[Binding sites for A2 and A24 monoclonal antibodies on the alpha-latrotoxin molecule]. / Uchastki uznavaniia monoklonal'nykh antitel A4 i A24 v molekule alpha-latrotoksina.
Bioorg Khim
; 26(4): 299-305, 2000 Apr.
Article
en Ru
| MEDLINE
| ID: mdl-10857022
ABSTRACT
alpha-Latrotoxin (alpha-LTX) binding sites to functionally active monoclonal antibodies (MA) A4 and A24 were localized using three approaches hydrolysis of the toxin followed by the N-terminal sequencing of immunoreactive peptides; the study of antibody interaction with several recombinant alpha-LTX fragments; and Western immunoblotting of synthetic overlapping peptides (6-8 aa) whose structures correspond to that of the immunoreactive alpha-LTX fragment. It was shown that the MA A4 epitope is located within the F234-M294 protein fragment and that of MA A24 interacts with the fragment 347FDKDIT352.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Venenos de Araña
/
Sitios de Unión de Anticuerpos
/
Anticuerpos Monoclonales
Idioma:
Ru
Revista:
Bioorg Khim
Año:
2000
Tipo del documento:
Article
País de afiliación:
Rusia