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1.
Ecol Evol ; 14(7): e11659, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38957698

RESUMO

Quantifying the cost-effectiveness of alternative sampling methods is crucial for efficient biodiversity monitoring and detection of population trends. In this study, we compared the cost-effectiveness of three novel sampling methods for detecting changes in koala (Phascolarctos cinereus) occupancy: thermal drones, passive acoustic recorders and camera trapping. Specifically, we fitted single-season occupancy-detection models to data recorded from 46 sites in eight bioregions of New South Wales, Australia, between 2018 and 2022. We explored the effect of weather variables on daily detection probability for each method and, using these estimates, calculated the statistical power to detect 30%, 50% and 80% declines in koala occupancy. We calculated power for different combinations of sites (1-200) and repeat surveys (2-40) and developed a cost model that found the cheapest survey design that achieved 80% power to detect change. On average, detectability of koalas was highest with one 24-h period of acoustic surveys (0.32, 95% CI's: 0.26, 0.39) compared to a 25-ha flight of drone surveys (0.28, 95% 0.15, 0.48) or a 24-h period of camera trapping consisting of six cameras (0.019, 95% CI's: 0.014, 0.025). We found a negative quadratic relationship between detection probability and air temperature for all three methods. Our power and cost analysis suggested that 148 sites surveyed with acoustic recorders deployed for 14 days would be the cheapest method to sufficiently detect a 30% decline in occupancy with 80% power. We recommend passive acoustic recorders as the most efficient sampling method for monitoring koala occupancy compared to cameras or drones. Further comparative studies are needed to compare the relative effectiveness of these methods and others when the monitoring objective is to detect change in koala abundance over time.

2.
J Fish Biol ; 2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-38965826

RESUMO

Basking sharks (Cetorhinus maximus) seasonally aggregate in coastal surface waters of the North Atlantic, providing opportunities for visual observation. While putative courtship displays have been observed, actual copulation has not been documented. Here we examine video collected by an unmanned aerial vehicle ("drone") of novel behavioral interactions between basking sharks in Cape Cod Bay, Massachusetts in May 2021. The behaviors, including close following and tight concentric circling, are consistent with pre-copulatory behavior observed in other shark species. These observations provide new insights into the pre-copulatory behavior of basking sharks.

3.
Environ Monit Assess ; 196(8): 694, 2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-38963575

RESUMO

Human activities at sea can produce pressures and cumulative effects on ecosystem components that need to be monitored and assessed in a cost-effective manner. Five Horizon European projects have joined forces to collaboratively increase our knowledge and skills to monitor and assess the ocean in an innovative way, assisting managers and policy-makers in taking decisions to maintain sustainable activities at sea. Here, we present and discuss the status of some methods revised during a summer school, aiming at better management of coasts and seas. We include novel methods to monitor the coastal and ocean waters (e.g. environmental DNA, drones, imaging and artificial intelligence, climate modelling and spatial planning) and innovative tools to assess the status (e.g. cumulative impacts assessment, multiple pressures, Nested Environmental status Assessment Tool (NEAT), ecosystem services assessment or a new unifying approach). As a concluding remark, some of the most important challenges ahead are assessing the pros and cons of novel methods, comparing them with benchmark technologies and integrating these into long-standing time series for data continuity. This requires transition periods and careful planning, which can be covered through an intense collaboration of current and future European projects on marine biodiversity and ecosystem health.


Assuntos
Biodiversidade , Conservação dos Recursos Naturais , Ecossistema , Monitoramento Ambiental , Monitoramento Ambiental/métodos , Conservação dos Recursos Naturais/métodos , Humanos , Oceanos e Mares , Atividades Humanas
4.
BMC Ecol Evol ; 24(1): 89, 2024 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-38956462

RESUMO

Galician forests in northwestern Spain are subject to frequent wildfires with high environmental and economic costs. In addition, due to the consequences of climate change, these fires are becoming more virulent, occurring throughout the year, and taking place in populated areas, in some cases involving the loss of human life. Therefore, forest fire prevention is even more relevant than mitigating its consequences. Given the costs involved in forestry work, alternative measures to reduce fuel load and create vegetation gaps are needed. One involves grazing by an endemic species of feral horses (Equus ferus atlanticus) that feed on thicket-forming gorse (Ulex europaeus). In a 100-ha forest fenced study area stocked with 11 horses, four 50 m2 enclosed plots prevented the access of these wild animals to the vegetation, with the aim of manipulating their impact on the reduction of forest biomass. The measurement of biomass volumes is an important method that can describe the assessment of wildfire risks, unfortunately, high-resolution data collection at the regional scale is very time-consuming. The best result can be using drones (unmanned aerial vehicles - UAVs) as a method of collecting remotely sensed data at low cost. From September 2018 to November 2020, we collected information about aboveground biomass from these four enclosed plots and their surrounding areas available for horses to forage, via UAV. These data, together with environmental variables from the study site, were used as input for a fire model to assess the differences in the surface rate of spread (SROS) among grazed and ungrazed areas. Our results indicated a consistent but small reduction in the SROS between 0.55 and 3.10 m/min in the ungrazed enclosured plots in comparison to their grazed surrounding areas (which have an SROS between 15 and 25 m/min). The research showed that radar remote sensing (UAV) can be used to map forest aboveground biomass, and emphasized the importance and role of feral horses in Galicia as a prevention tool against wildfires in gorse-dominated landscapes.


Assuntos
Biomassa , Tecnologia de Sensoriamento Remoto , Animais , Cavalos/fisiologia , Espanha , Tecnologia de Sensoriamento Remoto/métodos , Florestas , Pradaria , Incêndios Florestais , Conservação dos Recursos Naturais/métodos
5.
Sensors (Basel) ; 24(11)2024 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-38894139

RESUMO

This paper presents an overview on the state of the art in copter drones and their components. It starts by providing an introduction to unmanned aerial vehicles in general, describing their main types, and then shifts its focus mostly to multirotor drones as the most attractive for individual and research use. This paper analyzes various multirotor drone types, their construction, typical areas of implementation, and technology used underneath their construction. Finally, it looks at current challenges and future directions in drone system development, emerging technologies, and future research topics in the area. This paper concludes by highlighting some key challenges that need to be addressed before widespread adoption of drone technologies in everyday life can occur. By summarizing an up-to-date survey on the state of the art in copter drone technology, this paper will provide valuable insights into where this field is heading in terms of progress and innovation.

6.
Glob Chang Biol ; 30(6): e17366, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38847450

RESUMO

Changes in body size have been documented across taxa in response to human activities and climate change. Body size influences many aspects of an individual's physiology, behavior, and ecology, ultimately affecting life history performance and resilience to stressors. In this study, we developed an analytical approach to model individual growth patterns using aerial imagery collected via drones, which can be used to investigate shifts in body size in a population and the associated drivers. We applied the method to a large morphological dataset of gray whales (Eschrichtius robustus) using a distinct foraging ground along the NE Pacific coast, and found that the asymptotic length of these whales has declined since around the year 2000 at an average rate of 0.05-0.12 m/y. The decline has been stronger in females, which are estimated to be now comparable in size to males, minimizing sexual dimorphism. We show that the decline in asymptotic length is correlated with two oceanographic metrics acting as proxies of habitat quality at different scales: the mean Pacific Decadal Oscillation index, and the mean ratio between upwelling intensity in a season and the number of relaxation events. These results suggest that the decline in gray whale body size may represent a plastic response to changing environmental conditions. Decreasing body size could have cascading effects on the population's demography, ability to adjust to environmental changes, and ecological influence on the structure of their community. This finding adds to the mounting evidence that body size is shrinking in several marine populations in association with climate change and other anthropogenic stressors. Our modeling approach is broadly applicable across multiple systems where morphological data on megafauna are collected using drones.


Assuntos
Tamanho Corporal , Mudança Climática , Baleias , Animais , Feminino , Masculino , Baleias/fisiologia , Ecossistema , Modelos Biológicos , Oceano Pacífico
7.
Sci Rep ; 14(1): 12506, 2024 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-38822002

RESUMO

The infiltration of heterogenous fleets of autonomous Unmanned Aerial Vehicles (UAVs) in smart cities is leading to the consumerization of city air space which includes infrastructure creation of roads, traffic design, capacity estimation, and trajectory optimization. This study proposes a novel autonomous Advanced Aerial Mobility (AAM) logistical system for high density city centers. First, we propose a real-time 3D geospatial mining framework for LiDAR data to create a dynamically updated digital twin model. This enables the identification of viable airspace volumes in densely populated 3D environments based on the airspace policy/regulations. Second, we propose a robust city airspace dynamic 4D discretization method (Skyroutes) for autonomous UAVs to incorporate the underlying real-time constraints coupled with externalities, legal, and optimal UAV operation based on kinematics. An hourly trip generation model was applied to create 1138 trips in two scenarios comparing the cartesian discretization to our proposed algorithm. The results show that the AAM enables a precise airspace capacity/cost estimation, due to its detailed 3D generation capabilities. The AAM increased the airspace capacity by up to 10%, the generated UAV trajectories are 50% more energy efficient, and significantly safer.

8.
Sci Total Environ ; 934: 173213, 2024 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-38750739

RESUMO

Uncrewed Aerial Spray Systems (UASS), commonly called drones, have become an important application technique for plant protection products in Asia and worldwide. As such, environmental variables and spray system parameters influencing spray drift deserve detailed investigations. This study presents the data analysis of 114 UASS drift trials conducted between December 2021 and December 2022 in China. Study design was based on the ISO 22866:2005 protocol for spray drift trials and considered different UASS platforms, nozzles, and release heights, and specifically continuously measured weather conditions. The relative importance of the environmental variables and spray system parameters was evaluated by a random forest (RF) feature importance analysis, a Sobol sensitivity analysis and partial dependence plots. This approach was preferred to linear ranking techniques such as ANOVA (analysis of variance) due to the non-linearity of the system. In addition, partial dependence plots are proposed to visualize the relationship between specific input parameters within the system. Drift deposition curves calculated from the 114 trials show good agreement with previous UASS trials reported in the literature. As reported in previous studies, spray drift following UASS applications is lower than for manned aerial vehicles, greater than for ground spray applications, and similar to drift observed from orchard air blast applications. In addition, 9 trials were conducted on corn fields in order to evaluate the potential effect of crop cover on spray drift. Spray drift was observed to be reduced over the cropped soil, suggesting that plant cover might possibly reduce spray drift. These findings could help supporting drift mitigation policies, stewardship advice and product labelling around the world.

9.
Sensors (Basel) ; 24(10)2024 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-38793891

RESUMO

In response to the numerous challenges faced by traditional human pose recognition methods in practical applications, such as dense targets, severe edge occlusion, limited application scenarios, complex backgrounds, and poor recognition accuracy when targets are occluded, this paper proposes a YOLO-Pose algorithm for human pose estimation. The specific improvements are divided into four parts. Firstly, in the Backbone section of the YOLO-Pose model, lightweight GhostNet modules are introduced to reduce the model's parameter count and computational requirements, making it suitable for deployment on unmanned aerial vehicles (UAVs). Secondly, the ACmix attention mechanism is integrated into the Neck section to improve detection speed during object judgment and localization. Furthermore, in the Head section, key points are optimized using coordinate attention mechanisms, significantly enhancing key point localization accuracy. Lastly, the paper improves the loss function and confidence function to enhance the model's robustness. Experimental results demonstrate that the improved model achieves a 95.58% improvement in mAP50 and a 69.54% improvement in mAP50-95 compared to the original model, with a reduction of 14.6 M parameters. The model achieves a detection speed of 19.9 ms per image, optimized by 30% and 39.5% compared to the original model. Comparisons with other algorithms such as Faster R-CNN, SSD, YOLOv4, and YOLOv7 demonstrate varying degrees of performance improvement.


Assuntos
Algoritmos , Postura , Humanos , Postura/fisiologia , Dispositivos Aéreos não Tripulados , Processamento de Imagem Assistida por Computador/métodos
10.
Front Immunol ; 15: 1366962, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38736880

RESUMO

Hematopoietic stem cell transplantation and cell therapies like CAR-T are costly, complex therapeutic procedures. Outpatient models, including at-home transplantation, have been developed, resulting in similar survival results, reduced costs, and increased patient satisfaction. The complexity and safety of the process can be addressed with various emerging technologies (artificial intelligence, wearable sensors, point-of-care analytical devices, drones, virtual assistants) that allow continuous patient monitoring and improved decision-making processes. Patients, caregivers, and staff can also benefit from improved training with simulation or virtual reality. However, many technical, operational, and above all, ethical concerns need to be addressed. Finally, outpatient or at-home hematopoietic transplantation or CAR-T therapy creates a different, integrated operative system that must be planned, designed, and carefully adapted to the patient's characteristics and distance from the hospital. Patients, clinicians, and their clinical environments can benefit from technically improved at-home transplantation.


Assuntos
Transplante de Células-Tronco Hematopoéticas , Serviços de Assistência Domiciliar , Humanos , Transplante de Células-Tronco Hematopoéticas/métodos , Imunoterapia Adotiva/métodos , Inteligência Artificial
11.
Sensors (Basel) ; 24(8)2024 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-38676050

RESUMO

The use of drones has recently gained popularity in a diverse range of applications, such as aerial photography, agriculture, search and rescue operations, the entertainment industry, and more. However, misuse of drone technology can potentially lead to military threats, terrorist acts, as well as privacy and safety breaches. This emphasizes the need for effective and fast remote detection of potentially threatening drones. In this study, we propose a novel approach for automatic drone detection utilizing the usage of both radio frequency communication signals and acoustic signals derived from UAV rotor sounds. In particular, we propose the use of classical and deep machine-learning techniques and the fusion of RF and acoustic features for efficient and accurate drone classification. Distinct types of ML-based classifiers have been examined, including CNN- and RNN-based networks and the classical SVM method. The proposed approach has been evaluated with both frequency and audio features using common drone datasets, demonstrating better accuracy than existing state-of-the-art methods, especially in low SNR scenarios. The results presented in this paper show a classification accuracy of approximately 91% at an SNR ratio of -10 dB using the LSTM network and fused features.

12.
Resusc Plus ; 18: 100633, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38666251

RESUMO

Intro: Medical drones are an emerging technology which may facilitate rapid treatment in time-sensitive emergencies. However, drones rely on lay rescuers, whose interactions with multipurpose medical drones have not been studied, and the optimal drone design remains unclear. Methods: We conducted 24 simulations of adult out-of-hospital cardiac arrest (OHCA) and pediatric anaphylaxis with a prototype drone equipped with spoken and visual cues and a multipurpose medical kit. 24 layperson volunteers encountered one of the two scenarios and were supported through administering treatment by a simulated 911 dispatcher. Bystander-drone interactions were evaluated via a convergent parallel mixed methods approach using surveys, video event review, and semi-structured interviews. Results: 83% (20/24) of participants voiced comfort interacting with the drone. 96% (23/24) were interested in future interaction. Participants appreciated the drone's spoken instructions but found visual cues confusing. Participants retrieved the medical kit from the drone in a mean of 5 seconds (range 2-14) of drone contact; 79% (19/24) found this step easy or very easy. The medical kit's layered design caused difficulty in retrieving appropriate equipment. Participants expressed a wide range of reactions to the unique drone design. Conclusions: Laypeople can effectively and comfortably interact with a medical drone with a novel design. Feedback on design elements will result in further refinements and valuable insights for other drone designers. A multipurpose medical kit created more challenges and indicates the need for further refinement to facilitate use of the equipment.

13.
Mol Ecol Resour ; : e13957, 2024 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-38576153

RESUMO

In coastal British Columbia, Canada, marine megafauna such as humpback whales (Megaptera novaeangliae) and fin whales (Balaenoptera physalus velifera) have been subject to a history of exploitation and near extirpation. While their populations have been in recovery, significant threats are posed to these vulnerable species by proposed natural resource ventures in this region, in addition to the compounding effects of anthropogenic climate change. Genetic tools play a vital role in informing conservation efforts, but the associated collection of tissue biopsy samples can be challenging for the investigators and disruptive to the ongoing behaviour of the targeted whales. Here, we evaluate a minimally intrusive approach based on collecting exhaled breath condensate, or respiratory 'blow' samples, from baleen whales using an unoccupied aerial system (UAS), within Gitga'at First Nation territory for conservation genetics. Minimal behavioural responses to the sampling technique were observed, with no response detected 87% of the time (of 112 UAS deployments). DNA from whale blow (n = 88 samples) was extracted, and DNA profiles consisting of 10 nuclear microsatellite loci, sex identification and mitochondrial (mt) DNA haplotypes were constructed. An average of 7.5 microsatellite loci per individual were successfully genotyped. The success rates for mtDNA and sex assignment were 80% and 89% respectively. Thus, this minimally intrusive sampling method can be used to describe genetic diversity and generate genetic profiles for individual identification. The results of this research demonstrate the potential of UAS-collected whale blow for conservation genetics from a remote location.

14.
Pest Manag Sci ; 80(8): 4074-4084, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38563560

RESUMO

BACKGROUND: Halyomorpha halys is one of the most damaging invasive agricultural pests in North America and southern Europe. It is commonly monitored using pheromone traps, which are not very effective because few bugs are caught and some escape and/or remain outside the trap on surrounding plants where they feed, increasing the damage. Other monitoring techniques are based on visual sampling, sweep-netting and tree-beating. However, all these methods require several hours of human labor and are difficult to apply to large areas. The aim of this work is to develop an automated monitoring system that integrates image acquisition through the use of drones with H. halys detection through the use of artificial intelligence (AI). RESULTS: The study results allowed the development of an automated flight protocol using a mobile app to capture high-resolution images. The drone caused only low levels of disturbance in both adult and intermediate instars, inducing freezing behavior in adults. Each of the AI models used achieved very good performance, with a detection accuracy of up to 97% and recall of up to 87% for the X-TL model. CONCLUSION: The first application of this novel monitoring system demonstrated the potential of drones and AI to detect and quantify the presence of H. halys. The ability to capture high-altitude, high-resolution images makes this method potentially suitable for use with a range of crops and pests. © 2024 Society of Chemical Industry.


Assuntos
Inteligência Artificial , Controle de Insetos , Dispositivos Aéreos não Tripulados , Animais , Controle de Insetos/métodos , Controle de Insetos/instrumentação , Heterópteros/fisiologia , Ninfa/fisiologia , Ninfa/crescimento & desenvolvimento
15.
Artigo em Inglês | MEDLINE | ID: mdl-38472584

RESUMO

Honey bees are social insects that show division of labor and sexual dimorphism. Female honey bees differentiate in two different castes, queens or worker bees, while males are called drones. Worker bees have different tasks in the hive including collection of food, its processing, caring for brood, protecting the hive, or producing wax. The drones' only role is to mate with a virgin queen. Many studies have dealt with differences in physiology, behavior, and morphology of workers and drones. This is the first study that demonstrates differences in element accumulation and composition between workers and drones honey bees. Using inductively coupled plasma mass spectrometry, we found that worker honey bees have higher concentrations of most elements analyzed. Drones had higher concentrations of elements essential to bees, Na, P, S, Zn, Cu, and especially Se (2.2 × higher), which is known to be important for sperm quality and fertility in many animals. Until now higher Se content was not observed in male insects. These differences can be attributed to different environmental exposure, reproductive role of drones, but mostly to the food workers and drones consume. Worker bees feed on bee bread, which is rich in minerals. Drones are fed food pre-processed by worker bees.

16.
Heliyon ; 10(5): e26913, 2024 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-38455562

RESUMO

Smallholder farms are major contributors to agricultural production, food security, and socio-economic growth in many developing countries. However, they generally lack the resources to fully maximize their potential. Subsequently they require innovative, evidence-based and lower-cost solutions to optimize their productivity. Recently, precision agricultural practices facilitated by unmanned aerial vehicles (UAVs) have gained traction in the agricultural sector and have great potential for smallholder farm applications. Furthermore, advances in geospatial cloud computing have opened new and exciting possibilities in the remote sensing arena. In light of these recent developments, the focus of this study was to explore and demonstrate the utility of using the advanced image processing capabilities of the Google Earth Engine (GEE) geospatial cloud computing platform to process and analyse a very high spatial resolution multispectral UAV image for mapping land use land cover (LULC) within smallholder farms. The results showed that LULC could be mapped at a 0.50 m spatial resolution with an overall accuracy of 91%. Overall, we found GEE to be an extremely useful platform for conducting advanced image analysis on UAV imagery and rapid communication of results. Notwithstanding the limitations of the study, the findings presented herein are quite promising and clearly demonstrate how modern agricultural practices can be implemented to facilitate improved agricultural management in smallholder farmers.

17.
PeerJ Comput Sci ; 10: e1759, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38435606

RESUMO

This article introduces a prototype laser communication system integrated with uncrewed aerial vehicles (UAVs), aimed at enhancing data connectivity in remote healthcare applications. Traditional radio frequency systems are limited by their range and reliability, particularly in challenging environments. By leveraging UAVs as relay points, the proposed system seeks to address these limitations, offering a novel solution for real-time, high-speed data transmission. The system has been empirically tested, showcasing its ability to maintain data transmission integrity under various conditions. Results indicate a substantial improvement in connectivity, with high data transmission success rate (DTSR) scores, even amidst environmental disturbances. This study underscores the system's potential for critical applications such as emergency response, public health monitoring, and extending services to remote or underserved areas.

18.
Artigo em Inglês | MEDLINE | ID: mdl-38441422

RESUMO

Sudden cardiac arrest remains a relevant problem with a significant number of deaths worldwide. Although survival rates have more than tripled over the last 20 years (4% in 2001 vs. 14% in 2020), survival rates with good neurological outcomes remain persistently low, representing a major socioeconomic problem. Every minute of delay from patient collapse to start cardiopulmonary resuscitation (CPR) and early defibrillation reduces the chance of survival by approximately 10-12%. Therefore, the time to treatment is a crucial factor in the prognosis of patients with out-of-hospital cardiac arrest (OHCA). Research teams working in the pre-hospital setting are therefore looking for ways to improve the transmission of information from the site of an emergency event and to make it easier for emergency medical dispatch centres (EMDC) to recognise life-threatening conditions with minimal deviation. For emergency unit procedures already at the scene of the event, methods are being sought to efficiently and temporarily replace a non-functioning cardiopulmonary system. In the case of traumatic cardiac arrest (TCA), the focus is mainly on effective affecting non-compressible haemorrhage.


Assuntos
Reanimação Cardiopulmonar , Serviços Médicos de Emergência , Parada Cardíaca Extra-Hospitalar , Humanos , Parada Cardíaca Extra-Hospitalar/terapia , Reanimação Cardiopulmonar/métodos , Tempo para o Tratamento
19.
Saudi J Anaesth ; 18(1): 95-99, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38313723

RESUMO

In developing countries, last-mile delivery of medical products is a challenge, especially in hilly and rural areas where there is no road connectivity. As helicopters or other air services are not affordable all the time, drones can be used for the supply of medical products. They are cost-effective as compared to other air or road transport. However, the carrying capacity of drone is less, it is not able to carry heavier payloads. Also, operating drones requires trained operators, and it is a new venture in a developing country so possibilities of confusion and lack of clarity on operating procedures are there. Drones are becoming increasingly reliable for the health care delivery. This narrative review explores the use of drones in healthcare delivery globally.

20.
Life (Basel) ; 14(2)2024 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-38398731

RESUMO

The growth and development of honeybees are influenced by many factors, one of which is the cell size of the brood comb. Larger worker bees can be obtained by being raised in bigger cells. However, whether cell size has the same effect on drone development is still unknown. Here, using 3D-printed foundations, we observed the development of drones kept in comb cells of different sizes from the late larval stage through eclosion. The results showed that drones in larger cell-size combs had heavier body weights, longer body lengths, and larger head widths, thorax widths, and abdomen widths compared to those in smaller cell-size combs. Furthermore, regardless of developmental stages, the drones' body weights increased linearly with the comb's cell size. However, the other morphological changes of drones in different developmental stages were out of proportion to the cell-size changes, resulting in smaller cells with a higher fill factor (thorax width/cell width). Our findings confirm that comb cell size affects the development of honeybees; drones become bigger when raised in large cells.

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