Cellulase activity of a Lentinula edodes (Berk.) Pegl. strain grown in media containing Carboximetilcellulose or microcrystalline cellulose
Braz. arch. biol. technol
; 46(3): 333-337, Jun. 2003. graf
Artículo
en Inglés
| LILACS
| ID: lil-351409
Biblioteca responsable:
BR1.1
ABSTRACT
Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel media. This enzyme was detected in mycelial extracts only. With 1.7 percent Avicel liquid medium, the strain did not produce exocellobiohydrolase, but 74 æmol RBBR/mg protein/min was detected with 0.5 percent Avicel. The substitution of Avicel by 0.5 percent CMC reduced this activity. Endoglucanase also had maximum activity in 0.5 percent Avicel medium (approximately 820 UI/mg protein) after 96 h incubation. In supernatants from 0.5 percent CMC, the maximum activity attained was 200 UI/mg protein only
Texto completo:
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Colección:
Bases de datos internacionales
Base de datos:
LILACS
Idioma:
Inglés
Revista:
Braz. arch. biol. technol
Asunto de la revista:
Biologia
Año:
2003
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal Rural de Pernambuco/BR
/
Universidade Federal de Pernambuco/BR
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