect human health through plant agricultural products. Understanding the accumulation and transformation of Cu-based NMs in soil-plant systems, as well as their ecotoxicological effects and potential mechanisms, is a prerequisite for the scientific assessment of environmental risks and safe application. Therefore, based on the current literature, this review: (i) introduces the accumulation and transformation behaviors of Cu-based NMs in soil and plant systems; (ii) focuses on the ecotoxicological effects of Cu-based NMs on a variety of organisms (microorganisms, invertebrates, and plants); (iii) reveals their corresponding toxicity mechanisms. It appears from studies hitherto made that both Cu-based NMs and released Cu2+ may be the main reasons for toxicity. When Cu-based NMs enter the soil-plant environment, their intrinsic physicochemical properties, along with various environmental factors, could also affect their transport, transformation, and biotoxicity. Therefore, we should push for intensifying the multi-approach research that focuses on the behaviors of Cu-based NMs in terrestrial exposure environments, and mitigates their toxicity to ensure the promotion of Cu-based NMs.

Electro-Fenton degradation of pefloxacin using MOFs derived Cu, N co-doped carbon as a nanocomposite catalyst.

Electro-Fenton (EF) can in-situ produce H2O2 and effectively activate H2O2 to generate powerful reactive species for the destruction of contaminants under acidic conditions, however, the production of iron-containing sludge and requirement of low working pH significantly hinder its practical application. Herein, a novel Cu, N co-doped carbon (Cu-N@C) with metal organic framework (MOF) as a precursor was constructed and adopted for the elimination of pefloxacin (PEF) in the heterogeneous electro-Fenton (HEF) process. PEF could be almost completely removed within 1 h and total organic carbon (TOC) removal efficiency was 48.57% within 6 h. Meanwhile, Cu-N@C had good repeatability and environmental adaptability, it can still maintain excellent catalytic performance after 10 cycles, and it exhibited satisfactory remediation performance in simulated water matrix. In addition, the HEF process catalyzed by Cu-N@C also showed satisfactory degradation effect on other organic pollutants including atrazine, methylene blue, and chlorotetracycline. Under the action of impressed current, the HEF system could generate H2O2 in-situ, and the active species could be generated in the redox cycle of Cu0/Cu1+/Cu2+. Electron paramagnetic resonance and quenching experiments confirmed that •OH was the dominant active species in the degradation of organic compounds. The degradation process of PEF was studied by mass spectrometry analysis of intermediate products. This study provided a simple method to prepare MOF-based electrocatalyst, which exhibits promising application potential for treatment wastewater.

Effect of various phosphorus levels on the extraction of Cd, the transformation of P, and phosphorus-related gene during the phytoremediation of Cd contaminated soil.

Phytoremediation has emerged as a common technique for remediating Cd pollution in farmland soil. Moreover, phosphorus, an essential element for plants, can alter the pectin content of plant cell walls and facilitate the accumulation of Cd in plant tissues, thereby enhancing phytoremediation efficiency. Therefore, pot experiments were conducted in order to investigate the effect of phosphorus levels on Cd extraction, phosphorus transformation and phosphorus-related genes during phytoremediation. The results revealed that an optimal application of suitable phosphate fertilizers elevated the soil's pH and electrical conductivity (EC), facilitated the conversion of soil from insoluble phosphorus into available forms, augmented the release of pertinent enzyme activity, and induced the expression of phosphorus cycling-related genes. These enhancements in soil conditions significantly promoted the growth of ryegrass. When applying phosphorus at a rate of 600 mg/kg, ryegrass exhibited plant height, dry weight, and chlorophyll relative content that were 1.27, 1.26, and 1.18 times higher than those in the control group (P0), while the Cd content was 1.12 times greater than that of P0. The potentially toxic elements decline ratio and bioconcentration factor were 42.86% and 1.17 times higher than those of P0, respectively. Consequently, ryegrass demonstrated the highest Cd removal efficiency under these conditions. Results from redundancy analysis (RDA) revealed a significant correlation among pH, total phosphorus, heavy metal content, phosphorus forms, soil enzyme activity, and phosphorus-related genes. In conclusion, this study suggests applying an optimal amount of suitable phosphate fertilizers can enhance restoration efficiency, leading to a reduction in soil Cd content and ultimately improving the safety of crop production in farmlands.

Occurrence, bioaccumulation, fate, and risk assessment of emerging pollutants in aquatic environments: A review.

Significant concerns on a global scale have been raised in response to the potential adverse impacts of emerging pollutants (EPs) on aquatic creatures. We have carefully reviewed relevant research over the past 10 years. The study focuses on five typical EPs: pharmaceuticals and personal care products (PPCPs), per- and polyfluoroalkyl substances (PFASs), drinking water disinfection byproducts (DBPs), brominated flame retardants (BFRs), and microplastics (MPs). The presence of EPs in the global aquatic environment is source-dependent, with wastewater treatment plants being the main source of EPs. Multiple studies have consistently shown that the final destination of most EPs in the water environment is sludge and sediment. Simultaneously, a number of EPs, such as PFASs, MPs, and BFRs, have long-term environmental transport potential. Some EPs exhibit notable tendencies towards bioaccumulation and biomagnification, while others pose challenges in terms of their degradation within both biological and abiotic treatment processes. The results showed that, in most cases, the ecological risk of EPs in aquatic environments was low, possibly due to potential dilution and degradation. Future research topics should include adding EPs detection items for the aquatic environment, combining pollution, and updating prediction models.

Current progress on manganese in constructed wetlands: Bibliometrics, effects on wastewater treatment, and plant uptake.

Constructed wetlands (CWs) are a pollutant treatment design inspired by natural wetlands and are widely utilized for the removal of common pollutants. The research focus lies in the circulation of manganese (Mn) in the environment to enhance pollutant removal within CWs. This paper provides a comprehensive review of recent advancements in understanding the role and effects of Mn in chemical weapons, based on literature retrieval from 2002 to 2021. Ecological risk assessment and heavy metals within CWs emerge as current areas of research interest. Mn sources within CWs primarily include natural deposition, heavy metal wastewater, and intentional addition. The cycling between Mn(II) and Mn(IV) facilitates enhanced wastewater treatment within CWs. Moreover, employing a Mn matrix proves effective in reducing ammonia nitrogen wastewater, organic pollutants, as well as heavy metals such as Cd and Pb, thereby addressing complex pollution challenges practically. To comprehensively analyze influencing factors on the system's performance, both internal factors (biological species, design parameters, pH levels, etc.) and external factors (seasonal climate variations, precipitation patterns, ultraviolet radiation exposure, etc.) were discussed. Among these factors, microorganisms, pollutants, and temperature are the most important influencing factors, which emphasizes the importance of these factors for wetland operation. Lastly, this paper delves into plant absorption of Mn along with coping strategies employed by plants when faced with Mn poisoning or deficiency scenarios. When utilizing Mn for the regulation of constructed wetlands, it is crucial to consider the tolerance levels of associated plant species. Furthermore, the study predicts future research hotspots encompass high-efficiency catalysis techniques, matrix-filling approaches, and preparation of resource utilization methods involving Mn nanomaterials.

Metal-Organic Frameworks Based Sensor Platforms for Rapid Detection of Contaminants in Wastewater.

Metal-organic frameworks (MOFs) are a type of multifunctional material with organic-inorganic doped metal complexes that have a lot of unsaturated metal sites and a consistent network structure. MOFs work has great performance for enhancing the mass transfer, signal, and sensitivity as well as analyte enrichment. This study highlights the recent advancements of MOFs-based sensors for pollutant detection in a water environment and summarizes the effect of various synthetic materials on the performance of MOFs-based sensors. The related challenges and optimization techniques have been discussed. Then the research results of various MOFs sensors in the detection of wastewater pollutants are analyzed. Finally, the challenges facing MOFs-based water sensor development and the outlook for future research are discussed.

Fundamentals and application in phytoremediation of an efficient arsenate reducing bacterium Pseudomonas putida ARS1.

Arsenic is a ubiquitous environmental pollutant. Microbe-mediated arsenic bio-transformations significantly influence arsenic mobility and toxicity. Arsenic transformations by soil and aquatic organisms have been well documented, while little is known regarding effects due to endophytic bacteria. An endophyte Pseudomonas putida ARS1 was isolated from rice grown in arsenic contaminated soil. P. putida ARS1 shows high tolerance to arsenite (As(III)) and arsenate (As(V)), and exhibits efficient As(V) reduction and As(III) efflux activities. When exposed to 0.6 mg/L As(V), As(V) in the medium was completely converted to As(III) by P. putida ARS1 within 4 hr. Genome sequencing showed that P. putida ARS1 has two chromosomal arsenic resistance gene clusters (arsRCBH) that contribute to efficient As(V) reduction and As(III) efflux, and result in high resistance to arsenicals. Wolffia globosa is a strong arsenic accumulator with high potential for arsenic phytoremediation, which takes up As(III) more efficiently than As(V). Co-culture of P. putida ARS1 and W. globosa enhanced arsenic accumulation in W. globosa by 69%, and resulted in 91% removal of arsenic (at initial concentration of 0.6 mg/L As(V)) from water within 3 days. This study provides a promising strategy for in situ arsenic phytoremediation through the cooperation of plant and endophytic bacterium.

Critical review on organophosphate esters in water environment: Occurrence, health hazards and removal technologies.

Organophosphate esters (OPEs), which are phosphoric acid ester derivatives, are anthropogenic substances that are widely used in commerce. Nevertheless, there is growing public concern about these ubiquitous contaminants, which are frequently detected in contaminated water sources. OPEs are mostly emitted by industrial operations, and the primary routes of human exposure to OPEs include food intake and dermal absorption. Because of their negative effects on both human health and the environment, it is clear that innovative methods are needed to facilitate their eradication. In this study, we present a comprehensive overview of the existing characteristics and origins of OPEs, their possible impacts on human health, and the merits, drawbacks, and future possibilities of contemporary sophisticated remediation methods. Current advanced remediation approaches for OPEs include adsorption, degradation (advanced oxidation, advanced reduction, and redox technology), membrane filtration, and municipal wastewater treatment plants, degradation and adsorption are the most promising removal technologies. Meanwhile, we proposed potential areas for future research (appropriate management approaches, exploring the combination treatment process, economic factors, and potential for secondary pollution). Collectively, this work gives a comprehensive understanding of OPEs, providing useful insights for future research on OPEs pollution.

Degradation of sulfanilamide in aqueous solution by ionizing radiation: Performance and mechanism.

Sulfonamide (SA) is an emerging contaminants and the efficient treatment of SA containing wastewater remains a challenge. Herein, SA degradation by gamma irradiation has been systematacially studied. SA (10 mg/L) could be totally removed with 1.5 kGy irradiation. Quenching experiments demonstrated that •OH and eaq- were the predominant for SA degradation. SA degradation was reduced with initial concentration increasing, and the removal was faster with pH increasing in the range of 3.1-10.8. The coexisting matters affected SA degradation through changing reactive species, and the introduction of SO42- and Cl- enhanced SA degradation, while CO32- had a negative impact on SA degradation, and the degradation was insignificantly affected when adding humic acid. Gamma irradiation could remain effective in real water matrixes. In conjunction with LC-MS analysis and DFT calculation, possible degradation pathways for SA were proposed. Gamma irradiation could reduce the toxicity of SA, while several byproducts with more toxic were also formed. Furthermore, gamma/priodate (PI) process was promising to enhance SA degradation and mineralization. k value increased by 1.85 times, and mineralization rate increased from 19.51% to 79.19% when adding PI. This study suggested that ionizing radiation was efficient to eliminate SA in wastewater.

Current progress on fluoride occurrence in the soil environment: Sources, transformation, regulations and remediation.

Fluorine is a halogen element widely distributed in nature, but due to excessive emissions from industrial manufacturing and agricultural production, etc., the soil is over-enriched with fluoride and the normal growth of plants is under stress, and it also poses a great threat to human health. In this review, we summarized the sources of fluoride in soil, and then analyzed the potential mechanisms of fluoride uptake in soil-plant systems. In addition, the main influences of soil ecosystems on plant fluoride uptake were discussed, soil management options to mitigate fluoride accumulation in plants were also summarized. The bioremediation techniques were found to be a developmental direction to improve fluoride pollution. Finally, we proposed other research directions, including fluoride uptake mechanisms in soil-plant systems at the molecular expression levels, development of visualization techniques for fluoride transport in plants, interactions mechanisms between soil microhabitats and plant metabolism affecting fluoride uptake, as well as combining abiotic additives, nanotechnology and biotechnology to remediate fluoride contamination problems.

Reductive soil disinfestation and Fe amendment improve soil microbial composition and Fritillaria production.

The continuous obstacles of cropping cause severe economic loss, which seriously threaten agricultural sustainable development. In addition, managing excess waste, such as potato peel and mineral waste residues, is a vital burden for industry and agriculture. Therefore, we explored the feasibility of reductive soil disinfestation (RSD) with potato peel and amendment with iron mineral waste residues for the production of Fritillaria thunbergii, which is vulnerable to continuous obstacles. In this study, the influences of iron mineral, RSD with different organic maters, as well as the combined effects of iron mineral and RSD on Fritillaria rhizosphere soil physicochemical properties, microbial communities, and Fritillaria production were investigated. The results revealed that the RSD treatments with potato peel significantly reduced the soil salinity and increased the soil pH, microbial activity, organic matter, and the contents of K and Ca. RSD with potato peel also significantly thrived of the beneficial microbes (Bacillus, Azotobacter, Microvirga, and Chaetomium), and down-regulated potential plant pathogens. RSD with potato peel significantly promoted F. thunbergii yield and quality. Moreover, the combined effects of RSD and iron mineral amendment further enhanced soil health, improved microbial community composition, and increased the yield and peimisine content of F. thunbergii by 24.2% and 49.3%, respectively. Overall, our results demonstrated that RSD with potato peel and amendment with iron mineral waste residues can efficiently improve soil fertility, modify the microbial community, and benefit for both the sustainable production of F. thunbergii and the management of waste. KEY POINTS: • RSD increases soil pH, organic matter, microbial activity, and mineral content • RSD with potato peel enriches beneficial microbes and decreases plant pathogens • PP + Fe treatment increases Fritillaria yield by 24.2% and peimisine content by 49.3.

Integrative application of biochar and arbuscular mycorrhizal fungi for enhanced chromium resistance in Medicago sativa.

Soil chromium (Cr) contamination has become an environmental problem of global concern. However, the joint effects of combined utilization of biochar and arbuscular mycorrhizal (AM) fungal inoculum, which are considered as two promising remediation strategies of soil heavy metal pollutions, on plant Cr resistance are still poorly understood. In this study, a two-factor pot experiment was conducted to investigate how biochar and AM fungus Rhizophagus irregularis regulate Medicago sativa growth, physiological trait, nutrient and Cr uptake, relevant gene expressions, soil properties, and Cr speciation, independently or synergistically. The results showed that biochar notably decreased AM colonization, while biochar and AM fungus could simultaneously increase plant dry biomass. The greatest growth promotion was observed in mycorrhizal shoots at the highest biochar level (50 g kg-1 soil) by 91 times. Both biochar application and AM fungal inoculation enhanced plant photosynthesis and P nutrition, but the promoting effects of AM fungus on them were significantly greater than that of biochar. In addition, the combined application of biochar and AM fungus dramatically reduced shoot and root Cr concentrations by up to 92 % and 78 %, respectively, compared to the non-amended treatment. Meanwhile, down-regulated expressions were observed for metal chelating-related genes. Furthermore, Cr translocation from roots to shoots was reduced by both two soil amendments. Transcriptional levels of genes involved in reactive oxygen species and proline metabolisms were also regulated by biochar application and AM fungal colonization, leading to alleviation of Cr phytotoxicity. Furthermore, AM fungal inoculation slightly elevated soil pH but decreased plant-available soil P, which was, by contrast, lifted by biochar addition. The combined application reduced soil acid-extractable Cr concentration by 40 %. This study provides new insights into comprehensively understanding of the mechanisms of biochar and AM fungi combination on improving plant Cr tolerance.

Cleaner production of Chinese cabbage by intercropping from Cd contaminated soil: Effects of hyperaccumulator variety and planting strip width.

The utilization of Cd-contaminated soil in vegetable crop production can lighten the food crisis and improve the soil environmental resilience. Intercropping is a reliable technology in safety production from contaminated soil. A field-scale experiment was carried out to unravel how plant species and pattern affect the growth and Cd uptake of Chinese cabbage from Cd contaminated land. Among all the intercropping systems designed in this study, one row of Chinese cabbage intercropping with one row of Solanum nigrum L. is the best planting mode (high yields (2.78 kg/m2) and low Cd accumulation (0.02 mg/kg) of Chinese cabbage). Combined with the in-depth joint analysis of diverse soil physicochemical features (soil nutrient characteristics and microbial community structure), biomass yield and quality, and soil microbiological properties, we elaborated that two measures (screening hyperaccumulation types and controlling planting strip width) were the major factors in determining the growth of the aboveground and underground parts of Chinese cabbage respectively, thus directly regulating the application effectiveness of intercropping technology. The intertwined mechanisms (interspecific and intraspecific relationship) of different intercropping systems are summarized, which include better utilization of space, light and other resources in the aboveground part, bioavailability of nutrient, drive of soil bacteria and alleviated soil Cd stress in the underground part, etc. Our research outputs indicate the effectiveness and feasibility of intercropping can be improved by optimizing the streamline configuration and plant mode, which provide theory of reference and practical evidence for warranting the food safety and agricultural soil remediation simultaneously.

Effect of the Combination of Phosphate-Solubilizing Bacteria with Orange Residue-Based Activator on the Phytoremediation of Cadmium by Ryegrass.

Amendments with activators or microorganisms to enhance phytoremediation in toxic-metal-polluted soils have been widely studied. In this research, the production of indoleacetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase by phosphate-solubilizing bacteria was investigated during a pure culture experiment. Pot experiments were performed using Cd-polluted soil with the following treatments: control (CK, only ultrapure water), orange-peel-based activator (OG), and a combination of phosphate-solubilizing bacteria (Acinetobacter pitti) and OG (APOG). Ryegrass plant height and fresh weight, Cd content in ryegrass, total and available Cd soil content, soil enzyme activity, and soil bacterial diversity were determined in this work. The findings showed that the height of ryegrass in OG and APOG increased by 14.78% and 21.23%. In the APOG group, a decreased ratio of Cd was 3.37 times that of CK, and the bioconcentration factor was 1.28 times that of CK. The neutral phosphatase activity of APOG was 1.33 times that of CK and catalase activity was 1.95 times that of CK. The activity of urease was increased by 35.48%. APOG increased the abundance of beneficial bacteria and Proteobacteria was the dominant bacterium, accounting for 57.38% in APOG. Redundancy analysis (RDA) showed that nutrient elements were conducive to the propagation of the dominant bacteria, the secretion of enzymes, and the extraction rate of Cd in the soil. The possible enhancement mechanism of phytoremediation of cadmium by A. pitti combined with OG was that, on the one hand, APOG increased soil nutrient elements and enzyme activities promoted the growth of ryegrass. On the other hand, APOG activated Cd and boosted the movement of Cd from soil to ryegrass. This research offers insight for the combination of phosphate-solubilizing bacteria with an orange-peel-based activator to improve phytoremediation of Cd-contaminated soils and also provides a new way for the resource utilization of fruit residue.

Bioavailability and translocation of platinum nanoparticles and platinum ions in rice (Oryza sativa L.): Nanoparticles biosynthesis and size-dependent transformation.

Metal nanoparticles accumulation and bioavailability in plants raised much attention, specifically transformation and transportation of nanoparticles and their corresponding ions in plants are still unknown. In this work, rice seedlings were exposed to platinum nanoparticles (PtNPs) (with three sizes of 25, 50, and 70 nm) and Pt ions (with doses of 1, 2, and 5 mg/L) to investigate the influences of particle size and Pt form on bioavailability and translocation mechanism of metal nanoparticles. Results based on single particle ICP-MS (SP-ICP-MS) demonstrated the biosynthesis of PtNPs in Pt ions treated rice seedlings. The particle size ranges at 75-79.3 nm were detected in Pt ions exposed rice roots, and further migrated up to rice shoots at 21.7-44.3 nm. After exposed to PtNP-25, the particles could transfer to shoots with the original size distribution detected in roots, even with the PtNPs dose change. PtNP-50 and PtNP-70 translocated to shoots with the particle size increase. For the rice exposure with three dose levels, PtNP-70 had the highest number-based bioconcentration factors (NBCFs) in all Pt species, while Pt ions had the highest bioconcentration factors (BCFs), a range of 1.43-2.04. All PtNPs and Pt ions could be accumulated in rice plants and further transferred to shoots, and particle biosynthesis was proved through SP-ICP-MS. The finding could help us better understand the influence of particle size and form on the transformations of PtNPs in environment.

Simultaneous adsorption of ciprofloxacin and Cu2+ using Fe and N co-doped biochar: Competition and selective separation.

The treatment of combined antibiotics and heavy metals pollution is a critical challenge. Herein, iron and nitrogen co-doped biochar (Fe/N-BC) was synthesized using rape straw as precursor, and applied for the adsorption of ciprofloxacin (CIP) and Cu2+ in single and binary systems. The qmax for CIP and Cu2+ were 46.45 mg g-1 and 30.77 mg g-1, respectively. Adsorption decreased in a binary matrix, indicating that there was a competitive effect between CIP and Cu2+, which might be due to CIP and Cu2+ sharing similar active adsorption sites on Fe/N-BC. Interestingly, CIP and Cu2+ co-adsorption was a pH-dependent process. Fe/N-BC has potential to highly selectively separate CIP/Cu2+ from mixed solutions through adjusting pH values. Furthermore, adsorption mechanisms were systematically investigated in this research. This research could help to provide a deeper understanding of the synchronously removing specific antibiotics and heavy metals by biochar adsorbents.

Detection and removal technologies for ammonium and antibiotics in agricultural wastewater: Recent advances and prospective.

With the extensive development of industrial livestock and poultry production, a considerable part of agricultural wastewater containing tremendous ammonium and antibiotics have been indiscriminately released into the aquatic systems, causing serious harms to ecosystem and human health. In this review, ammonium detection technologies, including spectroscopy and fluorescence methods, and sensors were systematically summarized. Antibiotics analysis methodologies were critically reviewed, including chromatographic methods coupled with mass spectrometry, electrochemical sensors, fluorescence sensors, and biosensors. Current progress in remediation methods for ammonium removal were discussed and analyzed, including chemical precipitation, breakpoint chlorination, air stripping, reverse osmosis, adsorption, advanced oxidation processes (AOPs), and biological methods. Antibiotics removal approaches were comprehensively reviewed, including physical, AOPs, and biological processes. Furthermore, the simultaneous removal strategies for ammonium and antibiotics were reviewed and discussed, including physical adsorption processes, AOPs, biological processes. Finally, research gaps and the future perspectives were discussed. Through conducting comprehensive review, future research priorities include: (1) to improve the stabilities and adaptabilities of detection and analysis techniques for ammonium and antibiotics, (2) to develop innovative, efficient, and low cost approaches for simultaneous removal of ammonium and antibiotics, and (3) to explore the underlying mechanisms that governs the simultaneous removal of ammonium and antibiotics. This review could facilitate the evolution of innovative and efficient technologies for ammonium and antibiotics treatment in agricultural wastewater.

Metagenomic insights into the inhibitory mechanisms of Cu on fermentative hydrogen production.

Cu is widely present in the feedstocks of dark fermentation, which can inhibit H2 production efficiency of the process. However, current understanding on the inhibitory mechanisms of Cu, especially the microbiological mechanism, is still lacking. This study investigated the inhibitory mechanisms of Cu2+ on fermentative hydrogen production by metagenomics sequencing. Results showed that the exposure to Cu2+ reduced the abundances of high-yielding hydrogen-producing genera (e.g. Clostridium sensu stricto), and remarkably down-regulated the genes involved in substrate membrane transport (e.g., gtsA, gtsB and gtsC), glycolysis (e.g. PK, ppgK and pgi-pmi), and hydrogen formation (e.g. pflA, fdoG, por and E1.12.7.2), leading to significant inhibition on the process performances. The H2 yield was reduced from 1.49 mol H2/mol-glucose to 0.59 and 0.05 mol H2/mol-glucose upon exposure to 500 and 1000 mg/L of Cu2+, respectively. High concentrations of Cu2+ also reduced the rate of H2 production and prolonged the H2-producing lag phase.

Soil science and environmental research.

As indicated in the call for papers posted for this Special Issue, Soil Science deals with various environmental compartments, so it is closely related to Environmental Research. It is clear that synergisms and collaboration are keys to reach the most fruitful relations among different sciences and scientists, and especially in all that focused on the Environment. In this line, considering Soil Science, Environmental Research, and the multiple and complex eventual combinations involving them, could give new highly interesting works focused on any of the specific subjects covered, as well as on relations among these sciences. The main objective should be going further in positive interactions that could help in protecting the Environment, proposing solutions to face hazards that are drastically threatening our planet. In view of that, the Editors of this Special Issue invited researchers to submit high-quality manuscripts including new experimental data, as well as scientifically founded discussion and reflections on the matter. The VSI has received 171 submissions, with 27% of them being accepted after peer-review. The Editors think that the papers included in this VSI have high scientific value and provide scientific knowledge on the field. In this editorial piece the Editors include comments and reflections on the papers published in the SI.