Pancreatic islet isolation by mechanical-enzymatic separation, stationary collagenase digestion and dextran discontinuous density gradient purification: experimental study in dogs
Acta cir. bras
; 17(2): 89-94, mar.-abr. 2002. ilus, tab
Article
en En
| LILACS
| ID: lil-308675
Biblioteca responsable:
BR1.2
ABSTRACT
The prospects for allotransplantation of pancreatic islets in man depend on the development of methods that provide sufficient quantities of pancreatic islets from a single donor, which are capable, when transplanted, of achieve the normalization of carbohydrate metabolism. Objective:
Evaluate the efficacy of the isolation of Langerhans islets from dogs, by means of mechanical-enzymatic separation technique with stationary digestion using collagenase, and purification with a discontinuous dextran density gradient.Methods:
The counting of islet numbers and evaluation of their sizes was accomplished by staining with diphenylthiocarbazone and using stereoscopic microscopes equipped with eyepiece reticule for the measurement of average diameters of stained islets.Results:
The results disclosed that the average number of islets isolated was 81032.20 ñ 24736.79 and the average number of islets isolated per kg of body weight was 6938.70 1392.43. The average number of islets isolated per kg of body weight showed significant correlation with body weight and weight of the pancreas resected.Conclusion:
The number of islets isolated, of a single donor, by mechanical-enzymatic separation, stationary collagenase digestion and discontinuous dextran density gradient purification can be sufficient to success of pancreatic islets transplant in dogs.
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Trasplante de Islotes Pancreáticos
/
Islotes Pancreáticos
/
Dextranos
/
Colagenasas
/
Diabetes Mellitus
/
Anticoagulantes
Límite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Asunto de la revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2002
Tipo del documento:
Article
País de afiliación:
Brasil