Impairment of TNF-alpha expression and secretion in primary rat liver cell cultures by acetaminophen treatment.
Toxicology
; 133(2-3): 85-92, 1999 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-10378475
ABSTRACT
Tumor necrosis factor-alpha (TNF-alpha) is assumed to act as a mediator in toxic liver injury, aggravating the primary damage to the parenchymal liver cell, but also stimulating liver regeneration. Reports on the effect of acetaminophen in vivo on TNF-alpha transcript concentrations and serum TNF-alpha concentrations, under different experimental, or clinical conditions have yielded controversial results. We used primary rat hepatocyte and Kupffer cell cultures to test the direct action of subtoxic and toxic concentrations of acetaminophen on TNF-alpha expression and release. We observed a dose-dependent decrease of TNF-alpha mRNA in the hepatocytes, and of TNF-alpha release into the medium of hepatocyte cultures. The data also indicate an impairment of TNF-alpha release in Kupffer cell cultures after treatment with nontoxic, as well as with toxic, acetaminophen concentrations. The results suggest that inhibition of TNF-alpha expression and release in the liver is a consequence of acetaminophen exposure. It is at present unknown how this effect modulates the course of acetaminophen intoxication.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factor de Necrosis Tumoral alfa
/
Analgésicos no Narcóticos
/
Hígado
/
Acetaminofén
Límite:
Animals
Idioma:
En
Revista:
Toxicology
Año:
1999
Tipo del documento:
Article
País de afiliación:
Alemania