Aquatic processing enhances the loss of aged carbon from drained and burned peatlands.

Water-logged peatlands store tremendous amounts of soil carbon (C) globally, accumulating C over millennia. As peatlands become disturbed by human activity, these long-term C stores are getting destabilized and ultimately released as greenhouse gases that may exacerbate climate change. Oxidation of the dissolved organic carbon (DOC) mobilized from disturbed soils to streams and canals may be one avenue for the transfer of previously stored, millennia-aged C to the atmosphere. However, it remains unknown whether aged peat-derived DOC undergoes oxidation to carbon dioxide (CO2) following disturbance. Here, we use a new approach to measure the radiocarbon content of CO2 produced from the oxidation of DOC in canals overlying peatland soils that have undergone widespread disturbance in Indonesia. This work shows for the first time that aged DOC mobilized from drained and burned peatland soils is susceptible to oxidation by both microbial respiration and photomineralization over aquatic travel times for DOC. The bulk radiocarbon age of CO2 produced during canal oxidation ranged from modern to ~1300 years before present. These ages for CO2 were most strongly influenced by canal water depth, which was proportional to the water table level where DOC is mobilized from disturbed soils to canals. Canal microbes preferentially respired older or younger organic C pools to CO2, and this may have been facilitated by the use of a small particulate organic C pool over the dissolved pool. Given that high densities of canals are generally associated with lower water tables and higher fire risk, our findings suggest that peatland areas with high canal density may be a hotspot for the loss of aged C on the landscape. Taken together, the results of this study show how and why aquatic processing of organic C on the landscape can enhance the transfer of long-term peat C stores to the atmosphere following disturbance.

Lignite reduces carbon and nitrogen loss from litter in commercial broiler housing.

The loss of carbon and nitrogen from broiler litter limits nutrient recycling and is damaging to the environment. This study investigated lignite, a low-rank brown coal, as an amendment to reduce the loss of carbon and nitrogen from broiler litter over 3 consecutive grow-out cycles, November 2021 to May 2022, at a commercially operated farm in Victoria, Australia. Lignite-treated litter contained significantly more carbon and nitrogen, with an increase of 70.1 g/bird and 12.6 g/bird for carbon and nitrogen, respectively. Lignite also reduced aerobic microbial respiration, with a 46.0% reduction in CO2 flux recorded in week 7 of the study, resulting in reduced mass loss. It is expected that this is a key mechanism responsible for nutrient retention in litter following treatment with lignite. Furthermore, lignite treatment lowered litter moisture content by 7, 6 and 3 percentage points for grow-out 1, 2 and 3, respectively. These findings present lignite as a beneficial litter amendment for increasing the nutrient value of waste and reducing carbon dioxide emissions. The study highlights the potential of lignite to reduce the environmental impact of poultry production and presents an alternative use for lignite as an existing resource.

Enhancing biodegradation of aged hydrocarbon-contaminated soils through toluene addition: assessing effects on solid and slurry phase treatments.

The main challenge in treating aged soils highly contaminated with total petroleum hydrocarbons (TPH) is to enhance their bioavailability for microbial degradation. Hydrocarbons in soils undergo chemical changes that make them more resistant to biodegradation. This study investigates toluene's efficacy in enhancing the biodegradation of aged hydrocarbon-contaminated soil containing 292,000 mg TPH kg-1 dry soil. Toluene's effect was compared between solid phase (SOP) and slurry phase (SLP) treatments using a microbial consortium isolated from Cyperus laxus rhizosphere. TPH biodegradation and microbial respiration were measured, the latter to estimate the respiratory quotient (RQ, the ratio between moles of carbon dioxide released and moles of oxygen absorbed during respiration). Toluene significantly accelerated TPH biodegradation in both treatments, achieving ~ 30% higher removal than in a non-solvent control, possibly through improved bioavailability of aromatic compounds and other low molecular weight compounds. According to the RQ analysis, toluene enhanced microbial respiratory processes and hydrocarbon catabolism with higher hydrocarbon mineralization (RQ = ~ 0.5) in both SOP and SLP assays. Our results reveal toluene's potential to increase hydrocarbon availability and microbial degradation efficiency in aged contaminated soils; its use in various bioremediation techniques could be of broad applicability across diverse soil types and pollutants.

In situ simultaneous measuring method for the determination of key processes of soil organic carbon cycling: Soil microbial respiration using laser spectrometry.

RATIONALE: Soil microbial heterotrophic C-CO2 respiration is important for C cycling. Soil CO2 differentiation and quantification are vital for understanding soil C cycling and CO2 emission mitigation. Presently, soil microbial respiration (SR) quantification models are based on native soil organic matter (SOM) and require consistent monitoring of δ13C and CO2. METHODS: We present a new apparatus for achieving in situ soil static chamber incubation and simultaneous CO2 and δ13C monitoring by cavity ring-down spectroscopy (CRDS) coupled with a soil culture and gas introduction module (SCGIM) with multi-channel. After a meticulous five-point inter-calibration, the repeatability of CO2 and δ13C values by using CRDS-SCGIM were determined, and compared with those obtained using gas chromatography (GC) and isotope ratio mass spectrometry (IRMS), respectively. We examined the method regarding quantifying SR with various concentrations and enrichment of glucose and then applied it to investigate the responses of SR to the addition of different exogenous organic materials (glucose and rice residues) into paddy soils during a 21-day incubation. RESULTS: The CRDS-SCGIM CO2 and δ13C measurements were conducted with high precision (< 1.0 µmol/mol and 1‰, respectively). The optimal sampling interval and the amount added were not exceeded 4 h and 200 mg C/100 g dry soil in a 1 L incubation bottle, respectively; the 13C-enrichment of 3%-7% was appropriate. The total SR rates observed were 0.6-4.2 µL/h/g and the exogenous organic materials induced -49%-28% of priming effects in native SOM mineralisation. CONCLUSIONS: Our results show that CRDS-SCGIM is a method suitable for the quantification of soil microbial CO2 respiration, requiring less extensive lab resources than GC/IRMS.

Ecotoxicological impact of butisanstar and clopyralid herbicides on soil microbial respiration and the enzymatic activities.

The application of herbicides in soil has been noted for its detrimental effect on the soil microbial community, crucial for various biochemical processes. This study provides a comprehensive assessment of the impact of butisanstar and clopyralid herbicides, both individually and in combination at different dosage (recommended field dose (RFD), ½, 2 and 5-times RFD). The assessment focuses on soil basal respiration (SBR), cumulative microbial respiration (CMR), and the activities dehydrogenase (DH), catalase (CAT), urease, acid and alkaline phosphatases (Ac-P and Alk-P) enzymes, along with their variations on days 10, 30, 60, and 90 post-herbicide application. Results indicate that, although herbicides, even at lower doses of RFD, demonstrate inhibitory effects on DH, CAT, and microbial respiration, they paradoxically lead to a significant enhancement in urease and phosphatase activities, even at higher doses. The inhibitory/enhancing intensity varies based on herbicide type, incubation period, and dosage. Co-application of herbicides manifests synergistic effects compared to individual applications. The most notable inhibitory effects on DH, CAT, and SBR are observed on the 30th day, coinciding with the highest activities of urease and phosphatases on the same day. The persistent inability to restore respiration and enzyme activities to initial soil (control) levels emphasizes the lasting adverse and inhibitory effects of herbicides, especially clopyralid, over the long term. It becomes apparent that soil microorganisms require an extended duration to decompose and acclimate to the presence of herbicides. Consequently, these agrochemical compounds pose a potential risk to crucial biochemical processes, such as nutrient cycling, ultimately impacting crop production.

Stronger compensatory thermal adaptation of soil microbial respiration with higher substrate availability.

Ongoing global warming is expected to augment soil respiration by increasing the microbial activity, driving self-reinforcing feedback to climate change. However, the compensatory thermal adaptation of soil microorganisms and substrate depletion may weaken the effects of rising temperature on soil respiration. To test this hypothesis, we collected soils along a large-scale forest transect in eastern China spanning a natural temperature gradient, and we incubated the soils at different temperatures with or without substrate addition. We combined the exponential thermal response function and a data-driven model to study the interaction effect of thermal adaptation and substrate availability on microbial respiration and compared our results to those from two additional continental and global independent datasets. Modeled results suggested that the effect of thermal adaptation on microbial respiration was greater in areas with higher mean annual temperatures, which is consistent with the compensatory response to warming. In addition, the effect of thermal adaptation on microbial respiration was greater under substrate addition than under substrate depletion, which was also true for the independent datasets reanalyzed using our approach. Our results indicate that thermal adaptation in warmer regions could exert a more pronounced negative impact on microbial respiration when the substrate availability is abundant. These findings improve the body of knowledge on how substrate availability influences the soil microbial community-temperature interactions, which could improve estimates of projected soil carbon losses to the atmosphere through respiration.

Microbial-driven mechanisms for the effects of heavy metals on soil organic carbon storage: A global analysis.

Heavy metal (HM) enrichment is closely related to soil organic carbon (SOC) pools in terrestrial ecosystems, which are deeply intertwined with soil microbial processes. However, the influence of HMs on SOC remains contentious in terms of magnitude and direction. A global analysis of 155 publications was conducted to integrate the synergistic responses of SOC and microorganisms to HM enrichment. A significant increase of 13.6 % in SOC content was observed in soils exposed to HMs. The response of SOC to HMs primarily depends on soil properties and habitat conditions, particularly the initial SOC content, mean annual precipitation (MAP), initial soil pH, and mean annual temperature (MAT). The presence of HMs resulted in significant decreases in the activities of key soil enzymes, including 31.9 % for soil dehydrogenase, 24.8 % for ß-glucosidase, 35.8 % for invertase, and 24.3 % for cellulose. HMs also exerted inhibitory effects on microbial biomass carbon (MBC) (26.6 %), microbial respiration (MR) (19.7 %), and the bacterial Shannon index (3.13 %) but elevated the microbial metabolic quotient (qCO2) (20.6 %). The HM enrichment-induced changes in SOC exhibited positive correlations with the response of MBC (r = 0.70, p < 0.01) and qCO2 (r = 0.50, p < 0.01), while it was negatively associated with ß-glucosidase activity (r = 0.72, p < 0.01) and MR (r = 0.39, p < 0.01). These findings suggest that the increase in SOC storage is mainly attributable to the inhibition of soil enzymes and microorganisms under HM enrichment. Overall, this meta-analysis highlights the habitat-dependent responses of SOC to HM enrichment and provides a comprehensive evaluation of soil carbon dynamics in an HM-rich environment.

Slow closure of Earth's carbon cycle.

Carbon near the Earth's surface cycles between the production and consumption of organic carbon; the former sequesters carbon dioxide while the latter releases it. Microbes attempt to close the loop, but the longer organic matter survives, the slower microbial degradation becomes. This aging effect leaves observable quantitative signatures: Organic matter decays at rates that are inversely proportional to its age, while microbial populations and concentrations of organic carbon in ocean sediments decrease at distinct powers of age. Yet mechanisms that predict this collective organization remain unknown. Here, I show that these and other observations follow from the assumption that the decay of organic matter is limited by progressively rare extreme fluctuations in the energy available to microbes for decomposition. The theory successfully predicts not only observed scaling exponents but also a previously unobserved scaling regime that emerges when microbes subsist on the minimum energy flux required for survival. The resulting picture suggests that the carbon cycle's age-dependent dynamics are analogous to the slow approach to equilibrium in disordered systems. The impact of these slow dynamics is profound: They preclude complete oxidation of organic carbon in sediments, thereby freeing molecular oxygen to accumulate in the atmosphere.

Subarctic winter warming promotes soil microbial resilience to freeze-thaw cycles and enhances the microbial carbon use efficiency.

Climate change is predicted to cause milder winters and thus exacerbate soil freeze-thaw perturbations in the subarctic, recasting the environmental challenges that soil microorganisms need to endure. Historical exposure to environmental stressors can facilitate the microbial resilience to new cycles of that same stress. However, whether and how such microbial memory or stress legacy can modulate microbial responses to cycles of frost remains untested. Here, we conducted an in situ field experiment in a subarctic birch forest, where winter warming resulted in a substantial increase in the number and intensity of freeze-thaw events. After one season of winter warming, which raised mean surface and soil (-8 cm) temperatures by 2.9 and 1.4°C, respectively, we investigated whether the in situ warming-induced increase in frost cycles improved soil microbial resilience to an experimental freeze-thaw perturbation. We found that the resilience of microbial growth was enhanced in the winter warmed soil, which was associated with community differences across treatments. We also found that winter warming enhanced the resilience of bacteria more than fungi. In contrast, the respiration response to freeze-thaw was not affected by a legacy of winter warming. This translated into an enhanced microbial carbon-use efficiency in the winter warming treatments, which could promote the stabilization of soil carbon during such perturbations. Together, these findings highlight the importance of climate history in shaping current and future dynamics of soil microbial functioning to perturbations associated with climate change, with important implications for understanding the potential consequences on microbial-mediated biogeochemical cycles.

Heat wave-induced microbial thermal trait adaptation and its reversal in the Subarctic.

Climate change predictions suggest that arctic and subarctic ecosystems will be particularly affected by rising temperatures and extreme weather events, including severe heat waves. Temperature is one of the most important environmental factors controlling and regulating microbial decomposition in soils; therefore, it is critical to understand its impact on soil microorganisms and their feedback to climate warming. We conducted a warming experiment in a subarctic birch forest in North Sweden to test the effects of summer heat waves on the thermal trait distributions that define the temperature dependences for microbial growth and respiration. We also determined the microbial temperature dependences 10 and 12 months after the heat wave simulation had ended to investigate the persistence of the thermal trait shifts. As a result of warming, the bacterial growth temperature dependence shifted to become warm-adapted, with a similar trend for fungal growth. For respiration, there was no shift in the temperature dependence. The shifts in thermal traits were not accompanied by changes in α- or ß-diversity of the microbial community. Warming increased the fungal-to-bacterial growth ratio by 33% and decreased the microbial carbon use efficiency by 35%, and both these effects were caused by the reduction in moisture the warming treatments caused, while there was no evidence that substrate depletion had altered microbial processes. The warm-shifted bacterial thermal traits were partially restored within one winter but only fully recovered to match ambient conditions after 1 year. To conclude, a summer heat wave in the Subarctic resulted in (i) shifts in microbial thermal trait distributions; (ii) lower microbial process rates caused by decreased moisture, not substrate depletion; and (iii) no detectable link between the microbial thermal trait shifts and community composition changes.

Co-culture between Miscanthus x giganteus and Trifolium repens L. to enhance microbial activity, biomass and density in a PAH contaminated technosol.

Phytoremediation is a biological soil remediation technique using plants and their associated microorganisms to clean-up contaminated soils and improve soils' quality. We tested whether a co-culture between Miscanthus x giganteus (MxG) and Trifolium repens L. would enhance the soil biological quality. The objective was to determine the influence of MxG in mono- and in co-culture with white clover on the soil microbial activity, biomass and density. MxG was tested in mono- and in co-culture with white clover in a mesocosm over 148 days. The microbial respiration (CO2 production), the microbial biomass and the microbial density of the technosol were measured. Results showed that MxG induced an increase in microbial activity in the technosol compared to the non-planted condition with the co-culture having a greater impact. Regarding the bacterial density, MxG in mono- and in co-culture significantly increased the 16S rDNA gene copy number. The co-culture increased the microbial biomass, the fungal density and stimulated the degrading bacterial population, contrary to the monoculture and the non-planted condition. We can conclude the co-culture between MxG and white clover was more interesting than MxG monoculture in regards to the technosol biological quality and its potential for PAH remediation improvement.

Our precedent results have shown the benefits of using Miscanthus x giganteus in association with Trifolium repens L. to improve polycyclic aromatic hydrocarbons dissipation and decrease soil ecotoxicity compared to monocultures. In this study we focused on the plant species' influence on the soil's biological quality to improve MxG biomass productivity in the long term and phytoremediation. Many bioindicators were used such as microbial activity, microbial biomass as well as bacteria, fungi and PAH-degrading bacteria density.We showed it was more beneficial to use co-culture instead of MxG monoculture to improve biological technosol quality and in particular microbial activity and biomass as well as fungi and PAH-degrading bacteria density.

The role of edaphic variables and management practices in regulating soil microbial resilience to drought - A meta-analysis.

Environmental disturbances such as drought can impact soil health and the resistance (ability to withstand environmental stress) and resilience (ability to recover functional and structural integrity after stress) of soil microbial functional activities. A paucity of information exists on the impact of drought on soil microbiome and how soil biological systems respond to and demonstrate resilience to drought stress. To address this, we conducted a systematic review and meta-analysis (using only laboratory studies) to assess the response of soil microbial biomass and respiration to drought stress across agriculture, forest, and grassland ecosystems. The meta-analysis revealed an overall negative response of microbial biomass in resistance (-31.6 %) and resilience (-0.3 %) to drought, suggesting a decrease in soil microbial biomass content. Soil microbial respiration also showed a negative response in resistance to drought stress indicating a decrease in soil microbial respiration in agriculture (-17.5 %), forest (-64.0 %), and grassland (-65.5 %) ecosystems. However, it showed a positive response in resilience to drought, suggesting an effective recovery in microbial respiration post-drought. Soil organic carbon (SOC), clay content, and pH were the main regulating factors of the responses of soil microbial biomass and respiration to drought. In agriculture ecosystem, soil pH was primarily correlated with soil microbial respiration resistance and resilience to drought, potentially influenced by frequent land preparation and fertilizer applications, while in forest ecosystem SOC, clay content, and pH significantly impacted microbial biomass and respiration resistance and resilience. In grassland ecosystem, SOC was strongly associated with biomass resilience to drought. The impact of drought stress on soil microbiome showed different patterns in natural and agriculture ecosystems, and the magnitude of microbial functional responses regulated by soil intrinsic properties. This study highlighted the importance of understanding the role of soil properties in shaping microbial responses to drought stress for better ecosystem management.

Impact of redox fluctuations on microbe-mediated elemental sulfur disproportionation and coupled redox cycling of iron.

Elemental sulfur (S0) plays a vital role in the coupled cycling of sulfur and iron, which in turn affects the transformation of carbon and various pollutants. These processes have been well characterized under static anoxic or oxic conditions, however, how the natural redox fluctuations affect the bio-mediated sulfur cycling and coupled iron cycling remain enigmatic. The present work examined S0 disproportionation as driven by natural microbial communities under fluctuating redox conditions and the contribution of S0 disproportionation to ferrihydrite transformation. Samples were incubated at either neutral or alkaline pH values, applying sequential anaerobic, aerobic and anaerobic conditions over 60 days. Under anaerobic conditions, S0 was found to undergo disproportionation to sulfate and sulfide, which subsequently reduced ferrihydrite at both pH 7.4 and 9.5. Ferrihydrite promoted S0 disproportionation by scavenging biogenic sulfide and maintaining a suitable degree of sulfate formation. After an oxic period, during the subsequent anoxic incubation, bioreduction of sulfate occurred and the biogenic sulfide reduced iron (hydr)oxides at a rate approximately 25 % lower than that observed during the former anoxic period. A 16S rDNA-based microbial community analysis revealed changes in the microbial community in response to the redox fluctuations, implying an intimate association with the coupled cycling of sulfur and iron. Microscopic and spectroscopic analyses confirmed the S0-mediated transformation of ferrihydrite to crystalline iron (hydr)oxide minerals such as lepidocrocite and magnetite and the formation of iron sulfides precipitated under fluctuating redox conditions. Finally, a reaction mechanism based on mass balance was proposed, demonstrating that bio-mediated sulfur transformation maintained a sustainable redox reaction with iron (hydr)oxides under fluctuating anaerobic-aerobic-anaerobic conditions tested in this study. Altogether, the finding of our study is critical for obtaining a more complete understanding of the dynamics of iron redox reactions and pollutant transformation in sulfur-rich aquatic environments.

Thermal adaptation of microbial respiration persists throughout long-term soil carbon decomposition.

Soil microbial respiration is expected to show adaptations to changing temperatures, greatly weakening the magnitude of feedback over time, as shown in labile carbon substrates. However, whether such thermal adaptation persists during long-term soil carbon decomposition as carbon substrates decrease in decomposability remains unknown. Here, we conducted a 6-year incubation experiment in natural and arable soils with distinct properties under three temperatures (10, 20 and 30°C). Mass-specific microbial respiration was consistently lower under higher long-term incubation temperatures, suggesting the occurrence and persistence of microbial thermal adaptation in long-term soil carbon decomposition. Furthermore, changes in microbial community composition and function largely explained the persistence of microbial respiratory thermal adaptation. If such thermal adaptation generally occurs in large low-decomposability carbon pools, warming-induced soil carbon losses may be lower than previously predicted and thus may not contribute as much as expected to greenhouse warming.

Assessment of the Health of Soils Contaminated with Ag, Bi, Tl, and Te by the Intensity of Microbiological Activity.

Soil health is the basis of ecological and food security for humanity. Among the informative indicators of soil health are microbiological indicators based on the intensity of the carbon dioxide release from the soil. The reaction of the microbial community of Haplic Chernozem Loamic, Haplic Arenosols Eutric, and Haplic Cambisols Eutric to contamination with oxides and nitrates of Ag, Bi, Tl, and Te at doses of 0.5, 1, 3, 10, and 30 derived specific permissible concentrations (SPC) was analyzed in the conditions of a vegetation experiment (the exposure period was 10 days). One derived concentration is assumed to be equal to three background concentrations of the element in the soil. The carbon content of microbial biomass in Haplic Chernozem varied between the experimental options from 6 to 218 mg/kg of soil; in Haplic Arenosols, from 3 to 349 mg/kg of soil; and in Haplic Cambisols, from 7 to 294 mg/kg of soil. Microbial biomass was a more sensitive indicator of contamination by the studied pollutants than basal soil respiration. A decrease in specific microbial respiration was found when Haplic Cambisols were contaminated with Ag, Bi, Te, and Tl oxides. Te and Tl nitrates had a significant toxic effect on each type of soil. At the maximum dose of Tl and Te nitrate, a decrease in basal soil respiration of 56-96% relative to the control and an increase in the metabolic coefficient by 4-6 times was found. The toxicity series of heavy metals averaged for all types of soils in terms of microbiological activity was established: Bi > Ag > Te > Tl (oxides) and Te > Tl > Ag > Bi (nitrates). Nitrates of the elements were more toxic than oxides. Soil toxicity due to Ag, Bi, Tl, and Te contamination was dependent on soil particle size distribution, organic matter content, and soil structure. A series of soil sensitivity to changes in microbial biomass and basal soil respiration when contaminated with the studied pollutants: Haplic Arenosols > Haplic Chernozems > Haplic Cambisols. When diagnosing and assessing the health of soils contaminated with Ag, Bi, Tl, and Te, it is advisable to use indicators of soil microbiological activity.

Solar radiation explains litter degradation along alpine elevation gradients better than other climatic or edaphic parameters.

Organic matter (OM) decomposition has been shown to vary across ecosystems, suggesting that variation in local ecological conditions influences this process. A better understanding of the ecological factors driving OM decomposition rates will allow to better predict the effect of ecosystem changes on the carbon cycle. While temperature and humidity have been put forward as the main drivers of OM decomposition, the concomitant role of other ecosystem properties, such as soil physicochemical properties, and local microbial communities, remains to be investigated within large-scale ecological gradients. To address this gap, we measured the decomposition of a standardized OM source - green tea and rooibos tea - across 24 sites spread within a full factorial design including elevation and exposition, and across two distinct bioclimatic regions in the Swiss Alps. By analyzing OM decomposition via 19 climatic, edaphic or soil microbial activity-related variables, which strongly varied across sites, we identified solar radiation as the primary source of variation of both green and rooibos teabags decomposition rate. This study thus highlights that while most variables, such as temperature or humidity, as well as soil microbial activity, do impact decomposition process, in combination with the measured pedo-climatic niche, solar radiation, very likely by means of indirect effects, best captures variation in OM degradation. For instance, high solar radiation might favor photodegradation, in turn speeding up the decomposition activity of the local microbial communities. Future work should thus disentangle the synergistic effects of the unique local microbial community and solar radiation on OM decomposition across different habitats.

Cropland abandonment alleviates soil carbon emissions in the North China Plain.

Land use change could profoundly influence the terrestrial ecosystem carbon (C) cycle. However, the effects of agricultural expansion and cropland abandonment on soil microbial respiration remain controversial, and the underlying mechanisms of the land use change effect are lacking. In this study, we conducted a comprehensive survey in four land use types (grassland, cropland, orchard, and old-field grassland) of North China Plain with eight replicates to explore the responses of soil microbial respiration to agricultural expansion and cropland abandonment. We collected surface soil (0-10 cm in depth) in each land use type to measure soil physicochemical property and microbial analysis. Our results showed that soil microbial respiration was significantly increased by 15.10 mg CO2 kg-1 day-1 and 20.06 mg CO2 kg-1 day-1 due to the conversion of grassland to cropland and orchard, respectively. It confirmed that agricultural expansion might exacerbate soil C emissions. On the contrary, the returning of cropland and orchard to old-field grassland significantly decreased soil microbial respiration by 16.51 mg CO2 kg-1 day-1 and 21.47 mg CO2 kg-1 day-1, respectively. Effects of land use change on soil microbial respiration were predominately determined by soil organic and inorganic nitrogen contents, implying that nitrogen fertilizer plays an essential role in soil C loss. These findings highlight that cropland abandonment can effectively mitigate soil CO2 emissions, which should be implemented in agricultural lands with low grain production and high C emissions. Our results improve mechanistic understanding on the response of soil C emission to land use changes.

Electromotive force induced by dynamic magnetic field electrically polarized sediment to aggravate methane emission.

As a primary driving force of global methane production, methanogens like other living organisms are exposed to an environment filled with dynamic electromagnetic waves, which might induce electromotive force (EMF) to potentially influence the metabolism of methanogens. However, no reports have been found on the effects of the induced electromotive force on methane production. In this study, we found that exposure to a dynamic magnetic field enhanced bio-methanogenesis via the induced electromotive force. When exposed to a dynamic magnetic field with 0.20 to 0.40 mT of intensity, the methane emission of the sediments increased by 41.71%. The respiration of methanogens and bacteria was accelerated by the EMF, as the ratios of F420H2/F420 and NAD+/NADH of the sediment increased by 44.12% and 55.56%, respectively. The respiratory enzymes in respiration chains might be polarized with the EMF to accelerate the proton-coupled electron transfer to enhance microbial metabolism. Together with the enriched exoelectrogens and electrotrophic methanogens, as well as the increased sediment electro-activities, this study indicated that the EMF could enhance the electron exchange among extracellular respiratory microorganisms to increase the methane emission from sediments.

Spatially explicit estimate of nitrogen effects on soil respiration across the globe.

Soil respiration (Rs), as the second largest flux of carbon dioxide (CO2 ) between terrestrial ecosystems and the atmosphere, is vulnerable to global nitrogen (N) enrichment. However, the global distribution of the N effects on Rs remains uncertain. Here, we compiled a new database containing 1282 observations of Rs and its heterotrophic component (Rh) in field N manipulative experiments from 317 published papers. Using this up-to-date database, we first performed a formal meta-analysis to explore the responses of Rs and Rh to N addition, and then presented a global spatially explicit quantification of the N effects using a Random Forest model. Our results showed that experimental N addition significantly increased Rs but had a minimal impact on Rh, not supporting the prevailing view that N enrichment inhibits soil microbial respiration. For the major biomes, the magnitude of N input was the main determinant of the spatial variation in Rs response, while the most important predictors for Rh response were biome specific. Based on the key predictors, global mapping visually demonstrated a positive N effect in the regions with higher anthropogenic N inputs (i.e., atmospheric N deposition and agricultural fertilization). Overall, our analysis not only provides novel insight into the N effects on soil CO2 fluxes, but also presents a spatially explicit assessment of the N effects at the global scale, which are pivotal for understanding ecosystem carbon dynamics in future scenarios with more frequent anthropogenic activities.

Soil contamination by diesel fuel destabilizes the soil microbial pools: Insights from permafrost soil incubations.

Arctic contamination by diesel fuel (DF) is of great concern because of the uncertain feedback of permafrost carbon (C) and soil microbiota to DF in the context of climate change in high latitudes. We conducted a laboratory incubation experiment with a gradient of DF addition ratios to examine the responses of the soil microbiota of the typical permafrost soils in the tundra ecosystems of the Norilsk region (Siberia). The study revealed initial heterogeneity in the microbial activity of the studied soils (Histic Gleyic Cryosols (CR-hi,gl), Turbic Cryosols (CR-tu), Turbic Spodic Folic Cryosols (CR-tu,sd,fo), Gleyic Fluvisols (FL-gl)). We applied the two-pool model for evaluation of the effect of DF on the proportions of C pools and revealed significant differences between soil types in the fast and slow C pools in response to DF addition. The results showed that DF addition treatments had varying effects on the fast and slow C pools, microbial activity, and microbial community structure in the studied soils. For minor exceptions, DF dramatically accelerated C loss from the slow C pool in all soil types. We assume that differences in C pool and microbiota responses to DF addition were caused by soil texture and changes in microbial community structure. We isolated Serratia proteamaculans, S. liquefaciens, S. plymuthica, Rhodococcus erythropolis, Pseudomonas antarctica, P. libanensis, P. brassicacearum, and P. chlororaphis from the DF-polluted soils. These species are recommended for bioremediation to mitigate the DF contamination of permafrost soils, especially regarding climate change and the sustainable well-being of Arctic ecosystems.