Molecular detection of human astrovirus in an urban sewage treatment plant in Rio de Janeiro, Brazil
Mem. Inst. Oswaldo Cruz
; 103(8): 819-823, Dec. 2008. tab
Article
en En
| LILACS
| ID: lil-502303
Biblioteca responsable:
BR1.1
ABSTRACT
The objective of this study was to evaluate the prevalence and dissemination of human astroviruses (HAstV) in the environment by analyzing urban sewage samples from a wastewater treatment plant in the city of Rio de Janeiro, Brazil. A one-year study was performed with a total of 48 raw and treated sewage composite samples, which were collected biweekly from an activated sludge plant. Virus particles were concentrated by the adsorption-elution method using negatively charged membranes associated to a Centriprep Concentrator® 50 (Nihon Millipore). HAstV were detected in 16.7 percent of the samples in raw and treated sewage by using both qualitative and quantitative reverse transcriptase-polymerase chain reactions (RT-PCR and qPCR, respectively). Positive untreated sewage sample exhibited mean values of 1.1 x 10(4) gEq/mL. The qPCR sensitivity was 18 gEq/reaction. Through utilization of qPCR, a HAstV recovery efficiency of 4.2 percent and 4.3 percent was demonstrated for raw and treated sewage samples, respectively. The presence of HAstV in both the raw and treated sewage samples demonstrated the dissemination of these viruses in the environment as well as viral permanence after sewage treatment. There was a reduction in the total and faecal coliform levels, indicating efficiency of the wastewater treatment plant.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Contexto en salud:
12_ODS3_hazardous_contamination
/
2_ODS3
Problema de salud:
12_water_sanitation_hygiene
/
2_quimicos_contaminacion
Asunto principal:
Mamastrovirus
/
Aguas del Alcantarillado
/
Microbiología del Agua
/
Purificación del Agua
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
/
Risk_factors_studies
Límite:
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
2008
Tipo del documento:
Article
/
Project document
País de afiliación:
Brasil