Ecotoxicological assessment of UV filters benzophenone-3 and TiO2 nanoparticles, isolated and in a mixture, in developing zebrafish (Danio rerio).

The increasing use of UV filters, such as benzophenone-3 (BP-3) and titanium dioxide nanoparticles (TiO2 NPs), has raised concerns regarding their ecotoxicological effects on the aquatic environment. The aim of the present study was to examine the embryo-larval toxicity attributed to BP-3 or TiO2 NPs, either alone or in a mixture, utilizing zebrafish (Danio rerio) as a model after exposure to environmentally relevant concentrations of these compounds. Zebrafish embryos were exposed to BP-3 (10, 100, or 1000 ng/L) or TiO2 NPs (1000 ng/L) alone or in a mixture (BP-3 10, 100, or 1000 ng/L plus 1000 ng/L of TiO2 NPs) under static conditions for 144 hr. After exposure, BP-3 levels were determined by high-performance liquid chromatography (HPLC). BP-3 levels increased in the presence of TiO2 NPs, indicating that the BP-3 degradation decreased in the presence of the NPs. In addition, in the presence of zebrafish, BP-3 levels in water decreased, indicating that zebrafish embryos and larvae might absorb BP-3. Data demonstrated that, in general, environmentally relevant concentrations of BP-3 and TiO2 NPs, either alone or in a mixture, did not significantly induce changes in heart and spontaneous contractions frequencies, levels of reactive oxygen species (ROS), morphological and morphometric parameters as well as mortality rates during 144 hr exposure. However, the groups exposed to TiO2 NPs alone and in a mixture with BP-3 at 10 ng/L exhibited an earlier significant hatching rate than the controls. Altogether, the data indicates that a potential ecotoxicological impact on the aquatic environment exists.

Ecotoxicological assessment of effluents from Brazilian wastewater treatment plants using zebrafish embryotoxicity test: A multi-biomarker approach.

Effluent from wastewater treatment plants (WWTPs) remains one of the major pollutants of aquatic environments; however, knowledge about its ecotoxic effects at fish early life stages is limited. The ecotoxicity of effluent from Brazilian WWTPs was herein analyzed based on responses of multiple biomarkers in the zebrafish embryotoxicity test (ZET). Ecotoxicity was analyzed based on mortality rate, hatching rate, spontaneous movement rate (neurotoxicity), heart rate (cardiotoxicity), frequency of morphological changes and morphometric parameters during 144 h exposure time. Results showed that embryos exposed to affluent and effluent presented high mortality rate and delayed hatching rate, as well as changes in morphometric parameters. Exposed embryos also showed physiological, sensory, skeletal and muscular changes, which confirms that the ecotoxic effect of WWTPs effluent is systemic and associated with the presence of several pollutants, even at low concentrations (mixture toxicity). The present study is pioneer in using responses of multiple biomarkers in ZET as suitable approach to assess the ecotoxicity of WWTPs effluent in developing countries, as well as to add value and contribute to studies on WWTPs worldwide. Zebrafish is a suitable vertebrate model to assess the ecotoxicity of WWTP effluent.