High similarity of Trypanosoma cruzi kDNA genetic profiles detected by LSSP-PCR within family groups in an endemic area of Chagas disease in Brazil
Rev. Soc. Bras. Med. Trop
; 47(5): 653-656, Sep-Oct/2014. graf
Article
en En
| LILACS
| ID: lil-728908
Biblioteca responsable:
BR1.1
ABSTRACT
Introduction Determining the genetic similarities among Trypanosoma cruzi populations isolated from different hosts and vectors is very important to clarify the epidemiology of Chagas disease. Methods An epidemiological study was conducted in a Brazilian endemic area for Chagas disease, including 76 chronic chagasic individuals (96.1% with an indeterminate form; 46.1% with positive hemoculture). Results T. cruzi I (TcI) was isolated from one child and TcII was found in the remaining (97.1%) subjects. Low-stringency single-specific-primer-polymerase chain reaction (LSSP-PCR) showed high heterogeneity among TcII populations (46% of shared bands); however, high similarities (80-100%) among pairs of mothers/children, siblings, or cousins were detected. Conclusions LSSP-PCR showed potential for identifying similar parasite populations among individuals with close kinship in epidemiological studies of Chagas disease. .
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Contexto en salud:
3_ND
Problema de salud:
3_chagas_disease
/
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Trypanosoma cruzi
/
ADN Protozoario
/
Enfermedad de Chagas
/
ADN de Cinetoplasto
Límite:
Adolescent
/
Adult
/
Child
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Child, preschool
/
Female
/
Humans
/
Male
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
MEDICINA TROPICAL
Año:
2014
Tipo del documento:
Article
/
Project document
País de afiliación:
Brasil