Isotype specific immune responses in murine experimental toxocariasis.
Mem Inst Oswaldo Cruz
; 96(4): 549-53, 2001 May.
Article
en En
| MEDLINE
| ID: mdl-11391430
ABSTRACT
In this work, a murine experimental model of toxocariasis has been developed in BALB/c, C57BL/10 and C3H murine strains orally inoculated with 4,000 Toxocara canis embryonated eggs, in order to investigate the isotype-specific immune responses against excretory-secretory antigens from larvae. T. canis specific IgG+M, IgM, IgG, IgA, IgG1, IgG2a and IgG3 were tested by ELISA. The dynamics of the specific immunoglobulins (IgG+IgM) production showed a contrasting profile regarding the murine strain. Conversely to the results obtained with the IgM isotype, the IgG antibody class showed similar patterns to those obtained with IgG+IgM antibodies, only in the case of the BALB/c strain, being different and much higher than the obtained with IgG+IgM antibodies, when the C3H murine strain was used. The antibodies IgG+IgM tested in BALB/c and C57BL/10 were both of the IgM and IgG isotypes. Conversely, in the C3H strain only IgG specific antibody levels were detected. The IgG1 subclass responses showed a similar profile in the three murine strains studied, with high values in BALB/c, as in the case of the IgG responses.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Isotipos de Inmunoglobulinas
/
Anticuerpos Antihelmínticos
/
Toxocariasis
/
Toxocara canis
Límite:
Animals
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Año:
2001
Tipo del documento:
Article