Comparative genomic hybridization and cytogenetic analysis in a bronchial adenoma: three clones with different chromosomal aberrations.
Int J Oncol
; 18(5): 923-8, 2001 May.
Article
en En
| MEDLINE
| ID: mdl-11295036
ABSTRACT
Classical chromosomal analysis and comparative genomic hybridization (CGH) were performed in a tubular bronchial gland adenoma. Trisomy of chromosomes 2, 11, 18 and 20 and clonal loss of Y were found in cultured cells derived from two different kryotubes; this was also confirmed by CGH from one of these tubes. Cells from two other tubes, investigated by CGH only, showed gains and losses of parts of chromosome 11q in one, and in the second additional gain of the distal portion of 9q and 17q, respectively. CGH analysis of tumor DNA extracted from paraffin-embedded sections showed no chromosomal imbalances. In cell culture growth the advantage of specific clones probably altered the clone distribution. This study highlights the risk of cytogenetic analysis based on cell cultures only.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de los Bronquios
/
ADN de Neoplasias
/
Adenoma
/
Mapeo Cromosómico
/
Hibridación in Situ
Límite:
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Int J Oncol
Asunto de la revista:
NEOPLASIAS
Año:
2001
Tipo del documento:
Article
País de afiliación:
Austria