Comprehensive Postnatal Anatomical, Histological and Geometric Morphometric Analysis of Thymus Development in Dromedary Camels (Camelus dromedarius).

The thymus, a primary lymphoid organ, plays a critical role in T lymphocyte development and adaptive immunity. This study focuses on the anatomical, histological and geometric morphometric characteristics of the thymus in dromedary camels (Camelus dromedarius) during postnatal development. Thymus samples were collected from camels aged approximately 4, 8, 12 and 16 months. Using photogrammetry and 3D modelling, the samples were analysed to generate landmarks and conduct geometric morphometry with the 3D Slicer and ALPACA algorithm. Principal component analysis (PCA) was then performed to evaluate shape variations. Histologically, the samples underwent Haematoxylin and Eosin and Masson's trichrome staining. Image analysis using QuPath software quantified trabeculae, adipose tissue and Hassall's corpuscles. The results revealed significant anatomical and histological changes in the thymus across the different age groups. Notable variations in tissue composition and structural integrity were observed, with the PCA highlighting distinct morphometric patterns associated with age-related development. These findings provide a deeper understanding of thymus maturation in dromedaries and offer valuable data for comparative anatomy and veterinary medicine. This comprehensive analysis enhances our knowledge of species-specific immune development, with important implications for the health and resilience of these animals in arid environments.

Geometric morphometric analysis of an ontogenetic cranial series of the Permian dicynodont Diictodon feliceps.

The Karoo Basin of South Africa is renowned for its abundance and diversity of therapsid fossils. Among the most ubiquitous and persistent of the Permian fauna is the small herbivorous dicynodont Diictodon feliceps. Intraspecific variation in Diictodon is historically confounding, and while ontogeny is frequently cited as a potential source of variation, observable developmental changes have never been calibrated. The present study revisits this issue, comparing three-dimensional landmark configurations of 82 Diictodon crania to investigate the association between shape, size and dimorphism. Beyond the statistically significant relationship between shape and allometry, our results determine the shape differences between juvenile and adult skulls of Diictodon, aligned with common craniofacial features documented in other tetrapod taxa. Functionally, these changes are attributed to development of the jaw musculature for feeding on larger, tougher plant matter during later ontogeny. Cranial morphological variation owing to sexual dimorphism is negligible, but distinct differences are noted in the allometric trajectories of each morphotype. A component of non-allometric variation cannot be accounted for, and we propose that this represents natural variation, rather than an artefact of taphonomic deformation.

Using three-dimensional geometric morphometry for facial analysis in patients with the oculo-auriculo-vertebral spectrum.

AIM: To utilize three-dimensional (3D) geometric morphometry for visualization of the level of facial asymmetry in patients with the oculo-auriculo-vertebral spectrum (OAVS). MATERIALS AND METHODS: Three-dimensional facial scans of 25 Czech patients with OAVS were processed. The patients were divided into subgroups according to Pruzansky classification. For 13 of them, second 3D facial scans were obtained. The 3D facial scans were processed using geometric morphometry. Soft tissue facial asymmetry in the sagittal plane and its changes in two time spots were visualized using colour-coded maps with a thermometre-like scale. RESULTS: Individual facial asymmetry was visualized in all patients as well as the mean facial asymmetry for every Pruzansky subgroup. The mean colour-coded maps of type I and type IIA subgroups showed no differences in facial asymmetry, more pronounced asymmetry in the middle and the lower facial third was found between type IIA and type IIB (maximum 1.5 mm) and between type IIB and type III (maximum 2 mm). The degree of intensity facial asymmetry in affected middle and lower facial thirds did not change distinctly during the two time spots in all subgroups. CONCLUSIONS: The 3D geometric morphometry in OAVS patients could be a useful tool for objective facial asymmetry assessment in patients with OAVS. The calculated colour-coded maps are illustrative and useful for clinical evaluation.

Inter- and intra-observer variation in phytolith morphometry.

BACKGROUND: Archaeobotanists and palaeoecologists extensively use geometric morphometrics to identify plant opal phytoliths. Particularly when applied to assemblages of phytoliths from concentrations retrieved from closed contexts, morphometric data from archaeological phytoliths compared with similar data from reference material may allow taxonomic attribution. Observer variation is one aspect of phytolith morphometry that has received little attention but may be an important source of error, and hence cause of potential misidentification of plant remains. SCOPE: To investigate inter- and intra-observer variation in phytolith morphometry, eight researchers (observers) from different laboratories measured 50 samples each from three phytolith morphotypes, Bilobate, Bulliform flabellate and Elongate dendritic, three times, under the auspices of the International Committee for Phytolith Morphometrics (ICPM). METHODS: Data for 17 size and shape variables were collected for each phytolith by manually digitising a phytolith outline (mask) from a photograph, followed by measurement of the mask with open-source morphometric software. KEY RESULTS: Inter-observer variation ranged from 0 to 23% difference from the mean of all observers. Intra-observer variation ranged from 0 to 9% difference from the mean of individual observers per week. Inter- and intra-observer variation was generally higher among inexperienced researchers. CONCLUSIONS: Scaling errors were a major cause of variation and occurred more with less experienced researchers, which is likely related to familiarity with data collection. The results indicate that inter- and intra-observer variation can be substantially reduced by providing clear instructions for and training with the equipment, photo capturing, software, data collection and data cleaning. In this paper, the ICPM provides recommendations to minimise variation.Advances in automatic data collection may eventually reduce inter- and intra-observer variation, but until this is common practice, the ICPM recommends that phytolith morphometric analyses adhere to standardised guidelines to assure that measured phytolith variables are accurate, consistent and comparable between different researchers and laboratories.

Shape analysis of the carpal joint in healthy and septic arthritis in newborn calves.

Septic arthritis is common in newborn calves due to poor birth and housing hygiene. This study investigated the pathological deformities caused by arthritis in the carpal bones of calves using geometric morphometry. The changes in the carpal joint bones of newborn calves with septic arthritis were examined through shape analysis. The study included 20 healthy Simmental calves and 30 Simmental calves with septic arthritis. Dorso-palmar x-ray images of the carpal joint were taken, and geometric morphometry was performed on these images using 25 landmarks. The first principal components (PC1) represented 26.92% of the total variation, while PC2 represented 13.84%. One of the most significant shape changes with increasing PC1 occurred in the os carpi intermedium. The study found that it was statistically possible to discriminate between radiometric carpal joint images of Simmental calves in the control and arthritis groups using geometric morphometry. In newborn calves with septic arthritis, the trochlea radi was located more proximally. There was an enlargement of the os carpi intermedium and a tendency towards the os carpi ulnare in female calves with septic arthritis. These results indicate significant bone deformation due to septic arthritis. Geometric morphometric methods can be clinically useful, as demonstrated in this study. Researchers can statistically explore these shape analyses, opening new avenues for research in this field. This method not only enhances our understanding of morphological changes but also provides a framework for clinical investigations and discoveries in related areas.

Variety is the spice: The role of morphological variation of Triatoma infestans (Hemiptera, Reduviidae) at a macro-scale.

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi (Chagas, 1909). One of the primary vectors of T. cruzi in South America is Triatoma infestans (Klug, 1834). This triatomine species is distributed across a huge latitudinal gradient, inhabiting domiciliary , peridomiciliary , and wild environments. Its wide geographic distribution provides an excellent opportunity to study the relationships between environmental gradients and intraspecific morphological variation. In this study, we investigated variations in wing size and shape in T. infestans across six ecoregions. We aimed to address the following questions: How do wing size and shape vary on a regional scale, does morphological variation follow specific patterns along an environmental or latitudinal gradient, and what environmental factors might contribute to wing variation? Geometric morphometric methods were applied to the wings of 162 females belonging to 21 T. infestans populations, 13 from Argentina (n = 105), 5 from Bolivia (n = 42), and 3 from Paraguay (n = 15). A comparison of wing centroid size across the 21 populations showed significant differences. Canonical Variate Analysis (CVA) revealed significant differences in wing shape between the populations from Argentina, Bolivia, and Paraguay, although there was a considerable overlap, especially among the Argentinian populations. Well-structured populations were observed for the Bolivian and Paraguayan groups. Two analyses were performed to assess the association between wing size and shape, geographic and climatic variables: multiple linear regression analysis (MRA) for size and Partial Least Squares (PLS) regression for shape. The MRA showed a significant general model fit. Six temperature-related variables, one precipitation-related variable, and the latitude showed significant associations with wing size. The PLS analysis revealed a significant correlation between wing shape with latitude, longitude, temperature-related, and rainfall-related variables. Wing size and shape in T. infestans populations varied across geographic distribution. Our findings demonstrate that geographic and climatic variables significantly influence T. infestans wing morphology.

Geometric morphometric anatomy of the lacrimal punctum in normal population: Punctum update (PUP) study - Paper 5.

BACKGROUND: To investigate the geometric morphological differences of the lacrimal punctum by analyzing its shape in relation to age and sex in a normal population. METHODS: 960 high-magnification slit-lamp images were obtained from 320 puncta of normal asymptomatic Indian individuals across eight decades of life. Using advanced geometric morphometric techniques, including Elliptic Fourier Analysis and Principal Component Analysis, the intricate details of the lacrimal punctum's shape in a diverse population sample were categorized by age and sex. High-resolution images of the lacrimal punctum underwent standardization for scale and orientation, followed by precise landmark identification and coordinate data extraction. RESULTS: The geometric morphometry of the lacrimal punctum shows significant changes as one ages. However, the gender differences, in isolation, without consideration of age, remain subtle and are not pronounced. Interestingly, detailed Principal Component scores analysis revealed potential sex- and age-related variations specifically for the left and right lower puncta, which warrant further investigation. These changes could reflect unique aging changes in the proximal lacrimal drainage system. CONCLUSION: The study is a starting point for geometric morphometric analysis of the lacrimal punctum and provides valuable insights into the punctal changes in size, orientation, and overall morphology across different age groups and between sexes. These findings highlight the significance of considering individual age-wise anatomical variations to better understand the lacrimal punctum.

Chewing asymmetry in dogs: Exploring the importance of the fossa masseterica and first molar teeth morphology.

Dogs are animals with strong bite force. This strong bite mechanism has led to significant changes in the skeletal system such as fossa masseterica. It can be thought that one side is used more than the other side in chewing and is related to the preference of using the same side's hand, eye and foot. In the study, directional asymmetry and fluctuating asymmetry, which occurs as a result of chewing asymmetry, were examined on the first molar teeth and the fossa masseterica in 85 dog mandibles including a wide diversity of morphotypes. The association of high PC1 values for directional asymmetry with a pronounced cranial index, as evident in breeds like Pekingese, Pomeranian and Bulldog, indicates a potential evolutionary or selective breeding trend favouring brachycephaly. On the contrary, guardian breeds like the German shepherd and Bernese mountain dog, which typically require strong jaws for their roles, showcased reduced PC1 values, which might be related to their functional morphology. Similarly, the PCA results for the first molar teeth shape variations also highlighted the influence of cranial shape, with boxer dogs displaying notably higher PC1 values. The fluctuating asymmetrical distributions provided valuable insights into individualistic variations. Interestingly, no specific breed distribution trend was observed for these asymmetries, indicating a more individual-based variation rather than breed-based. It is essential to note that while these results provide valuable insights, further studies are required to understand the underlying causes better. Factors like genetic variations, developmental processes, dietary habits and external environmental factors could play pivotal roles in these observed morphological differences.

Taxonomic reassessment of Rhodnius zeledoni Jurberg, Rocha & Galvão: a morphological and morphometric analysis comparing its taxonomic relationship with Rhodnius domesticus Neiva & Pinto.

BACKGROUND: Rhodnius zeledoni was described from a single specimen. Since its description, doubts have arisen regarding the taxonomic status of this species in relation to Rhodnius domesticus. METHODS: The present study reviewed and compared R. zeledoni with R. domesticus based on morphological analysis and head geometric morphometrics. RESULTS: Our analysis revealed the absence of distinctive diagnostic characters between the two species at specific levels. Rhodnius zeledoni and R. domesticus show morphological and morphometric similarity, with only minor differences in coloration observed between them. Contrary to previous statements, our analysis showed that R. zeledoni and R. paraensis are not closely related species, not corroborating previous studies with such an assumption. CONCLUSIONS: Therefore, we formally propose R. zeledoni as a junior synonym of R. domesticus.

Relationship between genetic diversity and morpho-functional characteristics of flight-related traits in Triatoma garciabesi (Hemiptera: Reduviidae).

BACKGROUND: Triatoma garciabesi, a potential vector of the parasitic protozoan Trypanosoma cruzi, which is the causative agent of Chagas disease, is common in peridomestic and wild environments and found throughout northwestern and central Argentina, western Paraguay and the Bolivian Chaco. Genetic differentiation of a species across its range can help to understand dispersal patterns and connectivity between habitats. Dispersal by flight is considered to be the main active dispersal strategy used by triatomines. In particular, the morphological structure of the hemelytra is associated with their function. The aim of this study was to understand how genetic diversity is structured, how morphological variation of dispersal-related traits varies with genetic diversity and how the morphological characteristics of dispersal-related traits may explain the current distribution of genetic lineages in this species. METHODS: Males from 24 populations of T. garciabesi across its distribution range were examined. The cytochrome c oxidase I gene (coI) was used for genetic diversity analyses. A geometric morphometric method based on landmarks was used for morpho-functional analysis of the hemelytra. Centroid size (CS) and shape of the forewing, and contour of both parts of the forewing, the head and the pronotum were characterised. Length and area of the forewing were measured to estimate the aspect ratio. RESULTS: The morphometric and phylogenetic analysis identified two distinct lineages, namely the Eastern and Western lineages, which coincide with different ecological regions. The Eastern lineage is found exclusively in the eastern region of Argentina (Chaco and Formosa provinces), whereas the Western lineage is prevalent in the rest of the geographical range of the species. CS, shape and aspect ratio of the hemelytra differed between lineages. The stiff portion of the forewing was more developed in the Eastern lineage. The shape of both portions of the hemelytra were significantly different between lineages, and the shape of the head and pronotum differed between lineages. CONCLUSIONS: The results provide preliminary insights into the evolution and diversification of T. garciabesi. Variation in the forewing, pronotum and head is congruent with genetic divergence. Consistent with genetic divergence, morphometry variation was clustered according to lineages, with congruent variation in the size and shape of the forewing, pronotum and head.

Shape and Size Variations of Distal Phalanges in Cattle.

Studies on the structure of the distal phalanx help explain the development of laminitis. Additionally, examining the structure of the distal phalanx from a taxonomic perspective also contributes to veterinary anatomy. In this study, we examined shape variation in the medial and lateral distal phalanx of both fore- and hindlimbs using the geometric morphometry method. We investigated whether the shape of the distal phalanx differed between phalanx positions and how much of the shape variation in this bone depends on size. For this purpose, distal phalanges from 20 Holstein cattle were used, and the bones were digitized in 3D. A draft containing 176 semi-landmarks was prepared for shape analysis, and this draft was applied to all samples using automated landmarking through point cloud alignment and correspondence analysis. A principal component analysis was performed to obtain general patterns of morphological variation. The centroid size (CS) was employed as an approximation of size. Although distal phalanx groups generally showed close variations, PC1 statistically separated the hindlimb lateral distal phalanx (HL) and the forelimb medial distal phalanx (FM) from each other in shape. While PC2 separated HL from other distal phalanx groups, PC3 separated fore- and hindlimb groups. The shape (Procrustes distance) of the hindlimb medial distal phalanx (HM) is markedly less variable than the other three phalanges. The smallest distal phalanx in size was HL. For both forelimb and hindlimb, the medial distal phalanges were larger than the lateral ones. Size (CS) was found to have an effect on PC1 and PC3. In this study, a reference model of the same breeds for distal phalanx was created. These results can provide useful information, especially in terms of veterinary anatomy, zooarchaeology, and paleontology.

Determination of skull variation in Akkaraman and Kangal Akkaraman sheep by geometric morphometric method.

Sheep breeding has an important place in the world and in Turkey, as sheep are farm animals that provide most of their nutritional needs from pasture, have good adaptability and are low in cost. Akkaraman sheep and the Kangal Akkaraman sheep, which was considered its sub-variety until 2012, constitute half of the sheep population in Turkey. In this study, morphometric diversity between the skulls of Akkaraman and Kangal Akkaraman breed sheep was examined by geometric morphometric method. 2D images obtained from 3D models of 24 male sheep skulls from two different provinces were analyzed using 10 landmarks from dorsal and lateral views. The results from the dorsal view, the first three principal components (PC1, PC2 and PC3) accounted for a total of 64.13% of the morphological variation and the largest changes in are associated with external occipital protuberance, midline nasofrontal suture and rostral edge of the incisive fissure. From the lateral view, three first principal components explained the morphological variation 27.56%, 22.32% and 10.65% respectively. In the comparison of the skull morphology of Akkaraman and Kangal Akkaraman sheep from dorsal and lateral views, no allometric components were identified, as most of the described shape variations were not size-dependent. As a result of these analyses, more detailed information was obtained about the shape differences of the skull. Geometric morphometric analysis is successful in detecting differences in skull shape of different sheep breeds and can be successfully used for classification.

Orbital shape in sheep and goats with sex and breed factors.

The purpose of this study was to identify the dimorphic structures of the orbita based on breed and sex factors through shape analysis across species. Additionally, the study aimed to ascertain the variability between the two species. A total of 86 (51 sheep-Akkaraman [Ak] and Morkaraman [Mk], 35 goats-Hair [Hr] and Honamli [Hm]) skulls were used in the study. It was decided to designate 36 landmarks to represent the orbit's edge. In the principal component analysis, 68 PCs were calculated. It was determined that PC1, PC2 and PC3 explained the total variance in morphology by 26.909%, 16.147% and 13.616%, respectively. According to the cross-validation results, the goats were grouped with 95% (Hm: 100%, Hr: 90%), while the sheep were grouped with 56% (Ak: 50%, Mk: 63%). Therefore, dimorphism among goat breeds on orbita shape was more pronounced compared to sheep. In the discriminant function analysis of the sex groups, Procrustes and Mahalanobis distance values in goats were 0.04425425 (p < 0.0001) and 5.3733 (p = 0.9550), in sheep 0.03513424 (p < 0.0001) and 7.1003 (p = 0.9414), respectively, was detected. According to the cross-validation results, in terms of sex, goats were correctly grouped at a rate of 74% (F: 73%; M: 75%) and sheep breeds were correctly grouped at a rate of 72% (F: 77%; M: 66%). Factors such as habitat selection, breeding behaviours and ecological environment may show anatomical differences within breeds as well as between breeds. Therefore, various measurement techniques are used to reveal these differences. As a result, it is thought that the study will contribute to many disciplines, especially taxonomy and zooarchaeology, by analysing the shape of the orbita of sheep and goat breeds, the domestication of which dates back to ancient times, and revealing the similarities and differences between species and according to sex.

Reed bamboos drive skull shape evolution in bush frogs of the Western Ghats, Peninsular India.

Reed bamboo is a major ecological and economic resource for many animals, including humans. Nonetheless, the influence of this plant's evolutionary role on the morphology of animal species remains unexplored. Here, we investigated the significance of bamboo habitats as ecological opportunities in shaping the skull morphology of bush frogs (Raorchestes) from the Western Ghats, Peninsular India. We applied a three-dimensional (3D) geometric morphometric approach to capture the skull shape of 55 species of bush frogs. We visualized the skull shape variables in phylomorphospace with principal component analysis and performed phylogenetic generalized least-squares analysis to assess the impact of cranial size (evolutionary allometry) and habitat (bamboo or non-bamboo) on cranial shape. We quantified the morphological disparity between bamboo and non-bamboo bush frogs' skull shape, and employed RRphylo, a phylogenetic ridge regression method, to access the evolutionary rate and rate shifts of skull shape change. The phylomorphospace delineated bamboo and non-bamboo bush frogs. While cranial shape exhibited a significant but smaller association with size, its association with habitat type was non-significant. We detected, however, significant differences in skull shape between the two frog groups, with bamboo frogs showing higher morphological disparity and a remarkable shift in the evolutionary rate of skull shape diversification. These findings underscore the role of reed bamboo in the evolution of skull shape in the radiation of frogs, endemic to the Western Ghats. We demonstrate that the association between the members of two distinct endemic clades (bamboo reeds and bamboo frogs) is the outcome of a deep-time ecological opportunity that dates back to the Miocene.

In Search of Apis mellifera pomonella in Kazakhstan.

Apis mellifera pomonella is one of two subspecies that represent the eastern limits of honey bee (Apis mellifera) distribution, and it is important to understand its biogeography and evolution. Despite this, A. m. pomonella was not investigated after its discovery 20 years ago. In particular, it is not known if it was hybridized or not with exotic subspecies introduced by beekeepers. In this study, we analysed the variation in honey bee forewing shape in Kazakhstan. Bees were collected from apiaries, where the origin of the queens was not controlled by beekeepers; they formed a group called "local bees", and apiaries where queens declared as either A. m. carnica or A. m. mellifera were obtained from queen breeders. The two later groups were called "exotic bees". We showed that local bees are still distinct from exotic ones. However, some samples showed signs of introgression with exotic subspecies from lineage C. In terms of wing shape, the local bees were most similar to lineage O. We concluded that the local bees most likely represented A. m. pomonella. We provided wing images and identification data, which can help to identify A. m. pomonella and protect it in the future. It is interesting that the nearby honey bee population sourced from China, which is not separated by any clear barrier to gene flow, belonged to lineage M.

Shape analysis of fossa masseterica and processus coronoideus in domestic cats (Felis catus) and domestic dogs (Canis familiaris).

Fossa masseterica, to which the musculus masseter is attached, is the pit on the lateral surface of the ramus mandibulae. Processus coronoideus is a protrusion on the upper part of fossa masseterica. Carnivores have a more developed fossa masseterica and a wider processus coronoideus than other species, due to their well-developed jaw muscles. However, there is limited information about the differences of these two structures among carnivorous species. In this study, it was investigated whether fossa masseterica and processus coronoideus differ in shape in domestic cats and domestic dogs. For this purpose, 22 dogs and 20 cats were examined by 3D geometric morphometry. Eighty-one landmarks were used on the fossa masseterica and processus coronoideus. The difference in centroid sizes and shape for cats and dogs was statistically significant (p < 0.0001). PC1 explained 26.47% of the total variance. It was seen that cats and dogs were completely separated according to PC1 results. In cats with a high PC1 value, the processus coronoideus was narrower than in dogs. Also, feline processus coronoideus was more curved than domestic canine processus coronoideus. In addition, the caudal inclination of processus coronoideus was deeper in dogs than in cats. Dogs had a negative PC1 value except for one sample (German Shepherd). The sample with the lowest PC1 value was the French Bulldog (female, 7 years, and 13 kg). Discriminant analysis results showed that the domestic cats and domestic dogs used in the study were completely separated from each other, and this difference was statistically significant. The results of this study showed that dogs with stronger jaw muscles had a deeper fossa masseterica and a wider processus coronoideus than cats.

Resistance to deltamethrin in Triatoma infestans (Hemiptera: Reduviidae): Does it influence the phenotype of antennae, wings, and heads?

In vector control terms, insecticide resistance is the development of the capacity, of an insect population, to tolerate doses of an insecticide that are lethal to most individuals in a typical population of the same species. The genetic changes that determine resistance may have adaptive costs in the resistant phenotype or, conversely, may result in an adaptive advantage when compared to susceptible insects in the environment without insecticides. Triatoma infestans is one of the main vectors of Trypanosoma cruzi in the southern cone of South America. High insecticide resistance in T. infestans was detected in Argentina in Salta and Chaco provinces. The objective of this study was to determine the possible morphometric changes in wings, heads, and the antennal phenotype of deltamethrin-resistant T. infestans (RR) males and females compared to susceptible insects (SS), evaluating its implication in adaptive processes such as olfactory capacity, dispersion, and probability of colonizing new habitats, among others. Nine type I landmarks were marked on wings, 5 type II landmarks on heads, and 10 antennal sensilla were counted on 106 adults of both sexes (resistant and susceptible from first and second laboratory generations). Morphological divergence was observed between the two groups (RR and SS). The RR insects showed smaller sizes of wings and heads and shape compatible with lower dispersal potential and different active dispersal behaviors. Antennae also revealed sensory simplification in RR and divergence between RR and SS, although more marked in females. This study characterizes for the first time T. infestans RR and SS through wings, heads, and antennae. The results suggest a lower dispersive potential in resistant insects and the differences described lay the foundations for the identification of a resistance biomarker in triatomines.

Qualitative Histologic Assessment vs. Geomorphometric Analysis of Nerve Fiber Shape after the Intraneural Application of Liposomal Bupivacaine / Evaluación Histológica Cualitativa Versus Análisis Geomorfométrico de la Forma de la Fibra Nerviosa Después de la Aplicación Intraneural de Bupivacaína Liposomal

SUMMARY: The preserved form of all components of the nerve fiber is a prerequisite for the proper conduction of the nerve impulse. various factors can change the shape of nerve fibers. In everyday practice, qualitative histological analysis is the gold standard for detecting changes in shape. Geometric morphometry is an innovative method that objectively enables the assessment of changes in nerve fibers' shape after local anesthetics action. A total of sixty sciatic nerves were used as material, which was intraneural injected with saline solution in the control group (n=30), and a solution of 1.33 % liposomal bupivacaine (n=30) in the test group. After the animals were sacrificed, nerve samples were taken and histological preparations were made. The preparations were first described and examined using a qualitative histological method, after which digital images were made. The images were entered into the MorphoJ program and processed using the method of geometric morphometry. Qualitative histological examination revealed no differences in nerve fibers after intraneurally applied physiological solution and liposomal bupivacaine. Using the method of geometric morphometry, a statistically significant change in the shape of axons was found after intraneurally applied saline solution and liposomal bupivacaine (p=0.0059). No significant differences in histological changes were found after the qualitative histological analysis of nerve fiber cross-section preparations. A statistically significant change in the shape of nerve fiber axons was observed after geometric morphometric analysis of digital images after intraneural application of saline and liposomal bupivacaine.

La forma conservada de todos los componentes de la fibra nerviosa es un requisito previo para la conducción correcta del impulso nervioso. Varios factores pueden cambiar la forma de las fibras nerviosas. En la práctica diaria, el análisis histológico cualitativo es el estándar de oro para detectar cambios de forma. La morfometría geométrica es un método innovador que permite evaluar objetivamente los cambios en la forma de las fibras nerviosas después de la acción de los anestésicos locales. Se utilizó como material un total de sesenta nervios ciáticos, que se inyectaron intraneuralmente con solución salina en el grupo control (n=30), y una solución de bupivacaína liposomal al 1,33 % (n=30) en el grupo de prueba. Después de sacrificados los animales, se tomaron muestras de nervios y se realizaron preparaciones histológicas. Primero se describieron y examinaron las preparaciones utilizando un método histológico cualitativo, después de lo cual se tomaron imágenes digitales. Las imágenes fueron ingresadas al programa MorphoJ y procesadas mediante el método de morfometría geométrica. El examen histológico cualitativo no reveló diferencias en las fibras nerviosas después de la aplicación intraneural de solución fisiológica y bupivacaína liposomal. Usando el método de morfometría geométrica, se encontró un cambio estadísticamente significativo en la forma de los axones después de la aplicación intraneural de solución salina y bupivacaína liposomal (p = 0,0059). No se encontraron diferencias significativas en los cambios histológicos después del análisis histológico cualitativo de las preparaciones de secciones transversales de fibras nerviosas. Se observó un cambio estadísticamente significativo en la forma de los axones de las fibras nerviosas después del análisis de morfometría geométrica de imágenes digitales después de la aplicación intraneural de solución salina y bupivacaína liposomal.

Triatoma yelapensis sp. nov. (Hemiptera: Reduviidae) from Mexico, with a Key of Triatoma Species Recorded in Mexico.

Thirty-four species of Triatominae (Hemiptera, Reduviidae) are recorded in Mexico, Triatoma Laporte, 1832 the most speciose genus in this country. Here, we describe Triatoma yelapensis sp. nov. from the Pacific coast of Jalisco (Mexico). The most similar species to T. yelapensis sp. nov. is T. recurva (Stål, 1868), but they differ in head longitude, the proportion of labial segments, coloration pattern of corium and connexivum, spiracles location, and male genitalia. To provide statistical support for the morphological distinctiveness of the new species, we performed a geometric morphometric analysis of T. yelapensis sp. nov., T. dimidiata s.s. (Latreille, 1811), T. gerstaeckeri (Stål, 1859), and T. recurva (Stål, 1868), considering head morphology. We also provide an updated key of the genus Triatoma for species recorded in Mexico.

Skull of the Asian (Paradoxurus Hermaphroditus) and the golden (Paradoxurus Zeylonensis) palm civet: Geometric morphometric analysis using palate, tooth and frontal landmarks.

This study aims to determine the differences in skull between Asian and golden palm civets by geometric morphometric method. The landmarks were marked on these photographs, and principal component, classical cluster and canonical variance analyses were performed. The method of geometric morphometry of the skull is a powerful tool used to differentiate the species. Fourteen and 20 homologous landmarks were marked using tpsutil and tpsdig2 programs on the frontal and palatinal directional photographs, respectively. As a result of the study, the first principal component (PC1) accounted for 33.586% on the frontal view, 41.602% on the palatinal view of the total shape differences, respectively. Distinctive differences on the frontal and palatinal view were observed in the caudal, rostrolateral and the last molars and the caudal palatine, respectively.