Simulation of endo-PG digest patterns and implications for the determination of pectin fine structure.
Carbohydr Res
; 334(3): 243-50, 2001 Aug 30.
Article
en En
| MEDLINE
| ID: mdl-11513831
ABSTRACT
Novel stepwise approaches to the calculation of enzyme digest patterns are described and used in the validation of a computer simulation. Results obtained using the simulation show that, while a previously proposed model of endo-PG action captures some of the salient features of this enzymes behaviour, it is not sufficient to successfully predict experimental digest patterns from pectic substrates. Subsequently, it has been shown that a modified model incorporating existing information regarding subsite architecture and speculative site tolerances for esterified residues, goes someway towards improving the situation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Poligalacturonasa
/
Simulación por Computador
/
Pectinas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Carbohydr Res
Año:
2001
Tipo del documento:
Article
País de afiliación:
Reino Unido