Isolation and characterization of a human thiamine pyrophosphokinase cDNA.
Biochim Biophys Acta
; 1517(2): 293-7, 2001 Jan 26.
Article
en En
| MEDLINE
| ID: mdl-11342111
ABSTRACT
A human thiamine pyrophosphokinase cDNA clone (hTPK1) was isolated and sequenced. When the intact hTPK1 open reading frame was expressed as a histidine-tag fusion protein in Escherichia coli, marked enzyme activity was detected in the bacterial cells. The hTPK1 mRNA was widely expressed in various human tissues at a very low level, and the mRNA content in cultured fibroblasts was unaffected by the thiamine concentration of the medium. The chromosome localization of the hTPK1 gene was assigned to 7q34.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
ADN Complementario
/
Tiamina Pirofosfoquinasa
Límite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón