Physicochemical characterization of antioxidant film based on ternary blend of chitosan and Tulsi-Ajwain essential oil for preserving walnut.

This study focuses on changes in the physiochemical properties of chitosan film when incorporated with a blend of essential oils of Tulsi and Ajwain. The essential oil blend-loaded films showed a decrement in transparency. Tulsi essential oil decreased the moisture content, swelling capacity, and water solubility. However, adding Ajwain along with Tulsi essential oil led to a significant increase in these properties. Meanwhile, the water vapor transmission rate didn't change significantly due to non-polar constituents in Tulsi essential oil, except when only Ajwain essential oil was present. The mechanical properties showed that the tensile strength of films increased with the addition of Tulsi essential oil (14.95 MPa to 31.27 MPa) but decreased further with increasing Ajwain oil concentration in films (32.13 MPa to 15.89 MPa). On the other hand, an increment in percent elongation at break (8.26 % to 24.02 %) was observed due to the excellent plasticization effect of Ajwain essential oil. Antioxidant activity was observed for the Tulsi essential oil-containing films and increased significantly with adding Ajwain essential oil. Finally, walnuts were packed in the active film. The active film showed better antioxidant activity against the oxidation of oil in walnuts, which the FTIR of the packed product confirmed.

Use of aromatic plants and essential oils in the teaching of physics and chemistry to enhance motivation and sustainability awareness among primary education trainee teachers.

This work faces the known problem of negative visión among prospective primary teachers regarding science teaching and learning. This is even more acute if gender is taken into account. To overcome this, the use of ethnobotany as a science of proximity is proposed. Specifically, an educational intervention is described that is based on a combination of active methodologies and the use of ethnobotany as a teaching tool for a subject with physics and chemistry contents. Ninety-two university-based primary education trainee teachers participated in the research. By means of questionnaires, information was collected regarding attitudes toward the subject and the use of ethnobotany as a teaching tool, evaluation of the proposal, sustainability awareness, and affective and emotional dimensions. The data indicated a great impact and acceptability. The results show a significant increase in motivation, an improvement in attitudes toward sustainable behavior and a favorable assessment of the proposed didactic tool. This hybrid methodology demonstrates, in the context used, effectiveness in improving the perception of science.

Integrated supercritical fluid extraction of essential oils.

Supercritical fluid extraction (SFE) stands out as an incredibly efficient, environmentally conscious, and fast method for obtaining essential oils (EOs) from plants. These EOs are abundant in aromatic compounds that play a crucial role in various industries such as food, fragrances, cosmetics, perfumery, pharmaceuticals, and healthcare. While there is a wealth of existing literature on using supercritical fluids for extracting plant essential oils, there's still much to explore in terms of combining different techniques to enhance the SFE process. This comprehensive review presents a sophisticated framework that merges SFE with EO extraction methods. This inclusive categorization encompasses a range of methods, including the integration of pressurized liquid processes, ultrasound assistance, steam distillation integration, microfluidic techniques, enzyme integration, adsorbent facilitation, supercritical antisolvent treatments, molecular distillation, microwave assistance, milling process and mechanical pressing integration. Throughout this in-depth exploration, we not only elucidate these combined techniques but also engage in a thoughtful discussion about the challenges they entail and the array of opportunities they offer within the realm of SFE for EOs. By dissecting these complexities, our objective is to tackle the current challenges associated with enhancing SFE for commercial purposes. This endeavor will not only streamline the production of premium-grade essential oils with improved safety measures but also pave the way for novel applications in various fields.

Essential oils mouthwash with or without alcohol in relation to effect on parameters of plaque and gingivitis: A systematic review and meta-analysis.

AIM: The primary aim was to systematically assess the available literature on the effect of an essential oils mouthwash without alcohol (EOalc-) compared to an essential oils mouthwash with alcohol (EOalc+) on plaque scores and parameters of gingival health. The secondary aim was to evaluate user appreciation. MATERIALS AND METHODS: The MEDLINE-PubMed and Cochrane-CENTRAL databases were searched to identify eligible studies published up to and including March 2024. Papers comparing the effectiveness of EOalc- and EOalc+ were included. The quality was assessed. A descriptive analysis and a meta-analysis were performed. RESULTS: After screening, seven papers were found to be eligible. The descriptive analysis demonstrates a significant difference in plaque scores in favour of EOalc+. This is confirmed by the meta-analyses of plaque scores in non-brushing and brushing studies (DiffM = 0.40; 95% CI [0.27; 0.53], p < 0.00001 and DiffM = 0.05; 95% CI [0.01; 0.10], p = 0.01, respectively). This finding is also supported by the sub-analysis of brands. The meta-analyses of bleeding and gingival scores in brushing studies did not show significant differences between products. For user appreciation, the difference found was for taste perception in favour of EOalc- (DiffM = 1.63; 95% CI [0.72; 2.55], p = 0.0004). CONCLUSION: When an EO-mouthwash is used in non-brushing or brushing situations, with small to moderate certainty, EOalc- provided less effect regarding plaque control than EOalc+. For bleeding and gingival index there is weak certainty for no difference. In terms of the taste perception EOalc- seems more appreciated.

Phytochemical Characterization, Fumigant And Contact Toxicity Activities Of Four Essential Oils Against Eriophyid Gall Mite, Aceria pongamiae Keifer (Acarina: Eriophyidae).

Plant-derived essential oils (EO) offer natural alternative to chemical pesticides for eco-friendly pest control approaches. Aceria pongamiae Keifer, a notorious pest that affects Pongamia pinnata (L.) Pierre has mainly been controlled using synthetic acaricides leading to resistance development and environmental issues. EOs provide natural biodegradable option for pest control with unique mode of action. Study evaluates acaricidal efficacy of EOs-eucalyptus Eucalyptus maculata Hook (EEO), lavender Lavandula angustifola L. (LEO), peppermint Mentha piperita L. (PEO) and black pepper Piper nigrum L. (BPEO) against A. pongamiae for the first time. Biological activity of EOs using fumigation and contact toxicity assays at concentrations ranging from 0.1 to 1% and 0.6 to 0.9% respectively, overexposure periods of 24, 48 and 72h was investigated. Chemical characterization was performed using GC-MS analysis. Eucalyptol (62.88%), linalyl acetate (39.11%), menthol (44.35%) and caryophyllene (32.77%) were the main components of EEO, LEO, PEO and BPEO respectively. After 24h observation, EEO (LC50=1.01%) and after 48 and 72h, PEO had the highest fumigant toxicity (LC50=0.71 and 0.29% respectively). The BPEO showed the most contact toxicity after 24, 48 and 72h (LC50=0.92, 0.68 and 0.46% respectively). This work reinforces the selection of adequate essential oils for implementation in future pest control strategies.

Eugenia pohliana leaves essential oil inhibits fungal virulence and restores antibiotic efficacy in multidrug-resistant bacterial strains.

This study aims to investigate the essential oil from leaves of E. pohliana (EOEP) in regard to its chemical composition, antimicrobial and drug-enhancing activity, as well as the reduction of fungal virulence capacity. Chemical characterization using GC-MS showed as major components the sesquiterpenes δ-cadinene, Epi-α-Muurolol, and bicyclogermacrene. The results of antibacterial tests indicated that Staphylococcus aureus was more sensitive to EOEP, that also enhanced the efficacy of gentamicin, erythromycin, and norfloxacin. EOEP exhibited antifungal properties against Candida albicans, in addition to potentiating the effectiveness of fluconazole against Candida tropicalis. It showed anti-virulence effects in all fungal strains. These findings underscore Eugenia pohliana as a potential candidate for the prospection of novel therapeutic agents to treat infectious diseases caused by resistant microbes.

Antimicrobial activities of essential oils of plants species from Morocco against some microbial strains.

BACKGROUND: Essential oils have important antibacterial activities and can successfully replace antibiotics, which show their inefficiency, especially against fungi and multi-resistant bacteria. OBJECTIVE: The main purpose of our research was to investigate the antibacterial and antifungal activity of essential oils from fifteen plants harvested in the Taroudant region. MATERIAL AND METHODS: In this work, the essential oils were extracted by hydrodistillation using a Clevenger-type apparatus. The method of disc diffusion in agar medium (aromatogram) is the one used to evaluate the activity of these essential oils against four pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Bacillus sp., and Enterococcus cloacae) and two yeasts (Candida albicans and Cryptococcus neoformans). RESULTS: Our findings, show that all of the plants' leaves yielded extremely aromatic essential oils that differed in look and color. Furthermore, the 93.33% of the fifteen essential oils that were evaluated proved to be effective against at least one kind of bacteria or fungus. This suggests that the proportion of essential oils with no antibacterial action was rather low, at around 7%. Our data also showed that the freshness or dryness of the plant at the time of harvest could affect the extraction rate of essential oils. This screening showed us that these essential oils present inhibitory activities towards the studied Gram+ bacteria, as well as a resistance against Gram-, in particular Enterococcus cloacae. CONCLUSION: These essential oils can therefore be used in the prevention and treatment of certain infectious diseases and to fight against bacteria that are multi-resistant to the usual antibiotics.

Antimicrobial, anti-enzymatic and antioxidant activities of essential oils from some Tunisian Eucalyptus species.

Many plants can produce essential oils (EOs), having various biological properties. This study evaluated the antioxidant, anti-enzymatic and antimicrobial effects of the EOs derived from leaves of Eucalyptus cladocalyx, E. angulosa, E. microcorys, E. ovata, E. diversicolor, E. saligna, E. sargentii and E. resinifera. The antioxidant activity of the EOs was carried out with three different methods (ABTS, DPPH and FRAP). In addition, their anti-colinesterases, anti α-amylase and anti α-glucosidase effects were assessed by spectrophotometric assays. The antimicrobial activities were tested against six phytopathogenic bacterial strains, including two G + ve (Bacillus mojavensis and Clavibacter michiganensis) and four G-ve (Pseudomonas fluorescence, P. syringae, Xanthomonas campestris and E. coli). The current study has also investigated the inhibition of biofilm formation and the possible effect on bacterial cells biofilm metabolism of three Gram-negative (Pseudomonas aeruginosa, Escherichia coli and Acinetobacter baumannii) and two Gram-positive pathogenic bacteria (Staphylococcus aureus and Listeria monocytogenes). The ABTS and DPPH tests indicated that E. diversicolor and E. saligna EOs showed high antioxidant activities, whereas FRAP test suggested that E. diversicolor EO exhibited the better antioxidant activity. E. resinifera and E. ovata EOs were the most active against cholinesterases instead E. ovata and E. sargentii EOs were more active against enzymes involved in diabetes. Antibacterial assays revealed that E. ovata and E. saligna EOs possess significant activity closely to tetracycline. Whereas, the antifungal assay revealed that all EOs have effectively suppressed the tested fungal growth. E. saligna EO showed substantial efficacy inhibiting both the mature biofilm (85.40 %) and metabolic activities (89.80 %) of L. monocytogenes. These results demonstrate the wide range of possible uses for Eucalyptus EOs in both agriculture and medicine fields, suggesting potential uses as strong antibiofilm agents and for biocontrol of phytopathogens.

Recent advances in essential oils and their nanoformulations for poultry feed.

Antibiotics in poultry feed to boost growth performance are becoming increasingly contentious due to concerns over antimicrobial resistance development. Essential oils (EOs), as natural, plant-derived compounds, have demonstrated antimicrobial and antioxidant properties. EOs may potentially improve poultry health and growth performance when included in poultry feed. Nevertheless, the incorporation of EOs as nutritional additives is hindered by their high volatility, low water solubility, poor intestinal absorption, and sensitivity to environmental conditions. Recently, nanoencapsulation strategies using nanoformulations have emerged as a potential solution to these challenges, improving the stability and bioavailability of EOs, and enabling targeted delivery in poultry feed. This review provides an overview of the antioxidant and antibacterial properties of EOs, the current limitations of their applications in poultry feed, and the recent advancements in nano-engineering to overcome these limitations. Furthermore, we outline the potential future research direction on EO nanoformulations, emphasizing their promising role in advancing sustainable poultry nutrition.Highlights• Essential oils (EOs) are known as powerful antioxidants and antibacterial agents.• EOs have a high potential to replace antibiotics as feed additives.• Nanoformulations of EOs have shown improved bioactivity and storage stability of EOs.• Nanoformulation promotes the bioavailability and gut adsorption of EOs as feed additives.

Novel Collagen-Based Emulsions Embedded with Palmarosa Essential Oil, and Chamomile and Calendula Tinctures, for Skin-Friendly Textile Materials.

Skin-friendly textile materials were obtained by applying oil-in-water emulsions based on palmarosa essential oil, chamomile, and calendula tinctures onto cotton fabrics. Different formulations based on these bioactive principles incorporated in collagen as polymeric matrices were prepared and immobilized on a plain weave textile structure from 100% cotton. The functionalized textile materials were characterized in terms of physicochemical, mechanical, antibacterial, and biocompatibility points of view. The pH values of the prepared emulsions were in the range of 4.81-5.23 and showed no significant differences after 4 h of storage. Moreover, the addition of a higher quantity of active principles (palmarosa essential oil and plant tinctures) caused slightly lower values of acidic pH. The electrical conductivity of the obtained emulsions increased with the decrease in the oil phases in the system. The highest values were obtained for the emulsion developed with the smallest volume fraction of active principle-palmarosa essential oil and plant tinctures. The emulsion that contained the least amount of collagen and the highest number of active principles exhibited the lowest stability. The textile materials treated with synthesized emulsions exerted antibacterial effects against S. aureus and E. coli strains and did not affect keratinocyte growth, spreading, and organization, highlighting the biocompatibility of these developed skin-friendly textiles.

Turmeric-Black Cumin Essential Oils and Their Capacity to Attenuate Free Radicals, Protein Denaturation, and Cancer Proliferation.

Turmeric rhizomes (Curcuma longa) and black cumin seeds (Nigella sativa) are polyherbal ingredients used for the management of cancer and other chronic inflammatory diseases in Nigerian ethnomedicine. Previous studies have shown the antioxidant, anti-inflammatory, and anticancer activities of the individual plant extracts. However, the two spices have not been biologically potentiated in their combined form. Therefore, this study obtained essential oils (EOs) from the combined spices and evaluated their inhibitory effects on free radicals, protein denaturation, and cancer proliferation. The EOs were extracted by hydro-distillation (HD) and characterized by gas chromatography-mass spectrometry (GC-MS). In vitro antioxidant assessment was conducted based on DPPH, hydrogen peroxide (H2O2), nitric oxide (NO), and ferric ion (Fe3+) radical scavenging assays. The cytotoxicity of the oil against non-tumorigenic (HEK293) and cancerous (HepG2 and HeLa) cell lines was determined following the MTT cell viability assay. An in silico molecular docking analysis of the oil constituents was also performed. Six batches of EOs I-VI were afforded, comprising twenty-two major constituents, with aromatic Ar-turmerone being the most prominent compound. There was a marked improvement in the bioactivity of the oils upon repeated HD and as a combination. The batch VI oil exhibited the best activity, with a cytotoxicity (CC50) of 10.16 ± 1.69 µg/100 µL against the HepG2 cell line, which was comparable to 5-fluorouracil (standard, CC50 = 8.59 ± 1.33 µg/100 µL). In silico molecular docking suggested δ-curcumene, Ar-curcumene, Ar-turmerol, and Ar-turmerone among the promising compounds based on their high binding energy scores with NOX2, NF-κB, and mdm2 proteins. In conclusion, the oils from the turmeric-black cumin combined possess a considerable inhibition ability against free radicals, protein denaturation, and cancer proliferation. This study's findings further underscore the effectiveness of turmeric-black cumin as a polyherbal medicinal ingredient.

Evaluation of the effectiveness of some essential oils against zoonotic methicillin-resistant Staphylococcus aureus isolated from dairy products and humans.

Objective: Staphylococcus aureus (S. aureus) is a zooanthroponotic, nosocomial, and community-associated pathogen that threatens livestock management and even public health. The goal of this investigation was to clarify the role of S. aureus in zoonotic illnesses. Besides that, a novel trial was conducted in the current Egyptian study using oil extracts such as cactus oil, tea oil, geranium oil, and thyme oil to demonstrate the susceptibility of methicillin-resistant S. aureus (MRSA) isolates to these organic oils in response to the alarming global concern regarding the decreased susceptibility of S. aureus to known antibiotics, which exacerbates control and treatment protocols. Materials and Methods: A total of 110 samples (45 raw cattle milk samples, 35 Karish cheese samples, and 30 human sputum samples) were collected. The bacterium was identified via traditional culturing methods, Gram staining, and the application of several biochemical tests. After that, various kinds of known commercial antibiotics were used to detect the antimicrobial susceptibility (AMS) of the obtained isolates. Furthermore, conventional polymerase chain reaction (PCR) testing was performed to identify S. aureus (nuc gene) and MRSA (mecA gene), with further application of multiplex PCR for screening of all the obtained isolates for vancomycin resistance via targeting vanA, vanB, and vanC genes. Finally, the agar gel diffusion method was performed to assess the antibacterial activity of four plant extracts (cactus oil, tea oil, geranium oil, and thyme oil) against the obtained MRSA. Results: The culturing method revealed S. aureus positivity in raw cattle milk (13.33%), in Karish cheese (28.57%), and in human samples (20%). The obtained isolates showed mainly resistance to amoxicillin-clavulanic and ampicillin antibiotics, while the dairy samples showed further resistance against ceptaxime and an intermediate reaction against erythromycin. On the molecular side, PCR positivity was present in human samples (10%), raw cow milk (13.33%), and Karish cheese (14.29%). Nine of the fourteen PCR isolates were methicillin-resistant S. aureus (MRSA) isolates. Comparing the four oil extracts against the acquired MRSA isolates, cactus oil extract proved to be the most effective. Conclusion: The study's results are highly promising as they support the notion that certain essential oils possess strong antimicrobial properties against zoonotic S. aureus, thereby reducing the excessive use of antibiotics in veterinary and medical settings.

Monoterpenoid synergy: a new frontier in biological applications.

Monoterpenoids, compounds found in various organisms, have diverse applications in various industries. Their effectiveness is influenced by the oil's chemical composition, which in turn is influenced by plant genotype, environmental conditions, cultivation practices, and plant development stage. They are used in various industries due to their distinctive odor and taste, serving as ingredients, additives, insecticides, and repellents. These compounds have synergistic properties, resulting in superior combined effects over discrete ones, potentially beneficial for various health purposes. Many experimental studies have investigated their interactions with other ingredients and their antibacterial, insecticidal, antifungal, anticancer, anti-inflammatory, and antioxidant properties. This review discusses potential synergistic interactions between monoterpenoids and other compounds, their sources, and biological functions. It also emphasizes the urgent need for more research on their bioavailability and toxicity, underlining the importance and relevance of this comprehensive study in the current scientific landscape.

THE COMBINATORY EFFECTS OF ESSENTIAL OIL FROM Lippia macrophylla ON MULTIDRUG RESISTANT Acinetobacter baumannii CLINICAL ISOLATES.

To assess the antibacterial effectiveness of Lippia macrophylla essential oil (LMEO) against multidrug-resistant Acinetobacter baumannii isolates, both as a standalone treatment and in combination with conventional antibiotics. LMEO demonstrated a significant inhibitory effect on the growth of A. baumannii, with a minimum inhibitory concentration (MIC) below 500µg/mL. Notably, LMEO was capable of reversing the antibiotic resistance of clinical isolates or reducing their MIC values when used in combination with antibiotics, showing synergistic (FICI ≤ 0.5) or additive effects. The combination of LMEO and imipenem was particularly effective, displaying synergistic interactions for most isolates. Ultrastructural analyses supported these findings, revealing that the combination of LMEO + ceftazidime compromised the membrane integrity of the Acb35 isolate, leading to cytoplasmic leakage and increased formation of Outer Membrane Vesicles (OMVs). Taken together our results point for the use of LMEO alone or in combination as an antibacterial agent against A. baumannii. These findings offer promising avenues for utilizing LMEO as a novel antibacterial strategy against drug-resistant infections in healthcare settings, underscoring the potential of essential oils in enhancing antibiotic efficacy.

Optimization of Enzyme-Assisted Extraction of Rosemary Essential Oil Using Response Surface Methodology and Its Antioxidant Activity by Activating Nrf2 Signaling Pathway.

Rosemary essential oil (REO) is widely recognized as a food flavoring and traditional herb and possesses potential antioxidant activity. However, its low yield rate and unclarified antioxidant mechanism warrant further investigation. In this study, an enzyme pretreatment-assisted extraction method with Box-Behnken design (BBD) and response surface methodology (RSM) models was employed to optimize the main factors of REO, and its antioxidant molecular mechanism under oxidative stress was elucidated in hydrogen peroxide-induced human lung carcinoma (A549) cells. The optimized yield (4.10%) of REO was recorded with the following optimum conditions: enzyme amount 1.60%, enzyme digestion pH 5.0, enzyme digestion temperature 46.50 °C, and enzyme digestion time 1.7 h. Meanwhile, 1.8-cineole (53.48%) and ß-pinene (20.23%) exhibited radical scavenging activity higher than that of BHA and BHT. At the cellular level, REO (12.5-50 µg/mL) increased the levels of cell viability, CAT, SOD, and GSH significantly while reducing the contents of ROS, MDA, and GSSG, when compared to H2O2 exposure. Mechanically, REO relieved oxidative stress via activating the Nrf2 signaling pathway and enhancing the protein expression of Nrf2, NQO-1, and HO-1, which was further verified by molecular docking between the main component 1.8-cineole and the Kelch domain of KEAP1. Therefore, REO could be considered as a potent natural antioxidant with a potential strategy in the food and pharmaceutical industries.

Imiquimod-Loaded Nanosystem for Treatment Human Papillomavirus-Induced Lesions.

Human papillomavirus (HPV)-associated cervical cancer is the most common cancer among women worldwide. The treatment options are strongly related to increased infertility in women. Imiquimod (IQ) is an imidazoquinoline, which has proven antiviral effects against persistent HPV infection by activating immune cells via Toll-like receptors 7/8 when formulated in carriers, like nanogels, for topical use. An effective alternative to conventional therapies is the nanoparticle drug delivery system. We studied lipidic nanoparticles with IQ (Lipo IQ) and functionalized them with a DNA aptamer, AT11 (Lipo IQ AT11), to improve the selectivity for cervical cancer cells combined with the efficacy of essential oils. The formulations showed that the physicochemical properties are adequate for vaginal drug delivery and have antimicrobial activity at higher concentrations (with MIC50 starting from 0.625%). The final formulations exhibited cytotoxicity in cancer cells, enhanced by essential oils without affecting healthy cells, resulting in less than 10% cell viability in HeLa cells and over 60% in NHDF cells. Essential oils potentiate Lipo IQ's effectiveness, while AT11 increases the selectivity for cervical cancer cells. As suggested by the results of the permeation assay, the formulations were internalized by the cancer cells. Overall, the obtained results suggested that the synergistic effect of the essential oils and the nanosystem potentiate the cytotoxic effect of Lipo IQ and that Lipo IQ AT11 promotes selectivity towards cancer cells.

Synergistic Effect of Essential Oils and Antifungal Agents in Fighting Resistant Clinical Isolates of Candida auris.

Recently, a large number of nosocomial infections have been caused by an emerging pathogen that is rising as a worldwide issue in human health: Candida auris. This yeast is considered resistant to antifungals of the first-line therapies, and consequently it is related to morbidity and mortality. Therefore, the aim of this research was to determine the in vitro anti-C. auris activity against twenty-three resistant clinical strains of different essential oils (EOs), pure or in combination with traditional antifungal agents, mainly caspofungin, fluconazole, micafungin and 5-flucytosine. Broth dilution assay was performed to evaluate the fungistatic and fungicidal effectiveness of fifteen EOs towards all the C. auris isolates. The data demonstrated that EOs were able to prevent C. auris growth, with MIC values ranging from 0.03 to 1% for the efficacious EOs (thyme, cinnamon, geranium, clove bud, lemongrass and mentha of Pancalieri), whereas the MICs were >1% for the ineffective ones. Thereafter, the six most effective EOs were used to perform the checkerboard experiments by assaying simultaneously the activity of EOs and traditional antifungals towards two selected strains. The most promising synergic combinations towards C. auris, depending on the isolate, were those with micafungin and geranium, thyme, cinnamon, lemongrass or clove bud EOs, with fluconazole and mentha of Pancalieri EO, and with 5-flucytosine and mentha of Pancalieri EO. These EOs and their combinations with antifungal drugs may provide a useful therapeutic alternative that could reduce the dose of the individual components, limiting the overall side effects. These associations might be a prospective option for the future treatment of infections, thus helping to overcome the challenging issue of resistance in C. auris.

Salvia verticillata (L.)-Biological Activity, Chemical Profile, and Future Perspectives.

Species belonging to the genus Salvia, Lamiaceae, have been deeply involved in the folk medicine of different nations since ancient times. Lilac sage, or Salvia verticillata L. (S. verticillata) is a less studied species from the genus. However, it seems to have a prominent potential for the future drug discovery strategies of novel phytopharmaceuticals. This review aims to summarise the data on the biological activity and the phytochemical profile of extracts and essential oils derived from S. verticillata. This review is based on data from 57 in vitro and in vivo studies. The chemical profile of S. verticillata includes different synergic compounds like phenolic acids, flavonoids, terpenes, and salvianolic acids. Although some small amounts of salvianolic acid B were found in S. verticillata extracts, the major compound among the salvianolic acids is salvianolic acid C, a compound associated with the potential for improving liver fibrosis, cardio- and hepatoprotection, and the inhibition of SARS-CoV-2 infection. The cannabinoid type 2 receptor agonist ß-caryophyllene is one of the major compounds in S. verticillata essential oils. It is a compound with a prominent potential in regenerative medicine, neurology, immunology, and other medical fields. The in vivo and the in vitro studies, regarding S. verticillata highlighted good antioxidant potential, anti-inflammatory, antibacterial, and antifungal activity. S.verticillata was also reported as a potential source of drug candidates for the treatment of neurodegenerative diseases such as Alzheimer's disease, because of the inhibitory activity on the acetylcholinesterase. However, the number of studies in this direction is limited.

Dose-dependent responses: a preliminary investigation into the olfactory effects of essential oil concentrations on canine behavior.

The positive impact of essential oils (EOs) on stress release has been demonstrated in both humans and dogs. Among the EOs known for their anxiety-reducing properties, including Cananga odorata, Citrus aurantium, Cupressus sempervirens, Lavandula angustifolia, and Litsea citrata, there is a lack of consensus on the optimal concentration for efficacy. This exploratory study sought to investigate the effects of olfactory enrichment with a blend of these EOs on dogs introduced to an unfamiliar environment. The authors sought to determine the minimum concentration required to achieve increased relaxation. In a randomized controlled crossover study design, 54 dogs were exposed to 0, 1, 5, and 10 drops of the EO blend applied to their collars before entering an unfamiliar room with their owners. Behavioral observations were employed to quantify the total duration of activity and relaxation related behaviours for each dog under each treatment condition. A significant difference in panting was identified among the treatments (χ2(3) = 9.88; p = 0.020). Dunn-Bonferroni post-hoc tests revealed a significant reduction in panting during the 10 drops treatment compared to the control treatment (p = 0.047). No significant differences were observed for other behaviors. To provide a comprehensive overview of behavioral tendencies in this canine population, owners also completed the Canine Behavioral Assessment and Research Questionnaire (C-BARQ), revealing low scores for anxiety in the study group. These preliminary findings suggest that a concentration of 10 drops of the EO blend on a dog's collar induces increased relaxation, specifically reflected in decreased panting behavior. Lower concentrations did not exhibit a significant relationship with the observed behaviors. These initial findings underscore the importance of exposing dogs to an appropriate concentration of EOs when exploring their potential benefits on welfare among dogs with low anxiety levels. Further research in this area is crucial for a comprehensive understanding of the potential benefits of EOs for canine welfare.

Phytochemical Analysis and Antioxidant and Antifungal Activities of Powders, Methanol Extracts, and Essential Oils from Rosmarinus officinalis L. and Thymus ciliatus Desf. Benth.

Chemical residues in food pose health risks such as cancer and liver issues. This has driven the search for safer natural alternatives to synthetic fungicides and preservatives. The aim of this study was to characterize the chemical composition of the essential oils (EO), determine the polyphenolic contents, and evaluate the in vitro antioxidant and antifungal activities of methanol extracts (ME), essential oils (EO), and powders from Rosmarinus officinalis L. (rosemary) and Thymus ciliatus (Desf) Benth. (thyme) from the M'sila region, Algeria. The chemical composition of the EOs was determined by GC-MS. R. officinalis EO was composed of 31 components, mainly camphor (41.22%), camphene (18.14%), and α-pinene (17.49%); T. ciliatus EO was composed of 58 components, mainly, in percentage, α-pinene (22.18), myrcene (13.13), ß-pinene (7.73), ß-caryophyllene (10.21), and germacrene D (9.90). The total phenols and flavonoids were determined spectrophotometrically, and the rosemary ME was found to possess the highest polyphenolic content (127.1 ± 2.40 µg GAE/mg), while the thyme ME had the highest flavonoid content (48.01 ± 0.99 µg QE/mg). The antioxidant activity was assessed using three methods: rosemary ME was the most potent, followed by DPPH (IC50 = 13.43 ± 0.14 µg/mL), ß-carotene/linoleic acid (IC50 = 39.01 ± 2.16 µg/mL), and reducing power (EC50 = 15.03 ± 1.43 µg/mL). Antifungal activity was assessed for 32 pathogenic and foodborne fungi. Four methods were applied to the solid medium. Incorporating the powdered plant into the culture medium (at 10%) reduced the fungal growth to greater than 50% in 21.88% and 6.25% of all fungal isolates, for R. officinalis and T. ciliatus, respectively. The ME, applied by the well diffusion method (0.1 g/mL), was less effective. Different concentrations of EO were tested. Incorporating the EO into the culture medium (1500 µL/L) inhibited 50% of the molds to levels of 50 and 75% for R. officinalis and T. ciliatus, respectively, with the complete inhibition of four fungi. Fumigated EO (15 µL) inhibited 65% of the molds to levels of 65 and 81.25% for R. officinalis and T. ciliatus, respectively, with the complete inhibition of five fungi. There was little to no sporulation in conjunction with the inhibition. Our results revealed some of the potential of the studied plants to fight foodborne molds and presented their promising characteristics as a source of alternatives to chemical pesticides and synthetic preservatives. Further studies are needed to find adequate application techniques in the food safety area.