Pch2 links chromatin silencing to meiotic checkpoint control.
Cell
; 97(3): 313-24, 1999 Apr 30.
Article
en En
| MEDLINE
| ID: mdl-10319812
ABSTRACT
The PCH2 gene of Saccharomyces cerevisiae is required for the meiotic checkpoint that prevents chromosome segregation when recombination and chromosome synapsis are defective. Mutation of PCH2 relieves the checkpoint-induced pachytene arrest of the zip1, zip2, and dmc1 mutants, resulting in chromosome missegregation and low spore viability. Most of the Pch2 protein localizes to the nucleolus, where it represses meiotic interhomolog recombination in the ribosomal DNA, apparently by excluding the meiosis-specific Hop1 protein. Nucleolar localization of Pch2 depends on the silencing factor Sir2, and mutation of SIR2 also bypasses the zip1 pachytene arrest. Under certain circumstances, Sir3-dependent localization of Pch2 to telomeres also provides checkpoint function. These unexpected findings link the nucleolus, chromatin silencing, and the pachytene checkpoint.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Cromatina
/
Proteínas de Ciclo Celular
/
Proteínas de Saccharomyces cerevisiae
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Proteínas Reguladoras de Información Silente de Saccharomyces cerevisiae
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Genes Fúngicos
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Histona Desacetilasas
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Meiosis
Idioma:
En
Revista:
Cell
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos