Restoration of culturability of starvation-stressed and low-temperature-stressed Escherichia coli O157 cells by using H2O2-degrading compounds.
Arch Microbiol
; 172(1): 63-7, 1999 Jul.
Article
en En
| MEDLINE
| ID: mdl-10398754
ABSTRACT
Late-exponential-phase cells of Escherichia coli O157H- strain E32511/HSC became nonculturable in sterilized distilled water microcosms at 4 degrees C. Plate counts declined from 3 x 10(6) to less than 0.1 CFU/ml in about 21 days. However, when samples of microcosms at 21 days were inoculated onto an agar medium amended with catalase or nonenzyme peroxide-degrading compounds such as sodium pyruvate or alpha-ketoglutaric acid, plate counts increased to 10(4)-10(5) CFU/ml within 48 h. The proposed mode of action of the catalase or pyruvate is via the degradation of the metabolic by-product H2O2, rather than through supplementation of a required nutrient in the recovery of nonculturable cells. Our studies were based on the assumption that E32511/HSC strain responds to starvation and a low temperature by entering a nonculturable state and that the correction of oxidative stress upon the inoculation of bacteria on agar plates promotes recovery of nonculturable cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Escherichia coli O157
/
Peróxido de Hidrógeno
Idioma:
En
Revista:
Arch Microbiol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Japón