Serological tests fail to discriminate dogs with visceral leishmaniasis that transmit Leishmania infantum to the vector Lutzomyia longipalpis
Rev. Soc. Bras. Med. Trop
; Rev. Soc. Bras. Med. Trop;50(4): 483-488, July-Aug. 2017. tab
Article
en En
| LILACS
| ID: biblio-896987
Biblioteca responsable:
BR1.1
ABSTRACT
Abstract INTRODUCTION The control of reservoirs for Leishmania infantum -induced zoonotic visceral leishmaniasis requires the identification of dogs posing a population risk. Here, we assessed the performance of several assays to identify Lutzomyia longipalpis infectious dogs. METHODS We evaluated 99 dogs that were positive for visceral leishmaniasis based on parasite identification. Serological analyses were performed using an enzyme-linked immunosorbent assay, immunofluorescence antibody tests in 140 and 180 dilutions, rapid dual path platform tests, immunochromatographic assay with a recombinant rK39 antigen, fast agglutination screening tests, and direct agglutination tests. We also performed PCR to analyze peripheral blood and xenodiagnosis. RESULTS Forty-six dogs infected at least one L. longipalpis specimen. Although the serological test sensitivities were above 85% for detecting L. longipalpis infectious dogs, none showed a satisfactory performance, as both specificity (0.06 to 13%) and the area under the receiver operating characteristic curve (45 to 53%) were low. The PCR results were also weak, with a sensitivity of 30%, specificity of 72%, and an area under the receiver operating characteristic curve of 51%. The infected L. longipalpis proportion was higher among asymptomatic dogs than symptomatic dogs. Among the symptomatic dogs, those with ulceration-free skin diseases were more infectious, with an odds ratio of 9.3 (confidence interval of 1.10 - 428.5). The larger the number of insects fed, the greater the detected infectiousness. CONCLUSIONS Our study supports the imperative to develop novel technologies for identifying the infectious dogs that transmit L. infantum for the benefit of public health.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Contexto en salud:
2_ODS3
/
3_ND
Problema de salud:
2_enfermedades_transmissibles
/
3_zoonosis
Asunto principal:
Psychodidae
/
Pruebas Serológicas
/
Anticuerpos Antiprotozoarios
/
Leishmania infantum
/
Enfermedades de los Perros
/
Mosquitos Vectores
/
Leishmaniasis Visceral
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Jornal brasileiro de medicina tropical
/
Rev Soc Bras Med Trop
/
Rev. Soc. Bras. Med. Trop
/
Rev. Soc. Bras. Med. Trop. (Online)
/
Revista da Sociedade Brasileira de Medicina Tropical (Impresso)
Asunto de la revista:
MEDICINA TROPICAL
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil