Erythrocyte spectrin is an E2 ubiquitin conjugating enzyme.
Biochemistry
; 40(38): 11630-42, 2001 Sep 25.
Article
en En
| MEDLINE
| ID: mdl-11560514
ABSTRACT
The involvement of red blood cell spectrin in the ubiquitination process was studied. Spectrin was found to form two ubiquitin-associated derivatives, a DTT-sensitive ubiquitin adduct and a DTT-insensitive conjugate, characteristic intermediate and final products of the ubiquitination reaction cascade. In addition to spectrin and ubiquitin, ubiquitin-activating enzyme (E1) and ATP were necessary and sufficient to form both the spectrin-ubiquitin adduct and conjugate. No exogenous ubiquitin-conjugating (E2) or ligase (E3) activities were required, suggesting that erythrocyte spectrin is an E2 ubiquitin-conjugating enzyme able to target itself. Both ubiquitin adduct and conjugate were linked to the alpha subunit of spectrin, suggesting that the ubiquitin-conjugating (UBC) domain and its target regions reside on the same subunit.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ubiquitinas
/
Espectrina
/
Membrana Eritrocítica
/
Ligasas
Límite:
Humans
Idioma:
En
Revista:
Biochemistry
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos