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Polysaccharides are biologically essential macromolecules, widely exist in plants, which are used in food, medicine, bioactives' encapsulation, targeted delivery and other fields. Suitable extraction technology can not only improve the yield, but also regulate the physicochemical, improve the functional property, and is the basis for the research and application of polysaccharide. High pressure (HP) extraction (HPE) induces the breakage of raw material cells and tissues through rapid changes in pressure, increases extraction yield, reduces extraction time, and modifies structure of polysaccharides. However, thus far, literature review on the mechanism of extraction, improved yield and modified structure of HPE polysaccharide is lacking. Therefore, the present work reviews the mechanism of HPE polysaccharide, increasing extraction yield, regulating physicochemical and functional properties, modifying structure and improving activity. This review contributes to a full understanding of the HPE or development of polysaccharide production and modification methods and promotes the application of HP technology in polysaccharide production.
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Medicina , Polissacarídeos , Polissacarídeos/farmacologia , Polissacarídeos/química , Extratos Vegetais/química , Substâncias Macromoleculares , Antioxidantes/químicaRESUMO
This investigation aimed to assess the chemical composition and biological activities of bog bilberry (Vaccinium uliginosum L.) leaves. Hydroethanolic extracts were obtained using four extraction techniques: one conventional (CE) and three alternative methods; ultrasound (UAE), microwave (MAE) and high-pressure (HPE) extractions. Spectrophotometric analysis was conducted to determine their chemical content, including the total phenolic content (TPC) and total flavonoid content (TFC). Furthermore, their antioxidative and antimicrobial properties were evaluated. HPLC (high performance liquid chromatography) analysis identified and quantified 17 phenolic compounds, with chlorogenic acid being the predominant compound, with the lowest level (37.36 ± 0.06 mg/g) for the bog bilberry leaf extract obtained by CE and the highest levels (e.g., HPE = 44.47 ± 0.08 mg/g) for the bog bilberry leaf extracts obtained by the alternative methods. Extracts obtained by HPE, UAE and MAE presented TPC values (135.75 ± 2.86 mg GAE/g; 130.52 ± 1.99 mg GAE/g; 119.23 ± 1.79 mg GAE/g) higher than those obtained by the CE method (113.07 ± 0.98 mg GAE/g). Regarding the TFC values, similar to TPC, the highest levels were registered in the extracts obtained by alternative methods (HPE = 43.16 ± 0.12 mg QE/g; MAE = 39.79 ± 0.41 mg QE/g and UAE = 33.89 ± 0.35 mg QE/g), while the CE extract registered the lowest level, 31.47 ± 0.28 mg QE/g. In the case of DPPH (1,1-diphenyl-2-picrylhydrazyl) antioxidant activity, the extracts from HPE, UAE and MAE exhibited the strongest radical scavenging capacities of 71.14%, 63.13% and 60.84%, respectively, whereas the CE extract registered only 55.37%. According to Microbiology Reader LogPhase 600 (BioTek), a common MIC value of 8.88 mg/mL was registered for all types of extracts against Staphylococcus aureus (Gram-positive bacteria) and Salmonella enterica (Gram-negative bacteria). Moreover, the alternative extraction methods (UAE, HPE) effectively inhibited the growth of Candida parapsilosis, in comparison to the lack of inhibition from the CE method. This study provides valuable insights into bog bilberry leaf extracts, reporting a comprehensive evaluation of their chemical composition and associated biological activities, with alternative extraction methods presenting greater potential for the recovery of phenolic compounds with increased biological activities than the conventional method.
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The increasing demand for energy has prompted scholars to research alternative energy sources. Bamboo is a species of woody perennial grass that belongs to the Gramineae family and the Bambuseae subfamily. It could be considered a possible lignocellulosic substrate for the production of bioethanol due to its favourable environmental effects and increased yearly biomass yield. Non-renewable fossil fuels cannot provide enough energy to meet the needs of contemporary societies. Among the various alternative energy sources, bioethanol has drawn a lot of attention from people all around the world. This paper reviews the cost and process parameters for the synthesis of bioethanol from bamboo. This review aims to increase the effectiveness of the entire ethanol production process by focusing on pretreatment, enzymatic hydrolysis, and fermentation. The emphasis of this review is on the efficient process for producing bioethanol while maintaining environmental sustainability. When compared to other NaOH pretreatment techniques, bamboo substrates prepared with NaOH and ultra-high-pressure explosion (UHPE) exhibit higher enzymatic hydrolyzability when processed under optimal conditions, such as 100 MPa, 121 °C, and 70 rpm for 2 h, yielding 89.7-95.1% ethanol after 24 h. The article lists the bamboo species responsible for creating each product, making it straightforward for producers to study and select the species based on whatever value-added product they wish to produce bioethanol with different parameters.
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Biotecnologia , Lignina , Humanos , Biotecnologia/métodos , Hidróxido de Sódio , Lignina/metabolismo , Fermentação , Poaceae/metabolismo , Etanol , Biomassa , Hidrólise , BiocombustíveisRESUMO
As an ingredient in various foods, Chrysanthemum morifolium flower is popular due to its multiple health benefits. Pyrrolizidine alkaloids (PAs) are hepatotoxic secondary metabolites in Chrysanthemum family. Effects of high-pressure extraction (HPE) on PAs removal efficiency, as well as the retention efficiency of functional components, including chlorogenic acid, luteolin-7-ß-D-glucopyranoside, 3,5-dicaffeyl quinic acid and total flavonoids, were investigated and optimized using response surface methodology (RSM). Pressure (0.1-200 MPa), numbers of cycles (1-5) and acetic acid concentration (0-10%) were chosen as the independent variables. The results indicated that the pressure was the most significant factors affecting all responses. The optimum HPE for removing Pas and retaining functional components were set at 124 MPa, with one cycle and with an acetic acid concentration of 10%. After comparing the experimental optimum values and predicted optimum values, the validity of RSM model was proved.
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Jujube contains abundant cyclic adenosine monophosphate (cAMP). In contrast, the extraction technology of cAMP from jujube is still to be explored. In this study, the ultra-high pressure extraction (UHPE) conditions for obtaining the maximum cAMP yield from jujube were optimized. Orthogonal array design (OAD) was applied to evaluate the effects of three variables (pressure, pressure-holding time, and liquid-to-solid ratio) by UHPE on cAMP yield. The results showed that the optimal cAMP yield (1223.2 µg/g) was derived at 300 MPa, 20 min duration, and a liquid-to-solid ratio of 2.5 ml/g. In addition, as an important functional ingredient in jujube, cAMP has potential anti-allergic effect. To develop the functional characteristics of jujube, the effect of cAMP was characterized in vivo with the Balb/c mouse model of peanut allergy, which was established by subcutaneous injection of crude peanut protein extract (PN). The results showed that treatment with cAMP in PN-sensitized mice suppressed the lesions in jejunal tissues and allergic symptoms and restored spleen index. Meanwhile, cAMP treatment reduced serum levels of specific immunoglobulin E (IgE), histamine, as well as interleukin-4 (IL-4) and stimulated the secretion of tumor necrosis factor-α (TNF-α), whereas the serum levels of interleukin-10 (IL-10) were not affected. Our results suggested that cAMP has an anti-allergic effect in PN-sensitized mice.
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Phenolic compounds are important bioactive compounds identified in prickly pear peel that have important antioxidant and antimicrobial properties. However, conventional thermal extraction methods may reduce their bioactivity, and technologies such as high pressure (HP) and ohmic heating (OH) may help preserve them. In this study, both technologies were analyzed, individually and combined (250/500 MPa; 40/70 °C; ethanol concentration 30/70%), and compared with Soxhlet with regard to total phenolics, flavonoids, and carotenoids as well as antioxidant (ABTS, DPPH, ORAC), DNA pro-oxidant, and antimicrobial (inhibition halos, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), growth curves, and viable cells) activities of prickly pear peel extracts. Total phenolics extracted by each technology increased 103% (OH) and 98% (HP) with regard to Soxhlet, but the contents of total flavonoids and carotenoids were similar. Antioxidant activity increased with HP and OH (between 35% and 63%), and OH (70 °C) did not induce DNA degradation. The phenolic compound present in higher amounts was piscidic acid, followed by eucomic acid and citrate. In general, their extraction was significantly favored by HP and OH. Antimicrobial activity against 7 types of bacteria showed effective results only against S. aureus, S. enteritidis, and B. cereus. No synergetic or additive effect was observed for HP/OH.
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BACKGROUND: The consumption of vegetable juices has increased due to their characteristics such as freshness/naturalness, high nutritional value, low in calories, and for being a convenient way of consuming bioactive compounds. High hydrostatic pressure (HPP), which has been mainly used to replace thermal processing, is now also being successfully applied as extraction technology to recover bioactive compounds from herbs. The present work aimed to evaluate the effect of supplementation of carrot juice with winter savory leaf aqueous extract on the final juice characteristics. RESULTS: The extract was added to raw carrot juice (1.0 mg mL-1 ), which was then submitted to HPP and stored for 15 days under refrigeration. Microbial analyses were performed during storage time, as also were analyzed the physicochemical properties such as pH, colour, bioactive compound concentration and antioxidant activity. Supplemented juices presented lower microbial counts than the non-supplemented ones, and, generally, did not present significant changes (P > 0.05) in pH or colour. Concerning the total phenolics and total flavonoids, as well as antioxidant activity, the values were generally higher (P < 0.05) in supplemented juices, which was proven by the high correlation found between total phenolics and ABTSâ+ and FRAP assays. CONCLUSIONS: These data suggest that the addition of winter savory leaf extract in carrot juice treated with HPP can effectively improve microbial safety throughout refrigerated storage as well as antioxidant activity, without risking other characteristics of the juice, such as the colour or the acidity. © 2020 Society of Chemical Industry.
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Daucus carota/química , Aditivos Alimentares/análise , Conservação de Alimentos/métodos , Sucos de Frutas e Vegetais/análise , Extratos Vegetais/análise , Satureja/química , Antioxidantes/análise , Manipulação de Alimentos/instrumentação , Manipulação de Alimentos/métodos , Concentração de Íons de Hidrogênio , Pressão Hidrostática , Valor Nutritivo , Folhas de Planta/químicaRESUMO
Satureja montana L. has several biological properties related to its diverse composition of secondary metabolites. Nevertheless, it has been mainly studied for its essential oil, with only a few studies on the profile and bioactivities of the bioactive compounds from its leaf extracts being reported. This work aimed to study the antioxidant activity (by oxygen radical absorbance capacity (ORAC) assay), antimicrobial minimum inhibitory and bactericidal concentrations (MIC and MBC) determination, antibiofilm (by colorimetry), impact upon DNA (anti- and pro-oxidant assay), and cytotoxicity (by cell metabolism viability assays) of S. montana extracts obtained by high-pressure-assisted extraction (HPE). The extract obtained at 348 MPa, 35% (v/v) ethanol presented the highest concentration of individual phenolic compounds, and a minimum bactericidal concentration of 20 mg/mL against Listeria monocytogenes. HPE extracts showed antioxidant activity not only in ORAC but they were also able to prevent/attenuate peroxide-induced damage upon DNA. Moreover, on its own, HPE extract induced less oxidative damage than the control extract. Concerning the cytotoxicity, HPE extracts (at 0.5 and 1.0 mg/mL) were not harmful to HT29 cell lines, while control extracts (obtained at atmospheric pressure) at higher concentrations (>1.0 mg/mL) slightly reduced the metabolism of the cells. Finally, all extracts showed inhibition of the viability of 3 cancerous cell lines (>2.0 mg/mL for Caco-2, HeLa, and TR146) to below 15%.
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Due to the lack of innovative valorization strategies, berry pomaces are a poorly utilized as a cheap source of valuable nutrients and phytochemicals. An effective biorefining scheme was developed to recover functional components from lingonberry pomace by consecutive supercritical CO2 (SFE-CO2), pressurized liquid (PLE) and enzyme assisted (EAE) extractions. SFE-CO2 at optimized parameters yielded 11.8 g/100 g of lipophilic fraction, containing 43.3 and 37.4% of α-linolenic and linoleic fatty acids, respectively. The combined PLE with ethanol and water additionally recovered 61.8 g/100 g of polar constituents and reduced the antioxidant capacity of starting material by up to 94%. The major portion of the antioxidants (89-94% in different assays), anthocyanins (231 mg/100 g pomace) and proanthocyanidins (15.9 g/100 g pomace) was present in PLE-EtOH extract. Cyanidin-3-galactoside was the major anthocyanin (146.9 mg/100 g). High-pressure fractionation was more efficient for obtaining bioactive pomace constituents as compared with conventional and enzyme-assisted extractions.
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Fracionamento Químico/métodos , Solventes/química , Vaccinium vitis-Idaea , Gerenciamento de Resíduos/métodos , Antocianinas/análise , Antocianinas/química , Antioxidantes/análise , Antioxidantes/química , Dióxido de Carbono/química , Etanol/química , Frutas/química , Galactosídeos/análise , Galactosídeos/química , Química Verde , Resíduos Industriais , Complexos Multienzimáticos/química , Complexos Multienzimáticos/metabolismo , Pressão , Proantocianidinas/análise , Proantocianidinas/química , ÁguaRESUMO
Garlic-based extracts have been surveyed as healthy promoting supplements in relation to their content of organosulfur compounds. The present study investigated the effect of high pressure extraction and maceration, and four extraction solvents (three hydroalcoholic mixtures and sunflower oil) on the total and relative amounts of the main organosulfur compounds of clove and stem extracts of the Italian ecotype "Aglio Rosso di Sulmona" (Sulmona Red Garlic). Organosulfur compounds were more abundant in cloves than in stem extracts. High pressure extraction led to higher recoveries of hydrophilic compounds than maceration whereas the highest amounts of lipophilic compounds were detected in macerates. A higher alliin-to-allicin conversion ratio was detected in clove, in comparison to stem extracts. Sunflower oil fostered allicin decay to compounds such as ajoenes, vinyldithiins and sulfides. The use of specific extraction methods may lead to standardised grade extracts, both from native raw materials and by-products.
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Alho/química , Extratos Vegetais/química , Caules de Planta/química , Compostos de Enxofre/química , Ecótipo , Alho/genética , Interações Hidrofóbicas e Hidrofílicas , Pressão , SyzygiumRESUMO
The aim of this study was to use high hydrostatic pressure treatment to enhance the extraction efficiency of the active components from the fruiting bodies of Antrodia cinnamomea, and compare with those obtained by shake and ultrasonic extraction methods. The conditions of high pressure extraction (HPE) at 600 MPa, a liquid/solid ratio of 40:1, and 3 min of treatment yielded triterpenoids and adenosine concentrations of 410.41 mg/100 mL and 0.47 mg/100 mL, respectively, which did not differ significantly from those with the two other treatments-shake extraction at 180 rpm for 8 h and ultrasonic extraction at 50 Hz for 60 min. The HPE extracts significantly attenuated reactive oxygen species, nitric oxide and prostaglandin E2 production in lipopolysaccharide-stimulated RAW 264.7 cells than shake extracts did. SEM micrographs revealed that high-pressure caused physical morphological damage to the mycelium of fruiting bodies, such as distortion and disruption of mycelial cells, and increased the mass-transfer effectiveness of the solvent and solute. HPE can be employed as an efficient extraction technique for production of bioactive ingredients that might have a potential application in food and related industries.
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The hulls of Djulis (Chenopodium formosanum) are a type of agricultural waste. Using 70% ethanol as the extraction solvent, this study compared the extraction yields of high-pressure-assisted extraction (HPE) and conventional oscillation extraction (CE) for Djulis hulls (DH). The total phenolic and flavonoid contents, and antioxidant, anti-inflammatory and anti-tyrosinase activities were also compared. Our findings indicated that 600 MPa/5 min of HPE resulted in higher total phenolic (567-642 mg GAE/g) and flavonoid (47.2-57.2 mg QU/g) concentrations; gallic acid (44.5-53.2 µg/g) and rutin (26.8-34.2 µg/g) were the main phenolic and flavonoid compounds. When the extraction pressure was greater than 450 MPa, HPE extracts showed stronger antioxidant capacity and anti-tyrosinase activity than CE extracts. In a LPS-induced RAW 264.7 cell model of inflammation, HPE extracts had significant inhibitory effects on the cumulative concentrations of nitric oxide and prostaglandin E2. These results indicate that HPE had a better extraction yield, and required a shorter time for the extraction of functional ingredients from DH. Hence, DH could be a potential source for natural antioxidants for the food and biotechnology industries.
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Buriti fruit, with high content in carotenoids and antioxidant compounds, is well appreciated for its organoleptic characteristics. However, its shell, an agroindustrial residue, is mostly discarded. Therefore, to verify the technological potential of the buriti shells, the aim of this this study was to evaluated the antioxidant potential of the extracts from buriti shell obtained by pressurized liquid extraction (PLE) with ethanol/water mixtures. PLE optimization was performed by response surface methodology, with all results maximized at the conditions of 71.21⯰C and with 91.58% of ethanol. The yields values varied from 16.82 to 25.16%, total carotenoids from 23.38 to 1056.59⯵g ß-carotene equivalent g-1, total phenolic content from 143.37 to 172.02â¯mg Gallic acid equivalent g-1, DPPH from 31.04 to 48.62⯵g.mL-1, and ABTS from 1.87 to 2.70â¯mmol TEAC. g-1. Therefore, considering the lack of studies about buriti shell, the present work provides valuable results that confirm the PLE relevance to enhance the value of this neglected material.
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Antioxidantes/química , Arecaceae/química , Antioxidantes/isolamento & purificação , Arecaceae/metabolismo , Carotenoides/análise , Carotenoides/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Frutas/química , Frutas/metabolismo , Extração Líquido-Líquido , Fenóis/análise , Fenóis/isolamento & purificação , Extratos Vegetais/química , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
Ultra-high-pressure extraction combined with high-speed counter-current chromatography was employed to extract and purify wedelolactone and isodemethylwedelolactone from Ecliptae Herba. The operating conditions of ultra-high-pressure extraction were optimized using an orthogonal experimental design. The optimal conditions were 80% aqueous methanol solvent, 200 MPa pressure, 3 min extraction time and 1:20 (g/mL) solid-liquid ratio for extraction of wedelolactone and isodemethylwedelolactone. After extraction by ultra-high pressure, the extraction solution was concentrated and subsequently extracted with ethyl acetate; a total of 2.1 g of crude sample was obtained from 100 g of Ecliptae Herba. A two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (3:7:5:5, v/v) was used for high-speed counter-current chromatography separation, by which 23.5 mg wedelolactone, 6.8 mg isodemethylwedelolactone and 5.5 mg luteolin with purities >95% were purified from 300 mg crude sample in a one-step separation. This research demonstrated that ultra-high-pressure extraction combined with high-speed counter-current chromatography was an efficient technique for the extraction and purification of coumestans from plant material.
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Asteraceae/química , Cumarínicos/isolamento & purificação , Distribuição Contracorrente/métodos , Extratos Vegetais/química , Fracionamento Químico , Cumarínicos/análise , Cumarínicos/química , PressãoRESUMO
This study aimed to assess the effect of high pressure (300 and 600â¯MPa) and enzymatic extraction (pectinase and cellulase) on the phenolic compounds profile, antioxidant capacity and antimicrobial activity of extracts from pomegranate by-products. Antimicrobial activity against eight different strains of pathogenic and contaminant bacteria and against five beneficial bacteria including Lactobacillus and Bifidobacterium strains were determined. The maximum level of total phenolic content, as well as antioxidant capacity were observed at 300â¯MPa, however enzymatic extraction did not improve the extraction yields. Punicalagin isomers and bis-hexahydroxydiphenoyl-glucoside isomer were the most abundant phenolic compounds found in the extracts. All pomegranate peel extracts demonstrated selective antimicrobial activity against all pathogenic bacteria without affecting beneficial ones. Pressurized extracts presented lower minimum inhibitory concentration against Bacillus cereus and Pseudomonas aeruginosa and lower minimum bactericidal concentration against B. cereus, while, enzymatic extracts presented lower minimum bactericidal concentration for Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus and Listeria monocytogenes. Principal component analyses reveled that antioxidant activity and phenolic compounds content were strongly related with antimicrobial activity. Pomegranate peels extracts obtained by high pressure extraction could so be used as a source of high added-value bioactive compounds for antioxidant and antimicrobial applications.
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Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Lythraceae/química , Extratos Vegetais/farmacologia , Antibacterianos/isolamento & purificação , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Antioxidantes/análise , Bactérias/efeitos dos fármacos , Sequestradores de Radicais Livres , Frutas/química , Taninos Hidrolisáveis , Testes de Sensibilidade Microbiana , Fenóis/análise , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , PressãoRESUMO
C. sativa threshing residues were biorefined by consecutive supercritical carbon dioxide (SFE-CO2) pressurised liquid (PLE) and enzyme-assisted extractions (EAE). SFE-CO2 at optimised parameters yielded 8.3g/100g of lipophilic fraction containing 0.2 and 2.2g of cannabidiol and cannabidiolic acid per 100g of threshing residues, respectively. The recovery of cannabinoids from plant material was >93%. PLE gave 4.3 and 18.9g/100g of flavonoid-containing polar extracts, while EAE added 20.2% (w/w) of water-soluble constituents and increased the release of mono- and disaccharides by up to 94%. Antioxidant capacity of non-polar and polar fractions was in the range of 1.3-23.5mg gallic acid equivalents/g DW and 0.6-205.2mg Trolox equivalents/g DW, with the highest activities of PLE-EtOH/H2O extract. The combined SFE-CO2, PLE and EAE reduced antioxidant capacity of starting plant material by 90-99%, showing that suggested multistep fractionation procedure is efficient in the recovery of a major part of the antioxidatively active constituents from hemp threshing residues.
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Antioxidantes/isolamento & purificação , Biotecnologia/métodos , Canabinoides/isolamento & purificação , Cannabis/química , Fracionamento Químico/métodos , Antioxidantes/química , Canabidiol/isolamento & purificação , Dióxido de Carbono/química , Etanol/química , Extratos Vegetais/química , Solubilidade , Água/químicaRESUMO
In this study, the ultra-high pressure extraction (UHPE) conditions for obtaining the maximum flavonoid yield from Xinjiang jujube (Ziziphus jujuba Mill.) leaves (XJL) were optimized by response surface methodology (RSM). Boxâ»Behnken design (BBD) was applied to evaluate the effects of four variables (extraction temperature, pressure, time and liquid-to-solid ratio) on flavonoid yield. The results showed that the optimal flavonoid yield (25.45 ± 0.21 mg/g) was derived at 50.0 °C, 342.39 MPa, 11.56 min, and a liquid-to-solid ratio of 43.95 mL/g. Eight compounds were tentatively identified and quantified as kaempferol and quercetin glycosides with UPLC-ESI-MS. Compared to ultrasound-assisted extraction (UAE), UHPE can obtain higher concentrations of total flavonoids and stronger DPPH and ABTS radical-scavenging activities in a much shorter time. Therefore, UHPE is an alternative to UAE for obtaining flavonoids from XJL, which may be an optional method for large-scale industrial flavonoid extraction from XJL.
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Antioxidantes/farmacologia , Flavonoides/farmacologia , Extratos Vegetais/farmacologia , Folhas de Planta/química , Ziziphus/química , Antioxidantes/química , Antioxidantes/isolamento & purificação , Fracionamento Químico , Cromatografia Líquida , Flavonoides/química , Flavonoides/isolamento & purificação , Espectrometria de Massas , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificaçãoRESUMO
The extraction yield, phenolic content, anthocyanin content and antioxidant activity of extracts from different varieties of red grapes, Cabernet Sauvignon, Merlot, Petit Verdot, Syrah, Tempranillo and Tintilla, using pressurized green solvents have been analyzed. Two techniques were studied and compared: supercritical fluid extraction (SFE) with CO2 + 20% ethanol and pressurized liquid extraction (PLE) with either ethanol, water or an ethanol/water mixture as the extraction solvents. The Petit Verdot variety allowed the highest global and phenolic yield, and antioxidant activity. The best conditios for PLE obtained from the experimental design and kinetic study were 50% ethanol/water as the pressurized solvent at 90 bar, 120 °C, a flow rate of 5 g/min and, an extraction time of 90 min. A statistical analysis of variance has been performed and it was found that temperature is the only variable that has a statistical influence on the extraction yield. The antioxidant activity levels of the extracts are very promising and they are similar to those obtained with the antioxidant tocopherol.