RESUMO
The latex of Ipomoea (Convolvulaceae) is a source of a special kind of acylsugars called resin glycosides, which are highly appreciated because of their biological activities (i.e. laxative, antimicrobial, cytotoxic etc.). Most research has been conducted in perennials with tuberous roots, where resin glycosides are stored. However, their content and variation are unknown in annual vines that lack this type of root, such as in the case of Ipomoea parasitica. This species contains research/biological and human value through its fast growth, survival in harsh environments, and employment in humans for mental/cognitive improvements. These qualities make I. parasitica an ideal system to profile resin glycosides and their variations in response to edaphoclimate. Topsoil samples (0-30 cm depth) and latex from petioles of I. parasitica were collected in two localities of central Mexico. The latex was analyzed through UHPLC-ESI-QTOF, and soil physico-chemical characteristics, the rainfall, minimum, average, and maximum temperatures were recorded. We also measured canopy (%), rockiness (%), and plant cover (%). A Principal Component Analysis was conducted to find associations between edaphoclimate and the resin glycosides. Forty-four resin glycosides were found in the latex of I. parasitica. Ten correlated significantly with three components (47.07%) and contained tetrasaccharide, pentasaccharide, and dimers of tetrasaccharide units. Five resin glycosides were considered constitutive because they were in all the plants. However, exclusive molecules to each locality were also present, which we hypothesize is in response to significant microhabitat conditions found in this study (temperature, clay content, pH, and potassium). Our results showed the presence of resin glycosides in I. parasitica latex and are the basis for experimentally testing the effect of the conditions above on these molecules. However, ecological, molecular, and biochemical factors should be considered in experiments designed to produce these complex molecules.
Assuntos
Glicosídeos , Ipomoea , Resinas Vegetais , Glicosídeos/química , Ipomoea/química , Resinas Vegetais/química , México , Látex/química , Solo/químicaRESUMO
The physico-chemical and biological properties of natural rubber latex (NRL), entailing its biodegradability and biocompatibility, render it a promising material for various biomedical applications. This research explores the facile blending of NRL with dextrin in different compositions to investigate its potential as a prospective UV shielding transdermal patch for biomedical applications. The superior compatibility between the polymers after blending and the improved thermal stability have been established through FTIR, DSC, and TGA examinations, respectively. Optimization of blended polymers for compatibility, wettability, crystallinity, and static mechanical properties has been performed. Morphology characterization conducted via SEM and AFM techniques suggests a uniform morphology for the optimized blend system. The UV shielding ability of the blend has been confirmed by the evaluation of in-vitro UV shielding performance, UV protection factor (UPF), and the superior protection of the optimized system on living cells upon UV irradiation. The observed cell viability, swelling, erosion, porosity, hemocompatibility, and soil degradation properties suggest the NRL-DXT combination for the possible development of high-quality transdermal patches.
Assuntos
Materiais Biocompatíveis , Dextrinas , Látex , Borracha , Adesivo Transdérmico , Raios Ultravioleta , Dextrinas/química , Materiais Biocompatíveis/química , Borracha/química , Látex/química , Humanos , Sobrevivência Celular/efeitos dos fármacosRESUMO
Background: Plants allocate resources to growth, defense, and stress resistance, and resource availability can affect the balance between these allocations. Allocation patterns are well-known to differ among species, but what controls possible intra-specific trade-offs and if variation in growth vs. defense potentially evolves in adaptation to resource availability. Methods: We measured growth and defense in a provenance trial of rubber trees (Hevea brasiliensis) with clones originating from the Amazon basin. To test hypotheses on the allocation to growth vs. defense, we relate biomass growth and latex production to wood and leaf traits, to climate and soil variables from the location of origin, and to the genetic relatedness of the Hevea clones. Results: Contrary to expectations, there was no trade-off between growth and defense, but latex yield and biomass growth were positively correlated, and both increased with tree size. The absence of a trade-off may be attributed to the high resource availability in a plantation, allowing trees to allocate resources to both growth and defense. Growth was weakly correlated with leaf traits, such as leaf mass per area, intrinsic water use efficiency, and leaf nitrogen content, but the relative investment in growth vs. defense was not associated with specific traits or environmental variables. Wood and leaf traits showed clinal correlations to the rainfall and soil variables of the places of origin. These traits exhibited strong phylogenetic signals, highlighting the role of genetic factors in trait variation and adaptation. The study provides insights into the interplay between resource allocation, environmental adaptations, and genetic factors in trees. However, the underlying drivers for the high variation of latex production in one of the commercially most important tree species remains unexplained.
Assuntos
Hevea , Látex , Folhas de Planta , Hevea/genética , Hevea/crescimento & desenvolvimento , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Látex/metabolismo , Biomassa , Madeira/genética , Filogenia , Especificidade da EspécieRESUMO
Objective: To investigate the biological safety of commercially available natural rubber latex and synthetic polyurethane condoms. Methods: Natural rubber latex condom brands of A1 and A2 and polyurethane condom brands of B1 and B2 were purchased from large chain pharmacies in Chengdu, with three packages randomly selected for each brand. The study assessed the toxic effects of condom extracts on L-929 mouse fibroblasts according to GB/T standards. Gross observation and histopathological evaluation were conducted to assess the irritation reactions of condoms on the vagina and penis of rabbits (3 rabbits were used for each brand), as well as their sensitization effects on guinea pig skin. Additionally, the impact of continuous perfusion of condom extracts of the vaginas of SD rats for 30 days on their reproductive systems was evaluated, following GB/T standards (5 rats were used for each brand). Results: Extracts from natural rubber latex condom brands A1 and A2, at concentrations of 100% and 50%, exhibited significant cytotoxicity, with optical density (OD) values being significantly lower than those of the blank control group and the polyurethane condom brands B1 and B2 (P<0.01). There was no significant difference in cell morphology and OD values between the extracts of B1 and B2 and the blank control group (P>0.05). Vaginal congestion was found in 3 rabbits from A1 group and 1 rabbit from the A2 group, while no obvious congestion was noted in rabbits from the B1 and the B2 groups. Histopathological examination showed scattered inflammatory cell infiltration in the vaginal tissue of 3 rabbits from the A1 group and 2 rabbits from the A2 group, and slight congestion in the blood vessels of the lamina propria. No obvious pathological changes were observed in the vaginal tissue of polyurethane brand rabbits. Two rabbits from the A1 group and 1 rabbit from the A2 group showed transient and mild erythema on the penis during the experiment. Histopathological examination showed that 1 rabbit from A1 group had small foci of pericapillary lymphocytes in the dermis of the penis, while no significant pathological changes were observed in the penile tissue of A2, B1, and B2 groups. After 30 days of continuous vaginal perfusion with condom extract, 3 rats in A1 group and 2 rats in the A2 group had uterine congestion, with the degree of congestion being lower in the A2 group. No significant congestion or pathological changes were observed in the vaginal and penile tissues of rabbits, or in the uterine tissues of rats from the polyurethane groups. None of the 4 groups of guinea pigs showed significant skin allergic reactions to the condom extracts. Conclusion: Significant differences in biosafety exist among condoms of various materials and brands. To ensure product safety, it is crucial to strengthen quality control and regulatory oversight after condoms become commercially available.
Assuntos
Preservativos , Látex , Poliuretanos , Ratos Sprague-Dawley , Animais , Coelhos , Feminino , Cobaias , Ratos , Masculino , Látex/química , Camundongos , Vagina/patologia , Fibroblastos/efeitos dos fármacos , Teste de Materiais , Borracha/efeitos adversosRESUMO
The spurge Euphorbia characias is known for its latex, which is rich in antioxidant enzymes and anti-phytopathogen molecules. In this study, we identified a novel polyubiquitin protein in the latex and leaves, leading to the first molecular characterization of its coding gene and expressed protein in E. characias. Using consensus-degenerate hybrid oligonucleotide primers (CODEHOP) and rapid amplification of cDNA ends (5'/3'-RACE), we reconstructed the entire open reading frame (ORF) and noncoding regions. Our analysis revealed that the polyubiquitin gene encodes five tandemly repeated sequences, each coding for a ubiquitin monomer with amino acid variations in four of the five repeats. In silico studies have suggested functional differences among monomers. Gene expression peaked during the summer, correlating with high temperatures and suggesting a role in heat stress response. Western blotting confirmed the presence of polyubiquitin in the latex and leaf tissues, indicating active ubiquitination processes. These findings enhance our understanding of polyubiquitin's regulatory mechanisms and functions in E. characias, highlighting its unique structural and functional features.
Assuntos
Euphorbia , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas , Poliubiquitina , Euphorbia/genética , Poliubiquitina/genética , Poliubiquitina/metabolismo , Proteínas de Plantas/genética , Estações do Ano , Látex/metabolismo , Látex/química , Folhas de Planta/genética , Folhas de Planta/metabolismo , FilogeniaRESUMO
Solar steam generation (SSG) offers a sustainable approach to fresh water production. Herein, a novel dual-functional natural rubber/carbon black composite foam evaporator is presented for a cost-efficient SSG system that both produces fresh water and eliminates heavy metals present in the water. The composite foam is produced using the Dunlop process, and in its optimized form, it absorbed >96 % of sunlight. The foam evaporator exhibited a thermal conductivity of 0.052 W/mâ K, a water evaporation rate of 1.40 kg/m2/h, converted 83.38 % of light to heat under 1 sun irradiation, and showed outstanding stability. The technology required to produce this composite foam is already available to make large-scale production feasible, while the natural raw materials are abundant. On the basis of its performance qualities, the rubber foam composite appears to be an excellent candidate for application as a viable solar absorber for SSG to produce fresh, clean water for commercial purposes.
Assuntos
Metais Pesados , Borracha , Luz Solar , Borracha/química , Metais Pesados/química , Látex/química , Purificação da Água/métodos , Água/química , Descontaminação/métodos , Vapor , Poluentes Químicos da Água/química , Fuligem/químicaRESUMO
Antimicrobial surfaces limit the spread of infectious diseases. To date, there is no antimicrobial coating that has widespread use because of short-lived and limited spectrum efficacy, poor resistance to organic material, and/or cost. Here, we present a paint based on waterborne latex particles that is supramolecularly associated with quaternary ammonium compounds (QACs). The optimal supramolecular pairing was first determined by immobilizing selected ions on self-assembled monolayers exposing different groups. The QAC surface loading density was then increased by using polymer brushes. These concepts were adopted to develop inexpensive paints to be applied on many different surfaces. The paint could be employed for healthcare and food production applications. Its slow release of QAC allows for long-lasting antimicrobial action, even in the presence of organic material. Its efficacy lasts for more than 90 washes, and importantly, once lost, it can readily be restored by spraying an aqueous solution of the QAC. We mainly tested cetyltrimethylammonium as QAC as it is already used in consumer care products. Our antimicrobial paint is broad spectrum as it showed excellent antimicrobial efficiency against four bacteria and four viruses.
Assuntos
Compostos de Amônio Quaternário , Compostos de Amônio Quaternário/química , Compostos de Amônio Quaternário/farmacologia , Anti-Infecciosos/farmacologia , Anti-Infecciosos/química , Pintura , Propriedades de Superfície , Látex/química , Látex/farmacologia , Testes de Sensibilidade Microbiana , Bactérias/efeitos dos fármacosRESUMO
Food allergies mediated by specific IgE (sIgE) have a significant socioeconomic impact on society. Evaluating the IgE cross-reactivity between allergens from different allergen sources can enable the better management of these potentially life-threatening adverse reactions to food proteins and enhance food safety. A novel banana fruit allergen, S-adenosyl-L-homocysteine hydrolase (SAHH), has been recently identified and its recombinant homolog was heterologously overproduced in E. coli. In this study, we performed a search in the NCBI (National Center for Biotechnology Information) for SAHH homologs in ryegrass, latex, and kiwifruit, all of which are commonly associated with pollen-latex-fruit syndrome. In addition, Western immunoblot analysis was utilized to identify the cross-reactive IgE to banana SAHH in the sera of patients with a latex allergy, kiwifruit allergy, and ryegrass allergy. ClustalOmega analysis showed more than 92% amino acid sequence identity among the banana SAHH homologs in ryegrass, latex, and kiwifruit. In addition to five B-cell epitopes, in silico analysis predicted eleven T-cell epitopes in banana SAHH, seventeen in kiwifruit SAHH, twelve in ryegrass SAHH, and eight in latex SAHH, which were related to the seven-allele HLA reference set (HLA-DRB1*03:01, HLA-DRB1*07:01, HLA-DRB1*15:01, HLA-DRB3*01:01, HLA-DRB3*02:02, HLA-DRB4*01:01, HLA-DRB5*01:01). Four T-cell epitopes were identical in banana and kiwifruit SAHH (positions 328, 278, 142, 341), as well as banana and ryegrass SAHH (positions 278, 142, 96, and 341). All four SAHHs shared two T-cell epitopes (positions 278 and 341). In line with the high amino acid sequence identity and B-cell epitope homology among the analyzed proteins, the cross-reactive IgE to banana SAHH was detected in three of three latex-allergic patients, five of six ryegrass-allergic patients, and two of three kiwifruit-allergic patients. Although banana SAHH has only been studied in a small group of allergic individuals, it is a novel cross-reactive food allergen that should be considered when testing for pollen-latex-fruit syndrome.
Assuntos
Actinidia , Alérgenos , Reações Cruzadas , Hipersensibilidade Alimentar , Imunoglobulina E , Látex , Musa , Humanos , Reações Cruzadas/imunologia , Hipersensibilidade Alimentar/imunologia , Alérgenos/imunologia , Alérgenos/genética , Musa/imunologia , Musa/genética , Imunoglobulina E/imunologia , Actinidia/imunologia , Feminino , Látex/imunologia , Masculino , Proteínas de Plantas/imunologia , Proteínas de Plantas/genética , Adulto , Antígenos de Plantas/imunologia , Antígenos de Plantas/genética , Sequência de Aminoácidos , Epitopos de Linfócito T/imunologia , Pessoa de Meia-Idade , Adolescente , Criança , Adulto JovemRESUMO
Paper mulberry (Broussonetia papyrifera) is a fast-growing tree known for its tolerance to diverse biotic and abiotic stresses. To explore genes combating Verticillium wilt, a devasting and formidable disease damage to cotton and many economically significant crops, we purified an antifungal protein, named BpAFP, from the latex of paper mulberry. Based on peptide fingerprint, we cloned the full cDNA sequence of BpAFP and revealed that BpAFP belongs to Class I chitinases, sharing 74â¯% identity with B. papyrifera leaf chitinase, PMAPII. We further introduced BpAFP into Arabidopsis, tobacco, and cotton. Transgenic plants exhibited significant resistance to Verticillium wilt. Importantly, BpAFP also demonstrated insecticidal activity against herbivorous pests, Plutella xylostella, and Prodenia litura, when feeding the larvae with transgenic leaves. Our finding unveils a dual role of BpAFP in conferring resistance to both plant diseases and lepidopterous pests.
Assuntos
Quitinases , Látex , Mariposas , Doenças das Plantas , Plantas Geneticamente Modificadas , Verticillium , Doenças das Plantas/microbiologia , Doenças das Plantas/parasitologia , Quitinases/metabolismo , Quitinases/genética , Animais , Mariposas/fisiologia , Verticillium/fisiologia , Látex/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Resistência à Doença/genética , Filogenia , Arabidopsis/genética , Arabidopsis/microbiologiaRESUMO
Latex clearing proteins (Lcps) catalyze the oxidative cleavage of the C = C bonds in cis-1,4-polyisoprene (natural rubber), producing oligomeric compounds that can be repurposed to other materials. The active catalytic site of Lcps is buried inside the protein structure, thus raising the question of how the large hydrophobic rubber chains can access the catalytic center. To improve our understanding of hydrophobic polymeric substrate binding to Lcps and subsequent catalysis, we investigated the interaction of a substrate model containing ten carbon-carbon double bonds with the structurally characterized LcpK30, using multiple computational tools. Prediction of the putative tunnels and cavities in the LcpK30 structure, using CAVER-Pymol plugin 3.0.3, fpocket and Molecular Dynamic (MD) simulations provided valuable insights on how substrate enters from the surface to the buried active site. Two dominant tunnels were discovered that provided feasible routes for substrate binding, and the presence of two hydrophobic pockets was predicted near the heme cofactor. The larger of these pockets is likely to accommodate the substrate and to determine the size distribution of the oligomers. Protein-ligand docking was carried out using GOLD software to predict the conformations and interactions of the substrate within the protein active site. Deeper insight into the protein-substrate interactions, including close-contacts, binding energies and potential cleavage sites in the cis-1,4-polyisoprene, were obtained from MD simulations. Our findings provide further justification that the protein-substrate complexation in LcpK30 is mainly driven by the hydrophobic interactions accompanied by mutual conformational changes of both molecules. Two potential binding modes were identified, with the substrate in either extended or folded conformations. Whilst binding in the extended conformation was most favorable, the folded conformation suggested a preference for cleavage of a central double bond, leading to a preference for oligomers with 5 to 6 C = C bonds. The results provide insight into further enzyme engineering studies to improve catalytic activity and diversify the substrate and product scope of Lcps.
Assuntos
Hemiterpenos , Látex , Simulação de Dinâmica Molecular , Ligação Proteica , Hemiterpenos/metabolismo , Hemiterpenos/química , Látex/química , Látex/metabolismo , Simulação de Acoplamento Molecular , Proteínas de Plantas/metabolismo , Proteínas de Plantas/química , Domínio Catalítico , Interações Hidrofóbicas e Hidrofílicas , Sítios de Ligação , Butadienos/química , Butadienos/metabolismoRESUMO
In Brazil, latex from Euphorbia umbellata (African milk tree) has been increasingly used in folk medicine to treat several types of cancer, including melanoma. The effect of lyophilized latex (LL), its hydroethanolic extract (E80), triterpene (F-TRI)- and diterpene (F-DIT)-enriched fractions, along with six isolated phorbol esters from LL and phorbol 12-myristate 13-acetate (PMA) on J774A.1, THP-1, SK-MEL-28, and B16-F10 cell line viability were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method. The compounds were identified by 2D-NMR and HRESIMS. The effect of the LL, extract and fractions on cell viability was also assessed through a resazurin reduction assay. At 100 µg/ml, LL, and its fractions moderately inhibited J774A.1 (37.5-59.5%) and THP-1 (12.6-43.6%) metabolism. LL (IC50 70 µg/ml) and F-TRI (IC50 68 µg/ml) were barely more effective against B16-F10 cells, and only F-TRI exerted an inhibitory effect on SK-MEL-28 cells (IC50 66-75 µg/ml). The samples did not effectively inhibit THP-1 growth (IC50 69-87 µg/ml, assessed by MTT). B16-F10 was susceptible to PMA (IC50 53 µM) and two 12-phenylacetate esters (IC50 56-60 µM), while SK-MEL-28 growth was inhibited (IC50 58 µM) by one of these kinds of esters with an additional 4ß-deoxy structure. Synagrantol A (IC50 39 µM) was as effective as PMA (IC50 47 µM) in inhibiting J774A.1 growth in a dose-dependent manner. Furthermore, an in silico study with target receptors indicated a high interaction of the compounds with the PKC proteins. These results provide useful knowledge on the effect of tigliane-type diterpenes on tumor cell from the perspective of medicinal chemistry.
Assuntos
Euphorbia , Látex , Ésteres de Forbol , Euphorbia/química , Látex/química , Ésteres de Forbol/farmacologia , Humanos , Camundongos , Animais , Linhagem Celular Tumoral , Estrutura Molecular , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Brasil , Monócitos/efeitos dos fármacos , Compostos Fitoquímicos/farmacologia , Compostos Fitoquímicos/isolamento & purificação , Sobrevivência Celular/efeitos dos fármacos , Diterpenos/farmacologia , Diterpenos/isolamento & purificação , Terpenos/farmacologia , Terpenos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Antineoplásicos Fitogênicos/isolamento & purificação , Acetato de Tetradecanoilforbol , Melanoma/tratamento farmacológicoRESUMO
Cancer is a fatal disease, and unfortunately, the anticancer drugs harm normal cells. Plant's extracts are the golden key to solving this issue. In this research, fig latex - from Ficus carica- was encapsulated using cellulose acetate (CA) and poly (ethylene oxide) (PEO) polymers via electrospinning method (Fig@CA/PEO). Fig@CA/PEO nanofiber scaffold was characterized by thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). The average fiber diameter was decreased with an increase in latex concentration from 715 nm to 583 nm. FT-IR spectroscopy indicated the presence of fig latex in Fig@CA/PEO nanofibers. Compared to 5-fluorouracil, Fig@CA/PEO nanofiber scaffold considered safe towards normal cells (WI-38). Moreover, the nanofiber scaffold was efficient against colon cancer cells (Caco) and liver cancer cells (HepG2) as it demonstrated IC50 values for cells by 23.97 µg/mL and 23.96 µg/mL, respectively. Besides, the nanofiber scaffold revealed mechanistic variations in apoptotic oncogenes; described by the upregulation of BCL2 and P21, combined by downregulation of p53 and TNF. Moreover, the nanofiber scaffold showed antioxidant activity counting 33.4, 36 and 41 % of DPPH scavenging as the fig latex concentration increased. The results demonstrate that the Fig@CA/PEO nanofiber scaffold is a promising substitute to traditional chemotherapy.
Assuntos
Antineoplásicos , Antioxidantes , Celulose , Ficus , Látex , Nanofibras , Polietilenoglicóis , Nanofibras/química , Celulose/química , Celulose/análogos & derivados , Celulose/farmacologia , Humanos , Ficus/química , Polietilenoglicóis/química , Antioxidantes/farmacologia , Antioxidantes/química , Látex/química , Látex/farmacologia , Antineoplásicos/farmacologia , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Células Hep G2 , Espectroscopia de Infravermelho com Transformada de Fourier , Linhagem Celular TumoralRESUMO
Sustainability and circularity are key issues facing the global polymer industry. The search for biodegradable and environmentally-friendly polymers that can replace conventional materials is a difficult challenge that has been met with limited success. Alternatives must be cost-effective, scalable, and provide equivalent performance. We report that latexes made by the conventional emulsion polymerization of vinyl acetate and functional vinyl ester monomers are efficient thickeners for consumer products and biodegrade in wastewater. This approach uses readily-available starting materials and polymerization is carried out in water at room temperature, in one pot, and generates negligible waste. Moreover, the knowledge that poly(vinyl ester)s are biodegradable will lead to the design of new green polymer materials.
Assuntos
Emulsões , Emulsões/química , Polimerização , Polímeros/química , Álcalis/química , Biodegradação Ambiental , Látex/química , Compostos de Vinila/química , Águas Residuárias/químicaRESUMO
Laticifers have been utilized as paradigms to enhance comprehension of specific facets of plant ecology and evolution. From the beginning of seedling growth, autonomous laticifer networks are formed throughout the plant structure, extending across all tissues and organs. The vast majority of identified products resulting from laticifer chemistry and metabolism are linked to plant defense. The latex, which is the fluid contained within laticifers, is maintained under pressure and has evolved to serve as a defense mechanism against both aggressors and invaders, irrespective of their capabilities or tactics. Remarkably, the latex composition varies among different species. The current goal is to understand the specific functions of various latex components in combating plant enemies. Therefore, the study of latex's chemical composition and proteome plays a critical role in advancing our understanding about plant defense mechanisms. Here, we will discuss some of these aspects.
Assuntos
Látex , Látex/metabolismo , Defesa das Plantas contra Herbivoria , Plantas/metabolismoRESUMO
As a result of increasing drug resistance, crossover resistance development, prolonged therapy, and the absence of different agents with innovative methods for implementation, the efficacy of recent antileishmanial medications is severely declining. So, it is vital to look for other medications from botanical remedies that have antileishmanial activity. The latex of Euphorbia abyssinica (E abyssinica) and the leaves of Clematis simensis fresen (C simensis) were macerated in methanol (80%). In vitro antileishmanial activity of the preparation was tried on promastigotes of Leishmania aethiopica (L aethiopica) and Leishmania donovani (L donovani) using resazurin assay, and fluorescence intensity was measured. One percent of dimethyl sulfoxide (DMSO) and media as negative control and amphotericin B as positive control were used. Additionally, hemolytic & phytochemical tests of the preparation were done. The mean and standard errors of each extract were evaluated and interpreted for statistical significance using one-way analysis of variance. From sigmoidal dose-response curves of % inhibition, half maximal inhibitory concentration (IC50) values were determined by GraphPad Prism and Microsoft Excel; outcomes were presented as meanâ ±â standard error of mean of triplicate trials. Pâ <â .05 was statistical significance. The phytochemical screening of C simensis and E abyssinica confirmed the existence of steroids, phenols, tannins, saponins, alkaloids, terpenoids, flavonoids and glycosides. C simensis possesses antileishmanial activity with IC50 outcomes of 46.12â ±â 0.03 and 8.18â ±â 0.10 µg/mL on the promastigotes of L aethiopica and L donovani, respectively. However, E abyssinica showed stronger activity with IC50 outcomes of 16.07â ±â 0.05 µg/mL and 4.82â ±â 0.07 µg/mL on L aethiopica and L donovani, respectively. C simensis and E abyssinica have a less hemolytic effect on human red blood cells at low concentrations. The outcomes from this investigation demonstrated that the preparation of C simensis and E abyssinica indicated significant antileishmanial activity. Therefore, further in vivo assessment of antileishmanial, cytotoxicity activity and quantitative identification of secondary metabolites are highly recommended.
Assuntos
Antiprotozoários , Euphorbia , Látex , Extratos Vegetais , Folhas de Planta , Extratos Vegetais/farmacologia , Euphorbia/química , Látex/farmacologia , Látex/química , Antiprotozoários/farmacologia , Folhas de Planta/química , Humanos , Leishmania donovani/efeitos dos fármacos , Concentração Inibidora 50 , Leishmania/efeitos dos fármacos , Metanol , Solventes , Hemólise/efeitos dos fármacosRESUMO
Natural Rubber Latex (NRL) has shown to be a promising biomaterial for use as a drug delivery system to release various bioactive compounds. It is cost-effective, easy to handle, biocompatible, and exhibits pro-angiogenic and pro-healing properties for both soft and hard tissues. NRL releases compounds following burst and sustained release kinetics, exhibiting first-order release kinetics. Moreover, its pore density can be adjusted for tailored kinetics profiles. In addition, biotechnological applications of NRL in amblyopia, smart mattresses, and neovaginoplasty have demonstrated success. This comprehensive review explores NRL's diverse applications in biotechnology and biomedicine, addressing challenges in translating research into clinical practice. Organized into eight sections, the review emphasizes NRL's potential in wound healing, drug delivery, and metallic nanoparticle synthesis. It also addresses the challenges in enhancing NRL's physical properties and discusses its interactions with the human immune system. Furthermore, examines NRL's potential in creating wearable medical devices and biosensors for neurological disorders. To fully explore NRL's potential in addressing important medical conditions, we emphasize throughout this review the importance of interdisciplinary research and collaboration. In conclusion, this review advances our understanding of NRL's role in biomedical and biotechnological applications, offering insights into its diverse applications and promising opportunities for future development.
Assuntos
Materiais Biocompatíveis , Sistemas de Liberação de Medicamentos , Látex , Medicina Regenerativa , Borracha , Humanos , Materiais Biocompatíveis/química , Látex/química , Medicina Regenerativa/métodos , Borracha/química , Cicatrização/efeitos dos fármacosRESUMO
Gunshot wound analysis is an important part of medicolegal practice, in both autopsies and examinations of living persons. Well-established and studied simulants exist that exhibit both physical and biomechanical properties of soft-tissues and bones. Current research literature on ballistic wounds focuses on the biomechanical properties of skin simulants. In our extensive experimental study, we tested numerous synthetic and natural materials, regarding their macromorphological bullet impact characteristics, and compared these data with those from real bullet injuries gathered from medicolegal practice. Over thirty varieties of potential skin simulants were shot perpendicularly, and at 45°, at a distance of 10 m and 0.3 m, using full metal jacket (FMJ) projectiles (9 × 19 mm Luger). Simulants included ballistic gelatine at various concentrations, dental silicones with several degrees of hardness, alginates, latex, chamois leather, suture trainers for medical training purposes and various material compound models. In addition to complying to the general requirements for a synthetic simulant, results obtained from dental silicones shore hardness 70 (backed with 20 % by mass gelatine), were especially highly comparable to gunshot entry wounds in skin from real cases. Based on these results, particularly focusing on the macroscopically detectable criteria, we can strongly recommend dental silicone shore hardness 70 as a skin simulant for wound ballistics examinations.
Assuntos
Balística Forense , Gelatina , Pele , Ferimentos por Arma de Fogo , Ferimentos por Arma de Fogo/patologia , Balística Forense/métodos , Humanos , Pele/lesões , Pele/patologia , Látex , Silicones , Modelos Biológicos , DurezaRESUMO
The latex of Euphorbia peplus and its major component 20-deoxyingenol-3-angelate (DI3A) displayed significant nematicidal activity against Caenorhabditis elegans and Panagrellus redivivus. DI3A treatment inhibited the growth and development of nematodes and caused significantly negative effects on locomotion behavior, reproduction, and accumulation of reactive oxygen species. Transcriptome analysis indicated that differential expression genes in DI3A-treated C. elegans were mainly associated with the metabolism, growth, and development process, which were further confirmed by RT-qPCR experiments. The expression level of TPA-1 gene encoding a protein kinase C isotype was obviously upregulated by DI3A treatment, and knockdown of TPA-1 by RNAi technology in the nematode could relieve the growth-inhibitory effect of DI3A. Metabolic analysis indicated that DI3A was hardly metabolized by C. elegans, but a glycosylated indole derivative was specifically accumulated likely due to the activation of detoxification. Overall, our findings suggested that DI3A from E. peplus latex exerted a potent nematicidal effect through the gene TPA-1, which provides a potential target for the control of nematodes and also suggests the potential application value of E. peplus latex and DI3A as botanical nematicides.
Assuntos
Antinematódeos , Caenorhabditis elegans , Euphorbia , Látex , Proteína Quinase C , Animais , Caenorhabditis elegans/efeitos dos fármacos , Caenorhabditis elegans/genética , Caenorhabditis elegans/enzimologia , Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/crescimento & desenvolvimento , Látex/química , Látex/metabolismo , Antinematódeos/farmacologia , Antinematódeos/química , Antinematódeos/metabolismo , Euphorbia/química , Proteína Quinase C/metabolismo , Proteína Quinase C/genética , Extratos Vegetais/farmacologia , Extratos Vegetais/químicaRESUMO
Different cellulose nanocrystal (CNC) forms (dried vs never-dried) can lead to different degrees of CNC reassembly, the formation of nanofibril-like structures, in nanocomposite latex-based pressure-sensitive adhesive (PSA) formulations. CNC reassembly is also affected by CNC sonication and loading as well as the protocol used for CNC addition to the polymerization. In this study, carboxylated CNCs (cCNCs) were incorporated into a seeded, semibatch, 2-ethylhexyl acrylate/methyl methacrylate/styrene emulsion polymerization and cast as pressure-sensitive adhesive (PSA) films. The addition of CNCs led to a simultaneous increase in tack strength, peel strength, and shear adhesion, avoiding the typical trade-off between the adhesive and cohesive strength. Increased CNC reassembly resulted from the use of dried, redispersed, and sonicated cCNCs, along with increased cCNC loading and addition of the cCNCs at the seed stage of the polymerization. The increased degree of CNC reassembly was shown to significantly increase the shear adhesion by enhancing the elastic modulus of the PSA films.
Assuntos
Adesivos , Celulose , Látex , Nanopartículas , Celulose/química , Adesivos/química , Nanopartículas/química , Látex/química , Polimerização , Nanocompostos/química , PressãoRESUMO
Waterborne coatings with intrinsic antibacterial attributes have attracted significant attention due to their potential in mitigating microbial contamination while simultaneously addressing the environmental drawbacks of their solvent-based counterparts. Typically, antimicrobial coatings are designed to resist and eliminate microbial threats, encompassing challenges such as biofilm formation, fungal contamination, and proliferation of black mold. Iodine, when solubilized using ethylene glycol and incorporated as a complex into waterborne latex dispersions, has shown remarkable antimicrobial activity. Here, we demonstrate the effect of the film formation process of these iodinated latex dispersions on their antimicrobial properties. The effect of iodine on the surface morphology and mechanical, adhesion, and antimicrobial properties of the generated films was investigated. Complete integration and uniform distribution of iodine in the films were confirmed through UV-vis spectrophotometry and a laser Raman imaging system (LRIS). In terms of properties, iodinated films showed improved mechanical strength and adhesion compared with blank films. Further, the presence of iodine rendered the films rougher, making them susceptible to bacterial adhesion, but interestingly provided enhanced antibiofilm activity. Moreover, thicker films had a lower surface roughness and reduced biofilm growth. These observations are elucidated through the complex interplay among film thickness, surface morphology, and iodine properties. The insights into the interlink between the film formation process and antimicrobial properties of iodinated latex dispersions will facilitate their enhanced application as sustainable alternatives to solvent-based coatings.