Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro
Acta cir. bras
; 31(2): 119-125, Feb. 2016. graf
Article
en En
| LILACS
| ID: lil-775562
Biblioteca responsable:
BR1.1
ABSTRACT
PURPOSE:
To detect whether chitin and sepia ink sponge (CS) can promote wound healing and elevate impact of CS on phagocytosis ability of macrophages.METHODS:
Forty-eight rats were assigned to four groups Normal group (Normal), negative control group (Con), chitin and sepia ink sponge group (CS) and positive control Surgicel Gauze(r) group (SG). Deep second-degree burn model was created in rats. Wound area was recorded by digital imaging and determined using Image J software. Samples were collected and kept at -80oC on 3d, 7d, 14d and 21d for cytokines detecting. Transforming growth factor (TGF)-β1, interleukin (IL)-6, matrix metalloproteinase (MMP)-1, hydroxyproline (Hyp) and macrophage activity reflected by tumor necrosis factor (TNF)-α were determined by enzyme-linked immunosorbent assay (ELISA).RESULTS:
Comparing to Con and SG, scabs in CS group fell off and basically healed on 21 day. TGF-β1, IL-6, MMP-1 and Hyp were significantly increased by CS and SG comparing to Con (p < 0.05), CS had more apparently adjustment on TGF-β1 and MMP-1 compared to SG; results in vitro indicated CS significantly promoted phagocytosis ability of macrophages reflected in TNF-α (p < 0.05).CONCLUSION:
CS improved wound healing through exerting significant influences on secretion of kinds of cytokines and activating macrophages.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Cicatrización de Heridas
/
Quemaduras Químicas
/
Quitina
/
Sepia
/
Macrófagos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Asunto de la revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2016
Tipo del documento:
Article
País de afiliación:
China