DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis
J. venom. anim. toxins incl. trop. dis
; 17(1): 98-102, 2011. ilus, tab
Article
en En
| LILACS
| ID: lil-576888
Biblioteca responsable:
BR33.1
ABSTRACT
This study aims to report the amplification of the DNA of Leishmania (V.) braziliensis, using polymerase chain reaction, obtained from the saliva of a patient with American cutaneous leishmaniasis who did not present any lesion in the oral mucosa. Amplification produced fragments of 103 bp, an estimated size employing Leishmania (V.) braziliensis primers (b1 e b2). The present results revealed, for the first time, that the in vitro amplification of Leishmania DNA using samples from the salivary fluid of a patient with American cutaneous leishmaniasis is possible. However, more studies are required with a larger number of participants to evaluate the usefulness of saliva as a non-invasive sample for PCR. The development of such non-invasive technique is necessary for the diagnosis of many diseases in the future, especially infectious and parasitic ones.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Saliva
/
Leishmania braziliensis
/
ADN
/
Leishmaniasis Cutánea
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J. venom. anim. toxins incl. trop. dis
Asunto de la revista:
TOXICOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Brasil