Aged mice exhibit in vivo defective peripheral clonal deletion of D(b)/H-Y reactive CD8(+) T cells.
Mech Ageing Dev
; 122(3): 305-26, 2001 Mar.
Article
en En
| MEDLINE
| ID: mdl-11311318
ABSTRACT
We previously reported that T cells from aged mice were resistant to activation-induced cell death (AICD) in vitro. To determine whether the presence of AICD-resistant T cells is associated with defects in age-related peripheral clonal deletion in vivo, congenic male SCID mice were reconstituted with T cells from aged or young female D(b)/H-Y TCR (Tg71) transgenic mice. Compared with recipients of young cells, the recipients of T cells from aged mice exhibited a 3-fold increase in the percentage of autoreactive CD8(+) H-Y antigen-reactive T cells as defined by the clonotypic antibody, M33. There were significantly increased sera levels of interferon-gamma, a significantly decreased expression of FasL by M33(+)CD8(+) T cells, and significantly decreased apoptosis by DNA fragmentation staining of the spleen of mice reconstituted with T cells from aged mice compared to those from young mice. By day 21, the recipients of T cells from aged mice but not young mice, exhibited infiltration of CD3(+) cells into the non-lymphoid organs. These results indicate that there is defective peripheral deletion of the self-reactive T cells derived from aged female Tg71 mice, and that failure to delete these cells is associated with the defective T-cell clonal deletion in the recipient mice.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Envejecimiento
/
Antígenos H-2
/
Antígeno H-Y
/
Linfocitos T CD8-positivos
/
Complejo Mayor de Histocompatibilidad
Límite:
Animals
Idioma:
En
Revista:
Mech Ageing Dev
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos