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1.
Biochimie ; 2024 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-39461656

RESUMO

Hyperexpression of cathepsin B caused by an imbalance of endogenous inhibitors is involved in multiple pathologies, hence making it a key therapeutic target. Protease inhibitors are effective biomolecules that regulate protease activities and are considered potential therapeutic agents in various diseases. Plant protease inhibitors have been reported as an effective complementary alternative drug. A proteinaceous cathepsin B inhibitor (CBI-BP) has been isolated from Musa acuminata Colla (banana) peel with a molecular weight of 27.9 kDa on SDS-PAGE. The purity of the CBI-BP was confirmed on the native- PAGE. The isolated CBI-BP showed an IC50 value of 8.14 µg and a Ki value of 10.59 µg (0.19 µM). Cathepsin B inhibition kinetics indicated that CBI-BP follows a mixed-type of cathepsin B inhibition. Its inhibition activity was also confirmed by reverse zymography. The inhibitor was stable from pH 2.6 - 10.0 with maximum activity at pH 7.2, temperature 25-100°C and exhibited thermostability for 60 min at 70°C. MALDI/TOF/MS analysis of CBI-BP showed 40% similarity to the GH18 domain-containing protein (A0A4S8JRM9) from Musa balbisiana. Although in-silico docking studies showed binding of A0A4S8JRM9 to cathepsin B affects the binding energy of the substrate to cathepsin B but is not reported for any anti-cathepsin B activity. This suggests that isolated CBI-BP might be a novel protein with anti-cathepsin B activity. Thus the isolated CBI-BP may be further explored as possible anti-cathepsin B drug.

2.
Heliyon ; 10(19): e38781, 2024 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-39421385

RESUMO

Yogurt, a popular dairy product renowned for its nutritional benefits and probiotic content, serves as a functional food with potential health-promoting properties. The objective of this study was to investigate whether incorporating green banana peel polyphenol extract (GBPPE) into yogurt formulations enhances the viability and functionality of probiotics while also potentially improving the overall quality and health-promoting properties of the yogurts. GBPPE was extracted and added to the yogurt formulation at 0.0 %, 0.5 %, 1 %, and 2 %. Various physico-chemical properties of GBPPE as well as a range of physical, biochemical, sensory, and microbial assessments of formulated yogurts were carried out. Compared to the control, yogurt containing GBPPE improves functional characteristics by increasing antioxidant activity while having no detrimental impact on physicochemical and organoleptic properties. In terms of antioxidant capabilities, all fortified yogurts showed significantly (p < 0.05) higher total phenolic, flavonoid contents and antioxidant activities than the control yogurt. The addition of GBPPE also affected (p < 0.05) pH, titratable acidity, viscosity, water-binding capacity, syneresis, and total soluble solids, while no significant differences in the color parameters were detected in both control and all fortified yogurts with reduced brightness (L∗) and increased redness (a∗) of the product. The initial viable counts of all yogurt samples were almost similar, and the maximum and minimum viability loss of probiotics were observed in control and 2 % GBPPE fortified samples, respectively. Sensory assessment revealed that yogurt with 0.5 % banana peel extract outperformed all other treatments except the control. These findings support the sustainable use of GBPPE to create probiotic yogurt with improved physicochemical, microbiological, and sensory qualities.

3.
Int J Food Sci ; 2024: 2662967, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39132548

RESUMO

This research investigated the impact of enriching bread with banana peel powder (BPP) on nutritional attributes, bioactive components, antioxidant activity, and sensory characteristics. Four bread samples were prepared and evaluated: S1 (control), S2 (5% BPP), S3 (7% BPP), and S4 (10% BPP). The addition of BPP resulted in a reduction in moisture content and an increase in ash, fat, protein, and fibre levels, while reducing overall carbohydrate content. Furthermore, BPP-enriched bread exhibited an increase in total phenolic content (TPC) (ranging from 28.46 to 42.38 mg GAE/100 g) and total flavonoid content (TFC) (ranging from 6.63 to 9.46 QE mg/g), indicating enhanced antioxidant properties. The DPPH assay demonstrated the antioxidant potential of BPP-incorporated bread, with the radical scavenging activity (RSA) increasing from 18.84% to 53.03% with increasing BPP enrichment. Color assessment revealed changes in both crust and crumb, with a decrease from 78.46 to 40.53 in the lightness (L∗) value of the crust and from 61.21 to 41.10 in the lightness (L∗) value of the crumb. Additionally, changes in a∗ and b∗ values were observed. The a∗ values varied between 17.59 and 12.42 for the crust and between 6.96 and 5.89 for the crumb. The b∗ values varied between 31.61 and 23.65 for the crust and between 19.63 and 16.58 for the crumb. Sensory evaluation suggested that up to 5% BPP inclusion in bread mirrored the texture, taste, appearance, and overall acceptability of control bread, but enrichment beyond 5% resulted in lower sensory scores. In summary, the incorporation of BPP significantly influenced various aspects of bread, highlighting its potential for applications in the food and industry sectors.

4.
Foods ; 13(16)2024 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-39200570

RESUMO

Pure water scarcity is the most significant emerging challenge of the modern society. Various organics such as pesticides (clomazone, quinmerac), pharmaceuticals (ciprofloxacin, 17α-ethynilestradiol), and mycotoxins (deoxynivalenol) can be found in the aquatic environment. The aim of this study was to fabricate ZnO nanomaterial on the basis of banana peel extract (ZnO/BPE) and investigate its efficiency in the photocatalytic degradation of selected organics under various experimental conditions. Newly synthesized ZnO/BPE nanomaterials were fully characterized by the XRD, FTIR, SEM-EPS, XPS, and BET techniques, which confirmed the successful formation of ZnO nanomaterials. The photocatalytic experiments showed that the optimal catalyst loading of ZnO/BPE was 0.5 mg/cm3, while the initial pH did not influence the degradation efficiency. The reusability of the ZnO/BPE nanomaterial was also tested, and minimal activity loss was found after three photocatalytic cycles. The photocatalytic efficiency of pure banana peel extract (BPE) was also studied, and the obtained data showed high removal of ciprofloxacin and 17α-ethynilestradiol. Finally, the influence of water from Danube River was also examined based on the degradation efficiency of selected pollutants. These results showed an enhanced removal of ciprofloxacin in water from the Danube River, while in the case of other pollutants, the treatment was less effective.

5.
Food Chem X ; 23: 101539, 2024 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-38974193

RESUMO

The study addresses global fruit waste concerns in the food industry by extracting pectin from both ripe and unripe banana peels at varying pH levels and time intervals using hydrochloric acid. The best results were observed for unripe banana peel pectin at pH 1.5 and 250 min exhibiting a yield of 16.46% and favorable characteristics. In muffin development, seven treatments (M0, M1, M2, M3, M4, M5 and M6) are prepared and analyzed for morphology, nutritional content, and sensory parameters. The M4 treatment, utilizing pectin from unripe banana peel at pH 1.5 and 250 min, displays superior qualities with reduced peroxide value, free fatty acids, percent moisture loss, and hardness. Sensory evaluations indicate high acceptability due to lower fat content. In conclusion, the extraction of pectin from unripe banana peels proves promising as a fat replacer in bakery items, maintaining muffin quality while addressing fruit waste challenges in the food industry.

6.
Food Chem ; 460(Pt 1): 140549, 2024 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-39053277

RESUMO

This work investigated a sustainable and efficient approach of pectin extraction for banana peel waste valorisation and studied the influence of banana ripening stages (RS at 2,5 and 7). Although pectin content in banana peel raw material decreased during ripening, pectin extraction was favoured. The highest alcohol-insoluble solids (AIS) yield (12.5%) was achieved at 70 °C, 15 mins from RS 7 peel. All extracts were homogalacturan-rich with some rhamnogalacturonan-I content (showing HGA/RG-I ratio > 2) with varied degree of methylation (DM). The highest HGA content (837.2 mg/g AIS) and HGA/RG-I ratio (9.9) were achieved at 110 °C, 0 mins from RS 7, suggesting its promising application as gelling agent. The highest RG-I content (111.1 mg/g AIS) were obtained at 110 °C, 5 mins from RS 7, which was comparable with the pectin with reported prebiotic ability isolated from the literature, suggesting its potential application in novel products.


Assuntos
Estudos de Viabilidade , Frutas , Micro-Ondas , Musa , Pectinas , Extratos Vegetais , Pectinas/química , Pectinas/isolamento & purificação , Musa/química , Musa/crescimento & desenvolvimento , Frutas/química , Frutas/crescimento & desenvolvimento , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Fracionamento Químico/métodos
7.
Food Chem ; 448: 139176, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38574719

RESUMO

Using 3D printing technology, a gelatin-polyvinyl alcohol­carbon dots (GPC) layer+corn starch-polyvinyl alcohol-cinnamon essential oil (CPC) layer active bilayer film with an external barrier function and an internal controlled-release effect was successfully produced for food preservation. The GPC film was provided with potent antioxidant and UV blocking properties by the banana peel carbon dots (CDs). The cinnamon essential oil (CEO) had the strongest interaction with the film matrix at 3% (w/w), causing the CPC film having the lowest surface wettability, good integrity, and lowest crystallinity. The CEO's stability and releasing effectiveness were greatly enhanced by the creation of a bilayer film. At 60% filling rate of the CPC layer, the bilayer film showed the highest CEO retention after drying and the best CEO release performance. Finally, the created active bilayer film was found to significantly improve the sensory quality stability of the spicy essential oil microcapsule powders. It also successfully extended the mangoes' shelf life by delaying browning and rot.


Assuntos
Cinnamomum zeylanicum , Embalagem de Alimentos , Gelatina , Musa , Óleos Voláteis , Impressão Tridimensional , Amido , Óleos Voláteis/química , Embalagem de Alimentos/instrumentação , Cinnamomum zeylanicum/química , Gelatina/química , Amido/química , Musa/química , Carbono/química , Conservação de Alimentos/instrumentação , Conservação de Alimentos/métodos , Pontos Quânticos/química , Zea mays/química
8.
Materials (Basel) ; 17(7)2024 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-38611993

RESUMO

Porous anorthite (CaAl2Si2O8) ceramics, suitable for thermal insulation in buildings, were obtained using waste seashells as a source of CaO, kaolin as a source of Al2O3 and SiO2 and banana peel as a pore former. Changing the volume of banana peel as well as the processing temperature was found to be an effective approach to control the thermo-mechanical properties of the obtained anorthite ceramics. The sintering of powder compacts containing up to 30 wt% banana peel at temperatures ranging from 1100 to 1200 °C resulted in anorthite ceramics possessing up to 45% open porosity, a compressive strength between 13 and 92 MPa, a bulk density between 1.87 and 2.62 g/cm3 and thermal conductivity between 0.097 and 3.5 W/mK. It was shown that waste materials such as seashells and banana peel can be used to obtain cost-effective thermal insulation in buildings.

9.
Indian J Med Microbiol ; 48: 100558, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38458337

RESUMO

Lasiodiplodia theobromae is a dematiaceous fungus which rarely causes keratitis and is mostly resistant to the commonly used antifungal drugs. Here, we report three cases of keratitis caused by L.theobromae from Assam. All the cases were successfully treated with 1% voriconazole and surgical debridement. To the best of our knowledge and literature search, this is the first case series of keratitis caused by L.theobromae reported from eastern India.


Assuntos
Antifúngicos , Ascomicetos , Ceratite , Voriconazol , Humanos , Voriconazol/uso terapêutico , Antifúngicos/uso terapêutico , Ceratite/tratamento farmacológico , Ceratite/microbiologia , Índia , Masculino , Ascomicetos/isolamento & purificação , Ascomicetos/efeitos dos fármacos , Feminino , Adulto , Pessoa de Meia-Idade , Resultado do Tratamento , Micoses/tratamento farmacológico , Micoses/microbiologia , Desbridamento
10.
Environ Res ; 252(Pt 1): 118785, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38555094

RESUMO

The cube architecture associated with the CeO2 nanoflowers (NFs) that generated, which had an average crystallization width of 7 nm, has been confirmed by X-ray crystallographic investigations. The method used is environmentally acceptable since it converts wasted banana peel extracts into CeO2 nanoflower. On the basis of artwork obtained from a High-Resolution Transmission Electron Microscope (HR-TEM), CeO2 nanoparticles have been observed to possess a spherical shape and an average particle diameter of 21 nm. To take the purpose of this study, green-fabricated CeO2-NFs were used to investigate the photocatalytic oxidation of methyl orange (MO) dye when exposed to sunshine. CeO2 nanofibers showed a degradation performance of 98% when compared to methyl orange dye. Evidently is a possibility that this may be caused by the presence of CeO2 nanoflowers, whereby enhance the interaction of electrons, which are holes dissolution, and adherence. Upon a single day of being exposed, the biocidal potential was tested against both gram-positive and gram-negative bacteria, including E. coli, B. cereus, and S. aureus, among others. Due to the fact that its 32 mm minimum inhibitory concentration (MIC) for B. cereus was the highest among conventional medicines. As shown by the extraordinary capabilities of WBP@CeO2 tiny particles, manipulating of flexible tiny particles to feed the purpose of achieving effective and customizable infections and dermatologist advancements is really stunning.


Assuntos
Antibacterianos , Compostos Azo , Cério , Musa , Extratos Vegetais , Musa/química , Cério/química , Cério/farmacologia , Compostos Azo/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Antibacterianos/farmacologia , Antibacterianos/química , Corantes/química , Catálise , Testes de Sensibilidade Microbiana
11.
Molecules ; 29(5)2024 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-38474501

RESUMO

Banana peel waste is abundant and can be utilized as a low-cost adsorbent for removing toxic Cr (VI) from wastewater. The acid modification of banana peels significantly enhances their adsorption capacity for Cr (VI). An adsorbent was prepared by treating banana peel powder with 50% H2SO4 at 50 °C for 24 h. The acid treatment increased the surface area of the adsorbent from 0.0363 to 0.0507 m2/g. The optimum adsorbent dose was found to be 1 g/L for the complete removal of Cr (VI) from 100 ppm solutions. The adsorption capacity was 161 mg/g based on the Langmuir isotherm model. The adsorption kinetics followed a pseudo-second order model. Increasing the temperature from 20 to 50 °C increased the initial adsorption rate but had a minor effect on the equilibrium adsorption capacity. Thermodynamics studies showed that the process was spontaneous and endothermic. The activation energy was estimated as 24.5 kJ/mol, indicating physisorption. FTIR analyses before and after adsorption showed the involvement of hydroxyl, carbonyl and carboxyl groups in binding the Cr (VI). The Cr (VI) was reduced to Cr (III), which then bound to functional groups on the adsorbent. Desorption under acidic conditions could recover 36% of the adsorbed Cr as Cr (III). No desorption occurred at a neutral pH, indicating irreversible adsorption. Overall, acid-modified banana peel is an efficient, low-cost and eco-friendly adsorbent for removing toxic Cr (VI) from wastewater.

12.
Materials (Basel) ; 17(2)2024 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-38255479

RESUMO

Adopting banana peel as a raw material, the adsorption properties of banana peel hydrothermal carbon modified with a KOH solution for lead ions in aqueous solution were studied. The surface structure and functional groups of the modified hydrothermal carbon were analyzed by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared (FT-IR) spectroscopy, the Brunner-Emmet-Teller (BET) method, element analysis, and Raman spectroscopy. The results showed that an adsorption capacity of 42.92 mg/g and a removal rate of 86.84% were achieved when the banana peel hydrothermal carbon was modified with a KOH solution of 0.5 mol/L, with a pH of 6 and a solid-liquid ratio of 1 g/L. The equilibrium adsorption time for lead ions in solution being adsorbed using KOH-modified hydrothermal carbon was 240 min, the adsorption process satisfied the quasi-second-order kinetic model and the Redlich-Peterson isotherm equation, and the equilibrium removal efficiency was 88.62%. The adsorption of lead ions using KOH-modified hydrothermal carbon is mainly chemical-physical adsorption.

13.
Environ Sci Pollut Res Int ; 31(9): 13753-13765, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38265588

RESUMO

In the realm of environmental challenges, microplastics have emerged as a pressing threat, presenting risks to both individuals and ecosystems. Conventional treatment plants are presently not equipped for effectively removing these minute contaminants. This study presents an investigation into the potential of a continuous flow biochar column, utilizing biochar derived from banana peel through a nitrogen-free slow pyrolysis process for the removal of microplastics. A systematic exploration of various parameters, including bed height, flow rate, inflow microplastic concentration, and microplastic size is undertaken to discern their impact on polystyrene removal efficiency. A peak removal efficiency of 92.16% has been achieved under specific conditions: a 6-cm bed height, a 3-mL/min flow rate, an inlet concentration of 0.05 g/L, and microplastic sizes ranging from 150 to 300 µm. The removal efficiency was inversely affected by flow rate while directly influenced by bed height. To deepen the understanding of polystyrene removal on biochar, a detailed characterization of the synthesized material was carried out. The removal of microplastics by banana peel biochar (BPB) is observed to be dominated by adsorption and filtration processes. The entanglement of microplastics with minuscule biochar granules, capture between particles, and entrapment in the porous system were identified as the mechanisms of removal. Leveraging the hydrophobic nature of polystyrene microplastics, interactions with the hydrophobic functional groups in BPB result in effective adsorption. This is further complemented by self-agglomeration and filtration mechanisms that synergistically contribute to the elimination of larger agglomerates. The findings thus provide a comprehensive understanding, offering hope for a more effective strategy in mitigating the environmental impact of microplastics.


Assuntos
Microplásticos , Poluentes Químicos da Água , Humanos , Poliestirenos , Plásticos , Ecossistema , Poluentes Químicos da Água/análise , Carvão Vegetal/química , Adsorção
14.
Int J Biol Macromol ; 256(Pt 2): 128460, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38035954

RESUMO

5-F-lourouracil is an anticancer drug used for the treatment of different types of cancers. 5-flourouracil loaded chitosan-banana peel extract films were prepared for the in vitro drug release studies. Solvent casting technique was employed to prepare the films. The structure and morphology of the prepared films were analysed by FTIR, XRD and SEM methods and confirmed the presence of drug in the films. The drug loaded films show excellent thermal stability and good shelf life. Studies revealed that the percentage of banana peel extract influences the swelling properties of the film, thickness of the films and release of the drug from the films. Increase in the concentration of chitosan and banana peel extract decreases the swelling properties of the film and rate of release of the drug from the film. The release rate of 5-fluorouracil from the drug loaded chitosan-banana peel extract films were followed spectrophotometrically at λmax 266 nm and the film derived from 1 % chitosan solution and 1 mL aqueous banana peel extract (saturated) exhibited maximum drug release. Cytotoxicity studies proved that the films are non-toxic in nature and augurs well for their applications as excellent drug delivery systems. Antimicrobial studies show that the drug loaded chitosan-banana peel extract films were found to be active against microbes E. coli, Streptococcus mutans, Staphylococcus aureus, Candida albicans and Aspergillus niger and inactive against Pseudomonas aeruginosa.


Assuntos
Antineoplásicos , Quitosana , Musa , Quitosana/química , Fluoruracila/farmacologia , Escherichia coli , Antineoplásicos/farmacologia
15.
Appl Biochem Biotechnol ; 196(1): 491-505, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37145344

RESUMO

The current study reports the synthesis of sustainable nano-hydroxyapatite (nHAp) using a wet chemical precipitation approach. The materials used in the green synthesis of nHAp were obtained from environmental biowastes such as HAp from eggshells and pectin from banana peels. The physicochemical characterization of obtained nHAp was carried out using different techniques. For instance, X-ray diffractometer (XRD) and FTIR spectroscopy were used to study the crystallinity and synthesis of nHAp respectively. In addition, the morphology and elemental composition of nHAP were studied using FESEM equipped with EDX. HRTEM showed the internal structure of nHAP and calculated its grain size which was 64 nm. Furthermore, the prepared nHAp was explored for its antibacterial and antibiofilm activity which has received less attention previously. The obtained results showed the potential of pectin-bound nHAp as an antibacterial agent for various biomedical and healthcare applications.


Assuntos
Durapatita , Pectinas , Animais , Durapatita/química , Pectinas/farmacologia , Casca de Ovo , Antibacterianos/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier
16.
MethodsX ; 12: 102522, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38111791

RESUMO

Herein, we demonstrate the simple one-pot novel green synthesis of gold nanoparticles (Au-NPs) functionalised with a combination of banana peel (Musa paradaisica) and grape (Vitis vinifera) fruit extracts. The reaction mixture of aqueous gold chloride, banana peel and grape extracts revealed a purple colour after a reaction time of one hour, an indication of the presence and the successful synthesis of gold nanoparticles. The optical and structural properties of the green synthesized nanoparticles were analysed using Ultraviolet-Visible spectroscopy (UV-Vis) and Fourier Transform Infrared Spectroscopy (FTIR) while their surface morphology was determined using X-Ray Diffraction (XRD), High-Resolution Transmission Microscopy (HRTEM) and Small Angle X-Ray (SAX). Furthermore, a quick and simple surface plasmon resonance (SPR) study in the form of an optical sensor for the detection of Escherichia coli 0157:H7 strain was also achieved using UV-Vis. The obtained limit of detection (LOD) value for SPR for the GBPE|Au-NPs|GCE-based system was found to be 1 × 102 CFU/mL, a value well in the range for detection in seawater.•Green synthesis of gold nanoparticles (Au-NPs) was functionalised using banana peel (Musa paradaisica) and grape (Vitis vinifera) fruit extracts as capping and stabilizing agents.•Structural characterization of the Au-NPs was achieved using Ultraviolet-Visible spectroscopy (UV-Vis) and Fourier Transform Infrared Spectroscopy (FTIR) while their surface morphology was determined using X-Ray Diffraction (XRD), High-Resolution Transmission Microscopy (HRTEM) and Small Angle X-Ray (SAX).•The green synthesized Au-NPs were used to detect Escherichia coli 0157:H7 (E. coli 0157:H7) strain using Ultraviolet-Visible spectroscopy (UV-Vis) where the surface plasmon resonance (SPR) was studied.

17.
Indian J Microbiol ; 63(4): 398-409, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-38031613

RESUMO

The production of banana peel by the food-processing industry is substantial and the disposal of this waste material has become a matter of concern. However, recent studies have demonstrated that banana peel is a rich source of biologically active compounds that can be transformed into valuable products. This review aims to explore the potential of converting banana peel into valuable products and provides a comprehensive analysis of the physical and chemical composition of banana peel. Additionally, the utilization of banana peel as a substrate to produce animal feed, bio fertilizer, dietary fibers, renewable energy, industrial enzymes, and nanomaterials has been extensively studied. According to the researches that has been done so far, it is clear that banana peel has a broad range of applications and its effective utilization through biorefinery strategies can maximize its economic benefits. Based on previous studies, A plan for feasibility of a banana peel biorefinery has been put up which suggest its potential as a valuable source of renewable energy and high-value products. The utilization of banana peel through biorefinery strategies can provide a sustainable solution for waste management and contribute to the development of a circular economy.

18.
Biotechnol Rep (Amst) ; 40: e00815, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37876548

RESUMO

The growing demand for renewable energy sources such as bioethanol is facing a lack of efficient ethanologenic microbes. This study aimed to isolate and screen ethanologenic yeasts from Ethiopian fermented beverages. A progressive screening and selection approach was employed. Selected isolates were evaluated for bioethanol production using banana peel waste as substrate. A total of 102 isolates were obtained. Sixteen isolates were selected based on their tolerance to stress conditions and carbohydrate fermentation and assimilation capacity. Most found moderately tolerant to 10 %, but slightly tolerant at 15 and 20 % (v/v) ethanol concentration. They yield 15.3 to 20.1 g/L and 9.1 ± 0.6 to 12.9 ± 1.3 g/L ethanol from 2 % (w/v) glucose and 80 g/L banana peel, respectively. Molecular characterization identified them as Saccharomyces cerevisiae strains. Results demonstrate insight about their potential role in the ethanol industry. Optimization of the fermentation conditions is recommended.

19.
Braz J Microbiol ; 54(4): 2745-2753, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37872277

RESUMO

Bacterial cellulose (BC) is an exopolysaccharide produced by bacteria that has unusual structural features and is more refined than plant cellulose. BC has recently gained more attention in a variety of fields including biological and biomedical applications due to its excellent physiochemical properties including easy biodegradability, better water holding capacity, high tensile strength, high thermal stability, and high degree of polymerization. However, application of BC at industrial scale is still limited due to its high production cost and lesser yielding strains. The present study is an attempt to isolate and characterize a novel BC-producing bacterial strain. The bacterial strain S5 has resulted into maximum cellulose production of 4.76 ± 0.49 gL-1 (30°C, pH 7.0). The strain has been further identified as Stenotrophomonas sp. Derivation of nutritional and cultural conditions has resulted into 2.34-fold enhanced BC production (banana peel powder, peptone, tartaric acid, pH 7, 30°C). FTIR spectrum of BC revealed characteristic absorption bands which could be attributed to the O-H band, C-H stretching, C-O-C stretching band, O-H bending, and >CH2 bending, indicative of the ß-1,4 glycosidic linkages of cellulose. Thermogravimetric analysis has also revealed stability of polysaccharide backbones and characteristic weight loss points. Employment of banana peel powder has appeared as a proficient low-cost source for large-scale economic production of BC for industrial applications.


Assuntos
Musa , Celulose , Análise Custo-Benefício , Pós , Bactérias/genética
20.
Water Environ Res ; 95(10): e10938, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37815304

RESUMO

In this study, the electrocoagulation (EC) treatment was used to minimize and separate pollutants from textile industrial wastewater (TIWW), including high color, chemical oxygen demand (COD), total organic carbon (TOC), and total dissolved solids (TDS). To enhance the EC treatment efficiency, a novel strategy has been followed in the study that involves thin-film coating on 316 stainless steel (SS) electrodes with banana peel-derived activated carbon (BPAC) by dip coating, spin coating, or spray coating. Among the different types of coating, thickness and contact angle measurements have elucidated that the spray coating of BPAC on SS electrode is the best tool with minimum thickness and contact angle. In this study, a bare SS electrode was used as the anode and a thin-film spray-coated BPAC on the SS electrode was used as the cathode. Moreover, optimization plays a key role in EC treatment process, where operating conditions such as a current density of 10 mA/cm2 , contact time of 15 min, and a pH of 7 were fixed. As a result, the findings indicate comparatively high color removal of 98%, COD removal of 91%, TOC removal of 89.6%, and TDS removal of 68% are achieved with ease. Accordingly, in comparison with plain SS electrodes or dip- or spin-coated BPAC on SS electrodes, spray-coated BPAC on SS electrodes in EC treatment outperforms in removing high color, TOC, COD, and TDS. Overall, the study highlights the potential of EC treatment integrated with adsorption procedures for TIWW treatment. Particularly, the use of thin-film spray-coated BPAC on SS electrodes in the EC treatment process led to an effective and sustainable tool for treating and reuse of TIWW. It is due to its low operation and maintenance cost and studied in a short interval of time. Finally, the ultimate goal was firmly achieved in pilot-scale studies by the safe discharge into the environment or reuse of treated textile wastewater. Thus, it is a promising alternative with an environmentally friendly footprint that could be easily implemented in any textile industry premises. PRACTITIONER POINTS: Heavy metals, oils, facts, suspended solids, and other pollutants can be removed from industrial effluent by using electrocoagulation. The process is both cost-effective and energy-efficient, and it is easily integrated with other water treatment technologies. According to the findings of this study, minimum current density should be applied to BPAC-SS-coated electrodes by DC power supplies to treat textile industry effluents at low operating costs. When compared with a plain SS electrode, the spray-coated BPAC on SS electrode provides better performance in effluent treatment.


Assuntos
Poluentes Ambientais , Águas Residuárias , Aço Inoxidável , Carvão Vegetal , Eletrocoagulação/métodos , Têxteis , Eletrodos , Eliminação de Resíduos Líquidos/métodos , Resíduos Industriais
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