Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats
Acta cir. bras
; 31(1): 22-27, Jan. 2016. tab, graf
Article
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| LILACS
| ID: lil-771851
Biblioteca responsable:
BR1.1
ABSTRACT
PURPOSE:
To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model.METHODS:
Ten female Wistar rats were divided randomly in two groups. Group A - ADR; Group B - FA+ADR. Dams from group B received daily, since two weeks before the pregnancy to the end of pregnancy, FA (50mg/kg) by gavage. Dams from both groups received ADR (6mk/kg) by intraperitoneal injection on gestational day (GD) 8. Their fetuses were harvested by cesarean section on GD21 and were examined looking for ARMs. The thickness of anal stratified squamous epithelium (ASSE) and intestinal epithelium (IE) were analyzed. p≤0.05*.RESULTS:
81 fetuses were harvested. The number of fetuses; number of ARMs; mean (∆%) (± SD) were determined to be, respectively ADR - 41[29;65%(±37%)] versus FA+ADR - 40[04;16%(±36%)] (p=0.05). AMRs were significantly lower in FA+ADR group than in ADR group (p=0.05). The thickness (µm) of ASSE (± SD) and IE (± SD) were measured, respectively ADR - [25.98(±0.74) and 19.48(±1.68)] versus FA+ADR - [24.74(±0.91) and 24.80(±0.81)] (p<0.005). The thickness of IE was significantly enlarged when FA was given (p<0.005).CONCLUSIONS:
Single dose of adriamycin on D8 was able to induce anorectal malformations. Folic acid reduces the number and enlarged the IE of ARMs ADR-induced.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Ano Imperforado
/
Ácido Fólico
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Acta cir. bras
Asunto de la revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2016
Tipo del documento:
Article
País de afiliación:
Brasil