Chimaeric G alpha proteins: their potential use in drug discovery.
Trends Pharmacol Sci
; 20(3): 118-24, 1999 Mar.
Article
en En
| MEDLINE
| ID: mdl-10203868
ABSTRACT
Approaches that allow ligand occupancy of a wide range of G protein-coupled receptors to be converted into robust assays amenable to relatively high-throughput analysis are ideal for screening for novel ligands at this class of receptor. Many attempts have been made to design universal ligand-screening systems such that any GPCR can be screened using a common assay end-point. Manipulation of the G protein within the assay system offers the possibility of achieving this. To better understand the domains involved in the interactions between G protein-coupled receptors, G proteins and effector polypeptides and the fine details of these contacts, a wide range of chimaeric G protein alpha subunits have been produced. Graeme Milligan and Stephen Rees discuss the information generated by such studies and the ways in which such chimaeric G proteins can be integrated into assay systems for drug discovery.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes de Fusión
/
Tecnología Farmacéutica
/
Proteínas de Unión al GTP
Idioma:
En
Revista:
Trends Pharmacol Sci
Año:
1999
Tipo del documento:
Article
País de afiliación:
Reino Unido