Detection and identification of rabies and rabies-related viruses using rapid-cycle PCR.
J Virol Methods
; 81(1-2): 63-9, 1999 Aug.
Article
en En
| MEDLINE
| ID: mdl-10488762
ABSTRACT
The rapid identification of suspect rabies infection is essential in human cases, to ensure appropriate post-exposure treatment of contacts, and in animal cases to allow specific control strategies to be decided and implemented. We describe here the use of high speed air thermo-cycler PCR as a diagnostic tool for the detection of rabies and rabies-related viruses. Using this technique we were able to diagnose rabies in a human within 5 h. Furthermore, the application of automated sequencing of the resultant product enabled a definitive characterisation of classical rabies within 16 h. The utility of this assay was confirmed further by the successful detection of representatives of the six lyssavirus genotypes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Virus de la Rabia
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Virol Methods
Año:
1999
Tipo del documento:
Article
País de afiliación:
Reino Unido