RESUMO
Infectious pancreatic necrosis virus (IPNV) causes economic losses with a highly variable mortality rate worldwide, especially in rainbow trout. The virus has a double-stranded bi-partite RNA genome designated segment A and B. New complete genome sequences of nine rainbow trout isolates from Turkey were determined and subjected to phylogenetic analysis, identifying all as genotype 5 (serotype Sp). A time-dependent change in the extended pathogenicity motif of VP2 from P217T221A247 (PTA) to PTE P217T221E247 over a period of 10 years was identified. A wider analysis of 99 IPNV sequences from Turkey and Iran revealed the emergence of the motif PTE from 2007 to 2017, inducing significant morbidity in fry by 2013. In fact, displacement of the PTA motif, by the PTE motif in IPNV isolates appeared to be connected to a production peak of rainbow trout in 2013. An additional CAI analysis provided more evidence, indicating that rainbow trout culture in Turkey has an influence on the evolution of IPNV.
Assuntos
Infecções por Birnaviridae , Doenças dos Peixes , Vírus da Necrose Pancreática Infecciosa , Oncorhynchus mykiss , Animais , Motivos de Aminoácidos , Aquicultura , Infecções por Birnaviridae/veterinária , Infecções por Birnaviridae/virologia , Evolução Molecular , Doenças dos Peixes/virologia , Genoma Viral , Genótipo , Vírus da Necrose Pancreática Infecciosa/genética , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Vírus da Necrose Pancreática Infecciosa/isolamento & purificação , Vírus da Necrose Pancreática Infecciosa/classificação , Oncorhynchus mykiss/virologia , Filogenia , Turquia , Proteínas Estruturais Virais/genética , VirulênciaRESUMO
In salmon farming, viruses are responsible for outbreaks that produce significant economic losses for which there is a lack of control tools other than vaccines. Type I interferon has been successfully used for treating some chronic viral infections in humans. However, its application in salmonids depends on the proper design of a vehicle that allows its massive administration, ideally orally. In mammals, administration of recombinant probiotics capable of expressing cytokines has shown local and systemic therapeutic effects. In this work, we evaluate the use of Lactococcus lactis as a type I Interferon expression system in Atlantic salmon, and we analyze its ability to stimulate the antiviral immune response against IPNV, in vivo and in vitro. The interferon expressed in L. lactis, even though it was located mainly in the bacterial cytoplasm, was functional, stimulating Mx and PKR expression in CHSE-214 cells, and reducing the IPNV viral load in SHK-1 cells. In vivo, the oral administration of this L. lactis producer of Interferon I increases Mx and PKR expression, mainly in the spleen, and to a lesser extent, in the head kidney. The oral administration of this strain also reduces the IPNV viral load in Atlantic salmon specimens challenged with this pathogen. Our results show that oral administration of L. lactis producing Interferon I induces systemic effects in Atlantic salmon, allowing to stimulate the antiviral immune response. This probiotic could have effects against a wide variety of viruses that infect Atlantic salmon and also be effective in other salmonids due to the high identity among their type I interferons.
Assuntos
Infecções por Birnaviridae/prevenção & controle , Proteínas de Peixes/metabolismo , Imunidade Inata , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Interferon Tipo I/metabolismo , Lactococcus lactis/metabolismo , Probióticos , Salmo salar/microbiologia , Animais , Infecções por Birnaviridae/imunologia , Infecções por Birnaviridae/microbiologia , Infecções por Birnaviridae/virologia , Linhagem Celular , Proteínas de Peixes/genética , Pesqueiros , Interações Hospedeiro-Patógeno , Vírus da Necrose Pancreática Infecciosa/crescimento & desenvolvimento , Vírus da Necrose Pancreática Infecciosa/imunologia , Interferon Tipo I/genética , Lactococcus lactis/genética , Lactococcus lactis/imunologia , Proteínas de Resistência a Myxovirus/metabolismo , Salmo salar/genética , Salmo salar/imunologia , Salmo salar/virologia , Carga Viral , eIF-2 Quinase/metabolismoRESUMO
Infectious pancreatic necrosis virus (IPNV) causes economic losses in Mexican rainbow trout industry. In this study, virulence and genetic fingerprints of Mexican IPNV isolates was investigated for the first time. Two Mexican IPNV isolates were analyzed in rainbow trout fry and the Sp strain was included as high virulence. One of the Mexican IPNV isolate was obtained from diseased fish and the other from fish without clinical signs. The infection was performed using a standardized immersion. Clinical signs were observed at 4 days post infection in fry group infected with strain Sp, two days earlier than in trout infected with IPNV isolates Mexican. Severe lesions were found in 100% of the individuals of Sp group, but only in 25% of each isolated Mexican group. Results suggest that Mexican IPNV isolates are pathogenic, but less virulent than strain Sp. The amino acid motif residues of both Mexican isolates, corresponded to a subclinical disease. Nevertheless, the accumulated motility observed in the field, suggest that other factors play a role in the virulence of the disease.
Assuntos
Infecções por Birnaviridae/veterinária , Doenças dos Peixes/virologia , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Motivos de Aminoácidos , Animais , Infecções por Birnaviridae/virologia , Vírus da Necrose Pancreática Infecciosa/genética , Vírus da Necrose Pancreática Infecciosa/isolamento & purificação , México , Oncorhynchus mykiss , VirulênciaRESUMO
Infectious pancreatic necrosis (IPN) is a viral disease with considerable negative impact on the rainbow trout (Oncorhynchus mykiss) aquaculture industry. The aim of the present work was to detect genomic regions that explain resistance to infectious pancreatic necrosis virus (IPNV) in rainbow trout. A total of 2,278 fish from 58 full-sib families were challenged with IPNV and 768 individuals were genotyped (488 resistant and 280 susceptible), using a 57K SNP panel Axiom, Affymetrix. A genome-wide association study (GWAS) was performed using the phenotypes time to death (TD) and binary survival (BS), along with the genotypes of the challenged fish using a Bayesian model (Bayes C). Heritabilities for resistance to IPNV estimated using genomic information, were 0.53 and 0.82 for TD and BS, respectively. The Bayesian GWAS detected a SNP located on chromosome 5 explaining 19% of the genetic variance for TD. The proximity of Sentrin-specific protease 5 (SENP5) to this SNP makes it a candidate gene for resistance against IPNV. In case of BS, a SNP located on chromosome 23 was detected explaining 9% of the genetic variance. However, the moderate-low proportion of variance explained by the detected marker leads to the conclusion that the incorporation of all genomic information, through genomic selection, would be the most appropriate approach to accelerate genetic progress for the improvement of resistance against IPNV in rainbow trout.
Assuntos
Resistência à Doença/genética , Doenças dos Peixes/virologia , Proteínas de Peixes/genética , Vírus da Necrose Pancreática Infecciosa/fisiologia , Oncorhynchus mykiss/genética , Animais , Teorema de Bayes , Infecções por Birnaviridae/genética , Infecções por Birnaviridae/imunologia , Infecções por Birnaviridae/mortalidade , Infecções por Birnaviridae/veterinária , Doenças dos Peixes/imunologia , Doenças dos Peixes/mortalidade , Proteínas de Peixes/imunologia , Estudo de Associação Genômica Ampla , Interações Hospedeiro-Patógeno/genética , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Oncorhynchus mykiss/imunologia , Oncorhynchus mykiss/virologia , Polimorfismo de Nucleotídeo Único , Replicação Viral/fisiologiaRESUMO
The aim of this study was to compare the accuracy of breeding values (EBVs) predicted using the traditional pedigree based Best Linear Unbiased Prediction (PBLUP) and the single-step genomic Best Linear Unbiased Prediction (ssGBLUP) for resistance against infectious pancreatic necrosis virus (IPNV) in rainbow trout. A total of 2278 animals were challenged against IPNV and 768 individuals were genotyped using a 57â¯K single nucleotide polymorphism array for rainbow trout. Accuracies for both methods were assessed using five-fold cross-validation. The heritabilities were higher for PBLUP compared to ssGBLUP. The ssGBLUP accuracies outperformed PBLUP in 7 and 11% for days to death and binary survival, respectively. The ssGBLUP could be an alternative approach to improve the accuracy of breeding values for resistance against infectious pancreatic necrosis virus in rainbow trout, using information from genotyped and non-genotyped animals.
Assuntos
Infecções por Birnaviridae/genética , Resistência à Doença , Doenças dos Peixes/genética , Estudo de Associação Genômica Ampla/métodos , Seleção Artificial , Truta/genética , Animais , Infecções por Birnaviridae/imunologia , Doenças dos Peixes/imunologia , Estudo de Associação Genômica Ampla/normas , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Truta/virologiaRESUMO
In order to obtain an insight into genomic changes and associated evolution and adaptation of Infectious Pancreatic Necrosis Virus (IPNV), the complete coding genomes of 57 IPNV isolates collected from Scottish aquafarms from 1982 to 2014 were sequenced and analysed. Phylogenetic analysis of the sequenced IPNV strains showed separate clustering of genogroups I, II, III and V. IPNV isolates with genetic reassortment of segment A/B of genogroup III/II were determined. About 59â% of the IPNV isolates belonged to the persistent type and 32â% to the low-virulent type, and only one highly pathogenic strain (1.79â%) was identified. Codon adaptation index calculations indicated that the IPNV major capsid protein VP2 has adapted to its salmonid host. Under-representation of CpG dinucleotides in the IPNV genome to minimize detection by the innate immunity receptors, and observed positive selection in the virulence determination sites of VP2 embedded in the variable region of the main antigenic region, suggest an immune escape mechanism driving virulence evolution. The prevalence of mostly persistent genotypes, together with the assumption of adaptation and immune escape, indicates that IPNV is evolving with the host.
Assuntos
Infecções por Birnaviridae/veterinária , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/virologia , Variação Genética , Vírus da Necrose Pancreática Infecciosa/classificação , Vírus da Necrose Pancreática Infecciosa/genética , Adaptação Biológica , Animais , Aquicultura , Infecções por Birnaviridae/epidemiologia , Infecções por Birnaviridae/virologia , Proteínas do Capsídeo/genética , Códon , Genótipo , Evasão da Resposta Imune , Vírus da Necrose Pancreática Infecciosa/isolamento & purificação , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Epidemiologia Molecular , Prevalência , Escócia/epidemiologia , Seleção Genética , Análise de Sequência de DNA , Virulência , Sequenciamento Completo do GenomaRESUMO
Infectious pancreatic necrosis (IPN) is a significant disease of farmed salmonids resulting in direct economic losses due to high mortality in China. However, no gene sequence of any Chinese infectious pancreatic necrosis virus (IPNV) isolates was available. In the study, moribund rainbow trout fry samples were collected during an outbreak of IPN in Yunnan province of southwest China in 2013. An IPNV was isolated and tentatively named ChRtm213. We determined the full genome sequence of the IPNV ChRtm213 and compared it with previously identified IPNV sequences worldwide. The sequences of different structural and non-structural protein genes were compared to those of other aquatic birnaviruses sequenced to date. The results indicated that the complete genome sequence of ChRtm213 strain contains a segment A (3099 nucleotides) coding a polyprotein VP2-VP4-VP3, and a segment B (2789 nucleotides) coding a RNA-dependent RNA polymerase VP1. The phylogenetic analyses showed that ChRtm213 strain fell within genogroup 1, serotype A9 (Jasper), having similarities of 96.3% (segment A) and 97.3% (segment B) with the IPNV strain AM98 from Japan. The results suggest that the Chinese IPNV isolate has relative closer relationship with Japanese IPNV strains. The sequence of ChRtm213 was the first gene sequence of IPNV isolates in China. This study provided a robust reference for diagnosis and/or control of IPNV prevalent in China.
Assuntos
Doenças dos Peixes/genética , Vírus da Necrose Pancreática Infecciosa/genética , Oncorhynchus mykiss/virologia , Sequência de Aminoácidos/genética , Animais , Sequência de Bases , China , Doenças dos Peixes/virologia , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Anotação de Sequência Molecular , Oncorhynchus mykiss/genética , FilogeniaRESUMO
Reassortment is one of the main mechanisms of evolution in dsRNA viruses with segmented genomes. It contributes to generate genetic diversity and plays an important role in the emergence and spread of new strains with altered virulence. Natural reassorment has been demonstrated among infectious pancreatic necrosis-like viruses (genus Aquabirnavirus, Birnaviridae). In the present study, coinfections between different viral strains, and genome sequencing by the Sanger and Illumina methods were applied to analyze the frequency of reassortment of this virus in vitro, the possible mechanisms involved, and its effect on virulence. Results have demonstrated that reassortment is a cell-dependent and non-random process, probably through differential expression of the different mRNA classes in the ribosomes of a specific cell, and by specific associations between the components to construct the ribonucleoprotein (RNP) complexes and/or RNP cross-inhibition. However, the precise mechanisms involved, known in other viruses, still remain to be demonstrated in birnaviruses.
Assuntos
Infecções por Birnaviridae/veterinária , Doenças dos Peixes/virologia , Vírus da Necrose Pancreática Infecciosa/genética , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Vírus Reordenados/patogenicidade , Recombinação Genética , Animais , Sequência de Bases , Infecções por Birnaviridae/virologia , Vírus da Necrose Pancreática Infecciosa/fisiologia , Vírus Reordenados/genética , Vírus Reordenados/fisiologia , VirulênciaRESUMO
Infectious pancreatic necrosis (IPN) is a contagious viral disease of fish that causes economic losses in aquaculture worldwide. In Finland, IPN virus (IPNV) has been isolated since 1987 from adult fish showing no signs of clinical disease at fish farms located in the coastal areas of the Baltic Sea. The inland area of Finland, however, remained free of IPN until 2012, when fish on several rainbow trout farms were diagnosed IPNV-positive. The fish mortalities detected at the farms were low, but clinical signs and histopathological changes typical for IPNV infection were seen in juvenile salmonids. IPNV was isolated at high water temperatures up to 22°C. In 2013 and 2014, IPNV detections continued at inland farms, indicating that infections have spread. The aim of this study was to describe the epidemiology of the outbreak and to characterise the Finnish inland IPNV isolates using histopathological, immunohistochemical and genetic approaches. In order to determine the epidemiological origin of the inland IPNV infections, the partial viral capsid protein (VP2) gene sequences of the inland IPNV isolates were compared with the sequences of the isolates from the coastal farms. Based on the genetic analysis, the inland isolates belong to IPNV Genogroup 2 (Serotype A3/Ab), and the origin of the isolates appears to be one or several coastal fish farms.
Assuntos
Aquicultura , Infecções por Birnaviridae/veterinária , Doenças dos Peixes/virologia , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Oncorhynchus mykiss , Animais , Infecções por Birnaviridae/epidemiologia , Infecções por Birnaviridae/virologia , Finlândia/epidemiologia , Doenças dos Peixes/epidemiologia , Vírus da Necrose Pancreática Infecciosa/genética , FilogeniaRESUMO
Goldfish Carassius auratus are commonly used in scientific research and have a significant economic value in the pet trade. In this study, two cell lines were established from the snout and kidney tissue of goldfish, in order to create a biological monitoring tool for viral diseases. Cell lines were optimally maintained at 25 °C in M199 medium supplemented with 15-20 % fetal bovine serum. Chromosomal analysis indicated that both cell lines remained diploid, with a mean chromosomal count of 100. Results of viral inoculation assays revealed that both cell lines shared similar patterns of viral susceptibility and production to infectious pancreatic necrosis virus (IPNV). The viral titers of IPNV in goldfish snout cell line (GFSe) and goldfish kidney cell line (GFKf) reached 10(6.8) and 10(5.9) TCID50/0.1 mL, respectively, within 7 days. The cytopathic effect could be observed when Cyprinid herpesvirus 2 was inoculated into these cells; however, the CPE disappeared after four passages. No CPE was observed in the cells infected by spring viremia carp virus or grass carp reovirus. These newly established cell lines will be a useful diagnostic tool for viral diseases in this fish species and also for the isolation and study of goldfish viruses in future.
Assuntos
Linhagem Celular/virologia , Carpa Dourada , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Rim/citologia , Nariz/citologia , Animais , Infecções por Birnaviridae/veterinária , Proliferação de Células , Células Cultivadas , Cromossomos , Doenças dos PeixesRESUMO
Due to their direct antiviral activity, Mx proteins play a main role in the response mediated by type I interferon against viral infections. The study on gilthead seabream Mx proteins is especially interesting, since this species is unusually resistant to viral diseases, being asymptomatic carrier of several viruses pathogenic to other fish species. Gilthead seabream has three Mx proteins (Mx1, Mx2 and Mx3) that, separately, display antiviral activity against a wide range of viruses, showing interesting differences in their antiviral specificities. In this work, the possible synergy between the three Mx isoforms has been studied using in vitro systems consisting of CHSE-214 cells stably expressing two or the three gilthead seabream Mx proteins. The antiviral activity of these Mx combinations has been tested against the Infectious Pancreatic Necrosis Virus (IPNV), the Viral Haemorrhagic Septicaemia Virus (VHSV), the European Sheatfish Virus (ESV) and the Lymphocystis Disease Virus (LCDV). A synergistic effect of the Mx proteins was only detected against ESV, no synergy was observed against LCDV, and a negative interference was detected against the two RNA viruses tested, IPNV and VHSV, as viral replication was higher in cells expressing certain Mx combinations than in cells expressing these proteins separately. These results suggest a functional interaction between gilthead seabream Mx isoforms, which results in a higher or lower antiviral activity depending on the virus tested, thus supporting the idea of complex virus-host interactions and finely tuned mechanisms controlling the antiviral activity of Mx proteins.
Assuntos
Antivirais/imunologia , Proteínas de Peixes/imunologia , Proteínas de Resistência a Myxovirus/imunologia , Dourada/imunologia , Animais , Linhagem Celular , Efeito Citopatogênico Viral , Proteínas de Peixes/genética , Interações Hospedeiro-Patógeno/genética , Interações Hospedeiro-Patógeno/imunologia , Vírus da Necrose Pancreática Infecciosa/imunologia , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Iridoviridae/imunologia , Iridoviridae/patogenicidade , Proteínas de Resistência a Myxovirus/genética , Novirhabdovirus/imunologia , Novirhabdovirus/patogenicidade , Ranavirus/imunologia , Ranavirus/patogenicidade , Dourada/genética , Dourada/virologiaRESUMO
Infectious pancreatic necrosis virus (IPNV) is a prevalent pathogen in fish worldwide. The virus causes substantial mortality in Atlantic salmon juveniles and smolts when transferred to sea water and persistent infection in surviving fish after disease outbreaks. Here, we have investigated the occurrence of the virus as well as the innate immune marker Mx in the head kidney (HK) of Atlantic salmon throughout an experimental challenge covering both a fresh and a seawater phase. The fish were challenged with a high (HV) and low virulence (LV) IPNV. Both isolates caused mortality due to reactivation of the virus after transfer to sea water. In the freshwater phase, higher levels of virus transcripts were detected in the HK of fish infected with LV IPNV compared to HV, suggesting that the HV isolate is able to limit its own replication to a level where the innate immune system is not alerted. Further, ex vivoHK leucocytes derived from fish infected with the two isolates were stimulated with CpG DNA. Significantly, higher IFN levels were found in the LV compared to the HV group in the freshwater phase. This suggests that the viruses attenuate the antiviral host immune response at different levels which may contribute to the observed differences in disease outcome.
Assuntos
Infecções por Birnaviridae/veterinária , Doenças dos Peixes/microbiologia , Interações Hospedeiro-Patógeno/imunologia , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Salmo salar/microbiologia , Animais , Infecções por Birnaviridae/microbiologia , Infecções por Birnaviridae/mortalidade , Doenças dos Peixes/mortalidade , Dados de Sequência Molecular , Proteínas de Resistência a Myxovirus/metabolismoRESUMO
We have studied stress-induced reversion to virulence of infectious pancreatic necrosis virus (IPNV) in persistently infected Atlantic salmon (Salmo salar L.) fry. Naïve fry were persistently infected with a virulent strain (T(217)A(221) of major structural virus protein 2, VP2) or a low virulent (T(217)T(221)) variant of IPNV. The fry were infected prior to immunocompetence as documented by lack of recombination activating gene-1, T-cell receptor and B-cell receptor mRNA expression at time of challenge. The fish were followed over 6 months and monitored monthly for presence of virus and viral genome mutations. No mutation was identified in the TA or TT group over the 6 months period post infection. Six months post infection TA and TT infected groups were subject to daily stress for 7 days and then sampled weekly for an additional period of 28 days post stress. Stress-responses were documented by down-regulation of mRNA expression of IFN-α1 and concomitant increase of replication levels of T(217)T(221) infected fish at day 1 post stress. By 28 days post stress a T221A reversion was found in 3 of 6 fish in the T(217)T(221) infected group. Sequencing of reverted isolates showed single nucleotide peaks on chromatograms for residue 221 for all three isolates and no mix of TA and TT strains. Replication fitness of reverted (TA) and non-reverted (TT) variants was studied in vitro under an antiviral state induced by recombinant IFN-α1. The T(217)A(221) reverted variant replicated to levels 23-fold higher than the T(217)T(221) strain in IFN-α1 treated cells. Finally, reverted TA strains were virulent when tested in an in vivo trial in susceptible salmon fry. In conclusion, these results indicate that stress plays a key role in viral replication in vivo and can facilitate conditions that will allow reversion from attenuated virus variants of IPNV.
Assuntos
Infecções por Birnaviridae/veterinária , Doenças dos Peixes/virologia , Pancreatopatias/veterinária , Salmo salar/virologia , Ativação Viral , Animais , Sequência de Bases , Infecções por Birnaviridae/imunologia , Infecções por Birnaviridae/mortalidade , Infecções por Birnaviridae/virologia , Proteínas do Capsídeo/química , Proteínas do Capsídeo/genética , Células Cultivadas , Doenças dos Peixes/imunologia , Doenças dos Peixes/mortalidade , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Genes Virais , Genótipo , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Ligação de Hidrogênio , Imunoglobulina M/genética , Imunoglobulina M/metabolismo , Vírus da Necrose Pancreática Infecciosa/genética , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Vírus da Necrose Pancreática Infecciosa/fisiologia , Interferon-alfa/genética , Interferon-alfa/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Oncorhynchus mykiss , Pancreatopatias/imunologia , Pancreatopatias/mortalidade , Pancreatopatias/virologia , Conformação Proteica , Receptores de Antígenos de Linfócitos T/genética , Receptores de Antígenos de Linfócitos T/metabolismo , Salmo salar/imunologia , Análise de Sequência de DNA , Estresse Fisiológico , Virulência/genética , Replicação ViralRESUMO
In order to study the variety of infectious pancreatic necrosis virus (IPNV) strains involved in outbreaks of infectious pancreatic necrosis (IPN) in Atlantic salmon fish farms, samples were collected from 19 different outbreaks of IPN in the northern part of Norway. The main objective of this study was to examine whether IPNV isolates of different virulence were involved in the outbreaks and could explain the variable IPN protection observed in vaccinated post-smolts in the field. Both the molecular basis of virulence of all field isolates and virulence expressed by mortality after bath challenge of unvaccinated post-smolts with eight of the isolates were studied. Very little variation among the field isolates was detected when the 578-bp variable region encoding the VP2 protein known to be involved in virulence was sequenced. The cumulative mortality after experimental challenge with field isolates genetically characterized as highly virulent was always high (40-56%), while the cumulative mortality of the same strains in vaccinated post-smolts during the field outbreaks varied from 1 to 50%. Although the tested samples came from fish vaccinated with the same vaccine product, the protection against IPN varied. These results demonstrate that differences in virulence of the isolates were not the main reason for the variation in mortality in the field outbreaks. Most of the field isolates were of high virulence, which is shown in experimental challenges to be important for mortality, but clearly other factors that might affect the susceptibility of IPN also play an important role in the outcome of an IPNV infection.
Assuntos
Infecções por Birnaviridae/veterinária , Doenças dos Peixes/virologia , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Sequência de Aminoácidos , Animais , Infecções por Birnaviridae/mortalidade , Infecções por Birnaviridae/virologia , Doenças dos Peixes/mortalidade , Peixes , Vírus da Necrose Pancreática Infecciosa/genética , Vírus da Necrose Pancreática Infecciosa/isolamento & purificação , Dados de Sequência Molecular , Noruega , Alinhamento de Sequência , Proteínas Estruturais Virais/genética , Virulência/genéticaRESUMO
The possible importance of the O-linked glycosylation in virion stability and infectivity of infectious pancreatic necrosis virus (IPNV) was analysed. Enzymatic treatment with O-glycosidase of radiolabelled virions under different ionic conditions, to allow for possible alternative exposure of glycosidic enzyme cleavage sites, did not alter the specific infectivity of virions re-isolated after rate-zonal centrifugation in glycerol gradients. As an alternative method to assess the significance of carbohydrates in IPNV integrity, periodate oxidation in the presence of an aldehyde quencher was chosen. Following re-isolation of viruses, a 3-5 (10)log-unit reduction in specific infectivity was revealed and, at higher concentrations, a total disruption or virion aggregation was observed. The loss of infectivity of intact virions was not because of a lack of attachment to cells. Additionally, re-evaluation of reading values from UV-spectra of purified IPNV yielded a specific infectivity of 3 × 10(11) TCID(50)-units mg(-1) of protein and a ratio of 40 virions per TCID(50)-unit in the CHSE-214 cell system.
Assuntos
Instabilidade Genômica/efeitos dos fármacos , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Vírion/metabolismo , Animais , Células Cultivadas , Colorimetria , Embrião não Mamífero/citologia , Glicosídeo Hidrolases/farmacologia , Glicosilação , Lectinas/metabolismo , Salmão , Espectrofotometria Ultravioleta , Vírion/genéticaRESUMO
BACKGROUND: Infectious pancreatic necrosis virus (IPNV) is an aquatic member of the Birnaviridae family that causes widespread disease in salmonids. IPNV is represented by multiple strains with markedly different virulence. Comparison of isolates reveals hyper variable regions (HVR), which are presumably associated with pathogenicity. However little is known about the rates and modes of sequence divergence and molecular mechanisms that determine virulence. Also how the host response may influence IPNV virulence is poorly described. METHODS: In this study we compared two field isolates of IPNV (NFH-Ar and NFH-El). The sequence changes, replication and mortality were assessed following experimental challenge of Atlantic salmon. Gene expression analyses with qPCR and microarray were applied to examine the immune responses in head kidney. RESULTS: Significant differences in mortality were observed between the two isolates, and viral load in the pancreas at 13 days post infection (d p.i.) was more than 4 orders of magnitude greater for NFH-Ar in comparison with NFH-El. Sequence comparison of five viral genes from the IPNV isolates revealed different mutation rates and Ka/Ks ratios. A strong tendency towards non-synonymous mutations was found in the HRV of VP2 and in VP3. All mutations in VP5 produced precocious stop codons. Prior to the challenge, NFH-Ar and NFH-El possessed high and low virulence motifs in VP2, respectively. Nucleotide substitutions were noticed already during passage of viruses in CHSE-214 cells and their accumulation continued in the challenged fish. The sequence changes were notably directed towards low virulence. Co-ordinated activation of anti-viral genes with diverse functions (IFN-a1 and c, sensors - Rig-I, MDA-5, TLR8 and 9, signal transducers - Srk2, MyD88, effectors - Mx, galectin 9, galectin binding protein, antigen presentation - b2-microglobulin) was observed at 13 d p.i. (NFH-Ar) and 29 d p.i. (both isolates). CONCLUSIONS: Mortality and expression levels of the immune genes were directly related to the rate of viral replication, which was in turn associated with sequences of viral genes. Rapid changes in the viral genome that dramatically reduced virus proliferation might indicate a higher susceptibility to protective mechanism employed by the host. Disease outbreak and mortality depend on a delicate balance between host defence, regulation of signalling cascades and virus genomic properties.
Assuntos
Infecções por Birnaviridae/imunologia , Doenças dos Peixes/imunologia , Vírus da Necrose Pancreática Infecciosa/imunologia , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Mutação , Salmo salar/virologia , Animais , Infecções por Birnaviridae/mortalidade , Infecções por Birnaviridae/patologia , Infecções por Birnaviridae/virologia , Doenças dos Peixes/mortalidade , Doenças dos Peixes/patologia , Doenças dos Peixes/virologia , Perfilação da Expressão Gênica , Vírus da Necrose Pancreática Infecciosa/genética , Vírus da Necrose Pancreática Infecciosa/isolamento & purificação , Rim/virologia , Análise em Microsséries , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sobrevida , Virulência , Replicação ViralRESUMO
The in vitro infectivity and genotype of three IPNV strains (V70, V112 and V98) was linked to the level of transcript synthesis for the Mx3 protein in RTG-2 (Rainbow trout gonad) cells and in Salmo salar. The V70 and V98 strains corresponded to the Sp genotype, whilst the V112 corresponded to VR-299; the presence of Pro-217 and Ala-221 in VP2 identified V70 as a strain of medium virulence level whilst V112 (Ala-217 and Thr-221) and V98 (Pro-217 and Thr-221) were of low virulence. This is concurrent with several in vitro tests which showed V70 to be a strain with highly infectivity (P<0.05). In both the in vitro and in vivo trials, the strains demonstrated the induction of the Mx transcript, although no differences were detected, and the level always were significantly lesser that observed in poly I:C samples. The results suggest that the infectivity observed is related to the presence of certain specific residues in VP2, and that neither the infectivity nor the genotype appears to bear any relation to Mx induction capacity.
Assuntos
Infecções por Birnaviridae/veterinária , Doenças dos Peixes/virologia , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Salmo salar , Sequência de Aminoácidos , Animais , Infecções por Birnaviridae/metabolismo , Infecções por Birnaviridae/virologia , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/metabolismo , Linhagem Celular , Doenças dos Peixes/metabolismo , Regulação Viral da Expressão Gênica , Genótipo , Vírus da Necrose Pancreática Infecciosa/genética , Dados de Sequência Molecular , Replicação ViralRESUMO
Persistent infection by IPNV was induced in RTG-2 and RTG-P1 cells in vitro and the influence of this phenomenon on viral infectivity, viral antigen expression and interference with homologous and heterologous viruses was characterized over successive passages. The induction of IFN was also assessed, as was the sequence of the VP2 viral capsid protein, the region believed to be responsible for virulence, attenuation or persistence. Viral antigen expression was recorded in cells with no evidence of cytopathic effects and in these conditions, flow cytometry was more sensitive than RT-PCR to demonstrate the presence of a non-lytic virus. Interference of homologous viral infection could be detected in cross-infection experiments and in RTG-P1 cells persistently infected with IPNV, the Mx1 promoter could still be activated for at least 5 successive passages. Indeed, although over-induction of luciferase was not observed by re-infection with homologous or heterologous viruses, a significant increase in luciferase was induced by poly I:C. IFN transcripts could be quantified by qRT-PCR in the persistent cells at several passages, suggesting that IFN plays a role in maintaining IPNV persistence. In addition, we observed the same determinants in the VP2 sequences from the persistent virus as those described previously for IPNV adaptation and persistence in cell culture.
Assuntos
Infecções por Birnaviridae/veterinária , Doenças dos Peixes/imunologia , Doenças dos Peixes/virologia , Vírus da Necrose Pancreática Infecciosa/imunologia , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Sequência de Aminoácidos , Animais , Antígenos Virais/genética , Antígenos Virais/metabolismo , Sequência de Bases , Infecções por Birnaviridae/imunologia , Infecções por Birnaviridae/virologia , Linhagem Celular , Primers do DNA/genética , DNA Viral/genética , Vírus da Necrose Pancreática Infecciosa/genética , Vírus da Necrose Pancreática Infecciosa/fisiologia , Interferons/biossíntese , Interferons/genética , Dados de Sequência Molecular , Oncorhynchus mykiss , Perciformes , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Homologia de Sequência de Aminoácidos , Interferência Viral , Proteínas Estruturais Virais/genética , Proteínas Estruturais Virais/imunologia , Proteínas Estruturais Virais/metabolismo , VirulênciaRESUMO
Viral hemorrhagic septicemia virus (VHSV) and infectious pancreatic necrosis virus (IPNV) are two rainbow trout (Oncorhynchus mykiss) pathogens. While IPNV is known to be vertically transmitted to the next generation through the oocyte, VHSV is known to replicate in the ovary and be transmitted horizontally through the ovarian fluid. In this work, we wanted to study whether these differences had an effect on the immune response triggered in the ovary, with a focus on the chemokine response. We have studied the kinetics of viral gene expression and the sites of replication, confirming that great differences exist between the replication of the two viruses in the gonad. Next, we studied the levels of expression of several CXC and CC chemokines in the ovary and found that while VHSV strongly triggered chemokine transcription, IPNV had almost no effect. This lack of immune response might be an advantage that permits its vertical transmission.
Assuntos
Infecções por Birnaviridae/imunologia , Septicemia Hemorrágica/imunologia , Vírus da Necrose Pancreática Infecciosa/fisiologia , Novirhabdovirus/fisiologia , Animais , Infecções por Birnaviridae/transmissão , Infecções por Birnaviridae/virologia , Células Cultivadas , Quimiocinas/genética , Quimiocinas/metabolismo , Feminino , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Septicemia Hemorrágica/transmissão , Septicemia Hemorrágica/virologia , Imuno-Histoquímica , Vírus da Necrose Pancreática Infecciosa/patogenicidade , Novirhabdovirus/patogenicidade , Oncorhynchus mykiss , Técnicas de Cultura de Órgãos , Ovário/imunologia , Ovário/metabolismo , Ovário/patologia , Replicação ViralRESUMO
Infectious pancreatic necrosis virus (IPNV), a pathogen of salmon and trout, imposes a severe toll on the aquaculture and sea farming industries. IPNV belongs to the Aquabirnavirus genus in the Birnaviridae family of bisegmented double-stranded RNA viruses. The virions are nonenveloped with a T=13l icosahedral capsid made by the coat protein VP2, the three-dimensional (3D) organization of which is known in detail for the family prototype, the infectious bursal disease virus (IBDV) of poultry. A salient feature of the birnavirus architecture is the presence of 260 trimeric spikes formed by VP2, projecting radially from the capsid. The spikes carry the principal antigenic sites as well as virulence and cell adaptation determinants. We report here the 3.4-A resolution crystal structure of a subviral particle (SVP) of IPNV, containing 20 VP2 trimers organized with icosahedral symmetry. We show that, as expected, the SVPs have a very similar organization to the IBDV counterparts, with VP2 exhibiting the same overall 3D fold. However, the spikes are significantly different, displaying a more compact organization with tighter packing about the molecular 3-fold axis. Amino acids controlling virulence and cell culture adaptation cluster differently at the top of the spike, i.e., in a central bowl in IBDV and at the periphery in IPNV. In contrast, the spike base features an exposed groove, conserved across birnavirus genera, which contains an integrin-binding motif. Thus, in addition to revealing the viral antigenic determinants, the structure suggests that birnaviruses interact with different receptors for attachment and for cell internalization during entry.