[Effect of invisible appliance without bracket and self-locking bracket appliance on periodontal and subgingival flora in patients with periodontitis].

PURPOSE: To observe the effect of non-bracket invisible appliance and self-locking bracket appliance on periodontal health and subgingival flora in patients with periodontitis. METHODS: One hundred and ten patients with periodontitis who received orthodontic treatment were divided into invisible group and self-locking bracket group. The periodontal index including probing depth (PD), gingival index (GI), sulcular bleeding index (SBI) and plaque index (PLI) ，and the levels of inflammatory factors in the gingival crevicular fluid were examined. Visual analogue scale (VAS) was used to evaluate periodontal pain before and after correction, and the curative effect was evaluated. The status of common pathogenic bacteria in the subgingival plaque of patients after correction was detected by PCR. The data were analyzed with SPSS19.0 software package. RESULTS: Twelve months after treatment and at the end of treatment, PLI of the self-locking bracket group was significantly higher than the invisible group(P＜0.05), but PD, GI and SBI between the two groups had no significant difference. After correction, the inflammatory factors in the invisible group were significantly lower than those in the self-locking bracket group(P＜0.05). At 1d after treatment, pain index in the invisible group was significantly lower than the self-locking group(P＜0.05). The clinical total effective rate of the invisible group was significantly higher than the self-locking group (89.10% vs 76.36%). After treatment, the detection rate and the content of subgingival pathogenic bacteria in the self-locking bracket group were significantly higher than the invisible group(P＜0.05). CONCLUSIONS: During orthodontic treatment of periodontitis, wearing non-bracket invisible appliance can effectively inhibit inflammatory response and proliferation of subgingival pathogens, which is more conducive to the maintenance of periodontal health and oral hygiene.

[Chemical fingerprinting and similarity analysis of Lonicera japonica resources].

LC-MS was used to detect 41 population of Lonicera japonica from different areas. LC-MS chemical chromatographic profile has been established. There were 23 common peaks, seventeen of which were identified according to reference standard and reference; SPSS software was applied to calculate the similarity of chemical fingerprints of 41 batches and the range was from 0.99 to 0.12. On this basis, the L. japonica's metabolites consistency was classified. Combined with comprehensive analysis of genetic identity, we can provide a theoretical basis for the authenticity research of Dao-di herbs and reference information for the breeding of excellent L. japonica.

[Establishment of DNA identity card and analysis of genetic similarity among 58 varieties in Lonicera japonica].

A total of 58 varieties in Lonicera japonica from 20 producing areas were amplified by 22 pairs of SSR primers. Seven pairs of polymorphic primers were screened and their primers were used to establish DNA identity card and analyze genetic similarity.All the 58 varieties could be distinguished each other by the DNA identity card constituted by 7 pairs of core SSR primers.The genetic similarity coefficients of 58 varieties ranged from 0.366 7 to 0.916 7 by using PopGene32（vesion1.32）. Furthermore, all the varieties consistency were classified into 4 groups and constructed an evaluation table according to cluster analysis by an un-weighted pair-group average method with arithmetic mean. As expected, the results of cluster and evaluation table reflected 58 varieties relatives, which provide reference information for the selection of fine germplasm of L. japonica and the theoretical basis for the study of Dao-di herbs.