RESUMO
Endocrine disruptors such as bisphenol A (BPA) adversely affect the environment and human health. Laccases are used for the efficient biodegradation of various persistent organic pollutants in an environmentally safe manner. However, the direct application of free laccases is generally hindered by short enzyme lifetimes, non-reusability, and the high cost of a single use. In this study, laccases were immobilized on a novel magnetic three-dimensional poly(ethylene glycol) diacrylate (PEGDA)-chitosan (CS) inverse opal hydrogel (LAC@MPEGDA@CS@IOH). The immobilized laccase showed significant improvement in the BPA degradation performance and superior storage stability compared with the free laccase. 91.1% of 100 mg/L BPA was removed by the LAC@MPEGDA@CS@IOH in 3 hr, whereas only 50.6% of BPA was removed by the same amount of the free laccase. Compared with the laccase, the outstanding BPA degradation efficiency of the LAC@MPEGDA@CS@IOH was maintained over a wider range of pH values and temperatures. Moreover, its relative activity of was maintained at 70.4% after 10 cycles, and the system performed well in actual water matrices. This efficient method for preparing immobilized laccases is simple and green, and it can be used to further develop ecofriendly biocatalysts to remove organic pollutants from wastewater.
Assuntos
Compostos Benzidrílicos , Enzimas Imobilizadas , Lacase , Fenóis , Polietilenoglicóis , Poluentes Químicos da Água , Lacase/química , Lacase/metabolismo , Fenóis/química , Poluentes Químicos da Água/química , Enzimas Imobilizadas/química , Enzimas Imobilizadas/metabolismo , Polietilenoglicóis/química , Quitosana/química , Hidrogéis/química , Biodegradação Ambiental , Disruptores Endócrinos/químicaRESUMO
Despite their significant impact, comprehensive screenings and detailed analyses of per- and polyfluoroalkyl substance (PFAS) binding strengths at the orthosteric and allosteric sites of NRs are currently lacking. This study addresses this gap by focusing on the binding interaction analysis of both common and uncommon PFAS with the nuclear receptors (NRs) vitamin D receptor (VDR), peroxisome proliferator-activated receptor gamma (PPARγ), pregnane X receptor (PXR), and estrogen receptor alpha (ERα). Advanced docking simulations were used to screen 9507 PFAS chemicals at the orthosteric and allosteric sites of PPARγ, PXR, VDR, and ERα. All receptors exhibited strong binding interactions at the orthosteric and allosteric site with a significant number of PFAS. We verified the accuracy of the docking protocol through multiple docking controls and validations. A mixture modeling analysis indicates that PFAS can bind in various combinations with themselves and endogenous ligands simultaneously, to disrupt the endocrine system and cause carcinogenic responses. These findings reveal that PFAS can interfere with nuclear receptor activity by displacing endogenous or native ligands by binding to the orthosteric and allosteric sites. The purpose of this study is to explore the mechanisms through which PFAS exert their endocrine-disrupting effects, potentially leading to more targeted therapeutic strategies. Importantly, this study is the first to explore the binding of PFAS at allosteric sites and to model PFAS mixtures at nuclear receptors. Given the high concentration and persistence of PFAS in humans, this study further emphasizes the urgent need for further research into the carcinogenic mechanisms of PFAS and the development of therapeutic strategies that target nuclear receptors.
Assuntos
Fluorocarbonos , Simulação de Acoplamento Molecular , Ligação Proteica , Receptores Citoplasmáticos e Nucleares , Receptores Citoplasmáticos e Nucleares/metabolismo , Receptores Citoplasmáticos e Nucleares/química , Humanos , Fluorocarbonos/química , Fluorocarbonos/metabolismo , Sítios de Ligação , Ligantes , Sítio Alostérico , Receptor de Pregnano X/metabolismo , Receptor de Pregnano X/química , Disruptores Endócrinos/química , Disruptores Endócrinos/metabolismo , Disruptores Endócrinos/farmacologia , Receptores de Calcitriol/metabolismo , Receptores de Calcitriol/químicaRESUMO
In this study, we examined multiple endocrine-disrupting ultraviolet-absorbing compounds (UVACs) in marine invertebrates used in personal care products and packaging. Modified QuEChERS and liquid chromatography UniSpray ionization tandem mass spectrometry were used to identify 16 UVACs in marine invertebrates. Matrix-matched calibration curves revealed high linearity (r ≥ 0.9929), with limits of detection and quantification of 0.006-1.000 and 0.020-3.000 ng/g w.w., respectively. In oysters, intraday and interday analyses revealed acceptable accuracy (93%-120%) and precision (≤18%), except for benzophenone (BP) and ethylhexyl 4-(dimethylamino) benzoate. Analysis of 100 marine invertebrate samples revealed detection frequencies of 100%, 98%, 89%, 64%, and 100% for BP, 4-hydroxybenzophenone, 4-methylbenzophenone, 4-methylbenzylidene camphor, and benzophenone-3 (BP-3), respectively. BP and BP-3 were detected at concentrations of 4.40-27.39 and < 0.020-0.560 ng/g w.w., respectively, indicating their widespread presence. Overall, our proposed method successfully detected UVACs in marine invertebrates, raising concerns regarding their potential environmental and health effects.
Assuntos
Espectrometria de Massas em Tandem , Animais , Protetores Solares/química , Protetores Solares/análise , Disruptores Endócrinos/análise , Disruptores Endócrinos/química , Organismos Aquáticos/química , Organismos Aquáticos/efeitos da radiação , Benzofenonas/análise , Benzofenonas/química , Invertebrados/química , Contaminação de Alimentos/análise , Cromatografia Líquida de Alta Pressão , Raios Ultravioleta , Cromatografia LíquidaRESUMO
The Liquid Organic Hydrogen Carrier (LOHC) technology offers a technically attractive way for hydrogen storage. If LOHC systems were to fully replace liquid fossil fuels, they would need to be handled at the multi-million tonne scale. To date, LOHC systems on the market based on toluene or benzyltoluene still offer potential for improvements. Thus, it is of great interest to investigate potential LOHCs that promise better performance and environmental/human hazard profiles. In this context, we investigated the acute aquatic toxicity of oxygen-containing LOHC (oxo-LOHC) systems. Toxic Ratio (TR) values of oxo-LOHC compounds classify them baseline toxicants (0.1 < TR < 10). Additionally, the mixture toxicity test conducted with D. magna suggests that the overall toxicity of a benzophenone-based system can be accurately predicted using a concentration addition model. The estimation of bioconcentration factors (BCF) through the use of the membrane-water partition coefficient indicates that oxo-LOHCs are unlikely to be bioaccumulative (BCF < 2000). None of the oxo-LOHC compounds exhibited hormonal disrupting activities at the tested concentration of 2 mg/L in yeast-based reporter gene assays. Therefore, the oxo-LOHC systems seem to pose a low level of hazard and deserve more attention in ongoing studies searching for the best hydrogen storage technologies.
Assuntos
Daphnia , Estrogênios , Poluentes Químicos da Água , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/química , Humanos , Estrogênios/toxicidade , Estrogênios/química , Daphnia/efeitos dos fármacos , Animais , Bioacumulação , Androgênios/toxicidade , Androgênios/química , Disruptores Endócrinos/toxicidade , Disruptores Endócrinos/química , Hidrogênio/químicaRESUMO
A class of chemical with a potentially important perceived future contribution to the net zero carbon goal (as "green" solvents) is the methylimidazolium ionic liquids (MILs). These solvents are used in industrial processes such as biofuel production yet little is known about their environmental stability or toxicity in man although one MIL - 1-octyl-3-methylimidazolium (M8OI) - has been shown to activate the human estrogen receptor alpha (ERα). The stabilities of the chloride unsubstituted methylimidazolium (MI) and MILs possessing increasing alkyl chain lengths (2C, 1-ethyl-3-methylimidazolium (EMI); 4C, 1-butyl-3-methylimidazolium (BMI); 6C; 1-hexyl-3-methylimidazolium (HMI), 8C, M8OI; 10C, 1-decyl-3-methylimidazolium (DMI)) were examined in river water and a human liver model system. The MILs were also screened for their abilities to activate the human ERα in vitro and induce uterine growth in pre-pubertal rats in vivo. Short chain MILs (EMI, BMI and HMI) underwent negligible metabolism and mineralisation in river water; were not metabolised in a model of human liver metabolism; activated the human ERα in vitro and were estrogenic in vivo in rats. A structure-based computational approach predicted short chain MIL binding to both the estrogen binding site and an additional site on the human estrogen receptor alpha. Longer chain MILs (M8OI and DMI) were metabolised in river water and partially mineralised. Based on structure-activity considerations, some of these environmentally-derived metabolites may however, remain a hazard to the population. MILs therefore have the potential to become forever chemicals with adverse effects to both man, other animals and the environment in general.
Assuntos
Disruptores Endócrinos , Receptor alfa de Estrogênio , Imidazóis , Líquidos Iônicos , Líquidos Iônicos/toxicidade , Líquidos Iônicos/química , Imidazóis/toxicidade , Imidazóis/química , Disruptores Endócrinos/toxicidade , Disruptores Endócrinos/química , Humanos , Receptor alfa de Estrogênio/metabolismo , Animais , Ratos , FemininoRESUMO
Laundry wastewater is a significant source of nonylphenol ethoxylate (NPEO) at wastewater treatment plants, where its breakdown forms persistent nonylphenol (NP). NP poses risks as an endocrine disruptor in wildlife and humans. This study investigates the degradation of NPEO and COD in industrial laundry wastewater (LWW) using a two-stage process combining ultrafiltration (UF) and electro-oxidation (EO). UF was used to remove suspended solids, while soluble COD (COD0 = 239 ± 6 mg.L-1) and NPEO (NPEO0 = 341 ± 8 µg.L-1) were oxidized by the EO process. Different operating parameters were studied such as current density, electrolysis time, type of cathode and supporting electrolyte concentration. Using an experimental design methodology, the optimal conditions for COD and NPEO3-17 degradation were recorded. This included achieving 97% degradation of NPEO3-17 and 61% degradation of COD, with a total operating cost of 3.65 USD·m-3. These optimal conditions were recorded at a current density of 15 mA cm-2 for a 120-min reaction period in the presence of 4 g·Na2SO4 L-1 using a graphite cathode. The EO process allowed for reaching the guidelines required for water reuse (NPEO <200 µg.L-1, COD <100 mg.L-1) in the initial laundry washing cycles. Furthermore, our results demonstrate that both NP and NPEO compounds, including higher and shorter ethoxylate chains (NPEO3-17), were effectively degraded during the EO process, with removal efficiencies between 94% and 98%. This confirms the EO process's capability to effectively degrade NP, the by-product of NPEO breakdown.
Assuntos
Etilenoglicóis , Oxirredução , Ultrafiltração , Eliminação de Resíduos Líquidos , Águas Residuárias , Poluentes Químicos da Água , Águas Residuárias/química , Poluentes Químicos da Água/química , Eliminação de Resíduos Líquidos/métodos , Ultrafiltração/métodos , Etilenoglicóis/química , Análise da Demanda Biológica de Oxigênio , Fenóis/química , Disruptores Endócrinos/química , LavanderiaRESUMO
Bisphenol A (BPA), an endocrine-disrupting contaminant, is ubiquitous in the environment due to its presence in plastics, wastewater, and agricultural runoff. This study investigated the photodegradation behavior of BPA in coastal aquaculture waters near Qingdao, China. Lower salinity promoted BPA photodegradation, while higher salinity has an inhibitory effect, suggesting slower degradation in seawater compared to ultrapure water. Triplet-excited dissolved organic matter (3DOM*) was identified as the primary mediator of BPA degradation, with additional contributions from hydroxyl radicals (â¢OH), singlet oxygen (1O2), and halogen radicals (HRS). Alepocephalidae aquaculture water exhibited the fastest degradation rate, likely due to its high DOM and nitrate/nitrite (NO3-/NO2-) content, which are sources of 3DOM* and â¢OH. A positive correlation existed between NO3-/NO2- concentration and the BPA degradation rate. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) analysis identified the primary BPA photodegradation products, formed mainly through oxidative degradation, hydroxyl substitution, nitration, and chlorination pathways. Elucidating these photodegradation mechanisms provides valuable insights into the environmental fate and potential ecological risks of BPA in aquaculture environments. This knowledge can inform strategies for marine environmental protection and the development of sustainable practices.
Assuntos
Aquicultura , Compostos Benzidrílicos , Disruptores Endócrinos , Fenóis , Fotólise , Água do Mar , Poluentes Químicos da Água , Compostos Benzidrílicos/química , Fenóis/química , Fenóis/análise , Poluentes Químicos da Água/química , Poluentes Químicos da Água/análise , Disruptores Endócrinos/química , Disruptores Endócrinos/análise , China , Água do Mar/química , SalinidadeRESUMO
Phenolic environmental endocrine-disrupting chemicals (PEDCs) are persistent EDCs that are widely found in food packaging materials and environmental media and seriously threaten human health and ecological security. Human estrogen-related receptor γ (hERRγ) has been proposed as a mediator for the low-dose effects of many environmental PEDCs; however, the atomic-level descriptions of dynamical structural features and interactions of hERRγ and PEDCs are still unclarified. Herein, how three PEDCs, 4-(1-methylpropyl)phenol (4-sec-butylphenol), 5,6,7,8-tetrahydro-2-naphthol (tetrahydro-2-napthol), and 2,2-bis(4-hydroxy-3,5-dimethoxyphenyl)propane (BP(2,2)(Me)), interact with hERRγ to produce its estrogenic disruption effects was studied. Molecular docking and multiple molecular dynamics (MD) simulations were first conducted to distinguish the detailed interaction pattern of hERRγ with PEDCs. These binding structures revealed that residues around Leu271, Leu309, Leu345, and Phe435 are important when binding with PEDCs. Furthermore, the binding energies of PEDCs with hERRγ were also characterized using the molecular mechanics/Poisson Boltzmann surface area (MM-PBSA) and solvated interaction energy (SIE) methods, and the results showed that the interactions of CH-π, π-π, and hydrogen bonds are the major contributors for hERRγ binding to these three PEDCs. What is striking is that the methoxide groups of BP(2,2)(Me), as hydrophobic groups, can help to reduce the binding energy of PEDCs binding with hERRγ. These results provide important guidance for further understanding the influence of PEDCs on human health problems.
Assuntos
Disruptores Endócrinos , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Ligação Proteica , Humanos , Disruptores Endócrinos/química , Disruptores Endócrinos/metabolismo , Fenóis/química , Fenóis/metabolismo , Receptores de Estrogênio/metabolismo , Receptores de Estrogênio/química , Sítios de Ligação , Ligação de HidrogênioRESUMO
Endocrine-disrupting chemicals (EDCs) can disrupt the normal functioning of the endocrine system in organisms, leading to various health issues. Therefore, monitoring EDCs in the environment and food is of significant importance. In this study, a hydroxyl-functionalized ionic porous organic polymer (OH-IPOP) has been synthesized for the first time using 2-benzimidazolemethanol as a monomer. The OH-IPOP exhibited excellent adsorption performance towards phenolic EDCs. An efficient method for determination of phenolic EDCs (p-tert-butylphenol, bisphenol B, bisphenol A and bisphenol F) in environmental water and snapper samples was successfully established by with OH-IPOP as solid-phase extraction sorbent and determination with high-performance liquid chromatography-ultraviolet detection. The method showed good linearity (r2 > 0.998), low detection limits (0.008-0.020 ng mL-1 for lake water, 1.00-3.00 ng/g for snapper), high recovery rates (82.3-106 %), and good precision (relative standard deviation < 6.6 %), making it a highly efficient adsorbent for the enrichment of EDCs in complex sample matrices.
Assuntos
Disruptores Endócrinos , Fenóis , Polímeros , Poluentes Químicos da Água , Disruptores Endócrinos/análise , Disruptores Endócrinos/química , Disruptores Endócrinos/isolamento & purificação , Poluentes Químicos da Água/química , Poluentes Químicos da Água/análise , Fenóis/química , Fenóis/análise , Polímeros/química , Porosidade , Adsorção , Cromatografia Líquida de Alta Pressão , Extração em Fase Sólida/métodos , Extração em Fase Sólida/instrumentação , Animais , Cátions/química , Peixes , Contaminação de Alimentos/análise , Alimentos Marinhos/análiseRESUMO
Lignin-derivable bisguaiacols/bissyringols are viable alternatives to commercial bisphenols; however, many bisguaiacols/bissyringols (e.g., bisguaiacol F [BGF]) have unsubstituted bridging carbons between the aromatic rings, making them more structurally similar to bisphenol F (BPF) than bisphenol A (BPA) - both of which are suspected endocrine disruptors. Herein, we investigated the estrogenic activity (EA) and developmental toxicity of dimethyl-substituted bridging carbon-based lignin-derivable bisphenols (bisguaiacol A [BGA] and bissyringol A [BSA]). Notably, BSA showed undetectable EA at seven test concentrations (from 10-12 M to 10-6 M) in the MCF-7 cell proliferation assay, whereas BPA had detectable EA at five concentrations (from 10-10 M to 10-6 M). In silico results indicated that BSA had the lowest binding affinity with estrogen receptors. Moreover, in vivo chicken embryonic assay results revealed that lignin-derivable monomers had minimal developmental toxicity vs. BPA at environmentally relevant test concentrations (8.7-116 µg/kg). Additionally, all lignin-derivable compounds showed significantly lower expression fold changes (from â¼1.81 to â¼4.41) in chicken fetal liver tests for an estrogen-response gene (apolipoprotein II) in comparison to BPA (fold change of â¼11.51), which was indicative of significantly reduced estrogenic response. Altogether, the methoxy substituents on lignin-derivable bisphenols appeared to be a positive factor in reducing the EA of BPA alternatives.
Assuntos
Compostos Benzidrílicos , Estrogênios , Lignina , Fenóis , Animais , Fenóis/toxicidade , Fenóis/química , Humanos , Compostos Benzidrílicos/toxicidade , Compostos Benzidrílicos/química , Lignina/química , Embrião de Galinha , Estrogênios/toxicidade , Estrogênios/química , Células MCF-7 , Disruptores Endócrinos/toxicidade , Disruptores Endócrinos/química , Proliferação de Células/efeitos dos fármacos , Receptores de Estrogênio/metabolismo , Receptores de Estrogênio/efeitos dos fármacos , GalinhasRESUMO
This study reports the first application of in silico methods to assess the toxicity of 4-chloromethcathinone (4-CMC), a novel psychoactive substance (NPS). Employing advanced toxicology in silico tools, it was possible to predict crucial aspects of the toxicological profile of 4-CMC, including acute toxicity (LD50), genotoxicity, cardiotoxicity, and its potential for endocrine disruption. The obtained results indicate significant acute toxicity with species-specific variability, moderate genotoxic potential suggesting the risk of DNA damage, and a notable cardiotoxicity risk associated with hERG channel inhibition. Endocrine disruption assessment revealed a low probability of 4-CMC interacting with estrogen receptor alpha (ER-α), suggesting minimal estrogenic activity. These insights, derived from in silico studies, are critical in advancing the understanding of 4-CMC properties in forensic and clinical toxicology. These initial toxicological findings provide a foundation for future research and aid in the formulation of risk assessment and management strategies in the context of the use and abuse of NPSs.
Assuntos
Simulação por Computador , Psicotrópicos , Psicotrópicos/toxicidade , Psicotrópicos/química , Humanos , Animais , Cardiotoxicidade/etiologia , Propiofenonas/toxicidade , Propiofenonas/química , Receptor alfa de Estrogênio/metabolismo , Disruptores Endócrinos/toxicidade , Disruptores Endócrinos/química , Dano ao DNA/efeitos dos fármacosRESUMO
Membrane-based water treatment has emerged as a promising solution to address global water challenges. Graphene oxide (GO) has been successfully employed in membrane filtration processes owing to its reversible properties, large-scale production potential, layer-to-layer stacking, great oxygen-based functional groups, and unique physicochemical characteristics, including the creation of nano-channels. This review evaluates the separation performance of various GO-based membranes, manufactured by coating or interfacial polymerization with different support layers such as polymer, metal, and ceramic, for endocrine-disrupting compounds (EDCs) and pharmaceutically active compounds (PhACs). In most studies, the addition of GO significantly improved the removal efficiency, flux, porosity, hydrophilicity, stability, mechanical strength, and antifouling performance compared to pristine membranes. The key mechanisms involved in contaminant removal included size exclusion, electrostatic exclusion, and adsorption. These mechanisms could be ascribed to the physicochemical properties of compounds, such as molecular size and shape, hydrophilicity, and charge state. Therefore, understanding the removal mechanisms based on compound characteristics and appropriately adjusting the operational conditions are crucial keys to membrane separation. Future research directions should explore the characteristics of the combination of GO derivatives with various support layers, by tailoring diverse operating conditions and compounds for effective removal of EDCs and PhACs. This is expected to accelerate the development of surface modification strategies for enhanced contaminant removal.
Assuntos
Disruptores Endócrinos , Grafite , Membranas Artificiais , Poluentes Químicos da Água , Purificação da Água , Grafite/química , Disruptores Endócrinos/química , Purificação da Água/métodos , Poluentes Químicos da Água/química , Filtração , Adsorção , Água/químicaRESUMO
Bisphenol A (BPA), an endocrine disruptor, is widely used in food packaging materials, including drink containers. Sensitive detection of BPA is crucial to food safety. Herein, we have developed a novel optical-driven hydrogel film sensor for sensitive BPA detection based on the displacement of spiropyran (SP) from ß-cyclodextrin (ß-CD) cavity by BPA followed by the photochromism of the released SP. The released SP converts to the ring-opened merocyanine form which shows an enhanced red fluorescence in the dark. The sensor demonstrates a linear detection range from 0.1 to 20 µg mL-1 with a limit of detection at 0.027 µg mL-1 and a limit of quantification at 0.089 µg mL-1. Notably, the proposed ß-CD/SP hydrogel can be reused due to the reversible isomerization of SP and the reversible host-guest interaction. This sensor also shows good performance for BPA determination in real samples, indicating its great potential for food safety monitoring.
Assuntos
Compostos Benzidrílicos , Benzopiranos , Contaminação de Alimentos , Embalagem de Alimentos , Hidrogéis , Indóis , Nitrocompostos , Fenóis , beta-Ciclodextrinas , Fenóis/química , Fenóis/análise , beta-Ciclodextrinas/química , Hidrogéis/química , Compostos Benzidrílicos/química , Compostos Benzidrílicos/análise , Embalagem de Alimentos/instrumentação , Benzopiranos/química , Indóis/química , Nitrocompostos/química , Contaminação de Alimentos/análise , Limite de Detecção , Disruptores Endócrinos/análise , Disruptores Endócrinos/químicaRESUMO
Bisphenols and benzophenones are two typical kinds of endocrine-disrupting compounds (EDCs) that have been extensively detected in water environments, posing unanticipated risks to aquatic organisms and humans. It is urgent to develop efficient sample pretreatment methods for precise measurement of such EDCs. In this study, a magnetic and multi-shelled metal-organic framework derivative material has been prepared to extract and enrich trace bisphenols and benzophenones from water. Via a solvothermal reaction induced by sodium citrate followed by a carbonization treatment, a ZIF-67@ZIF-8 derived CoZn-magnetic hierarchical carbon (CoZn-MHC) material has been synthesized as a high-performance magnetic solid-phase extraction (MSPE) adsorbent. This adsorbent exhibited a good specific surface area (213.80 m2â g-1) and a saturation magnetization of 63.2 emu·g-1. After the optimization of several parameters (including adsorbent dosage, extraction time, pH, ionic strength, desorption solvent, and solvent volume), an efficient MSPE method for several EDCs (comprising bisphenols and benzophenones) was developed with a good linear range (R2 ≥ 0.990), a high sensitivity range (LODs: 0.793-5.37 ngâ L-1), and good reusability (RSD ≤4.67 % in five consecutive tests). Furthermore, the material exhibited commendable resistance to matrix interference in natural water samples with the recovery rates of target compounds ranging from 74.8 % to 107 %. We envision that the preparation strategy of this functional metal-organic framework (MOF)-based adsorbent for EDCs may provide insights for relevant research in the future.
Assuntos
Disruptores Endócrinos , Estruturas Metalorgânicas , Extração em Fase Sólida , Poluentes Químicos da Água , Extração em Fase Sólida/métodos , Disruptores Endócrinos/análise , Disruptores Endócrinos/isolamento & purificação , Disruptores Endócrinos/química , Estruturas Metalorgânicas/química , Adsorção , Poluentes Químicos da Água/isolamento & purificação , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/química , Fenóis/análise , Fenóis/isolamento & purificação , Fenóis/química , Benzofenonas/química , Benzofenonas/isolamento & purificaçãoRESUMO
The safety and health of aquatic organisms and humans are threatened by the increasing presence of pollutants in the environment. Endocrine disrupting chemicals are common pollutants which affect the function of endocrine and causes adverse effects on human health. These chemicals can disrupt metabolic processes by interacting with hormone receptors upon consumptions by humans or aquatic species. Several studies have reported the presence of endocrine disrupting chemicals in waterbodies, food, air and soil. These chemicals are associated with increasing occurrence of obesity, metabolic disorders, reproductive abnormalities, autism, cancer, epigenetic variation and cardiovascular risk. Conventional treatment processes are expensive, not environment friendly and unable to achieve complete removal of these harmful chemicals. In recent years, biochar from different sources has gained a considerable interest due to their adsorption efficiency with porous structure and large surface areas. biochar derived from lignocellulosic biomass are widely used as sustainable catalysts in soil remediation, carbon sequestration, removal of organic and inorganic pollutants and wastewater treatment. This review conceptualizes the production techniques of biochar from lignocellulosic biomass and explores the functionalization and interaction of biochar with endocrine-disrupting chemicals. This review also identifies the further needs of research. Overall, the environmental and health risks of endocrine-disrupting chemicals can be dealt with by biochar produced from lignocellulosic biomass as a sustainable and prominent approach.
Assuntos
Carvão Vegetal , Disruptores Endócrinos , Recuperação e Remediação Ambiental , Lignina , Carvão Vegetal/química , Disruptores Endócrinos/química , Disruptores Endócrinos/metabolismo , Lignina/química , Humanos , Recuperação e Remediação Ambiental/métodos , Adsorção , Poluentes Ambientais/química , Poluentes Ambientais/metabolismoRESUMO
Environmental contaminants, such as polycyclic aromatic hydrocarbons (PAHs), have raised concerns regarding their potential endocrine-disrupting effects on aquatic organisms, including fish. In this study, molecular docking and molecular dynamics techniques were employed to evaluate the endocrine-disrupting potential of PAHs in zebrafish, as a model organism. A virtual screening with 72 PAHs revealed a correlation between the number of PAH aromatic rings and their binding affinity to proteins involved in endocrine regulation. Furthermore, PAHs with the highest binding affinities for each protein were identified: cyclopenta[cd]pyrene for AR (-9.7 kcal/mol), benzo(g)chrysene for ERα (-11.5 kcal/mol), dibenzo(a,e)pyrene for SHBG (-8.7 kcal/mol), dibenz(a,h)anthracene for StAR (-11.2 kcal/mol), and 2,3-benzofluorene for TRα (-9.8 kcal/mol). Molecular dynamics simulations confirmed the stability of the protein-ligand complexes formed by the PAHs with the highest binding affinities throughout the simulations. Additionally, the effectiveness of the protocol used in this study was demonstrated by the receiver operating characteristic curve (ROC) analysis, which effectively distinguished decoys from true ligands. Therefore, this research provides valuable insights into the endocrine-disrupting potential of PAHs in fish, highlighting the importance of assessing their impact on aquatic ecosystems.
Assuntos
Disruptores Endócrinos , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Hidrocarbonetos Policíclicos Aromáticos , Peixe-Zebra , Hidrocarbonetos Policíclicos Aromáticos/química , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Animais , Disruptores Endócrinos/química , Disruptores Endócrinos/metabolismo , Disruptores Endócrinos/toxicidade , Ligação Proteica , Sítios de Ligação , Proteínas de Peixe-Zebra/metabolismo , Proteínas de Peixe-Zebra/química , Ligantes , Curva ROC , Poluentes Químicos da Água/metabolismo , Poluentes Químicos da Água/química , Poluentes Químicos da Água/toxicidade , Receptor alfa de Estrogênio/metabolismo , Receptor alfa de Estrogênio/químicaRESUMO
Pharmaceuticals, personal care products (PPCPs), and endocrine-disrupting compounds (EDCs) have seen a recent sustained increase in usage, leading to increasing discharge and accumulation in wastewater. Conventional water treatment and disinfection processes are somewhat limited in effectively addressing this micropollutant issue. Ultrasonication (US), which serves as an advanced oxidation process, is based on the principle of ultrasound irradiation, exposing water to high-frequency waves, inducing thermal decomposition of H2O while using the produced radicals to oxidize and break down dissolved contaminants. This review evaluates research over the past five years on US-based technologies for the effective degradation of EDCs and PPCPs in water and assesses various factors that can influence the removal rate: solution pH, temperature of water, presence of background common ions, natural organic matter, species that serve as promoters and scavengers, and variations in US conditions (e.g., frequency, power density, and reaction type). This review also discusses various types of carbon/non-carbon catalysts, O3 and ultraviolet processes that can further enhance the degradation efficiency of EDCs and PPCPs in combination with US processes. Furthermore, numerous types of EDCs and PPCPs and recent research trends for these organic contaminants are considered.
Assuntos
Cosméticos , Disruptores Endócrinos , Poluentes Químicos da Água , Purificação da Água , Disruptores Endócrinos/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/efeitos da radiação , Preparações Farmacêuticas/química , Cosméticos/química , Purificação da Água/métodos , Ultrassom , Ondas UltrassônicasRESUMO
Endocrine-disrupting chemicals (EDCs) are substances that can disrupt the normal functioning of hormones.Using aptamers, which are biological recognition elements, biosensors can quickly and accurately detect EDCs in environmental samples. However, the elucidation of aptamer structures by conventional methods is highly challenging due to their complexity. This has led to the development of three-dimensional aptamer structures based on different models and techniques. To do this, we developed a way to predict the 3D structures of the SS DNA needed for this sequence by starting with an aptamer sequence that has biosensor properties specific to bisphenol-A (BPA), one of the chemicals found in water samples that can interfere with hormones. In addition, we will elucidate the intermolecular mechanisms and binding affinity between aptamers and endocrine disruptors using bioinformatics techniques such as molecular docking, molecular dynamics simulation, and binding energies. The outcomes of our study are to compare modeling programs and force fields to see how reliable they are and how well they agree with results found in the existing literature, to understand the intermolecular mechanisms and affinity of aptamer-based biosensors, and to find a new way to make aptamers that takes less time and costs less.
Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Biologia Computacional , Disruptores Endócrinos , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Fenóis , Disruptores Endócrinos/química , Disruptores Endócrinos/análise , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Biologia Computacional/métodos , Fenóis/química , Fenóis/análise , Compostos Benzidrílicos/química , Compostos Benzidrílicos/análiseRESUMO
The endocrine system functions by interactions between ligands and receptors. Ligands exhibit potency for binding to and interacting with receptors. Potency is the product of affinity and efficacy. Potency and physiological concentration determine the ability of a ligand to produce physiological effects. The kinetic behavior of ligand-receptor interactions conforms to the laws of mass action. The laws of mass action define the relationship between the affinity of a ligand and the fraction of cognate receptors that it occupies at any physiological concentration. We previously identified the minimum ligand potency required to produce clinically observable estrogenic agonist effects via the human estrogen receptor-alpha (ERα). By examining data on botanical estrogens and dietary supplements, we demonstrated that ERα ligands with potency lower than one one-thousandth that of the primary endogenous hormone 17ß-estradiol (E2) do not produce clinically observable estrogenic effects. This allowed us to propose a Human-Relevant Potency Threshold (HRPT) for ERα ligands of 1 × 10-4 relative to E2. Here, we test the hypothesis that the HRPT for ERα arises from the receptor occupancy by the normal metabolic milieu of endogenous ERα ligands. The metabolic milieu comprises precursors to hormones, metabolites of hormones, and other normal products of metabolism. We have calculated fractional receptor occupancies for ERα ligands with potencies below and above the previously established HRPT when normal circulating levels of some endogenous ERα ligands and E2 were also present. Fractional receptor occupancy calculations showed that individual ERα ligands with potencies more than tenfold higher than the HRPT can compete for occupancy at ERα against individual components of the endogenous metabolic milieu and against mixtures of those components at concentrations found naturally in human blood. Ligands with potencies less than tenfold higher than the HRPT were unable to compete successfully for ERα. These results show that the HRPT for ERα agonism (10-4 relative to E2) proposed previously is quite conservative and should be considered strong evidence against the potential for disruption of the estrogenic pathway. For chemicals with potency 10-3 of E2, the potential for estrogenic endocrine disruption must be considered equivocal and subject to the presence of corroborative evidence. Most importantly, this work demonstrates that the endogenous metabolic milieu is responsible for the observed ERα agonist HRPT, that this HRPT applies also to ERα antagonists, and it provides a compelling mechanistic explanation for the HRPT that is grounded in basic principles of molecular kinetics using well characterized properties and concentrations of endogenous components of normal metabolism.
Assuntos
Disruptores Endócrinos , Estradiol , Receptor alfa de Estrogênio , Humanos , Disruptores Endócrinos/análise , Disruptores Endócrinos/química , Estradiol/metabolismo , Receptor alfa de Estrogênio/metabolismo , Receptor alfa de Estrogênio/agonistas , Estrogênios/metabolismo , LigantesRESUMO
Diclofenac (DCF) is an environmentally persistent, nonsteroidal anti-inflammatory drug (NSAID) with thyroid disrupting properties. Electrochemical advanced oxidation processes (eAOPs) can efficiently remove NSAIDs from wastewater. However, eAOPs can generate transformation products (TPs) with unknown chemical and biological characteristics. In this study, DCF was electrochemically degraded using a boron-doped diamond anode. Ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry was used to analyze the TPs of DCF and elucidate its potential degradation pathways. The biological impact of DCF and its TPs was evaluated using the Xenopus Eleutheroembryo Thyroid Assay, employing a transgenic amphibian model to assess thyroid axis activity. As DCF degradation progressed, in vivo thyroid activity transitioned from anti-thyroid in non-treated samples to pro-thyroid in intermediately treated samples, implying the emergence of thyroid-active TPs with distinct modes of action compared to DCF. Molecular docking analysis revealed that certain TPs bind to the thyroid receptor, potentially triggering thyroid hormone-like responses. Moreover, acute toxicity occurred in intermediately degraded samples, indicating the generation of TPs exhibiting higher toxicity than DCF. Both acute toxicity and thyroid effects were mitigated with a prolonged degradation time. This study highlights the importance of integrating in vivo bioassays in the environmental risk assessment of novel degradation processes.