The urban in ecology: a quantitative textual analysis of the scientific literature over a century.

Many authors have described 'urban ecology' as a subfield of ecology that emerged in the 1990s and has expanded exponentially. We propose to take a step further and analyse the expansion of the 'urban' in ecology with a novel quantitative approach, with the aim to better understand the relationship between ecology and the urban. Previous quantitative assessments of the urban in ecology have focused on short to medium time spans (5 to 40 years) and on research coined as 'urban ecology', and have rarely considered the content of publications (e.g., vocabulary and topics) using quantitative methods. In this paper, we conduct a bibliometric assessment and an in-depth quantitative textual data analysis of a corpus of 960 articles published from 1922 to 2018 in 10 leading English-language journals in ecology and conservation biology. We address the following questions: (1) When and how have urban environments been integrated into ecological research during the past century? (2) What urban research topics have been investigated in ecology during the same period? (3) How have these research topics changed through time? Our results show that the urban was never entirely absent from publications in ecology. The quantitative analyses highlight three turning points (1970s, 1990s and 2000s) in the relationship between ecology and the urban. Moreover, they help visualize the shift from particularly scattered publications at the beginning of the period to publications characterized by a more homogeneous vocabulary, reflecting the stabilization of a research field focused on the urban in ecology. Supplementary Information: The online version contains supplementary material available at 10.1007/s11252-024-01603-4.

Seaweed functional strategies, functional groups, and taxon dynamics through a 213-year historical series of Rio De Janeiro Bay.

From Darwin's visit to nowadays, we analyzed the changes in species- and genus-level richness and turnover, equity, diversity, composition, as well as functional group and functional strategy dynamics over a 213-year historical series of the seaweed assemblage from Rio de Janeiro Bay. We developed new analysis methods to serve as a protocol for worldwide functional and bioindicator assessment of seaweed assemblages. Over the two centuries of changes tracked, the integration of taxonomic, functional group, and functional strategy analyses unraveled a trajectory of seaweed assemblage restructuring under human disturbance. Our taxonomic analyses showed a high species and genus turnover and a decline in richness, with the local extinction of the Sargassum genus, responsible for warm temperate kelp forests in the Western Atlantic. Seaweed functional group data presented a trend of morphological simplification and miniaturization, with the decline of marine forest canopy and subcanopy-forming groups favoring filamentous and crustose algae dominance. Functional strategy data showed the historical decline of competitive structuring as an assemblage driver in favor of disturbance and stress-structuring, as highlighted by the competitive strategy decrease, coupled with stress-tolerant and ruderal strategies rise across metrics. These changes were successfully portrayed by the new ecological index we proposed, the C/SR index. It is the first quantitative index for seaweed assemblages, designed to be a trackable metric for conservation, monitoring, and restoration efforts.

Animal personality in multiple stressor environments: the evolutionary ecology of among-individual differences in responses to stressor suites.

Animal personality differences may have evolved as alternative strategies for negotiating multiple stressor landscapes. Indeed, ecologists are increasingly recognizing that interactions among multiple stressors can transform selective landscapes and behavioural and physiological responses to stress regimes. Yet, evaluating this hypothesis poses challenges, as most studies involving relationships between personality variation and the environment consider single stressors. Here, we review the literature to explore the theory and evidence that multiple stressor environments may mediate personality variation. We consider effects on evolution of personality variation, as influenced by life-history, energetic and behavioural trade-offs, and effects on phenotypic expression of personality traits. We then explore how personality variation may modulate behavioural and physiological responses to multiple stressors, and how differential responses may be affected by personality-dependent movement ecology and cognitive strategies. Among-individual differences in responses to multiple stressors are critical to elucidate, as multi-stress interactions may transform animal behavioural and physiological responses relative to those predicted under single stressor scenarios, and because among-individual variation comprises the basis for evolutionary shifts in stress responsiveness and population resiliency to global environmental change.

Crossed effects of helminth infection and lead exposure on fitness: An experimental study in feral pigeons (Columba livia).

Living organisms are exposed to multiple environmental factors that can affect their fitness. The negative effects of these simultaneous stressors can be additive or can interact in negative synergistic or antagonistic ways to affect the health of exposed individuals. Parasites can accumulate pollutants in their own tissues and have been shown to increase the tolerance of their hosts to different pollutants (antagonistic interaction between parasites and pollutants). Through an experimental approach, we tested the existence of combined antagonistic effects between intestinal parasites and lead exposure on urban feral pigeons (Columba livia) which are known to be exposed to trace metal pollution and harbour a wide variety of internal and external parasites. We experimentally exposed wild feral pigeons in captivity to two treatments: an anthelmintic treatment to eliminate intestinal nematode parasites; an exposure to lead for a period of 6 months. We tested the effects of these crossed treatments on several components of fitness: immunocompetence, reproduction, and body mass. Our findings suggest that the overall effects of lead exposure, either alone or in combination with the presence of intestinal parasites (without anthelmintic treatment) were negative, through either additive or synergistic means. Our results reveal the existence of negative combined effects between pollutant exposure and intestinal parasites, highlighting the importance of accounting for multiple stress factors when studying the effects of exposure to pollutants and/or other environmental stressors on the fitness of organisms.

Soil animal communities demonstrate simplification without homogenization along an urban gradient.

Urbanization profoundly impacts biodiversity and ecosystem function, exerting an immense ecological filter on the flora and fauna that inhabit it, oftentimes leading to simplistic and homogenous ecological communities. However, the response of soil animal communities to urbanization remains underexplored, and it is unknown whether their response to urbanization is like that of aboveground organisms. This study investigated the influence of urbanization on soil animal communities in 40 public parks along an urbanization gradient. We evaluated soil animal abundance, diversity, and community composition and related these measures to urban and soil characteristics at each park. The most urbanized parks exhibited reduced animal abundance, richness, and Shannon diversity. These changes were influenced by many variables underscoring the multifaceted influence of urbanization on ecological communities. Notably, contrary to our expectation, urbanization did not lead to community homogenization; instead, it acted stochastically, creating unique soil animal assemblages. This suggests that urban soil animal communities are concomitantly shaped by deterministic and stochastic ecological processes in urban areas. Our study highlights the intricate interplay between urbanization and soil animal ecology, challenging the notion of urban homogenization in belowground ecosystems and providing insight for managing and preserving belowground communities in urban areas.

The Saint-Leonard Urban Glaciotectonic Cave Harbors Rich and Diverse Planktonic and Sedimentary Microbial Communities.

The terrestrial subsurface harbors unique microbial communities that play important biogeochemical roles and allow for studying a yet unknown fraction of the Earth's biodiversity. The Saint-Leonard cave in Montreal City (Canada) is of glaciotectonic origin. Its speleogenesis traces back to the withdrawal of the Laurentide Ice Sheet 13,000 years ago, during which the moving glacier dislocated the sedimentary rock layers. Our study is the first to investigate the microbial communities of the Saint-Leonard cave. By using amplicon sequencing, we analyzed the taxonomic diversity and composition of bacterial, archaeal and eukaryote communities living in the groundwater (0.1 µm- and 0.2 µm-filtered water), in the sediments and in surface soils. We identified a microbial biodiversity typical of cave ecosystems. Communities were mainly shaped by habitat type and harbored taxa associated with a wide variety of lifestyles and metabolic capacities. Although we found evidence of a geochemical connection between the above soils and the cave's galleries, our results suggest that the community assembly dynamics are driven by habitat selection rather than dispersal. Furthermore, we found that the cave's groundwater, in addition to being generally richer in microbial taxa than sediments, contained a considerable diversity of ultra-small bacteria and archaea.

The effect of industrial and urban dust pollution on the ecophysiology and leaf element concentration of Tilia cordata Mill.

The influences of airborne trace elements in urban dust on element concentrations and functional traits of Tilia cordata were examined. For the present study, the unwashed and washed leaves of T. cordata were collected to assess the concentration of metals in Katowice City, Poland, from sites of different traffic intensity and industry activity. The content of Al, Cd, Cr, Cu, Fe, Mn, Pb, Zn, C, and N was measured. Additionally, a number of functional traits such as photosynthetic pigment content, specific leaf area (SLA), leaf dry matter content (LDMC), and diseased areas of the leaves were determined to assess the impact of the polluters on the physiology of the trees and their resources acquisition strategy. We hypothesized that the photosynthetic pigments of T. cordata will decrease with the traffic and industry intensity, and the traits related to the resources acquisition and stress resistance will shift into a more conservative strategy. The Principal Component Analysis and the Inverse Distance Weighting (IDW) interpolation method helped to identify that the Fe, Zn, Al, and Cr were related mainly to traffic intensification and Pb to industrial activities. The results indicate that Katowice is considerably polluted by Zn (up to 189.6 and 260.2 mg kg-1 in washed and unwashed leaves, respectively), Pb (up to 51.7 and 133.6 mg kg-1), and Cd (up to 2.27 and 2.43 mg kg-1) compared to other cities worldwide. Also, a reduction of approximately 27% in the photosynthetic pigments was observed at the high-traffic and industrial sites. The trees from the mainly affected areas with heavy traffic and industry tend to apply a conservative resources strategy with a decrement in SLA and an increment in LDMC. In contrast, the opposite trend was observed at the less affected sites (high SLA, low LDMC). The study showed that unfavourable urban conditions can trigger a plastic response on multiple levels. Knowledge of the possible paths of adaptation to urban conditions of different plant species is nowadays crucial to appropriate urban greenery planning.

Widespread introduced species dominate the urban tree assemblage on the endemic-rich tropical island of São Tomé.

The Afrotropics are experiencing some of the fastest urbanisation rates on the planet but the impact of city growth on their rich and unique biodiversity remains understudied, especially compared to natural baselines. Little is also known about how introduced species influence ß-diversity in these contexts, and how patterns coincide with native ranges of species. Here we investigated how tree assemblages of the endemic-rich Afrotropical island of São Tomé differed between urban, rural and natural zones. These were primarily characterised by urban greenspaces, shade plantations, and old-growth forests, respectively. Based on 81 transects, we assessed biodiversity metrics of endemic, native and introduced species. Tree abundance and species richness were highest in the natural zone, where the composition was most different from the urban zone. The tree community of the rural zone was the most uneven and had the least variation among transects, representing the lowest ß-diversity. The urban zone was dominated by introduced species (57.7%), while the natural zone hosted almost exclusively native species (93.3%), including many endemics (26.1%). The biogeographic realms that species originated from were particularly diverse in the urban zone, with few species from the Afrotropics. In contrast to native and endemic trees, introduced trees were clearly associated with urban and rural expansion, as they were much more abundant and species-rich in these zones than in the natural zone, facilitating biotic homogenisation. These findings highlight how urban and rural environments are affecting the native tree flora of São Tomé, and the need for conservation measures geared towards globally threatened and endemic tree species. Importantly, these require the protection of natural forests, despite the rising land demands for settlements and agriculture. Ultimately, such action to conserve endemic trees will contribute to global efforts to prevent further biodiversity declines.

Unveiling the urban colonization of the Asian water monitor (Varanus salvator) across its distribution range using citizen science.

Background: This study aims to investigate the urban colonization of the Asian water monitor (Varanus salvator) across its entire range of distribution, addressing the paucity of research on this species in urban ecosystems. The research spans the geographic range of the Asian water monitor, focusing on urbanized areas where the species accumulates more observations (Bangkok, Colombo, Jakarta, Kuala Lumpur and Singapore). Methods: We conducted a systematic review to comprehensively assess the current knowledge of the species' presence in cities. Additionally, citizen science data from repositories like GBIF (Global Biodiversity Information facility ) were utilized to analyze the distribution patterns of V. salvator in urban environments. To elucidate urban distribution and correct collection biases, observations were weighted by sampling effort, using as a proxy all squamate occurrences available from 2010-2023, including V. salvator. Results: Despite the widespread presence of the Asian water monitor in numerous cities within its distribution range, the available studies on the topic appear to be scarce. Existing research primarily consists of descriptive reports on diet and behavior. Our findings indicate that V. salvator predominantly colonizes green patches in urban areas, such as parks and small gardens. Larger cities exhibit higher records, potentially due to both permanent populations and increased citizen science reporting. Conclusions: The Asian water monitor, as the largest lizard with established populations in cities, remains scarcely studied on a broader scale. However, the urban design of each city seems relevant to understand the distribution patterns within each context. Our study highlights the need for further research to explore the ecological and human dimensions associated with the species' presence in urban environments.

Small towns limit dispersal and reduce genetic diversity in populations of Texas horned lizards.

There is a general expectation that urban populations will be fragmented and the movement of individuals will be restricted leading to low effective population sizes, low genetic diversity, higher inbreeding, and higher differentiation than populations living in more continuous habitat. In this study, we compare the genetic diversity and differentiation of Texas horned lizards that are found in four small towns (Kenedy, Karnes City, Rockdale, and Smithville) in Texas and at Tinker Air Force Base, Oklahoma to populations that occur in 16 natural areas and to an introduced population in South Carolina. We also present more detailed spatial genetic data and home range data for several of the towns. Texas horned lizards (Phrynosoma cornutum) living in small towns have lower genetic diversity, higher differentiation, and smaller effective population sizes than populations located in more natural areas. There was evidence for human-mediated movement of lizards into town; however, it has not been enough to counteract the effects of drift. Home range size is smaller in town than in more natural areas. Genetic patterns suggest dispersal occurs over short distances and is inhibited across areas with a high percent of impervious surface and major roads. These data suggest that effective planning to maintain suitable habitat and corridors to facilitate movement is critical to maintaining small terrestrial species like the Texas horned lizard and must be integrated into the early stages of urban development.

The impact of urbanization on health depends on the health metric, life stage and level of urbanization: a global meta-analysis on avian species.

Stressors associated with urban habitats have been linked to poor wildlife health but whether a general negative relationship between urbanization and animal health can be affirmed is unclear. We conducted a meta-analysis of avian literature to test whether health biomarkers differed on average between urban and non-urban environments, and whether there are systematic differences across species, biomarkers, life stages and species traits. Our dataset included 644 effect sizes derived from 112 articles published between 1989 and 2022, on 51 bird species. First, we showed that there was no clear impact of urbanization on health when we categorized the sampling locations as urban or non-urban. However, we did find a small negative effect of urbanization on health when this dichotomous variable was replaced by a quantitative variable representing the degree of urbanization at each location. Second, we showed that the effect of urbanization on avian health was dependent on the type of health biomarker measured as well as the individual life stage, with young individuals being more negatively affected. Our comprehensive analysis calls for future studies to disentangle specific urban-related drivers of health that might be obscured in categorical urban versus non-urban comparisons.

Socioeconomic Disparities in Urban Forest Diversity and Structure in Green Areas of Santiago de Chile.

Urban trees enhance biodiversity, provide ecosystem services, and improve quality of life in cities. Despite their benefits, trees are not distributed equitably, and many cities exhibit a "luxury effect". Given the importance of public green space for providing access to urban tree benefits, we investigated the relationship between socioeconomic level and tree diversity and structure in 60 green areas in Santiago de Chile. Species richness and total tree abundance did not significantly vary among socioeconomic levels; however, a differential effect was found according to species origin. Introduced tree species exhibited similar abundance and species richness across socioeconomic levels, but native tree species were more abundant and richer in higher socioeconomic level areas compared to lower ones. Tree cover was higher in the high and medium socioeconomic level areas than in the low socioeconomic level area. A higher average DBH was found in the medium socioeconomic level area, which may be explained by older neighborhoods and a legacy of the luxury effect. Our findings reveal that socioeconomic groups are associated with differences in tree cover, width, and the number of native species in public green areas. Consequently, urban residents have different provisions of ecosystem services and opportunities to interact with natural heritage. Increasing the amount of tree cover and native species available to vulnerable groups will reduce disparities.

Public safety considerations constraint the conservation of large old trees and their crucial ecological heritage in urban green spaces.

Large old trees in urban public green spaces deliver a diversity of values essential for human well-being, including biodiversity conservation. Yet, the conservation of large old trees bearing key wildlife microhabitats interferes with safety considerations. This intuitive notion, however, is backed by an insufficient and scattered body of evidence. Here, we empirically examined this process using data on 5974 trees across 510 sample plots, organized as quintuplets within 102 sample sites, including urban parks, cemeteries, recreational forests, and historic reserves in the urban agglomeration of Kraków, Poland. Our analyses demonstrate that trees situated in areas frequently visited by people, or those near walking paths, benches, or playgrounds, have elevated accident hazards and, therefore, necessitate intensive tree surgeries (pruning and logging) to remain harmless. Large old trees, which bear the most diverse microhabitats and pose greater risks when they collapse, are especially affected by these measures. Accordingly, we found that the co-occurrence of large trees with elevated accident hazards results in significant losses of dead and sloped trees, and trees with cavities, injuries, crown deadwood, fungal fruiting bodies, or epiphytes, particularly in parks and, to a lesser extent, in recreational forests. Apparently, some tree-related microhabitats, such as injuries, cavities, and microsoils, also emerge in risky spots after pruning. Our findings underscore that the conservation of large old trees and their ecological functions faces significant challenges due to safety considerations. To address conservation challenges and harmonize human coexistence with biodiversity, we recommend enhancing environmental awareness and reevaluating arboricultural and planning policies. This would involve establishing strategic and pocket reserves on city peripheries and interiors, allowing larger older trees to thrive and develop important microhabitats without compromising public safety. Otherwise, we risk losing many large old trees and/or their superior value for wildlife, which will regenerate over decades, if not centuries.

Exploring People's Perception on Pros and Cons of Human-Bat Coexistence in Urban Environs in Southwestern Nigeria.

This research intricately explores the dynamics surrounding the coexistence of humans and roosting bats in urban areas, meticulously examining both the advantageous and detrimental aspects of their living arrangement. The study conducted a comprehensive survey with 286 residents in Iwo and Ogbomoso, where Eidolon helvum bats are known to roost, generating a robust dataset for thorough analysis. Rigorous statistical assessments, including the KMO and Bartlett's tests, confirmed the data's reliability at a significance level of P < .05. The respondent demographic revealed a predominance of 65% male participants, with an overwhelming 85% claiming familiarity with bats in their respective domains. Utilizing factor analysis, the study identified 8 salient variables from the initial 26, shedding light on diverse perceptions regarding bats: (i) Urban roosting (16.729%); (ii) Impact on tree growth (12.607%); (iii) Failed dislodgement attempts (11.504%); (iv) Medicinal value (10.240%); (v) Co-habitation preference (9.963%); (vi) Costly dislodgment consequences (9.963%); (vii) Beautification disruption (5.615%); and (viii) Structure defacement (5.510%). These factors were systematically categorized into 4 distinct themes: (A) Forced cohabitation (26.762%); (B) Environmental degradation by bats (23.732%); (C) Consequences of dislodging bats (21.477%); and (D) Acknowledged benefits of bats (10.240%). Co-habitation with bats becomes a necessity for ecological balance and, importantly, to safeguard the livelihood of roosting bats within their natural ecology, which man has encroached upon through urbanization, making all negatives arising from such existence self-inflicted by man. However, this study underscores the importance of human-bat cohabitation for mutual benefits, emphasizing potential detrimental consequences, including significant costs, associated with displacing bats from their natural ecosystem. These consequences may exacerbate the impacts of climate change, environmental degradation, and ecological imbalance. Further research is recommended to explore the positive aspects of the sustainable roosting bats' existence in the natural environment.

The Impact of Wildflower Habitat on Insect Functional Group Abundance in Turfgrass Systems.

Urbanization is rapidly influencing the abundance and diversity of arthropods. Within urban systems, managed turfgrass is a prominent land cover which can support only a limited number of arthropod groups. To allow for more arthropod biodiversity and to support beneficial insects within turfgrass, increasing numbers of land managers are choosing to partially convert turf habitat to wildflower habitat using commercially available seed mixes. However, the population dynamics of arthropod groups in these systems are poorly known, with consequentially little information on best long-term practices for managing wildflower habitats in turfgrass systems. To address this gap, we sampled insects using pan traps in turfgrass systems pre- and post-implementation of wildflower habitats and examined the change in abundance of several insect families and functional guilds. Insect groups had variable responses to wildflower habitat implementation, with some groups such as sweat bees and skipper butterflies showing a decline two years post-implementation. Other groups, such as predatory flies, were relatively more abundant one and two years post-implementation. These variable responses point to the need for more research on the long-term effects of wildflower habitats on beneficial insects in turfgrass habitats.

Microplastic atmospheric pollution in an urban Southern Brazil region: What can spider webs tell us?

The World Health Organization categorizes air pollution as the presence of one or more contaminants in the atmosphere such as smoke, dust, and particulate matter like microplastics, which are considered a priority pollutant. However, only a few studies have been developed on atmospheric pollution, and knowledge about MPs in the atmosphere is still limited. Spider webs have been tested and used as a passive sampling approach to study anthropogenic pollution. Despite this, studies on microplastic contamination using spiderwebs as samplers are scarce. Thus, this study uses spider webs as passive indicators to investigate air quality regarding microplastic contamination in an urbanized area. Therefore, 30 sampling points were selected, and webs of Nephilingis cruentata were collected. The spider webs were dipped in KOH 10 %. After digestion, the solution was washed and sieved through a 90 µm geological sieve. The remaining material was transferred to a Petri dish with filter paper, quantified, and identified by type and color. The chemical composition of the polymers was determined using Raman spectroscopy. 3138 microplastics were identified (2973 filaments and 165 fragments). The most frequent colors were blue and black. Raman spectroscopy revealed five types of polymers: Isotactic Polypropylene, Polyethylene Terephthalate, Polyurethane, Polyamide, and Direct Polyethylene.

'Ripple effects' of urban environmental characteristics on cognitive performances in Eurasian red squirrels.

Urban areas are expanding exponentially, leading more species of wildlife living in urban environments. Urban environmental characteristics, such as human disturbance, induce stress for many wildlife and have been shown to affect some cognitive traits, such as innovative problem-solving performance. However, because different cognitive traits have common cognitive processes, it is possible that urban environmental characteristics may directly and indirectly affect related cognitive traits (the ripple effect hypothesis). We tested the ripple effect hypothesis in urban Eurasian red squirrels residing in 11 urban areas that had different urban environmental characteristics (direct human disturbance, indirect human disturbance, areas of green coverage and squirrel population size). These squirrels were innovators who had previously repeatedly solved a food extraction task (the original task). Here, we examined whether and how urban environmental characteristics would directly and indirectly influence performance in two related cognitive traits, generalisation and (long-term) memory. The generalisation task required the innovators to apply the learned successful solutions when solving a similar but novel problem. The memory task required them to recall the learned solution of the original task after an extended period of time. Some of the selected urban environmental characteristics directly influenced the task performance, both at the population level (site) and at individual levels. Urban environmental characteristics, such as increased direct and indirect human disturbance, decreased the proportion of success in solving the generalisation task or the memory task at the population (site) level. Increased direct human disturbance and less green coverage increased the solving efficiency at individual levels. We also found an indirect effect in one of the urban environmental characteristics, indirect human disturbance, in the generalisation task, but not the memory task. Such an effect was only seen at the individual level but not at the population level; indirect human disturbance decreased the first original latency, which then decreased the generalisation latency across successes. Our results partially support the ripple effect hypothesis, suggesting that urban environmental characteristics are stressors for squirrels and have a greater impact on shaping cognitive performance than previously shown. Together, these results provide a better understanding of cognitive traits that support wildlife in adapting to urban environments.

Online toolkits for collaborative and inclusive global research in urban evolutionary ecology.

Urban evolutionary ecology is inherently interdisciplinary. Moreover, it is a field with global significance. However, bringing researchers and resources together across fields and countries is challenging. Therefore, an online collaborative research hub, where common methods and best practices are shared among scientists from diverse geographic, ethnic, and career backgrounds would make research focused on urban evolutionary ecology more inclusive. Here, we describe a freely available online research hub for toolkits that facilitate global research in urban evolutionary ecology. We provide rationales and descriptions of toolkits for: (1) decolonizing urban evolutionary ecology; (2) identifying and fostering international collaborative partnerships; (3) common methods and freely-available datasets for trait mapping across cities; (4) common methods and freely-available datasets for cross-city evolutionary ecology experiments; and (5) best practices and freely available resources for public outreach and communication of research findings in urban evolutionary ecology. We outline how the toolkits can be accessed, archived, and modified over time in order to sustain long-term global research that will advance our understanding of urban evolutionary ecology.

Bacterial pathogens in Ixodes ricinus collected from lizards Lacerta agilis and Zootoca vivipara in urban areas of Wroclaw, SW Poland- preliminary study.

The aim of this study was to determine the level of infection of Ixodes ricinus ticks with pathogens (Borrelia spp., Rickettsia spp., and Anaplasma spp.) collected from Lacerta agilis and Zootoca vivipara lizards in the urban areas of Wroclaw (SW Poland). The study was carried out in July-August 2020. Lizards were caught by a noose attached to a pole or by bare hands, identified by species, and examined for the presence of ticks. Each lizard was then released at the site of capture. Ticks were removed with tweezers, identified by species using keys, and molecular tests were performed for the presence of pathogens. From 28 lizards (17 specimens of Z. vivipara and 11 specimens of L. agilis) a total of 445 ticks, including 321 larvae and 124 nymphs, identified as I. ricinus were collected. A larger number of ticks were obtained from L. agilis compared to Z. vivipara. Molecular tests for the presence of pathogens were performed on 445 specimens of I. ricinus. The nested PCR method for the fla gene allowed the detection of Borrelia spp. in 9.4% of ticks, and it was higher in ticks from L. agilis (12.0%) than from Z. vivipara (1.0%). The RFLP method showed the presence of three species, including two belonging to the B. burgdorferi s.l. complex (B. lusitaniae and B. afzelii), and B. miyamotoi. The overall level of infection of Rickettsia spp. was 19.3%, including 27.2% in ticks collected from Z. vivipara and 17.0% from L. agilis. Sequencing of randomly selected samples confirmed the presence of R. helvetica. DNA of Anaplasma spp. was detected only in one pool of larvae collected from L. agilis, and sample sequencing confirmed the presence of (A) phagocytophilum. The research results indicate the important role of lizards as hosts of ticks and their role in maintaining pathogens in the environment including urban agglomeration as evidenced by the first recorded presence of (B) miyamotoi and (A) phagocytophilum in I. ricinus ticks collected from L. agilis. However, confirmation of the role of sand lizards in maintaining (B) miyamotoi and A. phagocytophilum requires more studies and sampling of lizard tissue.

Novel habitats for biodiversity? A systematic review and meta-analysis of freshwater biodiversity in stormwater management ponds.

Stormwater ponds are increasingly becoming a dominant pond type in cities experiencing urban sprawl. These human-made ponds are designed primarily to control flooding issues associated with increased impervious surface in cities and serve to retain sediment and contaminants before flowing to urban downstream waterways. Along with these important functions, constructed ponds including stormwater ponds may be critical in urban freshwater conservation because they often represent some of the few remaining lentic environments (still water; e.g. ponds, wetlands, lakes) in many cities. We currently lack a clear understanding of the role that stormwater ponds play in serving as habitat for freshwater biodiversity. Here, we examined whether stormwater ponds support freshwater biodiversity in cities by reviewing the empirical literature on biotic community responses in urban stormwater ponds across a range of taxonomic groups. We conducted a meta-analysis on empirical papers that quantitatively examined differences in taxonomic richness between stormwater ponds and reference ponds (n = 11 papers, 22 effects). We also examined a broader set of 58 papers to qualitatively synthesize studies on stormwater pond communities and assess various indicators of habitat quality in stormwater ponds. In the studies examined, heterogeneity exists in the habitat quality of stormwater ponds and increased pollutant loads are often reported. However, the results highlight that stormwater ponds tend to contain alpha diversity comparable to reference ponds, and that overall, a range of ecologically important wildlife make use of and inhabit urban stormwater ponds. We find that stormwater ponds can often support communities with broad compositions of taxa, including those that are sensitive or vulnerable to environmental change. We compile recommendations provided within the studies in order to improve our understanding of the management of urban stormwater ponds for biodiversity conservation.