Survival of rats undergoing continuous bile drainage depends on maintenance of circadian rhythm of bile secretion.
Chronobiol Int
; 16(6): 759-65, 1999 Nov.
Article
en En
| MEDLINE
| ID: mdl-10584176
ABSTRACT
It is very difficult to collect bile secretions from animals for extended periods of time. We compared the use of saline or water as drinking fluids to sustain the animals, which were being continuously drained of bile. Complete bile drainage was performed in 16 male Wistar rats by surgical intervention. After surgery, 8 rats were given tap water, and the other 8 were given normal saline for water. The rats that received water rapidly lost weight after bile drainage, and all died within 8 days after the operation. In contrast, all rats that drank saline maintained their body weight and survived 14 days or longer after surgery. Serum biochemistry of the rats with water intake on the third day after bile drainage revealed hyponatremia, hypochloremia, and acute renal failure resulting in hyperkalemia. In contrast, electrolyte balance and renal function were normal in the rats with saline intake, and bile was secreted continuously with a circadian rhythm. These results clearly demonstrate that saline as drinking water is essential to the replacement of lost fluids and loss of electrolytes due to bile drainage. Further, saline proved efficacious for sustaining experimental animals undergoing continuous bile collection.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
2_ODS3
/
6_ODS3_enfermedades_notrasmisibles
Problema de salud:
2_quimicos_contaminacion
/
6_digestive_diseases
/
6_obesity
Asunto principal:
Bilis
/
Ritmo Circadiano
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Chronobiol Int
Asunto de la revista:
FISIOLOGIA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Japón