Different levels of immunoreactivity for synaptosomal-associated protein of 25 kDa in vasoconstrictor and vasodilator axons of guinea-pigs.
Neurosci Lett
; 294(3): 167-70, 2000 Nov 24.
Article
en En
| MEDLINE
| ID: mdl-11072141
ABSTRACT
Immunoreactivity (IR) for synaptosomal-associated protein of 25 kDa (SNAP-25) was examined in axons of autonomic vasoconstrictor and vasodilator neurons innervating the lingual and uterine arteries of guinea-pigs. Polyacrylamide gel electrophoresis and immunoblotting of protein extracts demonstrated a SNAP-25-IR band at 25 kDa in both arteries. Quantitative confocal microscopy demonstrated significantly higher levels of SNAP-25-IR in varicosities with IR for vasoactive intestinal peptide (VIP) than in adjacent axons with IR for tyrosine hydroxylase (TH). Levels of SNAP-25-IR in TH-IR axons, relative to adjacent VIP-IR axons, were significantly higher in the lingual artery than the uterine artery. These differences in IR for SNAP-25, a protein considered essential for calcium-dependent exocytosis of neurotransmitters, raise the possibility that mechanisms of transmitter release may vary between different classes of autonomic neurons.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Axones
/
Tirosina 3-Monooxigenasa
/
Péptido Intestinal Vasoactivo
/
Proteínas de la Membrana
/
Proteínas del Tejido Nervioso
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Año:
2000
Tipo del documento:
Article
País de afiliación:
Australia